##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23031
Sequences flagged as poor quality	0
Sequence length	40
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.015414007207678	32.0	30.0	33.0	26.0	35.0
2	16.299986974078415	13.0	11.0	20.0	4.0	31.0
3	19.22899570144588	20.0	13.0	26.0	4.0	30.0
4	22.341669923147062	25.0	14.0	31.0	4.0	33.0
5	22.316964091876166	24.0	17.0	30.0	4.0	33.0
6	24.756111328209805	27.0	21.0	32.0	4.0	35.0
7	24.580174547349223	28.0	21.0	32.0	4.0	35.0
8	22.907125179106423	26.0	17.0	30.0	4.0	34.0
9	24.138812904346317	27.0	20.0	31.0	4.0	34.0
10	19.450740306543356	20.0	10.0	28.0	4.0	33.0
11	25.184143111458468	30.0	17.0	35.0	4.0	38.0
12	25.25105292866137	30.0	17.0	35.0	4.0	38.0
13	25.801094177413052	30.0	19.0	35.0	4.0	38.0
14	26.068255829099908	31.0	19.0	36.0	4.0	38.0
15	24.132343363292954	30.0	12.0	35.0	4.0	38.0
16	21.909209326559854	26.0	4.0	33.0	4.0	36.0
17	22.26542486214233	27.0	4.0	33.0	4.0	36.0
18	21.570144587729583	26.0	4.0	33.0	4.0	36.0
19	18.418609699969608	19.0	4.0	30.0	4.0	35.0
20	20.250705570752466	24.0	4.0	33.0	4.0	36.0
21	16.68859363466632	15.0	4.0	29.0	4.0	34.0
22	16.512005557726543	14.0	4.0	30.0	4.0	34.0
23	16.530545786114367	14.0	4.0	30.0	4.0	34.0
24	16.014719291389866	10.0	4.0	30.0	4.0	34.0
25	15.369067778211976	4.0	4.0	30.0	4.0	34.0
26	12.36133906473883	4.0	4.0	23.0	4.0	32.0
27	11.606269810255743	4.0	4.0	20.0	4.0	31.0
28	11.288220224914246	4.0	4.0	20.0	4.0	31.0
29	10.440623507446485	4.0	4.0	16.0	4.0	30.0
30	9.855976727020103	4.0	4.0	13.0	4.0	30.0
31	9.507142547001868	4.0	4.0	8.0	4.0	30.0
32	9.113759715166514	4.0	4.0	4.0	4.0	29.0
33	9.097477313186575	4.0	4.0	4.0	4.0	29.0
34	8.856541183622074	4.0	4.0	4.0	4.0	29.0
35	8.504233424514785	4.0	4.0	4.0	4.0	28.0
36	8.409274456167774	4.0	4.0	4.0	4.0	30.0
37	8.38061742868308	4.0	4.0	4.0	4.0	30.0
38	8.095783943380662	4.0	4.0	4.0	4.0	29.0
39	7.954018496808649	4.0	4.0	4.0	4.0	29.0
40	7.857409578394338	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-11.035196343738566
1	2	-10.927713334192738
1	3	-14.353219420457485
1	4	-16.786568316846147
1	5	-17.034281614388043
1	6	-19.055585074229008
1	7	-18.813893892207453
1	8	-17.595377123608554
1	9	-18.157352273001056
1	10	-14.487856237821187
1	11	-19.847569513411074
1	12	-19.270404276029534
1	13	-20.500739701294073
1	14	-19.94062249702794
1	15	-18.308103568467423
1	16	-16.361055292620556
1	17	-16.743495205613677
1	18	-15.729159465036595
1	19	-13.044991443788884
1	20	-14.141234274694972
1	21	-9.490865490991258
1	22	-9.630937724134295
1	23	-10.737264134054312
1	24	-9.001086613905006
1	25	-8.432814481155809
1	26	-7.09701251983892
1	27	-5.701049054790002
1	28	-5.146858675659886
1	29	-5.465596264418234
1	30	-5.285303723339856
1	31	-3.6879148881284785
1	32	-3.2261955392404804
1	33	-3.866019922600862
1	34	-3.1491106366445187
1	35	-2.9003522959156394
1	36	-2.9611950673826604
1	37	-2.5733072516497835
1	38	-2.5128688357327356
1	39	-2.2505649164044197
1	40	-1.9545800728205354
5	1	8.118649810107588
5	2	21.79023538375598
5	3	-0.6609117281497916
5	4	16.29035476007693
5	5	15.811872231765804
5	6	13.329030310386376
5	7	13.698926620613062
5	8	15.173853645622216
5	9	13.996493880845097
5	10	13.563425813460864
5	11	-4.078338744180304
5	12	4.780877775252517
5	13	4.268491067936697
5	14	-7.017545573951018
5	15	7.897024636660781
5	16	7.228688297123032
5	17	-3.3075977697162386
5	18	-5.062492798369927
5	19	5.647316248518809
5	20	0.42286828940759236
5	21	3.5091345090087422
5	22	16.44598535278878
5	23	8.288376891586713
5	24	15.998913386094994
5	25	-10.48409653243786
5	26	-8.020089442915843
5	27	-6.931818285559233
5	28	-6.89044841924963
5	29	-6.234827033649003
5	30	-5.798124236160369
5	31	-5.277658477872068
5	32	-4.81593912898407
5	33	-5.045507102088042
5	34	-4.533726021259904
5	35	-4.13112152668487
5	36	-4.243246349433942
5	37	-4.163050841393373
5	38	-3.7949201177840175
5	39	-3.7633854292249325
5	40	-3.5699646882051503
6	1	-4.734291366362999
6	2	-2.8862352044793127
6	3	1.044970624791386
6	4	-9.297880534040717
6	5	-2.541068944704783
6	6	-0.8768520425548019
6	7	-1.1834263205634095
6	8	-3.5614404720248416
6	9	-1.8270355309196091
6	10	0.38695522522557013
6	11	-2.1959858030038326
6	12	-2.3661810482768963
6	13	-0.5550383438280093
6	14	-0.1940161621863119
6	15	-2.073563598633335
6	16	-4.241899938171084
6	17	0.2806375244014099
6	18	0.8786836722183082
6	19	2.382610366165867
6	20	1.4228682894075924
6	21	5.626781567832271
6	22	5.710691235141724
6	23	7.847200420998478
6	24	5.793031033153817
6	25	5.751197585209198
6	26	6.244616439437099
6	27	4.597593479146649
6	28	5.344845698397428
6	29	2.147525907527468
6	30	2.613640469721984
6	31	0.5458709338926386
6	32	1.0075902827806367
6	33	0.7780223096766647
6	34	0.936862214034214
6	35	-1.601709761978988
6	36	-0.007952231786884312
6	37	0.21930209978309811
6	38	0.9991975292748068
6	39	0.2660263354809498
6	40	0.7535647235595562
7	1	1.7686498101075863
7	2	0.31523538375597937
7	3	7.139088271850209
7	4	2.6153547600769294
7	5	6.511872231765803
7	6	3.9790303103863742
7	7	2.84892662061306
7	8	2.523853645622218
7	9	3.6964938808450967
7	10	9.013425813460863
7	11	5.371661255819696
7	12	6.455877775252517
7	13	8.043491067936696
7	14	6.88245442604898
7	15	10.147024636660781
7	16	10.053688297123031
7	17	9.892402230283764
7	18	7.587507201630071
7	19	9.82231624851881
7	20	10.672868289407592
7	21	14.03413450900874
7	22	14.870985352788782
7	23	15.363376891586713
7	24	15.998913386094994
7	25	14.06590346756214
7	26	8.029910557084158
7	27	7.0431817144407685
7	28	12.30955158075037
7	29	10.790172966350996
7	30	11.176875763839632
7	31	4.572341522127932
7	32	3.8840608710159295
7	33	2.8544928979119586
7	34	3.2162739787400962
7	35	-0.7561215266848702
7	36	0.456753650566057
7	37	-0.11305084139337218
7	38	0.9050798822159818
7	39	-0.21338542922493264
7	40	0.10503531179484948
8	1	-2.2659655745077956
8	2	-0.44053384701324916
8	3	0.05703698979892735
8	4	0.49548296520513446
8	5	-5.034281614388043
8	6	2.149543130899197
8	7	1.3655932872797294
8	8	2.6097510815196507
8	9	0.04777593212714848
8	10	-4.077599827564777
8	11	1.998584332742773
8	12	2.806518800893542
8	13	2.9095167089623395
8	14	0.7260441696387261
8	15	-2.436308696672551
8	16	0.02356009199482756
8	17	-8.281956744075213
8	18	-0.908646644523774
8	19	-0.37832477712221646
8	20	0.16645803299733686
8	21	1.8937498936241255
8	22	1.5485494553528838
8	23	3.13453073774056
8	24	3.8707082578898664
8	25	-3.817429865771194
8	26	-0.7893202121466132
8	27	-1.2138695676105158
8	28	-0.5827561115573214
8	29	-2.8245706233925922
8	30	-2.4904319284680616
8	31	-2.816120016333606
8	32	-1.9954263084712496
8	33	-1.814737871318811
8	34	-1.3798798674137496
8	35	-0.977275372838716
8	36	-0.1919642981518912
8	37	-1.2399739183164495
8	38	0.025592702728802763
8	39	0.28789662205711863
8	40	-0.3904775087179706
9	1	4.777740719198494
9	2	9.403871747392344
9	3	9.498179180941118
9	4	10.29035476007693
9	5	9.19823586812944
9	6	9.488121219477282
9	7	11.767108438794882
9	8	12.832944554713123
9	9	11.45103933539055
9	10	12.85888035891541
9	11	4.44438852854697
9	12	4.939968684343427
9	13	6.154854704300334
9	14	2.164272607867165
9	15	4.397024636660781
9	16	7.660506478941215
9	17	9.124220412101945
9	18	8.392052656175526
9	19	7.238225339427899
9	20	8.786504653043956
9	21	13.418225418099652
9	22	8.741439898243328
9	23	13.08383143704126
9	24	14.658004295185904
9	25	13.834085285743956
9	26	3.5026378298114302
9	27	4.522727168986222
9	28	3.6322788534776436
9	29	2.356082057260089
9	30	3.5200575820214492
9	31	1.1087051584915688
9	32	4.18406087101593
9	33	2.4090383524574133
9	34	1.7617285241946412
9	35	1.8916057460424032
9	36	2.7340263778387843
9	37	3.6551309767884455
9	38	1.9550798822159825
9	39	3.963887298047794
9	40	3.339126220885758
10	1	-0.3813501898924123
10	2	-2.209764616244019
10	3	-6.160911728149792
10	4	6.29035476007693
10	5	-1.188127768234196
10	6	4.329030310386376
10	7	4.6989266206130615
10	8	7.173853645622216
10	9	4.996493880845097
10	10	5.063425813460864
10	11	10.921661255819696
10	12	9.280877775252517
10	13	7.768491067936697
10	14	8.482454426048982
10	15	0.897024636660781
10	16	14.728688297123032
10	17	14.692402230283761
10	18	13.437507201630073
10	19	18.14731624851881
10	20	11.922868289407592
10	21	16.009134509008742
10	22	15.945985352788782
10	23	17.288376891586715
10	24	11.498913386094994
10	25	18.51590346756214
10	26	6.979910557084157
10	27	-6.931818285559233
10	28	-6.89044841924963
10	29	-6.234827033649003
10	30	-5.798124236160369
10	31	-5.277658477872068
10	32	-4.81593912898407
10	33	-5.045507102088042
10	34	-4.533726021259904
10	35	-4.13112152668487
10	36	-4.243246349433942
10	37	-4.163050841393373
10	38	-3.7949201177840175
10	39	-3.7633854292249325
10	40	-3.5699646882051503
11	1	3.6186498101075877
11	2	3.974445910071772
11	3	7.181193535008102
11	4	0.7903547600769301
11	5	8.443451179134225
11	6	8.592188205123218
11	7	8.804189778507801
11	8	11.226485224569586
11	9	8.285967565055625
11	10	9.379215287145072
11	11	7.895345466346011
11	12	6.5966672489367255
11	13	6.400070015305115
11	14	6.956138636575297
11	15	4.31807726823973
11	16	9.412898823438823
11	17	10.902928546073234
11	18	9.832244043735336
11	19	8.27889519588723
11	20	11.738657763091801
11	21	12.667029245850848
11	22	7.182827458051939
11	23	13.841008470534083
11	24	11.209439701884467
11	25	10.726429783351612
11	26	6.216752662347314
11	27	2.515550135493399
11	28	3.899025264960896
11	29	1.212541387403629
11	30	1.3334547112080521
11	31	0.43286783791740646
11	32	0.6840608710159302
11	33	0.6913350031751158
11	34	0.5715371366348325
11	35	1.0794047891046041
11	36	0.2567536505660577
11	37	-1.0841034729723207
11	38	0.02086935590019312
11	39	0.026088254985594084
11	40	0.19319320653169214
12	1	-1.7342913663629993
12	2	-2.8568234397734305
12	3	1.2214412130266794
12	4	-5.356704063452483
12	5	2.7530487023540395
12	6	3.740795016268727
12	7	3.0812795617895326
12	8	7.115030116210452
12	9	2.2611997631980394
12	10	2.9457787546373346
12	11	9.186367138172638
12	12	10.016171892899575
12	13	8.53319695028964
12	14	8.511866190754866
12	15	8.073495224896078
12	16	13.169864767711264
12	17	11.457108112636703
12	18	12.79044837810066
12	19	12.853198601459987
12	20	15.187574171760534
12	21	17.009134509008742
12	22	17.387161823377014
12	23	15.11190630335142
12	24	18.057736915506762
12	25	17.662962291091553
12	26	5.244616439437099
12	27	3.7152405379701783
12	28	8.10955158075037
12	29	3.529878848703939
12	30	4.201875763839631
12	31	1.6046944633044031
12	32	1.2428844004276947
12	33	0.33684583908842924
12	34	2.1133328022695075
12	35	1.1041725909621878
12	36	0.8155771799778222
12	37	3.2781256291948626
12	38	2.4991975292748068
12	39	4.060143982539774
12	40	3.606505900030143
13	1	-5.828718610945042
13	2	0.8428669627033507
13	3	-10.292490675518213
13	4	-15.604382082028334
13	5	-12.661811978760511
13	6	-11.51307495277152
13	7	-11.616862853071149
13	8	-9.61562003858831
13	9	-11.161400855997007
13	10	-9.752363660223347
13	11	-5.920444007338197
13	12	-5.113859066852747
13	13	-4.889403668905409
13	14	-5.754387679214176
13	15	-4.471396415970798
13	16	-2.771311702876968
13	17	-2.2549661907688687
13	18	-2.5361770088962423
13	19	-1.1947890146390847
13	20	-4.629763289539776
13	21	-1.122444438359679
13	22	-0.027698857737533444
13	23	-2.395833634729076
13	24	-0.6852971402207952
13	25	-1.6946228482273344
13	26	-1.6516683902842644
13	27	-3.405502496085549
13	28	-3.1536063139864723
13	29	-1.7085112441753179
13	30	-4.061282130897212
13	31	-3.5408163726089104
13	32	-3.1843601816156486
13	33	-2.4665597336669896
13	34	-2.4810944423125356
13	35	-2.3942794214217127
13	36	0.38833259793447894
13	37	-1.215682420340741
13	38	0.6261325137949294
13	39	0.5524040444592782
13	40	0.7458247854790603
15	1	4.550467991925768
15	2	5.653871747392344
15	3	9.498179180941118
15	4	0.7903547600769301
15	5	10.766417686311257
15	6	9.579030310386376
15	7	10.471653893340335
15	8	11.992035463804037
15	9	10.610130244481464
15	10	11.9497894498245
15	11	6.19438852854697
15	12	5.758150502525243
15	13	7.586672886118517
15	14	7.959727153321708
15	15	6.5788428184789645
15	16	8.319597388032122
15	17	8.669674957556488
15	18	6.846598110720983
15	19	7.874588975791536
15	20	11.127413743953046
15	21	10.986407236281469
15	22	7.991439898243328
15	23	11.788376891586713
15	24	14.06709520427681
15	25	8.197721649380323
15	26	10.025365102538704
15	27	6.227272623531677
15	28	3.7231879443867335
15	29	5.742445693623724
15	30	3.0882394002032676
15	31	3.904159703946114
15	32	6.138606325561385
15	33	4.79540198882105
15	34	5.23900125146737
15	35	4.346151200587856
15	36	3.370390014202421
15	37	3.4051309767884455
15	38	3.364170791306891
15	39	1.7138872980477942
15	40	4.884580766340305
16	1	3.6767893449913096
16	2	2.6274446860815637
16	3	8.222809202082768
16	4	10.29035476007693
16	5	9.021174557347198
16	6	9.119727984804978
16	7	8.489624295031664
16	8	10.29013271538966
16	9	8.368586904100912
16	10	10.284356046019003
16	11	6.805382186052256
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115	6	9.329030310386376
115	7	9.698926620613062
115	8	10.173853645622216
115	9	9.996493880845097
115	10	14.563425813460864
115	11	3.9216612558196964
115	12	12.780877775252517
115	13	12.268491067936697
115	14	11.982454426048982
115	15	13.897024636660781
115	16	9.228688297123032
115	17	-0.30759776971623864
115	18	9.937507201630073
115	19	12.647316248518809
115	20	11.422868289407592
115	21	-11.490865490991258
115	22	-11.554014647211218
115	23	-11.711623108413287
115	24	-11.001086613905006
115	25	-10.48409653243786
115	26	-8.020089442915843
115	27	-6.931818285559233
115	28	-6.89044841924963
115	29	-6.234827033649003
115	30	-5.798124236160369
115	31	-5.277658477872068
115	32	-4.81593912898407
115	33	-5.045507102088042
115	34	-4.533726021259904
115	35	-4.13112152668487
115	36	-4.243246349433942
115	37	-4.163050841393373
115	38	-3.7949201177840175
115	39	-3.7633854292249325
115	40	-3.5699646882051503
116	1	-0.3575406660828868
116	2	-4.019288425767829
116	3	-1.3275783948164595
116	4	-9.042978573256404
116	5	0.43091985081342443
116	6	-2.861445880089814
116	7	1.222736144422587
116	8	-1.873765401996831
116	9	-1.1939823096310924
116	10	-0.5318122817772313
116	11	-0.12595779179935107
116	12	1.0665920609668014
116	13	1.1256339250795548
116	14	0.22054966414421884
116	15	2.325596065232208
116	16	1.133450201884937
116	17	3.216211754093287
116	18	2.556554820677693
116	19	-5.019350418147857
116	20	-0.10094123440193314
116	21	2.2234202232944575
116	22	0.017413924217352772
116	23	-0.3306707274609053
116	24	4.665580052761662
116	25	1.6111415628002348
116	26	-1.210565633392033
116	27	0.9253245715836247
116	28	-0.8428293716305824
116	29	-0.044350843172813015
116	30	-0.17907661711275047
116	31	1.3413891411755507
116	32	1.7078703948254539
116	33	1.7640167074357684
116	34	2.1805596930258098
116	35	2.9641165685532247
116	36	2.8519917458041526
116	37	2.027425349082817
116	38	-0.842539165403065
116	39	0.9032812374417336
116	40	0.7633686451281836
117	1	-1.3813501898924123
117	2	-4.9097646162440185
117	3	2.68908827185021
117	4	-12.00964523992307
117	5	1.2118722317658026
117	6	0.5790303103863756
117	7	-0.9010733793869399
117	8	-7.976146354377784
117	9	-4.203506119154902
117	10	2.663425813460865
117	11	-1.3783387441803043
117	12	-2.019122224747484
117	13	-4.881508932063301
117	14	-5.317545573951019
117	15	-2.1529753633392197
117	16	-2.271311702876968
117	17	-2.2075977697162372
117	18	0.6875072016300727
117	19	0.8973162485188091
117	20	0.32286828940759094
117	21	2.7591345090087422
117	22	5.145985352788781
117	23	3.2383768915867126
117	24	-0.45108661390500515
117	25	1.26590346756214
117	26	-3.1700894429158435
117	27	-3.5818182855592333
117	28	-5.04044841924963
117	29	-3.8348270336490025
117	30	-4.548124236160369
117	31	-3.8776584778720675
117	32	-2.6659391289840695
117	33	-3.0455071020880418
117	34	-3.2837260212599038
117	35	-3.5811215266848704
117	36	-2.6932463494339425
117	37	-2.913050841393373
117	38	-2.944920117784018
117	39	-2.2133854292249326
117	40	-2.11996468820515
118	1	-3.9480168565590787
118	2	-6.143097949577353
118	3	-4.194245061483125
118	4	-11.642978573256404
118	5	-1.5214611015675281
118	6	-6.737636356280291
118	7	-14.034406712720271
118	8	-2.759479687711117
118	9	-9.936839452488236
118	10	-1.5032408532058028
118	11	-5.345005410846969
118	12	-6.485788891414149
118	13	-11.99817559872997
118	14	-11.950878907284352
118	15	-11.369642030005886
118	16	-9.771311702876968
118	17	-8.374264436382905
118	18	-8.662492798369927
118	19	-5.9526837514811906
118	20	-5.910465043925742
118	21	-3.0908654909912574
118	22	-0.487347980544552
118	23	-1.911623108413286
118	24	-2.2677532805716734
118	25	-1.8840965324378605
118	26	-1.48675610958251
118	27	-0.9984849522258994
118	28	-0.3571150859162966
118	29	0.2985062996843304
118	30	1.9352090971729634
118	31	-1.7443251445387347
118	32	-1.2826057956507366
118	33	-0.31217376875470926
118	34	0.3996073120734298
118	35	-2.4644548600182032
118	36	-0.9765796827672757
118	37	-1.2297175080600393
118	38	-1.1282534511173505
118	39	-0.9633854292249326
118	40	-2.703298021538484
119	1	-25.881350189892412
119	2	-12.20976461624402
119	3	-14.660911728149792
119	4	-17.70964523992307
119	5	-18.188127768234196
119	6	-20.670969689613624
119	7	-20.30107337938694
119	8	-18.826146354377784
119	9	-20.003506119154903
119	10	-15.436574186539136
119	11	-21.078338744180304
119	12	-21.219122224747483
119	13	-21.731508932063303
119	14	-22.01754557395102
119	15	-20.10297536333922
119	16	-17.771311702876968
119	17	-18.30759776971624
119	18	-17.062492798369927
119	19	-14.352683751481191
119	20	-15.577131710592408
119	21	-11.490865490991258
119	22	-11.554014647211218
119	23	-11.711623108413287
119	24	-11.001086613905006
119	25	-10.48409653243786
119	26	-8.020089442915843
119	27	-6.931818285559233
119	28	-6.89044841924963
119	29	-6.234827033649003
119	30	-5.798124236160369
119	31	-5.277658477872068
119	32	-4.81593912898407
119	33	-5.045507102088042
119	34	-4.533726021259904
119	35	-4.13112152668487
119	36	-4.243246349433942
119	37	-4.163050841393373
119	38	-3.7949201177840175
119	39	-3.7633854292249325
119	40	-3.5699646882051503
120	1	2.1186498101075877
120	2	-8.20976461624402
120	3	12.339088271850208
120	4	-10.70964523992307
120	5	9.811872231765804
120	6	6.329030310386376
120	7	-16.30107337938694
120	8	8.173853645622216
120	9	-1.0035061191549026
120	10	11.563425813460864
120	11	-4.078338744180304
120	12	9.780877775252517
120	13	7.268491067936697
120	14	8.982454426048982
120	15	8.897024636660781
120	16	11.228688297123032
120	17	1.6924022302837614
120	18	13.937507201630073
120	19	16.64731624851881
120	20	-15.577131710592408
120	21	-11.490865490991258
120	22	-11.554014647211218
120	23	-11.711623108413287
120	24	-11.001086613905006
120	25	-10.48409653243786
120	26	-8.020089442915843
120	27	-6.931818285559233
120	28	-6.89044841924963
120	29	-6.234827033649003
120	30	-5.798124236160369
120	31	-5.277658477872068
120	32	-4.81593912898407
120	33	-5.045507102088042
120	34	-4.533726021259904
120	35	-4.13112152668487
120	36	-4.243246349433942
120	37	-4.163050841393373
120	38	-3.7949201177840175
120	39	-3.7633854292249325
120	40	-3.5699646882051503
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2494.0
5	329.0
6	251.0
7	429.0
8	241.0
9	277.0
10	550.0
11	741.0
12	892.0
13	970.0
14	1077.0
15	1197.0
16	1380.0
17	1376.0
18	1345.0
19	1225.0
20	1190.0
21	1052.0
22	1029.0
23	954.0
24	899.0
25	795.0
26	696.0
27	539.0
28	450.0
29	261.0
30	172.0
31	116.0
32	61.0
33	25.0
34	12.0
35	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.34793948878456	0.2347417840375587	0.28690662493479396	0.13041210224308816
8	97.9405630865485	0.4127563434132777	0.543100451859576	1.1035801181786584
9	97.13577885952712	1.57336578581363	0.6562934631432545	0.6345618915159944
10	45.63338689364659	24.80566291744474	19.854952881400095	9.705997307508577
11	37.68833311623464	28.21414615084017	18.145108766445226	15.952411966479962
12	38.153552197325	12.77575125933646	19.641306235886745	29.429390307451797
13	25.617645781772392	14.32851374234727	23.846120446354913	36.20772002952542
14	20.285701880074683	20.016499500673007	36.82428031783249	22.873518301419825
15	34.80960444618123	18.809430767226782	27.571534019365203	18.809430767226782
16	40.49324823064565	17.15079675220355	22.547870261821025	19.808084755329773
17	30.489340454170467	18.774694976336242	31.205766141287828	19.530198428205463
18	29.308323563892145	18.07129521080283	30.328687421301726	22.291693804003298
19	27.010417120690406	14.614479361896876	18.498016824303708	39.87708669310901
20	25.470018670487605	31.56614997177717	23.416265034084493	19.54756632365073
21	22.157092614302464	21.39290521471061	33.88910598758195	22.560896183404978
22	19.769006990577918	17.42868307932786	28.023099300942206	34.77921062915201
23	23.48573661586557	18.640093786635404	26.334071468889757	31.540098128609266
24	24.390985279430282	19.145425333275433	31.716531330062097	24.747058057232184
25	32.24418857267	18.366282859004997	22.454920703888767	26.934607864436238
26	20.339574876696783	17.93898127536991	27.061236960412028	34.66020688752128
27	18.276955143514698	15.992878544443961	26.996395848712496	38.733770463328845
28	18.104421857353834	23.455824863174353	25.010859178177398	33.42889410129442
29	30.386572161586294	17.180501804583205	24.781493238248466	27.651432795582032
30	22.65832138044943	14.725952970834962	36.07163037336463	26.544095275350983
31	23.50280520158309	21.354325229417647	30.27443134867133	24.868438220327928
32	34.0469883180614	13.636166239631736	27.411299778520863	24.90554566378599
33	23.6734693877551	14.867564046895353	29.63960052105949	31.819366044290053
34	17.39828926229864	20.333463592549172	36.689679128131644	25.578568017020537
35	15.840896261235832	19.544921620565376	40.93100004342351	23.68318207477528
36	16.17161716171617	24.53100573215216	30.284870592322388	29.012506513809278
37	18.618592528236316	18.653344917463073	37.645525629887054	25.08253692441355
38	24.663423955528533	16.802744723356206	27.9987839833232	30.535047337792058
39	20.86752637749121	16.807780817159482	28.457296687073946	33.867396118275366
40	18.5141765446572	16.655811732013373	36.79822847466458	28.031783248664844
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.5
10	2.0
11	2.5
12	3.0
13	3.0
14	10.0
15	17.0
16	26.5
17	36.0
18	36.0
19	59.0
20	82.0
21	119.0
22	156.0
23	156.0
24	174.5
25	193.0
26	219.0
27	245.0
28	245.0
29	265.5
30	286.0
31	346.5
32	407.0
33	407.0
34	490.0
35	573.0
36	630.0
37	687.0
38	687.0
39	767.5
40	848.0
41	906.5
42	965.0
43	965.0
44	1037.5
45	1110.0
46	1181.0
47	1252.0
48	1252.0
49	1486.0
50	1720.0
51	1841.5
52	1963.0
53	1963.0
54	2061.0
55	2159.0
56	2226.0
57	2293.0
58	2293.0
59	2302.5
60	2312.0
61	2221.5
62	2131.0
63	2131.0
64	1789.0
65	1447.0
66	1225.5
67	1004.0
68	1004.0
69	787.5
70	571.0
71	439.0
72	307.0
73	307.0
74	223.5
75	140.0
76	103.0
77	66.0
78	66.0
79	49.0
80	32.0
81	23.5
82	15.0
83	15.0
84	9.5
85	4.0
86	4.0
87	4.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.11723329425556857
8	0.06512960791976032
9	0.09986539881029916
10	0.01736789544526942
11	0.0
12	0.013025921583952065
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3820936997959272
20	0.0
21	0.0
22	0.0
23	0.0
24	0.00868394772263471
25	0.06947158178107768
26	0.5210368633580825
27	0.00868394772263471
28	0.039077764751856196
29	0.14762711128479006
30	0.10420737267161652
31	0.16499500673005948
32	0.01736789544526942
33	0.004341973861317355
34	0.0
35	0.00868394772263471
36	0.013025921583952065
37	0.0477617124744909
38	0.021709869306586773
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	23031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.64977638834614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.69735374632465	81.1080717294082
2	3.3509074318158776	5.740089444661543
3	0.907431815877522	2.3316399635274196
4	0.319375443577005	1.0941774130519732
5	0.15715299604582783	0.67300594850419
6	0.10645848119233499	0.5470887065259866
7	0.06590286930954072	0.39511962137987927
8	0.050694514853492856	0.34735790890538837
9	0.07604177228023928	0.5861664712778429
>10	0.23319476832606711	4.03803569102514
>50	0.025347257426746428	1.5500846684902958
>100	0.01013890297069857	1.5891624332421517
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	255	1.1072033346359256	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	111	0.48195909860622643	No Hit
TCACTTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	77	0.3343319873214363	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	76	0.32999001346011897	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	75	0.3256480395988016	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	75	0.3256480395988016	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	54	0.23446658851113714	No Hit
TCACTTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	46	0.1997307976205983	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT	43	0.18670487603664626	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	41	0.17802092831401156	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT	41	0.17802092831401156	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT	41	0.17802092831401156	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	33	0.1432851374234727	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	32	0.13894316356215536	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	32	0.13894316356215536	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	29	0.12591724197820328	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT	26	0.11289132039425122	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT	26	0.11289132039425122	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	25	0.10854934653293387	No Hit
TCACTTGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT	24	0.10420737267161652	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCCGTCGCCTCTGCT	24	0.10420737267161652	No Hit
TCACTTGGGAGTGCAGTGCTTCACTTGGGTCTCATAGCCT	24	0.10420737267161652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.004341973861317355	0.0	0.0	0.0	0.0
11	0.00868394772263471	0.0	0.0	0.0	0.0
12	0.00868394772263471	0.0	0.0	0.0	0.0
13	0.013025921583952065	0.0	0.0	0.0	0.0
14	0.01736789544526942	0.0	0.0	0.0	0.0
15	0.021709869306586773	0.0	0.0	0.0	0.0
16	0.02605184316790413	0.0	0.0	0.0	0.0
17	0.02605184316790413	0.0	0.0	0.0	0.0
18	0.02605184316790413	0.0	0.0	0.0	0.0
19	0.02605184316790413	0.0	0.0	0.0	0.0
20	0.030393817029221483	0.0	0.0	0.0	0.0
21	0.030393817029221483	0.0	0.0	0.0	0.0
22	0.030393817029221483	0.0	0.0	0.0	0.0
23	0.03473579089053884	0.0	0.0	0.0	0.0
24	0.043419738613173546	0.0	0.0	0.0	0.0
25	0.043419738613173546	0.0	0.0	0.0	0.0
26	0.043419738613173546	0.0	0.0	0.0	0.0
27	0.0477617124744909	0.0	0.0	0.0	0.0
28	0.05210368633580826	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCAGT	20	0.0026855106	34.120354	7
ACTTGGT	25	1.9263847E-4	34.120354	3
CCTGCCA	85	0.0	34.120354	22
CTCCACC	20	0.0026855106	34.120354	22
TTGGGTG	85	0.0	34.120354	5
CTTGGAA	20	0.0026855106	34.120354	4
TGGGGTA	20	0.0026855106	34.120354	6
CCCGTAG	40	7.2635885E-8	34.120354	26
TTGGGAT	100	0.0	34.120354	5
TTGGGAC	80	0.0	34.120354	5
GGGGGAA	25	1.9263847E-4	34.120354	7
GGGATGC	25	1.9263847E-4	34.120354	7
GGGACAG	20	0.0026855106	34.120354	7
TGGGTGT	40	7.2635885E-8	34.120354	6
TGGGTGG	25	1.9263847E-4	34.120354	6
TGGGCAA	20	0.0026855106	34.120354	6
GCCCGTA	40	7.2635885E-8	34.120354	25
GGGAAGT	45	5.2732503E-9	34.120354	7
CTTGAGA	20	0.0026855106	34.120354	4
TGGGATT	25	1.9263847E-4	34.120354	6
>>END_MODULE