FastQCFastQC Report
Fri 17 Jun 2016
SRR1525987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT180.8352668213457077No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC160.7424593967517401No Hit
TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT120.5568445475638051No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.41763341067285387No Hit
TTGGACGGGGCTGCAATTTTTGTGACTGAATAGGAAAAAA60.27842227378190254No Hit
TTGGACGGGCGGAATTGTTTTATCCAACTCATCCCTTGAC60.27842227378190254No Hit
TTGGACGGGGTTTTAAGTCCTTCCCTACTTGTCTCCCTTT60.27842227378190254No Hit
TTGGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC50.23201856148491878No Hit
TTGGACGGGGGGTTTCCCTGTGTTATCACCCTTCCAAGAA50.23201856148491878No Hit
TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG50.23201856148491878No Hit
TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA40.18561484918793503No Hit
TTGGACGGGGGACCAAATAGTTCTGACCCATTTCCCAACT40.18561484918793503No Hit
TTGGACGGGGGTCTATTGGAGTATGATAAAGAAAGGGCTT40.18561484918793503No Hit
TTGGACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG30.13921113689095127No Hit
TTGGACGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT30.13921113689095127No Hit
TTGGACGGGGTGCTTGGCTGGTTAATCTTTGGTAATTTCA30.13921113689095127No Hit
TTGGACGGGATCTCATGGATGTTTAATGATGTCCGTTCTG30.13921113689095127No Hit
TTGGACGGGAGGGGCTGGAGAGATGGCTCAGTGGTTAAGA30.13921113689095127No Hit
TTGGACGGGACTTGCCTAACTCTCAAACTGTTGGGGTGAT30.13921113689095127No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG30.13921113689095127No Hit
TTGGACGGGGGAAAATAAAGTGAAAGTTATAAATCTGGTG30.13921113689095127No Hit
TTGGACGGGATCTTGAGGCTGTAAATGATTCTGCCTCTCT30.13921113689095127No Hit
TTGGACGGGGAGCATGTCATCAAGAAGGAGATGTCTGGGG30.13921113689095127No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAG30.13921113689095127No Hit
TTGGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAC30.13921113689095127No Hit
TTGGACGGGATCGAATTATCTGAAGGGGAACTGGTCGCAG30.13921113689095127No Hit
TTGGACGGGATCAATGGGAGCAGTGTTTGCTATCATAGCA30.13921113689095127No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.13921113689095127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGG700.034.0000044
GACGGGC355.6526005E-734.0000044
CGGGATC309.095787E-634.0000046
ACGGGGG309.095787E-634.0000045
ACGGGAT355.6526005E-734.0000045
GACGGGA750.034.04
ACGGGGT251.433877E-434.05
ACGGGAG251.433877E-434.05
TGGACGG2050.034.02
TTGGACG2050.034.01
GGACGGG2000.034.03
GACGGGT200.002217195233.9999964
ACGGGCT200.002217195233.9999965
Produced by FastQC (version 0.11.5)