FastQCFastQC Report
Fri 17 Jun 2016
SRR1525985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23031
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2551.1072033346359256No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT1110.48195909860622643No Hit
TCACTTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC770.3343319873214363No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT760.32999001346011897No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC750.3256480395988016No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT750.3256480395988016No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG540.23446658851113714No Hit
TCACTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT460.1997307976205983No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT430.18670487603664626No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG410.17802092831401156No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT410.17802092831401156No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT410.17802092831401156No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC330.1432851374234727No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC320.13894316356215536No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG320.13894316356215536No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC290.12591724197820328No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT260.11289132039425122No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT260.11289132039425122No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC250.10854934653293387No Hit
TCACTTGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT240.10420737267161652No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTCGCCTCTGCT240.10420737267161652No Hit
TCACTTGGGAGTGCAGTGCTTCACTTGGGTCTCATAGCCT240.10420737267161652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAGT200.002685510634.1203547
ACTTGGT251.9263847E-434.1203543
CCTGCCA850.034.12035422
CTCCACC200.002685510634.12035422
TTGGGTG850.034.1203545
CTTGGAA200.002685510634.1203544
TGGGGTA200.002685510634.1203546
CCCGTAG407.2635885E-834.12035426
TTGGGAT1000.034.1203545
TTGGGAC800.034.1203545
GGGGGAA251.9263847E-434.1203547
GGGATGC251.9263847E-434.1203547
GGGACAG200.002685510634.1203547
TGGGTGT407.2635885E-834.1203546
TGGGTGG251.9263847E-434.1203546
TGGGCAA200.002685510634.1203546
GCCCGTA407.2635885E-834.12035425
GGGAAGT455.2732503E-934.1203547
CTTGAGA200.002685510634.1203544
TGGGATT251.9263847E-434.1203546
Produced by FastQC (version 0.11.5)