FastQCFastQC Report
Fri 17 Jun 2016
SRR1525983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1011.903864278982092No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT981.8473138548539114No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG921.7342130065975494No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC541.0179076343072573No Hit
TCACTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT160.30160226201696516No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG150.2827521206409048No Hit
TCACTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG130.24505183788878418No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.22620169651272384No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.20735155513666353No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGTCACAGATCCTC110.20735155513666353No Hit
TCACTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT100.1885014137606032No Hit
TCACTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA100.1885014137606032No Hit
TCACTTGGGGAGTGCAGTGCTTCACTTGGGTCACAGATCC80.15080113100848258No Hit
TCACTTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT80.15080113100848258No Hit
TCACTTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG80.15080113100848258No Hit
TCACTTGGGATGTTACCTTATGCTGCTGGCTCTTGTACCT70.13195098963242224No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG70.13195098963242224No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT70.13195098963242224No Hit
TCACTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT70.13195098963242224No Hit
TCACTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT70.13195098963242224No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC70.13195098963242224No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC70.13195098963242224No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA70.13195098963242224No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.13195098963242224No Hit
TCACTTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT70.13195098963242224No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC60.11310084825636192No Hit
TCACTTGGGAGAGTTCTTTGTCATTACCTTTATGTTACTT60.11310084825636192No Hit
TCACTTGGGATTAGAAGAGGCCCTAAAACAAATTCAAGAG60.11310084825636192No Hit
TCACTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT60.11310084825636192No Hit
TCACTTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG60.11310084825636192No Hit
TCACTTGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG60.11310084825636192No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG60.11310084825636192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTT251.7695989E-434.00000417
CTCTTCT251.7695989E-434.00000419
TTGGGTA251.7695989E-434.0000045
CCTCTTC251.7695989E-434.00000418
TTGGGGC251.7695989E-434.0000045
TGGGGGC251.7695989E-434.0000046
TTGGGCT251.7695989E-434.0000045
TTGGGAT502.6921043E-1034.0000045
TGGGTCA251.7695989E-434.0000046
TCTTCTG251.7695989E-434.00000420
CTTCTGA251.7695989E-434.00000421
GGGTCAC200.002547102734.07
CTTGGGT800.034.04
CTTGGGC700.034.04
TTGGGTC453.958121E-934.05
TGGGGTG200.002547102734.06
TGGGGGA301.2252829E-534.06
TGGGGCT200.002547102734.06
TTGGGCA301.2252829E-534.05
TTGGGAC301.2252829E-534.05
Produced by FastQC (version 0.11.5)