##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66643
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.70654082199181	39.0	38.0	40.0	35.0	40.0
2	37.25663610581756	39.0	38.0	40.0	33.0	40.0
3	35.51412751526792	39.0	36.0	40.0	22.0	40.0
4	37.03667301892172	39.0	38.0	40.0	32.0	40.0
5	37.24203592275258	39.0	38.0	40.0	33.0	40.0
6	37.25564575424276	39.0	38.0	40.0	33.0	40.0
7	37.274012274357396	39.0	38.0	40.0	33.0	40.0
8	37.000225079903366	39.0	38.0	40.0	33.0	40.0
9	36.98376423630389	39.0	38.0	40.0	33.0	40.0
10	36.17370166409075	39.0	37.0	40.0	29.0	40.0
11	35.41362183575169	38.0	35.0	40.0	27.0	40.0
12	34.369041009558394	38.0	33.0	40.0	22.0	40.0
13	33.9014600183065	38.0	33.0	40.0	20.0	40.0
14	34.29809582401753	38.0	33.0	40.0	22.0	40.0
15	33.07727743348889	38.0	31.0	39.0	17.0	40.0
16	32.65568776915805	37.0	30.0	39.0	18.0	40.0
17	31.851777380970244	36.0	28.0	39.0	15.0	40.0
18	31.35436579985895	36.0	27.0	39.0	15.0	40.0
19	32.445208048857346	37.0	29.0	39.0	18.0	40.0
20	33.072655792806444	37.0	30.0	39.0	21.0	40.0
21	33.3859520129646	38.0	31.0	39.0	19.0	40.0
22	32.69704245006977	38.0	30.0	39.0	17.0	40.0
23	33.329051813393754	38.0	31.0	39.0	19.0	40.0
24	32.70709601908678	38.0	30.0	39.0	17.0	40.0
25	33.152754227750854	38.0	31.0	39.0	19.0	40.0
26	33.50521435109464	38.0	33.0	39.0	19.0	40.0
27	33.8417988385877	38.0	33.0	39.0	22.0	40.0
28	33.80059421094489	38.0	33.0	39.0	22.0	40.0
29	33.248038053509	38.0	32.0	39.0	18.0	40.0
30	33.165418723646894	38.0	32.0	39.0	18.0	40.0
31	33.8917365664811	38.0	33.0	39.0	22.0	40.0
32	33.809297300541694	38.0	33.0	39.0	21.0	40.0
33	33.58696337199706	38.0	33.0	39.0	20.0	40.0
34	33.50609966538121	38.0	33.0	39.0	19.0	40.0
35	33.5305883588674	38.0	33.0	39.0	19.0	40.0
36	32.769488168299745	37.0	32.0	39.0	17.0	40.0
37	32.49952733220293	37.0	32.0	39.0	14.0	40.0
38	31.784358447248774	36.0	30.0	39.0	9.0	40.0
39	30.65151628828234	36.0	28.0	38.0	4.0	40.0
40	28.594826163287966	35.0	22.0	38.0	4.0	40.0
41	26.361073180979247	31.0	15.0	38.0	4.0	40.0
42	27.38191558003091	32.0	20.0	38.0	4.0	39.0
43	27.703194634095105	33.0	21.0	38.0	4.0	40.0
44	26.604264513902436	32.0	17.0	38.0	4.0	40.0
45	27.58097624656753	33.0	21.0	38.0	4.0	40.0
46	25.569302102246297	30.0	16.0	36.0	4.0	39.0
47	24.6252719715499	29.0	10.0	36.0	4.0	39.0
48	25.246027339705595	30.0	15.0	36.0	4.0	39.0
49	24.266929760064823	29.0	10.0	36.0	4.0	39.0
50	25.03139114385607	30.0	14.0	36.0	4.0	39.0
51	25.302522395450385	31.0	9.0	37.0	4.0	39.0
52	25.15156880692646	31.0	4.0	37.0	4.0	39.0
53	25.028330057170294	31.0	4.0	37.0	4.0	39.0
54	24.639572048077067	30.0	4.0	36.0	4.0	39.0
55	24.482781387392524	30.0	4.0	36.0	4.0	39.0
56	23.618234473238	30.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.8589305649192909
1	2	2.24332699143563
1	3	4.842729430635572
1	4	2.5579323407876373
1	5	2.495685335603632
1	6	2.4429328141051982
1	7	2.405060714877635
1	8	2.708895220673
1	9	2.772028050992674
1	10	3.275567557343315
1	11	0.9204512667783149
1	12	-6.466849141846598
1	13	-19.07346275365864
1	14	-5.671922295997092
1	15	1.2233034507246892
1	16	-0.4266087707907147
1	17	-6.273127440846441
1	18	-3.970207579671378
1	19	0.742640692169946
1	20	-0.2523957246682329
1	21	5.583889368780472
1	22	6.66543283760527
1	23	5.95416795429459
1	24	6.331603366204579
1	25	5.984291318863583
1	26	5.406733554900637
1	27	4.9192754225348985
1	28	5.742108769581677
1	29	5.915139528110135
1	30	6.281389005874171
1	31	4.9661979976799095
1	32	4.814637450372015
1	33	5.313903675002095
1	34	4.920869541819787
1	35	3.3601719954066027
1	36	3.262559867652822
1	37	3.1852181406538485
1	38	4.32750380439526
1	39	5.249801097788662
1	40	-10.08958705927662
1	41	0.8552500122172333
1	42	-0.30655561499969863
1	43	-8.994068707729678
1	44	-15.34350450857122
1	45	-2.3212385785142615
1	46	-4.630301632680084
1	47	-9.98880322326872
1	48	-4.088248756123328
1	49	-13.658178502833701
1	50	-3.412335359381032
1	51	2.8775339002351785
1	52	2.7050256144127616
1	53	2.7710656453063365
1	54	3.6382597269433816
1	55	2.895017818219255
1	56	7.136635395730014
2	1	2.0078968634183028
2	2	2.6485946210023243
2	3	4.569341344163377
2	4	2.9126949198492795
2	5	2.786065775516782
2	6	2.683563419221784
2	7	2.8262822762861077
2	8	3.0396797009901917
2	9	3.074019932423802
2	10	3.616358645797959
2	11	3.5804153940321584
2	12	0.15384376856594173
2	13	1.5805540498908783
2	14	4.459674041206725
2	15	5.472524634525318
2	16	5.221555111018031
2	17	7.394011235638029
2	18	5.5337101018164
2	19	6.294042561328826
2	20	5.736181637740906
2	21	6.538104416453461
2	22	7.157739077575062
2	23	6.657934592640672
2	24	7.631329600510252
2	25	6.674841682311147
2	26	7.0027875528237935
2	27	6.280929344936485
2	28	6.102441064493412
2	29	6.428403003990436
2	30	6.296115712189664
2	31	5.231656319212998
2	32	5.510946333596788
2	33	5.292946439091587
2	34	5.485298881950062
2	35	5.290692150225823
2	36	6.415868656475634
2	37	6.708205018780912
2	38	7.740134787119697
2	39	8.454464554788565
2	40	6.31209329567476
2	41	10.666729290986229
2	42	9.263915450798468
2	43	8.667972262722504
2	44	-2.787854551052103
2	45	8.682159892173928
2	46	7.459191428775593
2	47	4.203210370614034
2	48	8.141242417293078
2	49	0.3353077616805997
2	50	7.944032053061147
2	51	10.093903200716628
2	52	10.010699172422765
2	53	10.277532179810255
2	54	10.416131906684722
2	55	10.332948527178
2	56	11.14399874888776
3	1	1.651813522939861
3	2	2.5410751148718305
3	3	4.534787627280927
3	4	2.8418726614252066
3	5	2.7510833524695357
3	6	2.5937066070808257
3	7	2.495321117064641
3	8	2.747816543997992
3	9	2.984334602748767
3	10	3.3313547874424714
3	11	3.865590734853562
3	12	4.4392763330862195
3	13	5.527223002990418
3	14	5.384050273736946
3	15	1.3823248574525202
3	16	6.455885900694785
3	17	7.70833859566547
3	18	7.771984988209265
3	19	6.687594820610311
3	20	6.0340466810402305
3	21	6.874038567186549
3	22	7.533285677935172
3	23	6.04479903575136
3	24	7.2567261537126875
3	25	6.563292338231577
3	26	6.08767137345313
3	27	5.1130480108028955
3	28	5.83792936081511
3	29	6.143570628100871
3	30	6.042578886330109
3	31	5.227712222496887
3	32	5.347952249741862
3	33	4.8799309199284195
3	34	4.858873433952116
3	35	4.687759800058629
3	36	6.40480803742393
3	37	6.258149286979492
3	38	6.923278060719969
3	39	8.418367495712857
3	40	9.731282183610968
3	41	12.38309729235809
3	42	10.56546736711503
3	43	10.499490739958208
3	44	10.829139361320355
3	45	10.481096700885239
3	46	11.49786072484007
3	47	11.6642409802342
3	48	11.447938807587171
3	49	12.129700285045086
3	50	10.84834483707898
3	51	10.8219320097709
3	52	10.717721379402104
3	53	10.780147287415499
3	54	10.959731323644338
3	55	10.806240133111292
3	56	10.902551436875413
4	1	1.9346881406768688
4	2	2.847605066301945
4	3	4.980281421128744
4	4	2.6803328161292868
4	5	2.683445288069464
4	6	2.884406373225822
4	7	2.808506941853274
4	8	3.0056866645232674
4	9	2.9508753474870275
4	10	3.4169816987574606
4	11	4.78913813546518
4	12	5.5726636329602925
4	13	6.340678660482773
4	14	5.64210028273137
4	15	6.340950509548218
4	16	7.718073344479638
4	17	8.701322945368656
4	18	9.019691410227612
4	19	7.870091672562101
4	20	6.8521794387304595
4	21	6.93475092552438
4	22	7.311303307005151
4	23	6.232242820604753
4	24	7.470343710827276
4	25	7.026774978994304
4	26	6.501504796730703
4	27	6.151004477793368
4	28	5.8042002728496485
4	29	6.512881655264028
4	30	6.360711644020338
4	31	4.977487361910452
4	32	4.686592292915098
4	33	4.950267311365728
4	34	4.100013926846525
4	35	5.6955849484666174
4	36	5.74443746717748
4	37	5.788010772858247
4	38	6.348894178726805
4	39	6.751880717515341
4	40	-0.20485740984108958
4	41	9.757804973595722
4	42	9.002416458678258
4	43	9.113180252460456
4	44	10.93635288893324
4	45	9.537941457136363
4	46	11.174511201544515
4	47	12.015653104717021
4	48	11.895411374595625
4	49	11.590484598770576
4	50	12.542804272229187
4	51	10.938139960841237
4	52	10.239487884169147
4	53	11.042604563904082
4	54	10.86582954274849
4	55	10.892046932895429
4	56	10.700806518724665
5	1	2.172061878050606
5	2	2.576660324768966
5	3	4.681113269019413
5	4	2.5579323407876373
5	5	2.707806547724843
5	6	2.7863671575395443
5	7	2.4757677855847007
5	8	2.6684911802689584
5	9	2.9538462328108537
5	10	3.5179917997675574
5	11	4.445703792030841
5	12	5.482645807648353
5	13	5.744719064523181
5	14	5.792724168649372
5	15	6.748555975977215
5	16	7.846118501936562
5	17	8.100609932890936
5	18	9.312620703156906
5	19	7.823448772978029
5	20	6.899119426846916
5	21	7.048535833426936
5	22	7.806846979019408
5	23	6.550127550254189
5	24	7.796249830851043
5	25	7.125705460277729
5	26	6.022895171062249
5	27	5.666750170009646
5	28	6.115846143319047
5	29	6.703018315988924
5	30	6.109671834156995
5	31	5.683369714851626
5	32	5.844940480675046
5	33	5.475519836618254
5	34	5.880465501415745
5	35	5.935929571164181
5	36	6.2221558272487805
5	37	6.9225918780275855
5	38	7.7416452185366715
5	39	8.583134431121998
5	40	10.395261425571864
5	41	12.158280315247534
5	42	11.47122216277808
5	43	10.238254524593554
5	44	12.090838925772214
5	45	10.991892734617053
5	46	11.571718569340117
5	47	11.839479605014109
5	48	11.911751243876672
5	49	12.725659881004685
5	50	12.284634337588667
5	51	11.534099556800836
5	52	11.59391450330165
5	53	11.397328271568963
5	54	11.173613262296914
5	55	10.006128929330366
5	56	11.005322264416883
6	1	2.007604807343533
6	2	2.6429482035568412
6	3	5.050747107403254
6	4	2.917465169070468
6	5	2.744107052775348
6	6	2.7655338242062086
6	7	2.8513990987160156
6	8	3.133137644915422
6	9	2.91533613180075
6	10	3.646148365424125
6	11	4.85163813546518
6	12	4.99116853492108
6	13	5.955261993816109
6	14	5.411095380770583
6	15	6.546220117391361
6	16	7.367583148401206
6	17	8.556102357133359
6	18	9.300941410227612
6	19	7.669724025503282
6	20	7.323993164220653
6	21	6.888182298073399
6	22	7.739612130534564
6	23	6.835796742173379
6	24	7.236275083376295
6	25	6.762069096641362
6	26	6.274789110456194
6	27	6.1313966346561095
6	28	5.551136547359455
6	29	6.040455184675789
6	30	6.186692036177202
6	31	5.183369714851626
6	32	5.1277687635033296
6	33	5.2419339780323995
6	34	5.074278632728873
6	35	5.368379066113675
6	36	5.83389825149121
6	37	6.2218343022700076
6	38	7.075609865001315
6	39	8.3621748351624
6	40	5.470387688198127
6	41	12.167118699085922
6	42	10.92766155671747
6	43	10.777395938734966
6	44	11.109147006580297
6	45	10.70338263360695
6	46	12.118751397622948
6	47	12.829378594913099
6	48	11.83536235498778
6	49	13.317200285045086
6	50	12.749912115366445
6	51	12.636056627507909
6	52	13.397576119463263
6	53	12.388805544296236
6	54	12.698550130983786
6	55	12.439841050542487
6	56	12.422620244214862
7	1	1.9879527454878598
7	2	2.8815650420448193
7	3	4.701627691595689
7	4	2.9807168872835206
7	5	3.06509072975129
7	6	2.9306970544467603
7	7	2.881682603870658
7	8	2.84447785110099
7	9	3.0315302898763505
7	10	3.526947334496292
7	11	4.658553943025318
7	12	5.529506163787062
7	13	5.66370271546559
7	14	5.647457064619381
7	15	6.824678021171426
7	16	7.368871808195021
7	17	8.957842047855006
7	18	9.421753265897717
7	19	7.9591355375307735
7	20	7.083228559409662
7	21	7.042391920066532
7	22	7.573482405448647
7	23	7.01008797928678
7	24	7.952555152104818
7	25	7.105711708325217
7	26	6.144419694648629
7	27	5.2757265315633255
7	28	5.361810945984885
7	29	5.719471507699847
7	30	6.09176076469954
7	31	4.996084491483931
7	32	4.962820309895083
7	33	4.67535322201865
7	34	5.11830784235087
7	35	4.245312055804398
7	36	6.313064918157874
7	37	6.484291346943547
7	38	6.7626803117367125
7	39	7.832535659904664
7	40	8.74036191500225
7	41	12.157238974000006
7	42	10.590247467370393
7	43	10.810471540109535
7	44	12.147592023762424
7	45	11.234525245290804
7	46	12.221307239547347
7	47	12.37577034749042
7	48	11.768566822341732
7	49	12.861659047931685
7	50	12.192996307806307
7	51	10.797246490050863
7	52	10.883615638363612
7	53	11.360777193780773
7	54	12.093846522736367
7	55	11.591258576315685
7	56	10.912955295761254
8	1	2.225066147549718
8	2	2.8197093719417268
8	3	5.010906042111152
8	4	2.908551938829916
8	5	2.9929257812976857
8	6	2.837913549292125
8	7	2.737352706963435
8	8	2.4836531088329465
8	9	3.2170973001856353
8	10	3.2485968190323717
8	11	4.5657704378706825
8	12	5.343939153477784
8	13	5.869888282475905
8	14	5.472199332660622
8	15	6.6081831758106055
8	16	7.894645004071311
8	17	8.854749264349852
8	18	8.988763575176062
8	19	7.928207702479224
8	20	7.248177013017909
8	21	7.124866146870655
8	22	7.800286529159994
8	23	6.577098288565132
8	24	7.117503605713065
8	25	6.9716910897685125
8	26	6.2681310348548145
8	27	5.244798696511783
8	28	5.289645997531274
8	29	6.193698311823557
8	30	6.2876370533593295
8	31	4.94453809973135
8	32	5.333954330513638
8	33	4.974322294183601
8	34	5.097689285649839
8	35	4.936033705288935
8	36	6.240899969704262
8	37	5.958518151067263
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115	21	3.4179442028353044
115	22	3.7361399083123423
115	23	4.107126107252746
115	24	4.270997305598517
115	25	4.349370683942951
115	26	3.073400221567301
115	27	4.020285523544995
115	28	3.408775436248341
115	29	3.778054391024998
115	30	3.9783587028438667
115	31	3.3659093973913095
115	32	2.842054477789045
115	33	2.6169339780323995
115	34	2.043524664474905
115	35	3.2245298597644663
115	36	5.154334759427716
115	37	5.267469222904932
115	38	5.546840023731473
115	39	6.685587533575095
115	40	8.848363878674316
115	41	8.46275361972084
115	42	8.119129810685724
115	43	6.811126097465127
115	44	-1.685495850562564
115	45	5.8591366018609214
115	46	2.5904775880991373
115	47	-7.107129341594838
115	48	1.546671878797305
115	49	-2.140140984796183
115	50	2.7449517979061255
115	51	3.3984574211586995
115	52	4.003726913114058
115	53	3.3684682427089356
115	54	3.7724588611425176
115	55	2.682896606098044
115	56	6.223215482310099
116	1	1.7433301159855077
116	2	2.344337092445734
116	3	3.741719329625475
116	4	2.5220176382062647
116	5	2.484461991046949
116	6	2.045626416798804
116	7	1.8629731727900918
116	8	2.0096808547919665
116	9	1.6799966256279149
116	10	2.194759476535239
116	11	3.282965295959009
116	12	3.518560510229719
116	13	3.4641354506062356
116	14	4.190030565955773
116	15	-3.2211409937197537
116	16	4.591348580499972
116	17	5.845840011454346
116	18	5.03203708923996
116	19	4.915480198342788
116	20	3.8340240284181846
116	21	5.25392303881414
116	22	5.8472510194234495
116	23	5.112417112543753
116	24	5.900626935228143
116	25	5.169476504048774
116	26	4.480807628974713
116	27	4.427692930952407
116	28	3.9519853127915496
116	29	4.645779258749865
116	30	4.287000678152506
116	31	4.461147492629404
116	32	4.251225553626785
116	33	3.604588299020051
116	34	3.9747415956918353
116	35	4.023471658706271
116	36	4.893311831738121
116	37	5.161649117084824
116	38	6.178233321791442
116	39	7.209397057384621
116	40	8.410974107951215
116	41	8.924834748468637
116	42	6.260223285112534
116	43	2.9240008770065735
116	44	3.3568321917654806
116	45	5.755080164471149
116	46	0.8321001630550455
116	47	-6.232349800148629
116	48	1.1586648241235835
116	49	-1.9937565050783697
116	50	2.09944915240348
116	51	5.283818973186918
116	52	5.098965008352156
116	53	6.040425914666606
116	54	4.696621118638106
116	55	4.707587964122734
116	56	7.849317775079058
117	1	1.2910099797573267
117	2	1.8060229162005186
117	3	3.6689223947595764
117	4	1.8287439047026481
117	5	1.79331682289029
117	6	1.6139533644360995
117	7	1.9440715125091188
117	8	2.2710686793981836
117	9	1.6861694651340855
117	10	2.807786296458609
117	11	1.2776438825916188
117	12	-7.7383573271478845
117	13	-7.963919040666649
117	14	-1.2652695617581493
117	15	-8.305432181459217
117	16	-7.05914098952983
117	17	0.8524385640299101
117	18	-2.3596189345999754
117	19	0.3116062094113268
117	20	0.5387920147953693
117	21	5.294791493475703
117	22	6.17675293513226
117	23	5.293770880104411
117	24	5.78440726728434
117	25	5.509195533422975
117	26	4.353093708157342
117	27	4.217603531207832
117	28	4.72103597264681
117	29	5.318831621457402
117	30	4.702496633878351
117	31	4.171875461978065
117	32	3.869148073848155
117	33	3.766359265388722
117	34	2.396836103993241
117	35	3.568810100596437
117	36	4.744099400916493
117	37	5.145684876982653
117	38	5.755925956955341
117	39	6.487174835162399
117	40	7.864784239922262
117	41	6.553612951959483
117	42	5.543322476257703
117	43	-2.100477624483421
117	44	-0.09272080951165762
117	45	5.724215966940285
117	46	-0.691952625365559
117	47	-3.222345543017937
117	48	0.21826465383835725
117	49	-2.6116790252997433
117	50	0.8152856785848321
117	51	3.8749215700366406
117	52	4.439961176934531
117	53	2.928676233951407
117	54	3.628507027535509
117	55	4.006651395370074
117	56	6.743740933870033
118	1	0.4902436962324188
118	2	1.4554482035568412
118	3	3.3157934036995442
118	4	1.6269559098112083
118	5	1.5091533490716387
118	6	1.601181972354361
118	7	1.3814916913086108
118	8	1.9479524597302387
118	9	1.908391687356307
118	10	2.3799446617204225
118	11	-0.08123223490518683
118	12	-8.604896279893733
118	13	-6.807469487665372
118	14	-7.254413878488673
118	15	-8.684103956682716
118	16	-9.544453888635832
118	17	-1.4566291243481224
118	18	-6.832160441624239
118	19	-1.1092111596819052
118	20	-1.5116549839274924
118	21	4.031700816591918
118	22	4.569473241645671
118	23	3.686491186617822
118	24	4.845071379672586
118	25	3.817624652196919
118	26	5.147474295641377
118	27	4.205470708730186
118	28	4.371738399211303
118	29	4.905038518009125
118	30	4.811692036177202
118	31	3.9981845296664424
118	32	4.36850950424407
118	33	4.450267311365728
118	34	4.82659344754369
118	35	3.9493975846321945
118	36	6.035287140380095
118	37	5.124612080047786
118	38	4.962183939075391
118	39	5.987174835162399
118	40	7.565295095605531
118	41	7.319896476863697
118	42	6.346643038198955
118	43	1.3375811239201525
118	44	1.7086840436173318
118	45	6.076067818792136
118	46	0.5851865828081309
118	47	0.588637854172358
118	48	0.485825317950745
118	49	0.14821880356360495
118	50	3.09944915240348
118	51	6.975176997878275
118	52	7.561927971315118
118	53	6.855240729481423
118	54	8.579337168020825
118	55	7.738452161653598
118	56	10.003638762733381
119	1	1.0854817914705137
119	2	1.741162489271126
119	3	4.122671710577855
119	4	1.9417707246260179
119	5	1.911269751188044
119	6	1.6276369988093862
119	7	1.6936610034779207
119	8	1.9426614544392322
119	9	2.202042481007105
119	10	2.7661880479638086
119	11	1.59866194498899
119	12	-6.393256068253525
119	13	-1.8180514982473817
119	14	-4.217376841451639
119	15	-8.84018861276737
119	16	-9.062972407154351
119	17	-0.04657621429521441
119	18	-6.678721288185088
119	19	-0.4160894665602122
119	20	-0.4269988992714069
119	21	5.052864837755944
119	22	5.990108162280595
119	23	5.297602297728936
119	24	5.52496555956677
119	25	5.333497668069938
119	26	6.295622443789522
119	27	5.655206158465635
119	28	5.265918293391202
119	29	5.762181375151979
119	30	6.152961877447044
119	31	5.302417333899246
119	32	5.143641779376349
119	33	5.505822866921285
119	34	5.122889743839988
119	35	5.510244145478758
119	36	6.614652219745178
119	37	6.870643826079533
119	38	7.245252722144176
119	39	8.733206581194146
119	40	10.610268640579076
119	41	10.256404413371637
119	42	9.246113937669858
119	43	-1.077762791423762
119	44	3.647837482770772
119	45	7.859136601860918
119	46	3.320636318257865
119	47	-1.4563356908011862
119	48	-2.3263439942185684
119	49	-1.5210933657485661
119	50	2.808443861398189
119	51	8.700044722746
119	52	8.940234849621994
119	53	8.892277766518458
119	54	7.677220765904423
119	55	9.682896606098044
119	56	11.683532942627558
120	1	0.8471175192079414
120	2	1.2972561131613602
120	3	3.9488756384767
120	4	1.7138987848896718
120	5	1.557489821136926
120	6	1.6507739372005616
120	7	1.5516109631227977
120	8	1.7941545940679617
120	9	1.7624406515747637
120	10	2.6938178569495506
120	11	-0.2673590396760588
120	12	-6.247708583722989
120	13	-6.6988693621160955
120	14	-4.560126370641843
120	15	-0.44336321594197514
120	16	-7.9650439702428635
120	17	0.4909540520486111
120	18	-5.149800115196118
120	19	-0.3421050705419191
120	20	-0.2844107905816031
120	21	3.8634647839491087
120	22	4.70977455991309
120	23	3.572555216749649
120	24	3.8340858178395685
120	25	4.553029548618767
120	26	4.357769336444893
120	27	3.978854261774753
120	28	4.220451519110867
120	29	4.34571648411082
120	30	4.537680736742171
120	31	3.864160675303605
120	32	3.5514975770626513
120	33	4.232753187071943
120	34	4.192569593180853
120	35	3.7698621169611357
120	36	4.7537428842595695
120	37	4.629320177976222
120	38	5.5011042152838066
120	39	7.190564665670877
120	40	7.285006332265926
120	41	-5.299689210518601
120	42	-0.12353900825427644
120	43	-7.031219880474072
120	44	-14.246432089464903
120	45	-2.436800982212258
120	46	-5.272314986557845
120	47	-5.91638411695131
120	48	-3.0675331252382065
120	49	-14.294028528514236
120	50	-1.813294099322821
120	51	3.5426597348525384
120	52	3.4925619951694813
120	53	3.6192080866691185
120	54	3.6185713174244647
120	55	4.212086813254352
120	56	7.476645667943675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1119.0
5	48.0
6	193.0
7	249.0
8	387.0
9	399.0
10	286.0
11	253.0
12	200.0
13	141.0
14	154.0
15	186.0
16	231.0
17	291.0
18	394.0
19	470.0
20	631.0
21	822.0
22	1075.0
23	1364.0
24	1658.0
25	1829.0
26	2047.0
27	2205.0
28	2294.0
29	2421.0
30	2609.0
31	3043.0
32	3429.0
33	3839.0
34	4230.0
35	4550.0
36	4915.0
37	5686.0
38	6180.0
39	6815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.68788920066623	0.2280809687439041	0.060021307564185285	0.024008523025674116
8	99.65187641612772	0.23858469756763653	0.07202556907702234	0.037513317227615806
9	97.46852538227218	0.7487882834891433	1.2274725769421226	0.5552137572965592
10	42.72166619149798	27.893402157766005	20.954939003346187	8.429992647389824
11	28.9917920861906	25.54806956469547	19.457407379619767	26.00273096949417
12	28.475608841138605	24.520204672658792	18.632114400612217	28.372072085590382
13	21.41560253890131	14.390108488513423	21.56415527512267	42.6301336974626
14	21.448614258061614	31.880317512717017	25.03038578695437	21.640682442267003
15	37.31055179382003	18.595708102728054	20.969371597472517	23.1243685059794
16	29.59176013092503	29.321502034442894	22.228728435656052	18.858009398976023
17	26.17564504750612	18.16189604190745	35.941041382105276	19.721417528481155
18	22.625031886319643	18.022898128835738	25.581081283855767	33.77098870098885
19	25.299866392445917	20.715175716451746	21.60088871541591	32.38406917568643
20	21.732401772969727	24.699872286079184	32.41379310344827	21.15393283750282
21	21.070480020407246	19.329862101045872	27.468751406749398	32.13090647179749
22	19.382380745164536	18.719145296580287	35.23400807286587	26.664465885389316
23	19.386882343231846	21.433608931170568	31.59371576909803	27.585792956499557
24	19.4768750562742	21.574777154176296	27.014616285002553	31.93373150454695
25	25.981451467675132	21.348820457410408	31.071793024791404	21.597935050123056
26	35.289877922428936	21.754733696713068	19.762151448263435	23.19323693259456
27	22.097580754155967	30.448559114595064	19.28939346157889	28.164466669670073
28	27.048725220966702	30.594696799171654	20.43848197001756	21.918096009844085
29	34.513753194047794	25.967232827295955	19.35818427776943	20.16082970088682
30	29.30817043509594	21.256343272377865	29.794613097919104	19.640873194607092
31	30.01725819764388	29.870188339461244	21.281608764162975	18.830944698731898
32	34.170831644828674	26.12604499677308	20.662794362645776	19.04032899575247
33	30.550087899869276	25.815515453848814	21.037368713656786	22.597027932625128
34	22.93480058329199	25.52052796945234	33.34084998271171	18.203821464543964
35	21.463678585922352	23.46575280696217	36.83846627887751	18.232102328237964
36	27.840747023764845	25.998708921949827	27.222230562519705	18.938313491765623
37	27.617043661230934	21.946344029458178	30.538814157961973	19.897798151348915
38	31.98917048958412	23.179664585996843	25.04324283673009	19.78792208768895
39	27.982630123812957	21.85659334054574	20.839343671114317	29.321432864526987
40	23.030495552731896	16.99794276033158	28.725721546560173	31.24584014037635
41	20.017596553502624	15.71099177816207	25.35420648645369	38.91720518188162
42	23.383855867788498	28.00012041120426	22.167702704736676	26.448321016270565
43	17.274902556848108	24.031964363647308	31.394001414618728	27.299131664885852
44	20.872645064054257	25.20422004521477	25.642803315749813	28.28033157498116
45	25.576463155994766	21.62357294345923	30.720635350390324	22.07932855015568
46	19.194412667830694	28.772051297489316	22.37190679751942	29.661629237160575
47	17.624786930746836	26.992291795513857	23.153274101338	32.22964717240131
48	25.22821452165035	25.59305747402558	23.16977959281725	26.008948411506815
49	19.543429844097997	28.242942274122672	21.602058628784686	30.61156925299464
50	23.290183873829175	27.611143686196684	23.48713785275059	25.61153458722355
51	19.73929797097959	30.435306159311214	25.45758323800349	24.3678126317057
52	19.96692723992784	32.79314491882141	26.57696933253157	20.662958508719182
53	20.30845621426422	29.331928979837496	28.23803791754439	22.121576888353896
54	20.467466835123183	26.225069939536144	28.18939325572301	25.11806996961766
55	19.296397724878872	25.783201420445995	29.552499322880614	25.36790153179452
56	22.63081111161245	21.84120681616508	30.44112559972326	25.086856472499207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	2.5
7	5.0
8	24.0
9	43.0
10	75.0
11	107.0
12	117.0
13	130.5
14	134.0
15	138.5
16	143.0
17	132.0
18	121.0
19	130.5
20	140.0
21	160.5
22	198.5
23	216.0
24	259.5
25	303.0
26	336.5
27	370.0
28	383.0
29	447.0
30	498.0
31	559.0
32	620.0
33	755.0
34	890.0
35	958.5
36	1027.0
37	1063.5
38	1239.5
39	1379.0
40	1667.5
41	1956.0
42	2565.5
43	3175.0
44	4702.5
45	6230.0
46	6353.0
47	5438.5
48	4401.0
49	4184.5
50	3968.0
51	4025.5
52	4083.0
53	4157.5
54	4012.0
55	3792.0
56	3901.5
57	4011.0
58	4019.0
59	4027.0
60	3676.5
61	3326.0
62	2907.5
63	2289.5
64	2090.0
65	1727.0
66	1364.0
67	1174.5
68	985.0
69	860.0
70	735.0
71	669.0
72	438.5
73	274.0
74	215.0
75	156.0
76	149.5
77	143.0
78	196.0
79	156.0
80	63.0
81	36.5
82	10.0
83	6.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0030010653782092645
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4981768527827378
16	0.060021307564185285
17	0.030010653782092642
18	0.0
19	0.045015980673138965
20	0.13204687664120765
21	0.0
22	0.0
23	0.0
24	0.007502663445523161
25	0.010503728823732425
26	0.06902450369881308
27	0.07802769983344088
28	0.006002130756418529
29	0.16956019386882343
30	0.05551970949687139
31	0.012004261512837058
32	0.024008523025674116
33	0.1350479420194169
34	0.18606605344897437
35	0.1680596611797188
36	0.04801704605134823
37	0.16205753042330026
38	0.23708416487853187
39	0.13654847470852152
40	0.8027849886709783
41	1.0818840688444398
42	0.306108668577345
43	0.29110334168629864
44	0.4396560779076572
45	0.24008523025674114
46	0.31061026664465885
47	0.5251864411866213
48	0.05852077487508065
49	0.28660174361898477
50	0.19506924958360217
51	0.31061026664465885
52	0.18456552075986973
53	0.2745974821061477
54	0.23558363218942727
55	0.2775985474843569
56	0.23108203412211337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	66643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.42279009048212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.74317419241649	60.72055579730804
2	6.594442860589048	9.024203592275258
3	1.8246014166977347	3.7453295920051617
4	0.8355446391368232	2.2868118181954595
5	0.425447926489616	1.4555167084314933
6	0.30483124629926095	1.2514442627132634
7	0.21491699379372356	1.0293654247257775
8	0.1600912300708349	0.8763110904371051
9	0.14035395513059498	0.864306828924268
>10	0.6688743174192416	8.134387707636211
>50	0.04824667207614202	2.381345377609051
>100	0.032895458233733195	3.8233572918386027
>500	0.004386061097831093	2.676950317362664
>1k	0.0021930305489155466	1.730114190537641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1153	1.730114190537641	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	945	1.4180033912038774	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	839	1.2589469261587862	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	309	0.4636646009333313	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	276	0.41414702219287847	No Hit
TCACTTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	239	0.3586273126960071	No Hit
TCACTTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	209	0.3136113320228681	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	187	0.2805996128625662	No Hit
TCACTTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	151	0.22658043605479944	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	150	0.22507990336569483	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	146	0.21907777260927627	No Hit
TCACTTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	142	0.2130756418528578	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	131	0.1965697822727068	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	131	0.1965697822727068	No Hit
TCACTTGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	128	0.19206818420539293	No Hit
TCACTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	124	0.18606605344897437	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	115	0.1725612592470327	No Hit
TCACTTGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	110	0.16505859580150953	No Hit
TCACTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG	98	0.14705220353225396	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	95	0.14255060546494006	No Hit
TCACTTGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	95	0.14255060546494006	No Hit
TCACTTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	94	0.14105007277583542	No Hit
TCACTTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	90	0.1350479420194169	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	87	0.130546343952103	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	86	0.12904581126299836	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	81	0.1215431478174752	No Hit
TCACTTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	77	0.11554101706105668	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	74	0.11103941899374278	No Hit
TCACTTGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	72	0.1080383536155335	No Hit
TCACTTGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG	70	0.10503728823732425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0015005326891046322	0.0	0.0	0.0	0.0
10	0.009003196134627793	0.0	0.0	0.0	0.0
11	0.015005326891046321	0.0	0.0	0.0	0.0
12	0.016505859580150953	0.0	0.0	0.0	0.0
13	0.01950692495836022	0.0	0.0	0.0	0.0
14	0.07202556907702234	0.0	0.0	0.0	0.0
15	0.07652716714433624	0.0	0.0	0.0	0.0
16	0.0795282325225455	0.0	0.0	0.0	0.0
17	0.08252929790075476	0.0	0.0	0.0	0.0
18	0.0885314286571733	0.0	0.0	0.0	0.0
19	0.09153249403538256	0.0	0.0	0.0	0.0
20	0.10053569017001035	0.0	0.0	0.0	0.0
21	0.10203622285911498	0.0	0.0	0.0	0.0
22	0.10503728823732425	0.0	0.0	0.0	0.0
23	0.11404048437195204	0.0	0.0	0.0	0.0
24	0.12304368050657984	0.0	0.0	0.0	0.0
25	0.12304368050657984	0.0	0.0	0.0	0.0
26	0.12904581126299836	0.0	0.0	0.0	0.0
27	0.14105007277583542	0.0	0.0	0.0	0.0
28	0.15455486697777712	0.0	0.0	0.0	0.0
29	0.1575559323559864	0.0	0.0	0.0	0.0
30	0.16055699773419563	0.0	0.0	0.0	0.0
31	0.18756658613807903	0.0	0.0	0.0	0.0
32	0.19356871689449753	0.0	0.0	0.0	0.0
33	0.2010713803400207	0.0	0.0	0.0	0.0
34	0.2055729784073346	0.0	0.0	0.0	0.0
35	0.2055729784073346	0.0	0.0	0.0	0.0
36	0.20707351109643923	0.0	0.0	0.0	0.0
37	0.2100745764746485	0.0	0.0	0.0	0.0
38	0.21307564185285777	0.0	0.0	0.0	0.0
39	0.2145761745419624	0.0	0.0	0.0	0.0
40	0.21757723992017167	0.0	0.0	0.0	0.0
41	0.22507990336569483	0.0	0.0	0.0	0.0
42	0.22808096874390407	0.0	0.0	0.0	0.0
43	0.24008523025674114	0.0	0.0	0.0	0.0
44	0.2655942859715199	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCA	35	4.894173E-8	50.3713	41
CCCCGAC	40	2.4501787E-9	50.3713	41
TCCTAGA	15	0.008178537	50.333084	40
TCTAATC	15	0.008178537	50.333084	40
GTCATGG	15	0.008203118	50.29492	39
CCGATTG	35	4.95329E-8	50.29492	39
AGCCCTG	15	0.008203118	50.29492	38
TTGCTCA	20	4.036212E-4	50.29492	37
GTCTAAT	20	4.036212E-4	50.29492	39
GCCGATT	35	4.95329E-8	50.29492	38
AGCGCTT	15	0.008203118	50.29492	37
TGCTCAA	20	4.036212E-4	50.29492	38
TTGCATT	15	0.008203118	50.29492	39
GGTAATC	20	4.0665444E-4	50.218777	35
GTAATCC	15	0.008252447	50.218777	36
GAGCGCT	15	0.008252447	50.218777	36
CACTGTT	15	0.008326852	50.104984	43
TGCCTAG	15	0.008326852	50.104984	43
TCTCCAC	20	4.1123823E-4	50.104984	43
TAATCCG	15	0.008326852	50.104984	42
>>END_MODULE