##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2942
Sequences flagged as poor quality	0
Sequence length	40
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.157036029911623	32.0	29.0	34.0	4.0	37.0
2	24.270904146838884	28.0	20.0	33.0	4.0	36.0
3	20.794697484704283	23.0	13.0	29.0	4.0	33.0
4	22.238953093133922	26.0	12.0	32.0	4.0	35.0
5	18.08089734874235	19.0	10.0	26.0	4.0	32.0
6	18.877294357579878	17.0	11.0	30.0	4.0	35.0
7	23.7324949014276	28.0	18.0	33.0	4.0	36.0
8	22.926920462270566	27.0	11.0	33.0	4.0	36.0
9	23.382732834806255	27.0	14.0	33.0	4.0	36.0
10	19.274983004758667	20.0	7.0	29.0	4.0	34.0
11	21.53467029231815	24.0	8.0	32.0	4.0	36.0
12	21.64174031271244	25.0	7.0	32.0	4.0	36.0
13	22.447994561522773	27.0	4.0	33.0	4.0	36.0
14	22.559483344663494	27.0	4.0	33.0	4.0	36.0
15	20.492182188987083	23.0	4.0	32.0	4.0	35.0
16	20.34840244731475	24.0	4.0	32.0	4.0	36.0
17	21.099592114208022	26.0	4.0	33.0	4.0	36.0
18	20.671312032630862	25.0	4.0	33.0	4.0	36.0
19	17.963630183548606	18.0	4.0	30.0	4.0	34.0
20	19.720258327668255	23.0	4.0	33.0	4.0	36.0
21	16.389191026512577	14.0	4.0	29.0	4.0	34.0
22	16.57205982324949	14.0	4.0	30.0	4.0	34.0
23	16.3956492182189	13.0	4.0	30.0	4.0	34.0
24	16.184568320870156	11.0	4.0	30.0	4.0	34.0
25	15.491502379333786	4.0	4.0	30.0	4.0	34.0
26	12.769204622705642	4.0	4.0	24.0	4.0	33.0
27	12.368796736913664	4.0	4.0	23.0	4.0	33.0
28	12.160774983004758	4.0	4.0	23.0	4.0	32.0
29	11.607409925220939	4.0	4.0	21.0	4.0	32.0
30	10.601291638341264	4.0	4.0	17.0	4.0	31.0
31	10.754928619986403	4.0	4.0	18.0	4.0	31.0
32	10.254588715159755	4.0	4.0	15.0	4.0	31.0
33	10.266485384092453	4.0	4.0	15.0	4.0	31.0
34	9.898368456832086	4.0	4.0	12.0	4.0	30.0
35	9.747450713800136	4.0	4.0	5.0	4.0	30.0
36	9.5462270564242	4.0	4.0	4.0	4.0	32.0
37	9.716859279401767	4.0	4.0	4.0	4.0	32.0
38	9.217199184228416	4.0	4.0	4.0	4.0	31.0
39	9.0350101971448	4.0	4.0	4.0	4.0	31.0
40	8.930659415363698	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-14.736374351720883
1	2	-18.204537953795374
1	3	-14.64007543611504
1	4	-15.783227251296562
1	5	-12.404101838755302
1	6	-12.249516737388024
1	7	-16.879184347006127
1	8	-16.220520980669498
1	9	-16.846841112682696
1	10	-12.219495520980672
1	11	-15.728830740216882
1	12	-15.54634606317775
1	13	-16.07362093352193
1	14	-15.674599245638845
1	15	-14.073727015558699
1	16	-14.369071192833573
1	17	-14.328217821782179
1	18	-14.225388967468177
1	19	-11.578960396039603
1	20	-13.387010843941539
1	21	-10.078064592173504
1	22	-9.774787835926452
1	23	-9.639509665252243
1	24	-9.483050447901933
1	25	-9.805964167845358
1	26	-7.844295143800096
1	27	-7.6084040546911815
1	28	-7.47887788778878
1	29	-6.929903347477605
1	30	-6.049033474776049
1	31	-6.3720768505421965
1	32	-6.322536539368221
1	33	-5.7904290429042895
1	34	-5.688036303630364
1	35	-5.886468646864689
1	36	-5.420497406883545
1	37	-5.026638378123527
1	38	-4.989910419613391
1	39	-4.557732201791609
1	40	-4.581494578029233
4	1	-13.926850542197075
4	2	-11.87120462046204
4	3	-12.973408769448373
4	4	-7.116560584629896
4	5	-12.404101838755302
4	6	-12.249516737388024
4	7	-16.879184347006127
4	8	-16.220520980669498
4	9	-16.846841112682696
4	10	-12.219495520980672
4	11	-15.728830740216882
4	12	-15.54634606317775
4	13	-16.07362093352193
4	14	-15.674599245638845
4	15	-14.073727015558699
4	16	-14.369071192833573
4	17	-14.328217821782179
4	18	-14.225388967468177
4	19	-11.578960396039603
4	20	-13.387010843941539
4	21	-10.078064592173504
4	22	-9.774787835926452
4	23	-9.639509665252243
4	24	-9.483050447901933
4	25	-9.805964167845358
4	26	-7.844295143800096
4	27	-7.6084040546911815
4	28	-7.47887788778878
4	29	-6.929903347477605
4	30	-6.049033474776049
4	31	-6.3720768505421965
4	32	-6.322536539368221
4	33	-5.7904290429042895
4	34	-5.688036303630364
4	35	-5.886468646864689
4	36	-5.420497406883545
4	37	-5.026638378123527
4	38	-4.989910419613391
4	39	-4.557732201791609
4	40	-4.581494578029233
5	1	-9.84351720886374
5	2	-10.454537953795374
5	3	-5.8900754361150405
5	4	-9.783227251296562
5	5	-5.404101838755302
5	6	-7.749516737388024
5	7	-7.629184347006127
5	8	-6.470520980669498
5	9	-12.596841112682696
5	10	-5.469495520980672
5	11	-5.978830740216882
5	12	-6.046346063177751
5	13	-4.82362093352193
5	14	-4.924599245638845
5	15	-4.823727015558699
5	16	2.630928807166427
5	17	2.6717821782178213
5	18	2.7746110325318227
5	19	1.4210396039603967
5	20	3.112989156058461
5	21	-1.5780645921735044
5	22	-1.2747878359264515
5	23	-1.139509665252243
5	24	-0.9830504479019329
5	25	-1.3059641678453584
5	26	0.9057048561999039
5	27	1.1415959453088185
5	28	1.27112211221122
5	29	1.8200966525223947
5	30	2.7009665252239508
5	31	1.3779231494578035
5	32	1.4274634606317793
5	33	2.7095709570957105
5	34	1.3119636963696362
5	35	2.6135313531353113
5	36	3.0795025931164552
5	37	3.4733616218764727
5	38	2.760089580386609
5	39	3.192267798208391
5	40	2.4185054219707673
6	1	-0.5935172088637408
6	2	2.4621287128712943
6	3	2.0265912305516274
6	4	0.8834394153701055
6	5	-2.7374351720886363
6	6	-4.91618340405469
6	7	-7.212517680339461
6	8	-3.887187647336166
6	9	-3.8468411126826965
6	10	-4.886162187647338
6	11	-8.728830740216882
6	12	-6.213012729844417
6	13	-6.406954266855264
6	14	-10.007932578972179
6	15	-5.073727015558699
6	16	-5.702404526166907
6	17	-1.3282178217821787
6	18	-4.892055634134843
6	19	2.4210396039603967
6	20	-1.7203441772748729
6	21	-1.4113979255068383
6	22	5.891878830740216
6	23	-3.639509665252243
6	24	2.183616218764733
6	25	5.8607024988213094
6	26	11.822371522866572
6	27	-0.2750707213578476
6	28	6.187788778877888
6	29	3.736763319189061
6	30	-6.049033474776049
6	31	-6.3720768505421965
6	32	-6.322536539368221
6	33	-5.7904290429042895
6	34	-5.688036303630364
6	35	-5.886468646864689
6	36	-5.420497406883545
6	37	-5.026638378123527
6	38	-4.989910419613391
6	39	-4.557732201791609
6	40	-4.581494578029233
7	1	8.65648279113626
7	2	12.795462046204626
7	3	9.10992456388496
7	4	8.716772748703438
7	5	10.595898161244698
7	6	16.000483262611976
7	7	13.870815652993873
7	8	12.529479019330502
7	9	13.903158887317304
7	10	18.530504479019328
7	11	15.771169259783118
7	12	5.953653936822249
7	13	12.42637906647807
7	14	13.325400754361155
7	15	14.926272984441301
7	16	16.380928807166427
7	17	15.921782178217821
7	18	11.524611032531823
7	19	16.921039603960395
7	20	13.862989156058461
7	21	14.421935407826496
7	22	17.725212164073547
7	23	20.11049033474776
7	24	18.76694955209807
7	25	7.944035832154642
7	26	0.9057048561999039
7	27	-6.1084040546911815
7	28	-0.47887788778878004
7	29	-0.17990334747760528
7	30	0.7009665252239508
7	31	-0.3720768505421965
7	32	1.1774634606317793
7	33	-5.7904290429042895
7	34	-5.688036303630364
7	35	-5.886468646864689
7	36	-5.420497406883545
7	37	-5.026638378123527
7	38	-4.989910419613391
7	39	-4.557732201791609
7	40	-4.581494578029233
9	1	2.7398161244695913
9	2	5.128795379537959
9	3	3.5265912305516274
9	4	8.216772748703438
9	5	2.2625648279113655
9	6	2.083816595945308
9	7	10.28748231966054
9	8	10.612812352663834
9	9	8.986492220650636
9	10	9.280504479019328
9	11	7.271169259783118
9	12	8.286987270155581
9	13	7.92637906647807
9	14	0.6587340876944872
9	15	4.592939651107969
9	16	7.797595473833095
9	17	12.83844884488449
9	18	11.607944365865155
9	19	9.254372937293729
9	20	11.612989156058461
9	21	17.421935407826496
9	22	5.891878830740216
9	23	11.527157001414425
9	24	14.516949552098067
9	25	14.194035832154642
9	26	5.489038189533236
9	27	7.3915959453088185
9	28	2.687788778877886
9	29	4.236763319189061
9	30	3.4509665252239508
9	31	4.461256482791137
9	32	5.177463460631779
9	33	4.876237623762377
9	34	5.64529702970297
9	35	6.280198019801979
9	36	6.412835926449789
9	37	8.306694955209805
9	38	5.843422913719943
9	39	6.608934464875057
9	40	5.418505421970767
10	1	2.5493399339934015
10	2	6.081176331918911
10	3	9.074210278170675
10	4	9.502487034417722
10	5	6.024469589816125
10	6	8.607626119754833
10	7	9.263672795851015
10	8	8.208050447901929
10	9	9.58173031588873
10	10	7.0662187647336125
10	11	11.699740688354545
10	12	11.167939651107964
10	13	10.212093352192355
10	14	10.896829325789728
10	15	6.497701555869874
10	16	11.630928807166427
10	17	9.100353606789248
10	18	12.20318246110325
10	19	11.849611032531824
10	20	13.041560584629888
10	21	7.493363979255069
10	22	11.939497878359264
10	23	15.217633191890615
10	24	14.08837812352664
10	25	7.479750117868926
10	26	4.012847713342762
10	27	6.963024516737391
10	28	6.949693540782647
10	29	7.498668081093822
10	30	2.3795379537953796
10	31	5.056494578029232
10	32	-2.6082508250825063
10	33	-5.7904290429042895
10	34	-5.688036303630364
10	35	-5.886468646864689
10	36	-5.420497406883545
10	37	-5.026638378123527
10	38	-4.989910419613391
10	39	-4.557732201791609
10	40	-4.581494578029233
11	1	5.739816124469595
11	2	7.462128712871294
11	3	7.026591230551627
11	4	7.216772748703438
11	5	7.2625648279113655
11	6	4.750483262611976
11	7	4.7874823196605405
11	8	11.112812352663834
11	9	6.486492220650636
11	10	3.780504479019328
11	11	11.937835926449786
11	12	4.120320603488917
11	13	10.259712399811402
11	14	12.65873408769449
11	15	7.926272984441301
11	16	16.630928807166427
11	17	16.67178217821782
11	18	16.774611032531823
11	19	15.754372937293729
11	20	16.61298915605846
11	21	19.921935407826496
11	22	15.55854549740688
11	23	22.36049033474776
11	24	12.183616218764735
11	25	12.527369165487974
11	26	14.155704856199904
11	27	3.0582626119754845
11	28	14.854455445544552
11	29	14.403429985855727
11	30	15.95096652522395
11	31	12.627923149457803
11	32	12.67746346063178
11	33	10.542904290429043
11	34	11.645297029702968
11	35	11.78019801980198
11	36	-5.420497406883545
11	37	-5.026638378123527
11	38	-4.989910419613391
11	39	-4.557732201791609
11	40	-4.581494578029233
12	1	1.20648279113626
12	2	5.395462046204628
12	3	1.759924563884958
12	4	8.016772748703438
12	5	3.795898161244697
12	6	-0.44951673738802356
12	7	6.520815652993871
12	8	9.979479019330501
12	9	6.553158887317302
12	10	4.780504479019328
12	11	11.271169259783118
12	12	4.853653936822248
12	13	10.92637906647807
12	14	10.925400754361156
12	15	11.1262729844413
12	16	14.630928807166427
12	17	16.271782178217823
12	18	14.374611032531824
12	19	15.621039603960396
12	20	11.81298915605846
12	21	10.921935407826496
12	22	13.425212164073548
12	23	11.560490334747756
12	24	18.11694955209807
12	25	12.994035832154642
12	26	7.5557048561999025
12	27	-1.6084040546911815
12	28	9.72112211221122
12	29	3.8700966525223954
12	30	4.350966525223951
12	31	5.227923149457803
12	32	-1.3225365393682207
12	33	-0.5904290429042902
12	34	-0.6880363036303638
12	35	-0.8864686468646887
12	36	-3.2204974068835446
12	37	0.5733616218764723
12	38	0.6100895803866084
12	39	1.0422677982083908
12	40	-0.1814945780292323
13	1	-3.093517208863741
13	2	-5.4545379537953735
13	3	-4.6400754361150405
13	4	-7.533227251296562
13	5	-7.154101838755302
13	6	6.000483262611976
13	7	-6.879184347006127
13	8	-2.220520980669498
13	9	-2.5968411126826965
13	10	-2.719495520980672
13	11	2.271169259783118
13	12	4.953653936822249
13	13	0.67637906647807
13	14	0.575400754361155
13	15	3.426272984441301
13	16	-0.619071192833573
13	17	5.421782178217821
13	18	2.5246110325318227
13	19	-4.078960396039603
13	20	-0.637010843941539
13	21	-3.3280645921735044
13	22	-3.7747878359264515
13	23	-3.639509665252243
13	24	-2.233050447901933
13	25	-2.5559641678453584
13	26	-7.844295143800096
13	27	-7.6084040546911815
13	28	-7.47887788778878
13	29	-6.929903347477605
13	30	-6.049033474776049
13	31	-6.3720768505421965
13	32	-6.322536539368221
13	33	-5.7904290429042895
13	34	-5.688036303630364
13	35	-5.886468646864689
13	36	-5.420497406883545
13	37	-5.026638378123527
13	38	-4.989910419613391
13	39	-4.557732201791609
13	40	-4.581494578029233
15	1	2.656482791136259
15	2	4.9204620462046265
15	3	7.1099245638849595
15	4	6.091772748703438
15	5	6.595898161244698
15	6	5.875483262611976
15	7	7.870815652993873
15	8	6.904479019330502
15	9	7.1531588873173035
15	10	5.030504479019328
15	11	7.271169259783118
15	12	6.953653936822249
15	13	8.92637906647807
15	14	8.700400754361155
15	15	3.301272984441301
15	16	10.130928807166427
15	17	5.671782178217821
15	18	6.774611032531823
15	19	9.796039603960397
15	20	9.612989156058461
15	21	4.296935407826496
15	22	5.6002121640735485
15	23	8.110490334747757
15	24	7.766949552098067
15	25	6.944035832154642
15	26	3.405704856199904
15	27	3.7665959453088185
15	28	0.27112211221121996
15	29	0.4450966525223947
15	30	1.4509665252239508
15	31	0.2529231494578035
15	32	-1.0725365393682207
15	33	-0.5404290429042895
15	34	0.43696369636963617
15	35	-0.6364686468646887
15	36	-1.2954974068835448
15	37	-1.5266383781235273
15	38	-0.2399104196133912
15	39	-0.5577322017916089
15	40	0.9185054219707673
16	1	2.20648279113626
16	2	6.395462046204628
16	3	4.559924563884959
16	4	0.8167727487034391
16	5	0.39589816124469834
16	6	-0.8495167373880239
16	7	1.1208156529938726
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108	11	16.271169259783118
108	12	16.45365393682225
108	13	14.92637906647807
108	14	17.825400754361155
108	15	15.926272984441301
108	16	17.130928807166427
108	17	16.67178217821782
108	18	12.774611032531823
108	19	-5.578960396039603
108	20	1.112989156058461
108	21	-10.078064592173504
108	22	-9.774787835926452
108	23	-9.639509665252243
108	24	-9.483050447901933
108	25	-9.805964167845358
108	26	-7.844295143800096
108	27	-7.6084040546911815
108	28	-7.47887788778878
108	29	-6.929903347477605
108	30	-6.049033474776049
108	31	-6.3720768505421965
108	32	-6.322536539368221
108	33	-5.7904290429042895
108	34	-5.688036303630364
108	35	-5.886468646864689
108	36	-5.420497406883545
108	37	-5.026638378123527
108	38	-4.989910419613391
108	39	-4.557732201791609
108	40	-4.581494578029233
109	1	-0.5935172088637408
109	2	3.2954620462046265
109	3	-2.1400754361150405
109	4	-1.2832272512965623
109	5	-7.404101838755302
109	6	-0.7495167373880243
109	7	4.620815652993873
109	8	3.279479019330502
109	9	3.6531588873173035
109	10	-6.219495520980672
109	11	10.771169259783118
109	12	11.45365393682225
109	13	-3.57362093352193
109	14	-2.174599245638845
109	15	-1.573727015558699
109	16	-3.869071192833573
109	17	2.6717821782178213
109	18	2.7746110325318227
109	19	5.421039603960397
109	20	3.612989156058461
109	21	6.921935407826496
109	22	2.7252121640735485
109	23	7.360490334747757
109	24	7.516949552098067
109	25	7.194035832154642
109	26	8.655704856199904
109	27	7.3915959453088185
109	28	9.02112211221122
109	29	9.070096652522395
109	30	8.95096652522395
109	31	-6.3720768505421965
109	32	-6.322536539368221
109	33	-5.7904290429042895
109	34	-5.688036303630364
109	35	-5.886468646864689
109	36	-5.420497406883545
109	37	-5.026638378123527
109	38	-4.989910419613391
109	39	-4.557732201791609
109	40	-4.581494578029233
110	1	-4.593517208863741
110	2	-5.537871287128706
110	3	-3.9734087694483744
110	4	-4.116560584629896
110	5	-3.4041018387553024
110	6	-5.582850070721358
110	7	-3.2125176803394595
110	8	-4.887187647336164
110	9	-1.1801744460160286
110	10	3.780504479019328
110	11	-0.06216407355021403
110	12	3.120320603488917
110	13	-1.7402876001885978
110	14	2.658734087694487
110	15	2.259606317774633
110	16	4.297595473833095
110	17	3.6717821782178213
110	18	0.7746110325318227
110	19	-9.24562706270627
110	20	2.612989156058461
110	21	-2.4113979255068383
110	22	5.891878830740216
110	23	-5.639509665252243
110	24	2.516949552098067
110	25	4.527369165487974
110	26	7.155704856199904
110	27	5.724929278642151
110	28	-1.47887788778878
110	29	0.07009665252239472
110	30	2.9509665252239508
110	31	1.9612564827911374
110	32	0.3441301272984454
110	33	-2.1237623762376225
110	34	0.9786303630363022
110	35	1.4468646864686452
110	36	3.2461692597831213
110	37	-5.026638378123527
110	38	-4.989910419613391
110	39	-4.557732201791609
110	40	-4.581494578029233
111	1	3.406482791136259
111	2	1.2954620462046265
111	3	4.8599245638849595
111	4	3.7167727487034377
111	5	7.595898161244698
111	6	-6.749516737388024
111	7	-5.379184347006127
111	8	-8.720520980669498
111	9	-5.3468411126826965
111	10	-6.719495520980672
111	11	-2.728830740216882
111	12	-4.546346063177751
111	13	-7.07362093352193
111	14	-5.674599245638845
111	15	-6.073727015558699
111	16	-1.869071192833573
111	17	0.17178217821782127
111	18	0.7746110325318227
111	19	0.42103960396039675
111	20	1.612989156058461
111	21	-5.078064592173504
111	22	4.7252121640735485
111	23	3.860490334747757
111	24	4.016949552098067
111	25	1.1940358321546416
111	26	9.655704856199904
111	27	9.891595945308818
111	28	10.02112211221122
111	29	7.070096652522395
111	30	11.45096652522395
111	31	10.627923149457803
111	32	10.67746346063178
111	33	9.70957095709571
111	34	11.311963696369636
111	35	11.113531353135311
111	36	1.0795025931164552
111	37	1.4733616218764727
111	38	10.010089580386609
111	39	1.9422677982083911
111	40	1.9185054219707673
112	1	-10.34351720886374
112	2	-14.954537953795374
112	3	-5.6400754361150405
112	4	-3.2832272512965623
112	5	-4.404101838755302
112	6	-7.249516737388024
112	7	-8.129184347006127
112	8	-8.220520980669498
112	9	-5.5968411126826965
112	10	-6.719495520980672
112	11	-4.228830740216882
112	12	-4.796346063177751
112	13	-6.07362093352193
112	14	-1.924599245638845
112	15	-2.573727015558699
112	16	-1.869071192833573
112	17	-1.3282178217821787
112	18	-5.225388967468177
112	19	-3.8289603960396033
112	20	0.612989156058461
112	21	-4.078064592173504
112	22	-2.5247878359264515
112	23	-3.639509665252243
112	24	-6.733050447901933
112	25	-5.055964167845358
112	26	-1.094295143800096
112	27	-0.10840405469118153
112	28	-2.97887788778878
112	29	-5.679903347477605
112	30	1.4509665252239508
112	31	-0.8720768505421965
112	32	-0.8225365393682207
112	33	1.4595709570957105
112	34	0.31196369636963617
112	35	1.8635313531353113
112	36	-5.420497406883545
112	37	-5.026638378123527
112	38	-4.989910419613391
112	39	-4.557732201791609
112	40	-4.581494578029233
114	1	8.40648279113626
114	2	9.795462046204626
114	3	13.60992456388496
114	4	14.716772748703438
114	5	16.345898161244698
114	6	3.5004832626119757
114	7	11.620815652993873
114	8	13.529479019330502
114	9	9.653158887317304
114	10	14.780504479019328
114	11	-1.9788307402168819
114	12	4.953653936822249
114	13	9.67637906647807
114	14	12.075400754361155
114	15	15.176272984441301
114	16	8.130928807166427
114	17	10.921782178217821
114	18	10.024611032531823
114	19	12.921039603960397
114	20	10.362989156058461
114	21	16.921935407826496
114	22	7.4752121640735485
114	23	2.610490334747757
114	24	7.016949552098067
114	25	-3.0559641678453584
114	26	-7.844295143800096
114	27	-7.6084040546911815
114	28	-7.47887788778878
114	29	-6.929903347477605
114	30	-6.049033474776049
114	31	-6.3720768505421965
114	32	-6.322536539368221
114	33	-5.7904290429042895
114	34	-5.688036303630364
114	35	-5.886468646864689
114	36	-5.420497406883545
114	37	-5.026638378123527
114	38	-4.989910419613391
114	39	-4.557732201791609
114	40	-4.581494578029233
115	1	3.073149457802927
115	2	5.7954620462046265
115	3	8.35992456388496
115	4	5.55010608203677
115	5	5.595898161244698
115	6	-7.582850070721358
115	7	-4.2125176803394595
115	8	0.44614568599716975
115	9	-9.513507779349363
115	10	-3.552828854314006
115	11	-0.39549740688354973
115	12	-0.879679396511083
115	13	-0.40695426685526215
115	14	0.6587340876944872
115	15	3.5929396511079688
115	16	-9.702404526166907
115	17	-4.328217821782179
115	18	-4.558722300801511
115	19	-2.912293729372937
115	20	-3.387010843941539
115	21	-10.078064592173504
115	22	-9.774787835926452
115	23	-9.639509665252243
115	24	-9.483050447901933
115	25	-9.805964167845358
115	26	-7.844295143800096
115	27	-7.6084040546911815
115	28	-7.47887788778878
115	29	-6.929903347477605
115	30	-6.049033474776049
115	31	-6.3720768505421965
115	32	-6.322536539368221
115	33	-5.7904290429042895
115	34	-5.688036303630364
115	35	-5.886468646864689
115	36	-5.420497406883545
115	37	-5.026638378123527
115	38	-4.989910419613391
115	39	-4.557732201791609
115	40	-4.581494578029233
116	1	2.406482791136259
116	2	6.7954620462046265
116	3	-2.6400754361150405
116	4	-6.783227251296562
116	5	-6.404101838755302
116	6	-5.249516737388024
116	7	9.120815652993873
116	8	1.779479019330502
116	9	8.153158887317304
116	10	4.780504479019328
116	11	15.271169259783118
116	12	8.45365393682225
116	13	7.92637906647807
116	14	10.325400754361155
116	15	10.926272984441301
116	16	12.630928807166427
116	17	12.671782178217821
116	18	-14.225388967468177
116	19	-11.578960396039603
116	20	-13.387010843941539
116	21	-10.078064592173504
116	22	-9.774787835926452
116	23	-9.639509665252243
116	24	-9.483050447901933
116	25	-9.805964167845358
116	26	-7.844295143800096
116	27	-7.6084040546911815
116	28	-7.47887788778878
116	29	-6.929903347477605
116	30	-6.049033474776049
116	31	-6.3720768505421965
116	32	-6.322536539368221
116	33	-5.7904290429042895
116	34	-5.688036303630364
116	35	-5.886468646864689
116	36	-5.420497406883545
116	37	-5.026638378123527
116	38	-4.989910419613391
116	39	-4.557732201791609
116	40	-4.581494578029233
120	1	8.40648279113626
120	2	12.795462046204626
120	3	14.35992456388496
120	4	15.216772748703438
120	5	16.595898161244698
120	6	-8.249516737388024
120	7	-7.879184347006127
120	8	14.779479019330502
120	9	12.153158887317304
120	10	10.780504479019328
120	11	11.271169259783118
120	12	-15.54634606317775
120	13	-16.07362093352193
120	14	-15.674599245638845
120	15	-14.073727015558699
120	16	-14.369071192833573
120	17	-14.328217821782179
120	18	-14.225388967468177
120	19	-11.578960396039603
120	20	-13.387010843941539
120	21	-10.078064592173504
120	22	-9.774787835926452
120	23	-9.639509665252243
120	24	-9.483050447901933
120	25	-9.805964167845358
120	26	-7.844295143800096
120	27	-7.6084040546911815
120	28	-7.47887788778878
120	29	-6.929903347477605
120	30	-6.049033474776049
120	31	-6.3720768505421965
120	32	-6.322536539368221
120	33	-5.7904290429042895
120	34	-5.688036303630364
120	35	-5.886468646864689
120	36	-5.420497406883545
120	37	-5.026638378123527
120	38	-4.989910419613391
120	39	-4.557732201791609
120	40	-4.581494578029233
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	554.0
5	27.0
6	37.0
7	28.0
8	20.0
9	38.0
10	57.0
11	56.0
12	96.0
13	105.0
14	127.0
15	116.0
16	117.0
17	139.0
18	123.0
19	136.0
20	153.0
21	126.0
22	141.0
23	137.0
24	109.0
25	102.0
26	89.0
27	85.0
28	77.0
29	53.0
30	45.0
31	18.0
32	14.0
33	8.0
34	5.0
35	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.04632152588556	0.6130790190735695	0.2724795640326975	0.06811989100817438
8	97.71953710006808	0.7147719537100068	0.6466984343090538	0.9189925119128658
9	97.68471229145386	1.2257405515832482	0.4766768811712632	0.6128702757916241
10	38.27328348062542	29.333786539768862	21.58395649218219	10.808973487423522
11	27.736233854520737	39.496940856560165	18.524813052345344	14.242012236573759
12	31.4858891533492	15.36892213532812	20.673240394423665	32.47194831689902
13	33.92250169952413	16.315431679129844	23.827328348062544	25.934738273283482
14	20.36029911624745	23.861318830727395	25.730795377294356	30.0475866757308
15	25.866757307953776	29.74167233174711	25.458871515975527	18.93269884432359
16	29.027872195785182	19.03467029231815	21.34602311352821	30.591434398368456
17	25.356900067980963	31.475186947654656	21.07409925220938	22.093813732154995
18	35.31611148878314	21.889870836165873	23.55540448674371	19.238613188307273
19	21.509047456469784	15.090474564697848	26.83509730283373	36.56538067599863
20	21.78789938817131	24.371176070700205	32.426920462270566	21.41400407885792
21	23.419442556084295	16.859279401767505	27.090414683888508	32.63086335825969
22	19.748470428280083	17.2331747110809	32.56288239292998	30.455472467709043
23	31.475186947654656	20.87015635622026	25.084976206662134	22.56968048946295
24	21.006118286879673	29.707681849082256	24.7450713800136	24.541128484024473
25	32.53995239714383	19.82318939136348	24.107446446786806	23.52941176470588
26	17.876712328767123	20.171232876712327	34.863013698630134	27.08904109589041
27	17.07482993197279	17.210884353741495	27.346938775510203	38.36734693877551
28	25.84155049302958	18.80312818769126	26.181570894253653	29.173750425025503
29	28.31858407079646	17.903335602450646	29.339686861810755	24.438393464942138
30	17.075664621676893	23.721881390593047	32.89025221540559	26.31220177232447
31	27.05281090289608	19.38671209540034	33.015332197614995	20.545144804088586
32	20.979258755525333	23.93743624617477	31.58789527371642	23.495409724583475
33	15.94153636981645	16.00951733514616	33.17471108089735	34.87423521414004
34	13.426240652617267	25.186947654656695	35.8599592114208	25.526852481305234
35	14.242012236573759	17.743031951053705	38.57919782460911	29.435757987763427
36	21.821889870836166	18.762746430999318	35.21414004078858	24.201223657375934
37	14.246854811288678	17.987079224753487	36.48418905134308	31.28187691261476
38	17.27303638218293	21.217273036382185	34.308058483509015	27.201632097925877
39	25.084976206662134	18.93269884432359	33.10673011556764	22.875594833446637
40	23.657375934738273	18.558803535010195	36.16587355540449	21.617946974847044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	0.5
15	0.0
16	2.5
17	5.0
18	5.0
19	10.0
20	15.0
21	20.5
22	26.0
23	26.0
24	29.5
25	33.0
26	40.5
27	48.0
28	48.0
29	47.0
30	46.0
31	55.5
32	65.0
33	65.0
34	69.0
35	73.0
36	94.0
37	115.0
38	115.0
39	133.5
40	152.0
41	136.0
42	120.0
43	120.0
44	134.5
45	149.0
46	141.5
47	134.0
48	134.0
49	149.0
50	164.0
51	170.0
52	176.0
53	176.0
54	171.5
55	167.0
56	195.5
57	224.0
58	224.0
59	250.0
60	276.0
61	295.5
62	315.0
63	315.0
64	280.0
65	245.0
66	207.0
67	169.0
68	169.0
69	135.5
70	102.0
71	80.0
72	58.0
73	58.0
74	47.0
75	36.0
76	27.0
77	18.0
78	18.0
79	11.5
80	5.0
81	4.0
82	3.0
83	3.0
84	2.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.20394289598912305
8	0.13596193065941536
9	0.1699524133242692
10	0.0
11	0.0
12	0.03399048266485384
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.4418762746430999
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.03399048266485384
26	0.7477906186267845
27	0.06798096532970768
28	0.03399048266485384
29	0.13596193065941536
30	0.27192386131883073
31	0.2379333786539769
32	0.03399048266485384
33	0.0
34	0.0
35	0.0
36	0.0
37	0.03399048266485384
38	0.03399048266485384
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2942.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.70632222977567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.92290585618977	87.96736913664174
2	2.705707931801334	4.962610469068661
3	0.6671608598962194	1.8354860639021073
4	0.3335804299481097	1.2236573759347382
5	0.14825796886582654	0.6798096532970768
6	0.037064492216456635	0.20394289598912305
7	0.037064492216456635	0.2379333786539769
8	0.0	0.0
9	0.0	0.0
>10	0.14825796886582654	2.8891910265125764
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCAGG	30	1.0197144799456153	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACGCCGG	29	0.9857239972807614	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACCCGCCGG	14	0.4758667573079538	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	12	0.4078857919782461	No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGAGACACGCCGG	7	0.2379333786539769	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACACGCCGG	6	0.20394289598912305	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACACGCAGG	5	0.1699524133242692	No Hit
TTGGACGGGGTTTGTGTCTGCTTGTCTGTGTATACCACCT	5	0.1699524133242692	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	5	0.1699524133242692	No Hit
TTGGACGGGAAGCAGTGGTACGAGAGTCTTTTTTTTTTTT	5	0.1699524133242692	No Hit
TTGGACGGGCAGAGCTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.13596193065941536	No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGAGACACGCAGG	4	0.13596193065941536	No Hit
TTGGACGGGTACGCACAGCTCTGAGTCGGCGACACGCCGG	4	0.13596193065941536	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	4	0.13596193065941536	No Hit
TTGGACGGGTACGCACAGTTCTGCGTCGGAGACACGCAGG	4	0.13596193065941536	No Hit
TTGGACGGGTTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.13596193065941536	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	4	0.13596193065941536	No Hit
TTGGACGGGTACGCACCGTTCTGAGTCGGAGACACGCAGG	4	0.13596193065941536	No Hit
TTGGACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.13596193065941536	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	3	0.10197144799456152	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT	3	0.10197144799456152	No Hit
TTGGACGGGGTTTGTGTCTGCTTGTCTGTGTATACCCCCT	3	0.10197144799456152	No Hit
TTGGACGGGAAGCTGGTATCGATTTTTTTTTTTTTTTTTT	3	0.10197144799456152	No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10197144799456152	No Hit
TTGGACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGCGC	3	0.10197144799456152	No Hit
TTGGACGGGTACGCACCGTTCTGAGTCGGAGACACGCCGG	3	0.10197144799456152	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGCGACCCGCAGG	3	0.10197144799456152	No Hit
TTGGACGGGTACGCACCGTTCTGCGTCGGAGACACGCCGG	3	0.10197144799456152	No Hit
TTGGACGGGTACGCACAGTTCTGAGTCGGAGACACCCCGG	3	0.10197144799456152	No Hit
TTGGACGGGATTTTGTGGTAGTTTGAATTGTATCACTTCT	3	0.10197144799456152	No Hit
TTGGACGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	3	0.10197144799456152	No Hit
TTGGACGGGGTGATGGTGGTGGTGGTAGGGGTACGCTTAG	3	0.10197144799456152	No Hit
TTGGACGGGATGAAACACTGTCTCTTCTATAGGATCCTTT	3	0.10197144799456152	No Hit
TTGGACGGGTACGCCCAGTTCTGAGTCGGAGACACGCCGG	3	0.10197144799456152	No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.10197144799456152	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	3	0.10197144799456152	No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	3	0.10197144799456152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.03399048266485384	0.0	0.0	0.0	0.0
11	0.03399048266485384	0.0	0.0	0.0	0.0
12	0.03399048266485384	0.0	0.0	0.0	0.0
13	0.03399048266485384	0.0	0.0	0.0	0.0
14	0.03399048266485384	0.0	0.0	0.0	0.0
15	0.06798096532970768	0.0	0.0	0.0	0.0
16	0.06798096532970768	0.0	0.0	0.0	0.0
17	0.06798096532970768	0.0	0.0	0.0	0.0
18	0.06798096532970768	0.0	0.0	0.0	0.0
19	0.06798096532970768	0.0	0.0	0.0	0.0
20	0.06798096532970768	0.0	0.0	0.0	0.0
21	0.06798096532970768	0.0	0.0	0.0	0.0
22	0.06798096532970768	0.0	0.0	0.0	0.0
23	0.06798096532970768	0.0	0.0	0.0	0.0
24	0.06798096532970768	0.0	0.0	0.0	0.0
25	0.06798096532970768	0.0	0.0	0.0	0.0
26	0.06798096532970768	0.0	0.0	0.0	0.0
27	0.06798096532970768	0.0	0.0	0.0	0.0
28	0.06798096532970768	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGGT	55	9.094947E-12	34.473686	4
GACGGGG	95	0.0	34.473686	4
CGGGAAG	35	6.011669E-7	34.473686	6
TTGGACG	280	0.0	34.473686	1
CGGGGGG	30	9.378771E-6	34.473682	6
GGGTACG	30	9.378771E-6	34.473682	7
GACGGGC	20	0.0021954677	34.473682	4
GACGGGA	90	0.0	34.473682	4
CGGGTAC	40	3.7982318E-8	34.473682	6
ACGGGTA	45	2.3628672E-9	34.473682	5
ACGGGGG	45	2.3628672E-9	34.473682	5
ACGGGGA	30	9.378771E-6	34.473682	5
ACGGGAG	20	0.0021954677	34.473682	5
ACGGGAA	50	1.4370016E-10	34.473682	5
TGGACGG	265	0.0	34.473682	2
GGGAAGC	30	9.378771E-6	34.473682	7
GGACGGG	260	0.0	34.473682	3
ACACGCC	20	0.002398522	33.87931	32
CTGAGTC	30	1.0637619E-5	33.87931	21
ACGCCGG	20	0.002398522	33.87931	34
>>END_MODULE