SRR1525986_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66643
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1153	1.730114190537641	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	945	1.4180033912038774	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	839	1.2589469261587862	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	309	0.4636646009333313	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	276	0.41414702219287847	No Hit
TCACTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	239	0.3586273126960071	No Hit
TCACTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	209	0.3136113320228681	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	187	0.2805996128625662	No Hit
TCACTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	151	0.22658043605479944	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	150	0.22507990336569483	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	146	0.21907777260927627	No Hit
TCACTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	142	0.2130756418528578	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	131	0.1965697822727068	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	131	0.1965697822727068	No Hit
TCACTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	128	0.19206818420539293	No Hit
TCACTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	124	0.18606605344897437	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	115	0.1725612592470327	No Hit
TCACTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	110	0.16505859580150953	No Hit
TCACTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	98	0.14705220353225396	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	95	0.14255060546494006	No Hit
TCACTTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	95	0.14255060546494006	No Hit
TCACTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	94	0.14105007277583542	No Hit
TCACTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	90	0.1350479420194169	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	87	0.130546343952103	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	86	0.12904581126299836	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	81	0.1215431478174752	No Hit
TCACTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	77	0.11554101706105668	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	74	0.11103941899374278	No Hit
TCACTTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	72	0.1080383536155335	No Hit
TCACTTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	70	0.10503728823732425	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCA	35	4.894173E-8	50.3713	41
CCCCGAC	40	2.4501787E-9	50.3713	41
TCCTAGA	15	0.008178537	50.333084	40
TCTAATC	15	0.008178537	50.333084	40
GTCATGG	15	0.008203118	50.29492	39
CCGATTG	35	4.95329E-8	50.29492	39
AGCCCTG	15	0.008203118	50.29492	38
TTGCTCA	20	4.036212E-4	50.29492	37
GTCTAAT	20	4.036212E-4	50.29492	39
GCCGATT	35	4.95329E-8	50.29492	38
AGCGCTT	15	0.008203118	50.29492	37
TGCTCAA	20	4.036212E-4	50.29492	38
TTGCATT	15	0.008203118	50.29492	39
GGTAATC	20	4.0665444E-4	50.218777	35
GTAATCC	15	0.008252447	50.218777	36
GAGCGCT	15	0.008252447	50.218777	36
CACTGTT	15	0.008326852	50.104984	43
TGCCTAG	15	0.008326852	50.104984	43
TCTCCAC	20	4.1123823E-4	50.104984	43
TAATCCG	15	0.008326852	50.104984	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content