##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525987_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2155 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.62830626450116 29.0 4.0 30.0 4.0 33.0 2 12.85568445475638 4.0 4.0 24.0 4.0 30.0 3 11.20046403712297 4.0 4.0 19.0 4.0 27.0 4 10.768445475638051 4.0 4.0 17.0 4.0 27.0 5 11.567981438515082 4.0 4.0 19.0 4.0 30.0 6 11.877958236658932 4.0 4.0 20.0 4.0 30.0 7 12.670533642691415 4.0 4.0 24.0 4.0 32.0 8 12.572157772621809 4.0 4.0 24.0 4.0 32.0 9 12.308120649651972 4.0 4.0 23.0 4.0 32.0 10 11.444547563805104 4.0 4.0 20.0 4.0 30.0 11 12.309976798143852 4.0 4.0 24.0 4.0 33.0 12 11.922969837587006 4.0 4.0 23.0 4.0 32.0 13 10.94385150812065 4.0 4.0 18.0 4.0 31.0 14 11.678886310904872 4.0 4.0 22.0 4.0 33.0 15 11.278422273781903 4.0 4.0 20.0 4.0 33.0 16 9.2292343387471 4.0 4.0 9.0 4.0 27.0 17 8.750348027842227 4.0 4.0 7.0 4.0 26.0 18 8.643155452436195 4.0 4.0 4.0 4.0 26.0 19 8.392575406032483 4.0 4.0 4.0 4.0 26.0 20 8.572157772621809 4.0 4.0 4.0 4.0 26.0 21 7.500696055684455 4.0 4.0 4.0 4.0 22.0 22 7.432482598607889 4.0 4.0 4.0 4.0 22.0 23 7.40092807424594 4.0 4.0 4.0 4.0 21.0 24 7.191647331786543 4.0 4.0 4.0 4.0 20.0 25 7.348955916473318 4.0 4.0 4.0 4.0 21.0 26 7.54477958236659 4.0 4.0 4.0 4.0 23.0 27 7.5846867749419955 4.0 4.0 4.0 4.0 23.0 28 7.6682134570765665 4.0 4.0 4.0 4.0 24.0 29 7.163805104408353 4.0 4.0 4.0 4.0 19.0 30 7.4658932714617166 4.0 4.0 4.0 4.0 22.0 31 8.03062645011601 4.0 4.0 4.0 4.0 27.0 32 7.894199535962877 4.0 4.0 4.0 4.0 26.0 33 7.848259860788863 4.0 4.0 4.0 4.0 26.0 34 7.82830626450116 4.0 4.0 4.0 4.0 28.0 35 7.645939675174014 4.0 4.0 4.0 4.0 27.0 36 7.579582366589327 4.0 4.0 4.0 4.0 26.0 37 7.636658932714617 4.0 4.0 4.0 4.0 28.0 38 7.540139211136891 4.0 4.0 4.0 4.0 26.0 39 7.42877030162413 4.0 4.0 4.0 4.0 26.0 40 7.4218097447795826 4.0 4.0 4.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 2.674999999999997 2 2 -3.0630681818181813 2 3 1.1119318181818176 2 4 -1.7143939393939363 2 5 4.988257575757572 2 6 -0.5005681818181795 2 7 5.166856060606062 2 8 3.8941287878787882 2 9 -6.3022727272727295 2 10 0.12575757575757507 2 11 -4.518371212121211 2 12 4.919696969696968 2 13 4.232575757575757 2 14 -0.7787878787878793 2 15 5.244128787878786 2 16 0.41231060606060765 2 17 5.503598484848485 2 18 5.8797348484848495 2 19 6.926704545454546 2 20 2.0486742424242426 2 21 4.386931818181819 2 22 3.9785984848484848 2 23 7.6225378787878775 2 24 8.589204545454546 2 25 4.816287878787877 2 26 6.36628787878788 2 27 11.144886363636363 2 28 -0.5971590909090905 2 29 9.160416666666666 2 30 7.423674242424241 2 31 11.36534090909091 2 32 10.106439393939393 2 33 9.008522727272727 2 34 12.079166666666667 2 35 14.09810606060606 2 36 15.656060606060608 2 37 15.552083333333334 2 38 14.797348484848486 2 39 17.21212121212121 2 40 14.750946969696967 3 1 10.674999999999997 3 2 13.936931818181819 3 3 8.445265151515152 3 4 13.95227272727273 3 5 5.988257575757572 3 6 19.166098484848487 3 7 9.83352272727273 3 8 14.894128787878788 3 9 18.69772727272727 3 10 13.459090909090909 3 11 25.48162878787879 3 12 25.586363636363636 3 13 26.899242424242424 3 14 24.554545454545455 3 15 24.244128787878786 3 16 28.078977272727272 3 17 20.170265151515153 3 18 26.213068181818183 3 19 26.26003787878788 3 20 19.04867424242424 3 21 26.053598484848486 3 22 24.978598484848483 3 23 25.95587121212121 3 24 5.589204545454546 3 25 19.816287878787875 3 26 27.032954545454544 3 27 24.478219696969695 3 28 21.73617424242424 3 29 18.160416666666666 3 30 26.757007575757576 3 31 24.36534090909091 3 32 9.773106060606061 3 33 22.008522727272727 3 34 -3.5875000000000004 3 35 -3.90189393939394 3 36 -4.01060606060606 3 37 -4.114583333333334 3 38 -3.8693181818181817 3 39 -3.787878787878787 3 40 -3.582386363636365 4 1 -4.825000000000003 4 2 -7.229734848484848 4 3 -2.3880681818181824 4 4 -2.881060606060604 4 5 -5.345075757575761 4 6 -4.1672348484848465 4 7 -8.333143939393938 4 8 -4.605871212121212 4 9 -3.6356060606060634 4 10 -4.874242424242424 4 11 -4.685037878787878 4 12 -5.413636363636364 4 13 -3.6007575757575765 4 14 -6.945454545454545 4 15 -4.255871212121214 4 16 -4.587689393939393 4 17 -1.4964015151515158 4 18 -1.1202651515151496 4 19 -2.7399621212121197 4 20 -3.9513257575757574 4 21 0.720265151515151 4 22 -3.188068181818182 4 23 -2.5441287878787886 4 24 0.589204545454546 4 25 -2.683712121212123 4 26 -2.8003787878787874 4 27 -1.8551136363636376 4 28 -1.5971590909090905 4 29 0.49375000000000036 4 30 0.9236742424242408 4 31 -2.4679924242424223 4 32 0.6064393939393948 4 33 0.8418560606060597 4 34 -1.2541666666666673 4 35 -1.5685606060606068 4 36 0.9893939393939402 4 37 0.71875 4 38 0.9640151515151523 4 39 0.5454545454545467 4 40 1.084280303030301 6 1 16.174999999999997 6 2 20.18693181818182 6 3 13.445265151515152 6 4 8.20227272727273 6 5 22.238257575757572 6 6 6.4160984848484865 6 7 0.8335227272727295 6 8 4.894128787878788 6 9 6.4477272727272705 6 10 1.709090909090909 6 11 -2.0183712121212114 6 12 4.336363636363636 6 13 -5.1007575757575765 6 14 -0.4454545454545453 6 15 -0.5058712121212139 6 16 0.32897727272727373 6 17 -1.8297348484848488 6 18 -3.0369318181818166 6 19 -1.9899621212121197 6 20 -1.2013257575757574 6 21 -0.696401515151515 6 22 -0.5214015151515152 6 23 1.7058712121212114 6 24 -1.160795454545454 6 25 0.5662878787878771 6 26 -2.7170454545454543 6 27 -1.7717803030303045 6 28 -1.5138257575757574 6 29 -0.8395833333333336 6 30 0.7570075757575747 6 31 -0.13465909090908923 6 32 0.7731060606060609 6 33 0.7585227272727257 6 34 0.16249999999999964 6 35 1.8481060606060602 6 36 4.48939393939394 6 37 3.135416666666666 6 38 4.630681818181818 6 39 3.462121212121213 6 40 3.667613636363635 7 1 1.6749999999999972 7 2 5.936931818181819 7 3 0.44526515151515156 7 4 0.28560606060606375 7 5 -4.678409090909095 7 6 -0.5005681818181795 7 7 -5.1664772727272705 7 8 -0.4392045454545457 7 9 -2.9689393939393955 7 10 -2.207575757575757 7 11 -7.185037878787878 7 12 -4.746969696969697 7 13 0.23257575757575744 7 14 -6.112121212121212 7 15 -5.755871212121214 7 16 2.0789772727272737 7 17 -2.829734848484849 7 18 2.5464015151515174 7 19 3.2600378787878803 7 20 -2.9513257575757574 7 21 7.053598484848485 7 22 6.311931818181819 7 23 5.6225378787878775 7 24 1.255871212121212 7 25 6.816287878787877 7 26 -4.967045454545454 7 27 -4.5217803030303045 7 28 -4.263825757575757 7 29 -3.8395833333333336 7 30 -4.242992424242425 7 31 -4.634659090909089 7 32 -4.226893939393939 7 33 -3.9914772727272743 7 34 -3.5875000000000004 7 35 -3.90189393939394 7 36 -4.01060606060606 7 37 -4.114583333333334 7 38 -3.8693181818181817 7 39 -3.787878787878787 7 40 -3.582386363636365 8 1 1.8749999999999964 8 2 -9.063068181818181 8 3 -7.554734848484848 8 4 -7.04772727272727 8 5 -7.011742424242428 8 6 -7.8339015151515135 8 7 -9.16647727272727 8 8 -9.105871212121212 8 9 -8.30227272727273 8 10 -7.540909090909091 8 11 -8.518371212121211 8 12 -8.413636363636364 8 13 -7.1007575757575765 8 14 -7.445454545454545 8 15 -7.755871212121214 8 16 -5.921022727272726 8 17 -4.829734848484849 8 18 -4.786931818181817 8 19 -4.73996212121212 8 20 -4.951325757575757 8 21 -3.946401515151515 8 22 -4.021401515151515 8 23 -4.044128787878789 8 24 -3.410795454545454 8 25 -4.183712121212123 8 26 -4.967045454545454 8 27 -4.5217803030303045 8 28 -4.263825757575757 8 29 -3.8395833333333336 8 30 -4.242992424242425 8 31 -4.634659090909089 8 32 -4.226893939393939 8 33 -3.9914772727272743 8 34 -3.5875000000000004 8 35 -3.90189393939394 8 36 -4.01060606060606 8 37 -4.114583333333334 8 38 -3.8693181818181817 8 39 -3.787878787878787 8 40 -3.582386363636365 9 1 12.341666666666665 9 2 14.27026515151515 9 3 13.778598484848484 9 4 14.618939393939398 9 5 0.32159090909090615 9 6 13.499431818181819 9 7 17.83352272727273 9 8 0.5607954545454543 9 9 7.031060606060603 9 10 1.792424242424243 9 11 3.4816287878787886 9 12 15.253030303030304 9 13 6.565909090909091 9 14 14.887878787878787 9 15 13.910795454545454 9 16 23.745643939393936 9 17 11.170265151515151 9 18 5.213068181818183 9 19 0.9267045454545464 9 20 5.715340909090909 9 21 1.720265151515151 9 22 7.645265151515151 9 23 5.955871212121211 9 24 11.589204545454546 9 25 4.149621212121211 9 26 15.366287878787878 9 27 8.144886363636363 9 28 9.40284090909091 9 29 10.160416666666666 9 30 14.090340909090907 9 31 7.032007575757577 9 32 12.773106060606061 9 33 9.008522727272727 9 34 9.4125 9 35 7.764772727272726 9 36 13.98939393939394 9 37 13.552083333333334 9 38 3.7973484848484844 9 39 9.545454545454545 9 40 14.084280303030303 10 1 15.674999999999997 10 2 15.936931818181819 10 3 18.44526515151515 10 4 10.95227272727273 10 5 20.988257575757572 10 6 21.166098484848487 10 7 19.83352272727273 10 8 19.89412878787879 10 9 22.69772727272727 10 10 14.459090909090909 10 11 20.48162878787879 10 12 20.586363636363636 10 13 6.8992424242424235 10 14 10.554545454545455 10 15 21.244128787878786 10 16 16.078977272727272 10 17 7.170265151515151 10 18 8.213068181818183 10 19 2.2600378787878803 10 20 20.04867424242424 10 21 4.053598484848485 10 22 14.978598484848485 10 23 8.955871212121211 10 24 4.589204545454546 10 25 21.816287878787875 10 26 22.032954545454544 10 27 12.478219696969695 10 28 22.73617424242424 10 29 6.160416666666666 10 30 3.7570075757575747 10 31 11.36534090909091 10 32 13.773106060606061 10 33 18.008522727272727 10 34 21.4125 10 35 18.09810606060606 10 36 20.989393939393942 10 37 22.885416666666664 10 38 23.13068181818182 10 39 7.212121212121213 10 40 18.417613636363633 11 1 10.674999999999997 11 2 15.936931818181819 11 3 4.445265151515152 11 4 9.95227272727273 11 5 1.9882575757575722 11 6 15.166098484848487 11 7 16.83352272727273 11 8 8.894128787878788 11 9 11.69772727272727 11 10 10.459090909090909 11 11 21.48162878787879 11 12 15.586363636363636 11 13 18.899242424242424 11 14 16.554545454545455 11 15 18.244128787878786 11 16 -5.921022727272726 11 17 -4.829734848484849 11 18 -4.786931818181817 11 19 -4.73996212121212 11 20 -4.951325757575757 11 21 -3.946401515151515 11 22 -4.021401515151515 11 23 -4.044128787878789 11 24 -3.410795454545454 11 25 -4.183712121212123 11 26 -4.967045454545454 11 27 -4.5217803030303045 11 28 -4.263825757575757 11 29 -3.8395833333333336 11 30 -4.242992424242425 11 31 -4.634659090909089 11 32 -4.226893939393939 11 33 -3.9914772727272743 11 34 -3.5875000000000004 11 35 -3.90189393939394 11 36 -4.01060606060606 11 37 -4.114583333333334 11 38 -3.8693181818181817 11 39 -3.787878787878787 11 40 -3.582386363636365 12 1 16.674999999999997 12 2 21.93693181818182 12 3 16.64526515151515 12 4 11.75227272727273 12 5 18.588257575757574 12 6 23.166098484848487 12 7 21.033522727272732 12 8 18.69412878787879 12 9 22.09772727272727 12 10 16.259090909090908 12 11 7.881628787878787 12 12 10.186363636363637 12 13 7.299242424242422 12 14 9.554545454545455 12 15 5.444128787878785 12 16 5.878977272727274 12 17 5.3702651515151505 12 18 5.013068181818184 12 19 6.060037878787881 12 20 5.448674242424243 12 21 6.653598484848485 12 22 6.7785984848484855 12 23 7.755871212121212 12 24 10.589204545454546 12 25 7.416287878787877 12 26 12.432954545454544 12 27 3.878219696969696 12 28 7.136174242424243 12 29 7.160416666666666 12 30 5.757007575757575 12 31 6.96534090909091 12 32 7.773106060606061 12 33 7.208522727272725 12 34 8.812499999999998 12 35 8.29810606060606 12 36 8.18939393939394 12 37 8.085416666666665 12 38 7.530681818181819 12 39 4.612121212121213 12 40 9.217613636363636 13 1 -1.3250000000000028 13 2 -3.3130681818181813 13 3 -4.054734848484848 13 4 -5.54772727272727 13 5 -0.5117424242424278 13 6 -0.5839015151515135 13 7 -3.1664772727272705 13 8 -3.1058712121212118 13 9 -1.0522727272727295 13 10 -0.290909090909091 13 11 -8.518371212121211 13 12 -8.413636363636364 13 13 -7.1007575757575765 13 14 -7.445454545454545 13 15 -7.755871212121214 13 16 -5.921022727272726 13 17 -4.829734848484849 13 18 -4.786931818181817 13 19 -4.73996212121212 13 20 -4.951325757575757 13 21 -3.946401515151515 13 22 -4.021401515151515 13 23 -4.044128787878789 13 24 -3.410795454545454 13 25 -4.183712121212123 13 26 -4.967045454545454 13 27 -4.5217803030303045 13 28 -4.263825757575757 13 29 -3.8395833333333336 13 30 -4.242992424242425 13 31 -4.634659090909089 13 32 -4.226893939393939 13 33 -3.9914772727272743 13 34 -3.5875000000000004 13 35 -3.90189393939394 13 36 -4.01060606060606 13 37 -4.114583333333334 13 38 -3.8693181818181817 13 39 -3.787878787878787 13 40 -3.582386363636365 15 1 17.074999999999996 15 2 17.93693181818182 15 3 17.045265151515153 15 4 21.352272727272727 15 5 19.18825757575757 15 6 19.966098484848487 15 7 16.63352272727273 15 8 19.89412878787879 15 9 20.69772727272727 15 10 17.059090909090912 15 11 15.481628787878789 15 12 14.386363636363637 15 13 6.8992424242424235 15 14 7.554545454545455 15 15 14.644128787878785 15 16 6.278977272727273 15 17 8.770265151515153 15 18 8.013068181818184 15 19 6.46003787878788 15 20 6.248674242424242 15 21 11.853598484848487 15 22 7.978598484848485 15 23 9.755871212121212 15 24 8.789204545454545 15 25 7.016287878787876 15 26 6.632954545454545 15 27 10.678219696969695 15 28 10.336174242424244 15 29 3.560416666666667 15 30 14.757007575757575 15 31 14.36534090909091 15 32 15.573106060606062 15 33 12.208522727272726 15 34 13.012500000000001 15 35 16.09810606060606 15 36 14.589393939393942 15 37 17.885416666666664 15 38 17.53068181818182 15 39 13.012121212121214 15 40 15.417613636363635 16 1 1.6749999999999972 16 2 4.270265151515151 16 3 3.1119318181818176 16 4 3.95227272727273 16 5 -2.678409090909094 16 6 -4.8339015151515135 16 7 6.8335227272727295 16 8 6.894128787878788 16 9 5.6977272727272705 16 10 5.459090909090909 16 11 8.81496212121212 16 12 5.919696969696968 16 13 1.2325757575757574 16 14 10.221212121212123 16 15 4.244128787878786 16 16 10.412310606060606 16 17 10.170265151515151 16 18 5.8797348484848495 16 19 7.5933712121212125 16 20 11.048674242424243 16 21 7.720265151515151 16 22 2.3119318181818187 16 23 1.2892045454545453 16 24 10.922537878787878 16 25 8.816287878787877 16 26 6.36628787878788 16 27 9.478219696969695 16 28 12.069507575757575 16 29 7.49375 16 30 8.423674242424243 16 31 5.365340909090911 16 32 7.439772727272727 16 33 7.008522727272726 16 34 7.079166666666666 16 35 10.431439393939392 16 36 13.322727272727272 16 37 13.552083333333334 16 38 10.130681818181818 16 39 11.545454545454545 16 40 12.750946969696967 17 1 16.34166666666666 17 2 15.27026515151515 17 3 15.778598484848484 17 4 17.6189393939394 17 5 19.65492424242424 17 6 24.499431818181822 17 7 22.500189393939394 17 8 22.89412878787879 17 9 23.69772727272727 17 10 20.792424242424246 17 11 23.48162878787879 17 12 18.919696969696968 17 13 19.232575757575756 17 14 20.88787878787879 17 15 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13.170265151515151 117 18 -1.7869318181818166 117 19 13.26003787878788 117 20 16.04867424242424 117 21 6.053598484848485 117 22 21.978598484848483 117 23 13.955871212121211 117 24 21.589204545454546 117 25 -1.1837121212121229 117 26 22.032954545454544 117 27 20.478219696969695 117 28 19.73617424242424 117 29 9.160416666666666 117 30 22.757007575757576 117 31 21.36534090909091 117 32 -4.226893939393939 117 33 -3.9914772727272743 117 34 -3.5875000000000004 117 35 -3.90189393939394 117 36 -4.01060606060606 117 37 -4.114583333333334 117 38 -3.8693181818181817 117 39 -3.787878787878787 117 40 -3.582386363636365 120 1 11.674999999999997 120 2 12.936931818181819 120 3 0.44526515151515156 120 4 7.95227272727273 120 5 8.988257575757572 120 6 14.166098484848487 120 7 16.83352272727273 120 8 16.89412878787879 120 9 -8.30227272727273 120 10 -7.540909090909091 120 11 -8.518371212121211 120 12 -8.413636363636364 120 13 -7.1007575757575765 120 14 -7.445454545454545 120 15 -7.755871212121214 120 16 -5.921022727272726 120 17 -4.829734848484849 120 18 -4.786931818181817 120 19 -4.73996212121212 120 20 -4.951325757575757 120 21 -3.946401515151515 120 22 -4.021401515151515 120 23 -4.044128787878789 120 24 -3.410795454545454 120 25 -4.183712121212123 120 26 -4.967045454545454 120 27 -4.5217803030303045 120 28 -4.263825757575757 120 29 -3.8395833333333336 120 30 -4.242992424242425 120 31 -4.634659090909089 120 32 -4.226893939393939 120 33 -3.9914772727272743 120 34 -3.5875000000000004 120 35 -3.90189393939394 120 36 -4.01060606060606 120 37 -4.114583333333334 120 38 -3.8693181818181817 120 39 -3.787878787878787 120 40 -3.582386363636365 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1198.0 5 47.0 6 34.0 7 39.0 8 27.0 9 58.0 10 82.0 11 55.0 12 65.0 13 50.0 14 46.0 15 19.0 16 23.0 17 14.0 18 19.0 19 17.0 20 50.0 21 59.0 22 48.0 23 44.0 24 38.0 25 36.0 26 29.0 27 20.0 28 19.0 29 9.0 30 7.0 31 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.07192575406032 0.6496519721577726 0.09280742459396751 0.18561484918793503 8 98.65366759517178 0.3714020427112349 0.5571030640668524 0.4178272980501393 9 98.51370181142592 0.603808639108221 0.41802136553646074 0.46446818392940087 10 50.81206496519721 27.98143851508121 8.631090487238978 12.575406032482597 11 35.45243619489559 21.48491879350348 27.00696055684455 16.05568445475638 12 32.25058004640371 19.16473317865429 25.89327146171694 22.691415313225058 13 23.7122969837587 20.0 29.976798143851507 26.31090487238979 14 21.995359628770302 26.264501160092806 30.580046403712295 21.160092807424594 15 27.703016241299306 25.150812064965194 29.883990719257543 17.262180974477957 16 32.99303944315545 27.424593967517403 24.269141531322504 15.31322505800464 17 27.762302692664807 25.20891364902507 29.99071494893222 17.038068709377903 18 26.264501160092806 25.661252900232018 29.4199535962877 18.65429234338747 19 26.648096564531105 29.247910863509752 25.487465181058493 18.61652739090065 20 27.656612529002324 25.93967517401392 31.044083526682137 15.359628770301626 21 23.34106728538283 26.17169373549884 31.69373549883991 18.793503480278424 22 22.96983758700696 24.037122969837586 32.11136890951276 20.88167053364269 23 21.160092807424594 27.285382830626446 32.34338747099768 19.211136890951273 24 23.201856148491878 25.93967517401392 30.162412993039446 20.696055684454755 25 23.130515559684163 30.00464468183929 26.428239665582907 20.436600092893638 26 24.417520969245107 28.704566635601118 26.37465051258155 20.503261882572225 27 19.93479273404751 27.619934792734046 27.666511411271543 24.778761061946902 28 21.634168987929435 29.99071494893222 25.81244196843083 22.56267409470752 29 25.41899441340782 28.35195530726257 25.512104283054004 20.716945996275605 30 24.37325905292479 26.648096564531105 29.805013927576603 19.1736304549675 31 26.589327146171694 28.02784222737819 26.450116009280745 18.932714617169374 32 25.896599906846763 29.250116441546343 28.085700978108992 16.767582673497905 33 26.450116009280745 26.728538283062647 27.842227378190255 18.979118329466356 34 22.366589327146173 28.491879350348025 30.533642691415313 18.60788863109049 35 20.742459396751737 29.698375870069604 31.740139211136892 17.819025522041763 36 20.810055865921786 30.679702048417134 29.05027932960894 19.45996275605214 37 24.593967517401392 28.445475638051043 29.280742459396752 17.679814385150813 38 24.872389791183295 29.791183294663576 26.54292343387471 18.793503480278424 39 24.17633410672854 29.280742459396752 26.032482598607885 20.51044083526682 40 22.59860788863109 26.032482598607885 29.23433874709977 22.134570765661252 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 3.5 17 5.0 18 5.0 19 6.0 20 7.0 21 11.0 22 15.0 23 15.0 24 12.0 25 9.0 26 15.5 27 22.0 28 22.0 29 25.0 30 28.0 31 43.0 32 58.0 33 58.0 34 57.5 35 57.0 36 77.0 37 97.0 38 97.0 39 106.5 40 116.0 41 120.0 42 124.0 43 124.0 44 157.0 45 190.0 46 194.5 47 199.0 48 199.0 49 196.0 50 193.0 51 211.5 52 230.0 53 230.0 54 201.5 55 173.0 56 173.5 57 174.0 58 174.0 59 178.0 60 182.0 61 167.5 62 153.0 63 153.0 64 106.5 65 60.0 66 47.0 67 34.0 68 34.0 69 24.0 70 14.0 71 10.0 72 6.0 73 6.0 74 4.5 75 3.0 76 3.0 77 3.0 78 3.0 79 2.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.04640371229698376 9 0.09280742459396751 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.04640371229698376 18 0.0 19 0.04640371229698376 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.09280742459396751 26 0.41763341067285387 27 0.37122969837587005 28 0.04640371229698376 29 0.3248259860788863 30 0.04640371229698376 31 0.0 32 0.37122969837587005 33 0.0 34 0.0 35 0.0 36 0.3248259860788863 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2155.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.81206496519721 #Duplication Level Percentage of deduplicated Percentage of total 1 94.43025038323965 85.75406032482599 2 4.1389882473173225 7.517401392111369 3 0.7664793050587635 2.088167053364269 4 0.1532958610117527 0.5568445475638051 5 0.1532958610117527 0.6960556844547563 6 0.1532958610117527 0.8352668213457077 7 0.0 0.0 8 0.0 0.0 9 0.0510986203372509 0.41763341067285387 >10 0.1532958610117527 2.134570765661253 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 18 0.8352668213457077 No Hit TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 16 0.7424593967517401 No Hit TTGGACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 12 0.5568445475638051 No Hit TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 9 0.41763341067285387 No Hit TTGGACGGGGCTGCAATTTTTGTGACTGAATAGGAAAAAA 6 0.27842227378190254 No Hit TTGGACGGGCGGAATTGTTTTATCCAACTCATCCCTTGAC 6 0.27842227378190254 No Hit TTGGACGGGGTTTTAAGTCCTTCCCTACTTGTCTCCCTTT 6 0.27842227378190254 No Hit TTGGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 5 0.23201856148491878 No Hit TTGGACGGGGGGTTTCCCTGTGTTATCACCCTTCCAAGAA 5 0.23201856148491878 No Hit TTGGACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 5 0.23201856148491878 No Hit TTGGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 4 0.18561484918793503 No Hit TTGGACGGGGGACCAAATAGTTCTGACCCATTTCCCAACT 4 0.18561484918793503 No Hit TTGGACGGGGGTCTATTGGAGTATGATAAAGAAAGGGCTT 4 0.18561484918793503 No Hit TTGGACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 3 0.13921113689095127 No Hit TTGGACGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.13921113689095127 No Hit TTGGACGGGGTGCTTGGCTGGTTAATCTTTGGTAATTTCA 3 0.13921113689095127 No Hit TTGGACGGGATCTCATGGATGTTTAATGATGTCCGTTCTG 3 0.13921113689095127 No Hit TTGGACGGGAGGGGCTGGAGAGATGGCTCAGTGGTTAAGA 3 0.13921113689095127 No Hit TTGGACGGGACTTGCCTAACTCTCAAACTGTTGGGGTGAT 3 0.13921113689095127 No Hit TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG 3 0.13921113689095127 No Hit TTGGACGGGGGAAAATAAAGTGAAAGTTATAAATCTGGTG 3 0.13921113689095127 No Hit TTGGACGGGATCTTGAGGCTGTAAATGATTCTGCCTCTCT 3 0.13921113689095127 No Hit TTGGACGGGGAGCATGTCATCAAGAAGGAGATGTCTGGGG 3 0.13921113689095127 No Hit TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAG 3 0.13921113689095127 No Hit TTGGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAC 3 0.13921113689095127 No Hit TTGGACGGGATCGAATTATCTGAAGGGGAACTGGTCGCAG 3 0.13921113689095127 No Hit TTGGACGGGATCAATGGGAGCAGTGTTTGCTATCATAGCA 3 0.13921113689095127 No Hit TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.13921113689095127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGGG 70 0.0 34.000004 4 GACGGGC 35 5.6526005E-7 34.000004 4 CGGGATC 30 9.095787E-6 34.000004 6 ACGGGGG 30 9.095787E-6 34.000004 5 ACGGGAT 35 5.6526005E-7 34.000004 5 GACGGGA 75 0.0 34.0 4 ACGGGGT 25 1.433877E-4 34.0 5 ACGGGAG 25 1.433877E-4 34.0 5 TGGACGG 205 0.0 34.0 2 TTGGACG 205 0.0 34.0 1 GGACGGG 200 0.0 34.0 3 GACGGGT 20 0.0022171952 33.999996 4 ACGGGCT 20 0.0022171952 33.999996 5 >>END_MODULE