##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5305
Sequences flagged as poor quality	0
Sequence length	40
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.116870876531575	33.0	30.0	34.0	4.0	36.0
2	18.237888784165882	20.0	4.0	30.0	4.0	35.0
3	16.77719132893497	16.0	4.0	28.0	4.0	33.0
4	16.404901036757774	14.0	4.0	28.0	4.0	34.0
5	16.025447690857682	14.0	4.0	27.0	4.0	33.0
6	15.71837888784166	14.0	4.0	26.0	4.0	33.0
7	17.663147973609803	17.0	4.0	31.0	4.0	35.0
8	17.286899151743636	17.0	4.0	30.0	4.0	34.0
9	16.372855796418474	9.0	4.0	31.0	4.0	34.0
10	14.402073515551367	4.0	4.0	28.0	4.0	33.0
11	12.530254476908576	4.0	4.0	23.0	4.0	33.0
12	12.155325164938738	4.0	4.0	23.0	4.0	31.0
13	11.095570216776625	4.0	4.0	18.0	4.0	30.0
14	11.52158341187559	4.0	4.0	20.0	4.0	31.0
15	11.213760603204523	4.0	4.0	19.0	4.0	31.0
16	9.65956644674835	4.0	4.0	14.0	4.0	28.0
17	9.241658812441093	4.0	4.0	12.0	4.0	26.0
18	8.812064090480678	4.0	4.0	10.0	4.0	25.0
19	8.617907634307258	4.0	4.0	10.0	4.0	24.0
20	9.155136663524976	4.0	4.0	10.0	4.0	27.0
21	8.25617342130066	4.0	4.0	7.0	4.0	23.0
22	8.141376060320452	4.0	4.0	7.0	4.0	23.0
23	8.349481621112158	4.0	4.0	5.0	4.0	24.0
24	8.026201696512723	4.0	4.0	4.0	4.0	22.0
25	8.4559849198869	4.0	4.0	4.0	4.0	25.0
26	8.51555136663525	4.0	4.0	4.0	4.0	26.0
27	8.689726672950048	4.0	4.0	4.0	4.0	27.0
28	8.89651272384543	4.0	4.0	4.0	4.0	27.0
29	8.077851083883129	4.0	4.0	4.0	4.0	24.0
30	8.436757775683317	4.0	4.0	4.0	4.0	26.0
31	9.251272384542885	4.0	4.0	4.0	4.0	30.0
32	9.186427898209237	4.0	4.0	4.0	4.0	30.0
33	9.001696512723845	4.0	4.0	4.0	4.0	30.0
34	9.194344957587182	4.0	4.0	4.0	4.0	31.0
35	9.06559849198869	4.0	4.0	4.0	4.0	30.0
36	9.156833176248822	4.0	4.0	4.0	4.0	32.0
37	8.94665409990575	4.0	4.0	4.0	4.0	31.0
38	8.761168708765316	4.0	4.0	4.0	4.0	31.0
39	8.575871819038642	4.0	4.0	4.0	4.0	30.0
40	8.607540056550425	4.0	4.0	4.0	4.0	30.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-13.495401936026925
1	2	-9.256840352934107
1	3	-9.832702395983642
1	4	-8.101231812169313
1	5	-2.751588804713805
1	6	-10.703273433742185
1	7	-12.234176211519962
1	8	-11.472136544011546
1	9	-10.952865710678209
1	10	-9.12946240680616
1	11	-7.933526109307358
1	12	-7.732408534752283
1	13	-6.160598544973546
1	14	-6.890407046657048
1	15	-6.760088233525737
1	16	-5.3918756012506
1	17	-4.825863546176047
1	18	-4.527350889850888
1	19	-4.341500946969699
1	20	-4.7064649470899464
1	21	-3.683116131553631
1	22	-3.715390512265511
1	23	-3.931153424122173
1	24	-3.339350724506975
1	25	-3.789697946729196
1	26	-3.6972676917989444
1	27	-3.8298648689273698
1	28	-4.396159887566139
1	29	-3.3507297679172696
1	30	-3.3848375871813374
1	31	-4.391915434102934
1	32	-4.202317445286196
1	33	-4.116769630832131
1	34	-4.4582995129870095
1	35	-4.481036180254931
1	36	-4.157467156685907
1	37	-4.270417718855219
1	38	-4.09923941798942
1	39	-4.093996136964888
1	40	-3.8890038780663794
2	1	9.254598063973074
2	2	14.659826313732559
2	3	16.00063093734969
2	4	13.565434854497353
2	5	10.748411195286195
2	6	7.296726566257815
2	7	9.09915712181337
2	8	6.611196789321786
2	9	-7.119532377344876
2	10	-0.9627957401394944
2	11	-4.766859442640691
2	12	1.9342581319143832
2	13	-0.07726521164021172
2	14	0.27625962000961835
2	15	3.156578433140929
2	16	-1.4752089345839332
2	17	2.5908031204906194
2	18	2.389315776815778
2	19	2.075165719696967
2	20	5.21020171957672
2	21	5.816883868446369
2	22	-0.29872384559884413
2	23	6.068846575877827
2	24	3.660649275493025
2	25	5.710302053270804
2	26	6.469398974867722
2	27	7.503468464405964
2	28	2.8538401124338613
2	29	5.982603565416064
2	30	2.9484957461519965
2	31	6.524751232563732
2	32	9.131015888047136
2	33	5.966563702501203
2	34	8.125033820346323
2	35	6.268963819745069
2	36	9.092532843314093
2	37	6.729582281144781
2	38	7.15076058201058
2	39	7.156003863035112
2	40	5.6943294552669546
3	1	5.504598063973074
3	2	4.493159647065891
3	3	6.917297604016358
3	4	2.0654348544973526
3	5	-2.168255471380471
3	6	1.4633932329244832
3	7	-2.4008428781866282
3	8	5.277863455988454
3	9	5.297134289321791
3	10	0.03720425986050557
3	11	3.8164738906926416
3	12	6.017591465247717
3	13	2.8394014550264544
3	14	-0.5570737133237138
3	15	2.073245099807597
3	16	4.774791065416066
3	17	4.924136453823953
3	18	3.639315776815778
3	19	4.158499053030301
3	20	5.8768683862433875
3	21	5.733550535113035
3	22	4.201276154401155
3	23	6.568846575877827
3	24	4.993982608826359
3	25	6.87696871993747
3	26	6.1360656415343895
3	27	8.42013513107263
3	28	8.02050677910053
3	29	6.14927023208273
3	30	7.4484957461519965
3	31	5.108084565897066
3	32	4.96434922138047
3	33	7.383230369167869
3	34	7.375033820346324
3	35	7.685630486411737
3	36	9.675866176647425
3	37	6.729582281144781
3	38	8.567427248677248
3	39	9.489337196368444
3	40	6.527662788600287
5	1	6.837931397306409
5	2	15.409826313732559
5	3	-0.9993690626503096
5	4	16.73210152116402
5	5	18.248411195286195
5	6	-1.2032734337421847
5	7	-8.234176211519962
5	8	-7.472136544011546
5	9	0.5471342893217912
5	10	2.8705375931938395
5	11	6.066473890692642
5	12	-5.232408534752283
5	13	3.3394014550264544
5	14	-3.8904070466570477
5	15	5.739911766474263
5	16	-2.3918756012506
5	17	4.174136453823953
5	18	0.4726491101491117
5	19	1.658499053030301
5	20	6.2935350529100536
5	21	-2.183116131553631
5	22	0.2846094877344889
5	23	7.568846575877827
5	24	-1.839350724506975
5	25	7.710302053270804
5	26	3.8027323082010556
5	27	-0.32986486892736977
5	28	7.103840112433861
5	29	-0.3507297679172696
5	30	0.6151624128186626
5	31	9.108084565897066
5	32	1.2976825547138038
5	33	9.383230369167869
5	34	9.04170048701299
5	35	2.0189638197450694
5	36	4.842532843314093
5	37	8.229582281144781
5	38	3.90076058201058
5	39	10.406003863035112
5	40	10.11099612193362
6	1	5.019749579124589
6	2	-4.226537322631078
6	3	-0.6811872444684912
6	4	1.1411924302549306
6	5	2.975683922558922
6	6	-6.521455251924003
6	7	-1.2341762115199622
6	8	5.164227092352091
6	9	6.592588834776338
6	10	-1.5840078613516155
6	11	1.7937466179653683
6	12	8.267591465247717
6	13	7.112128727753728
6	14	8.200502044252042
6	15	8.78536631192881
6	16	10.971760762385763
6	17	11.537772817460317
6	18	5.836285473785475
6	19	10.203953598484848
6	20	9.748080507455507
6	21	4.589611141173642
6	22	7.102791305916307
6	23	5.250664757696009
6	24	4.660649275493025
6	25	5.0284838714526225
6	26	5.484550490019237
6	27	6.0792260401635385
6	28	6.876567385161135
6	29	5.012906595719094
6	30	3.887889685545936
6	31	8.88081183862434
6	32	9.61586437289562
6	33	7.246866732804232
6	34	8.814427759740264
6	35	8.42805472883598
6	36	8.115260116041366
6	37	7.456855008417508
6	38	9.173487854737854
6	39	8.996912953944202
6	40	12.11099612193362
7	1	-14.16206860269359
7	2	-14.590173686267441
7	3	-11.49936906265031
7	4	-11.76789847883598
7	5	-11.751588804713805
7	6	-10.703273433742185
7	7	-12.234176211519962
7	8	-11.472136544011546
7	9	-10.952865710678209
7	10	-9.12946240680616
7	11	-7.933526109307358
7	12	-7.732408534752283
7	13	-6.160598544973546
7	14	-6.890407046657048
7	15	-6.760088233525737
7	16	-5.3918756012506
7	17	-4.825863546176047
7	18	-4.527350889850888
7	19	-4.341500946969699
7	20	-4.7064649470899464
7	21	-3.683116131553631
7	22	-3.715390512265511
7	23	-3.931153424122173
7	24	-3.339350724506975
7	25	-3.789697946729196
7	26	-3.6972676917989444
7	27	-3.8298648689273698
7	28	-4.396159887566139
7	29	-3.3507297679172696
7	30	-3.3848375871813374
7	31	-4.391915434102934
7	32	-4.202317445286196
7	33	-4.116769630832131
7	34	-4.4582995129870095
7	35	-4.481036180254931
7	36	-4.157467156685907
7	37	-4.270417718855219
7	38	-4.09923941798942
7	39	-4.093996136964888
7	40	-3.8890038780663794
8	1	-9.16206860269359
8	2	-14.590173686267441
8	3	-11.49936906265031
8	4	-11.76789847883598
8	5	-11.751588804713805
8	6	-10.703273433742185
8	7	-12.234176211519962
8	8	-11.472136544011546
8	9	-10.952865710678209
8	10	-9.12946240680616
8	11	-7.933526109307358
8	12	-7.732408534752283
8	13	-6.160598544973546
8	14	-6.890407046657048
8	15	-6.760088233525737
8	16	-5.3918756012506
8	17	-4.825863546176047
8	18	-4.527350889850888
8	19	-4.341500946969699
8	20	-4.7064649470899464
8	21	-3.683116131553631
8	22	-3.715390512265511
8	23	-3.931153424122173
8	24	-3.339350724506975
8	25	-3.789697946729196
8	26	-3.6972676917989444
8	27	-3.8298648689273698
8	28	-4.396159887566139
8	29	-3.3507297679172696
8	30	-3.3848375871813374
8	31	-4.391915434102934
8	32	-4.202317445286196
8	33	-4.116769630832131
8	34	-4.4582995129870095
8	35	-4.481036180254931
8	36	-4.157467156685907
8	37	-4.270417718855219
8	38	-4.09923941798942
8	39	-4.093996136964888
8	40	-3.8890038780663794
9	1	8.004598063973074
9	2	3.409826313732559
9	3	12.25063093734969
9	4	10.898768187830688
9	5	10.998411195286195
9	6	2.8800598995911475
9	7	8.59915712181337
9	8	-2.30546987734488
9	9	2.713800955988459
9	10	0.7872042598605056
9	11	0.8164738906926416
9	12	0.7675914652477172
9	13	0.8394014550264544
9	14	0.5262596200096183
9	15	0.906578433140929
9	16	1.4414577320827338
9	17	-2.3258635461760466
9	18	1.4726491101491117
9	19	-0.09150094696969902
9	20	-2.2064649470899464
9	21	1.983550535113035
9	22	1.451276154401155
9	23	1.235513242544493
9	24	4.577315942159691
9	25	4.710302053270804
9	26	2.6360656415343895
9	27	3.9201351310726302
9	28	3.3538401124338613
9	29	3.5659368987493965
9	30	6.615162412818663
9	31	4.9414178992304
9	32	4.047682554713804
9	33	3.799897035834535
9	34	3.3750338203463244
9	35	2.4356304864117355
9	36	5.1758661766474265
9	37	3.062915614478115
9	38	1.4007605820105802
9	39	4.989337196368446
9	40	1.7776627886002867
10	1	0.17126473063974146
10	2	-11.590173686267441
10	3	3.1672976040163583
10	4	-1.1012318121693134
10	5	-7.418255471380471
10	6	0.6300598995911493
10	7	11.765823788480038
10	8	11.527863455988454
10	9	16.04713428932179
10	10	13.203870926527172
10	11	23.399807224025977
10	12	23.26759146524772
10	13	25.17273478835979
10	14	18.442926286676283
10	15	23.239911766474265
10	16	9.6081243987494
10	17	1.8408031204906194
10	18	9.13931577681578
10	19	8.325165719696969
10	20	3.6268683862433875
10	21	-0.3497827982202981
10	22	1.951276154401155
10	23	-0.59782009078884
10	24	-0.6726840578403079
10	25	3.8769687199374703
10	26	0.9693989748677216
10	27	0.17013513107263023
10	28	3.937173445767195
10	29	0.6492702320827304
10	30	0.9484957461519965
10	31	5.608084565897066
10	32	4.797682554713804
10	33	3.883230369167869
10	34	6.2083671536796565
10	35	4.518963819745069
10	36	4.509199509980759
10	37	5.729582281144781
10	38	0.9007605820105802
10	39	5.572670529701778
10	40	3.1109961219336206
11	1	7.837931397306409
11	2	7.409826313732559
11	3	15.50063093734969
11	4	9.23210152116402
11	5	17.248411195286195
11	6	3.2967265662578153
11	7	13.765823788480038
11	8	14.527863455988454
11	9	12.047134289321791
11	10	16.870537593193838
11	11	4.066473890692642
11	12	13.267591465247717
11	13	7.839401455026454
11	14	13.109592953342952
11	15	9.239911766474263
11	16	1.6081243987493998
11	17	0.17413645382395337
11	18	2.4726491101491117
11	19	6.658499053030301
11	20	11.293535052910054
11	21	12.316883868446368
11	22	16.28460948773449
11	23	11.068846575877828
11	24	15.660649275493025
11	25	19.210302053270805
11	26	15.302732308201055
11	27	2.1701351310726302
11	28	18.60384011243386
11	29	11.649270232082731
11	30	22.615162412818663
11	31	22.608084565897066
11	32	12.797682554713804
11	33	11.883230369167869
11	34	22.54170048701299
11	35	22.518963819745068
11	36	2.8425328433140926
11	37	24.72958228114478
11	38	17.90076058201058
11	39	7.906003863035112
11	40	20.11099612193362
12	1	6.171264730639745
12	2	10.159826313732559
12	3	9.167297604016358
12	4	12.98210152116402
12	5	11.915077861952863
12	6	4.8800598995911475
12	7	16.015823788480038
12	8	11.777863455988454
12	9	13.130467622655123
12	10	11.537204259860507
12	11	7.4831405573593095
12	12	8.767591465247717
12	13	8.339401455026454
12	14	13.942926286676284
12	15	11.489911766474263
12	16	6.441457732082734
12	17	7.5074697871572855
12	18	5.805982443482446
12	19	8.575165719696969
12	20	8.960201719576721
12	21	10.1502172017797
12	22	7.867942821067823
12	23	6.902179909211161
12	24	9.743982608826357
12	25	9.793635386604137
12	26	9.802732308201055
12	27	9.753468464405962
12	28	9.02050677910053
12	29	10.0659368987494
12	30	8.78182907948533
12	31	4.9414178992304
12	32	4.047682554713804
12	33	3.383230369167869
12	34	6.9583671536796565
12	35	4.102297153078403
12	36	6.259199509980759
12	37	6.062915614478115
12	38	2.5674272486772463
12	39	4.406003863035112
12	40	6.1943294552669546
14	1	0.43793139730641073
14	2	2.2098263137325596
14	3	3.7006309373496897
14	4	3.832101521164022
14	5	-1.9515888047138041
14	6	-6.303273433742184
14	7	-5.434176211519961
14	8	-1.472136544011546
14	9	-9.752865710678208
14	10	-4.72946240680616
14	11	-6.1335261093073585
14	12	-6.532408534752283
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112	1	-1.7620686026935921
112	2	-14.590173686267441
112	3	-11.49936906265031
112	4	-11.76789847883598
112	5	-11.751588804713805
112	6	-10.703273433742185
112	7	-12.234176211519962
112	8	-11.472136544011546
112	9	-10.952865710678209
112	10	-9.12946240680616
112	11	-7.933526109307358
112	12	-7.732408534752283
112	13	-6.160598544973546
112	14	-6.890407046657048
112	15	-6.760088233525737
112	16	-5.3918756012506
112	17	-4.825863546176047
112	18	-4.527350889850888
112	19	-4.341500946969699
112	20	-4.7064649470899464
112	21	-3.683116131553631
112	22	-3.715390512265511
112	23	-3.931153424122173
112	24	-3.339350724506975
112	25	-3.789697946729196
112	26	-3.6972676917989444
112	27	-3.8298648689273698
112	28	-4.396159887566139
112	29	-3.3507297679172696
112	30	-3.3848375871813374
112	31	-4.391915434102934
112	32	-4.202317445286196
112	33	-4.116769630832131
112	34	-4.4582995129870095
112	35	-4.481036180254931
112	36	-4.157467156685907
112	37	-4.270417718855219
112	38	-4.09923941798942
112	39	-4.093996136964888
112	40	-3.8890038780663794
114	1	6.837931397306409
114	2	13.409826313732559
114	3	3.5006309373496904
114	4	3.2321015211640205
114	5	18.248411195286195
114	6	5.296726566257815
114	7	14.765823788480038
114	8	-11.472136544011546
114	9	-10.952865710678209
114	10	-9.12946240680616
114	11	-7.933526109307358
114	12	-7.732408534752283
114	13	-6.160598544973546
114	14	-6.890407046657048
114	15	-6.760088233525737
114	16	-5.3918756012506
114	17	-4.825863546176047
114	18	-4.527350889850888
114	19	-4.341500946969699
114	20	-4.7064649470899464
114	21	-3.683116131553631
114	22	-3.715390512265511
114	23	-3.931153424122173
114	24	-3.339350724506975
114	25	-3.789697946729196
114	26	-3.6972676917989444
114	27	-3.8298648689273698
114	28	-4.396159887566139
114	29	-3.3507297679172696
114	30	-3.3848375871813374
114	31	-4.391915434102934
114	32	-4.202317445286196
114	33	-4.116769630832131
114	34	-4.4582995129870095
114	35	-4.481036180254931
114	36	-4.157467156685907
114	37	-4.270417718855219
114	38	-4.09923941798942
114	39	-4.093996136964888
114	40	-3.8890038780663794
117	1	4.337931397306409
117	2	5.909826313732559
117	3	10.00063093734969
117	4	-5.2678984788359795
117	5	-1.2515888047138048
117	6	-0.7032734337421847
117	7	-4.234176211519962
117	8	2.527863455988454
117	9	3.047134289321791
117	10	-9.12946240680616
117	11	-7.933526109307358
117	12	-7.732408534752283
117	13	-6.160598544973546
117	14	-6.890407046657048
117	15	-6.760088233525737
117	16	-5.3918756012506
117	17	-4.825863546176047
117	18	-4.527350889850888
117	19	-4.341500946969699
117	20	-4.7064649470899464
117	21	-3.683116131553631
117	22	-3.715390512265511
117	23	-3.931153424122173
117	24	-3.339350724506975
117	25	-3.789697946729196
117	26	-3.6972676917989444
117	27	-3.8298648689273698
117	28	-4.396159887566139
117	29	-3.3507297679172696
117	30	-3.3848375871813374
117	31	-4.391915434102934
117	32	-4.202317445286196
117	33	-4.116769630832131
117	34	-4.4582995129870095
117	35	-4.481036180254931
117	36	-4.157467156685907
117	37	-4.270417718855219
117	38	-4.09923941798942
117	39	-4.093996136964888
117	40	-3.8890038780663794
119	1	5.837931397306409
119	2	13.076492980399227
119	3	-2.1660357293169756
119	4	4.2321015211640205
119	5	6.581744528619527
119	6	8.630059899591147
119	7	0.09915712181337
119	8	6.194530122655122
119	9	7.713800955988459
119	10	3.5372042598605073
119	11	3.0664738906926416
119	12	11.267591465247717
119	13	-4.160598544973546
119	14	-4.890407046657048
119	15	-4.760088233525737
119	16	-4.3918756012506
119	17	0.5074697871572873
119	18	1.4726491101491117
119	19	-0.00816761363636509
119	20	0.6268683862433875
119	21	-2.016449464886964
119	22	-2.048723845598844
119	23	-2.931153424122173
119	24	1.660649275493025
119	25	-2.123031280062529
119	26	1.6360656415343895
119	27	1.1701351310726302
119	28	1.9371734457671952
119	29	2.6492702320827304
119	30	1.9484957461519965
119	31	6.9414178992304
119	32	7.131015888047138
119	33	7.216563702501203
119	34	5.2083671536796565
119	35	6.852297153078403
119	36	-4.157467156685907
119	37	-4.270417718855219
119	38	-4.09923941798942
119	39	-4.093996136964888
119	40	-3.8890038780663794
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1939.0
5	134.0
6	135.0
7	201.0
8	234.0
9	328.0
10	281.0
11	242.0
12	140.0
13	128.0
14	105.0
15	74.0
16	51.0
17	54.0
18	49.0
19	56.0
20	94.0
21	139.0
22	185.0
23	193.0
24	145.0
25	119.0
26	100.0
27	67.0
28	50.0
29	37.0
30	12.0
31	8.0
32	4.0
33	0.0
34	0.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.24542539143559	0.32069420863987924	0.3018298434257687	0.1320505564987738
8	99.0192380233874	0.4526593738211996	0.3206337231233497	0.2074688796680498
9	97.46691871455576	0.4914933837429111	1.2098298676748582	0.831758034026465
10	41.35395059400339	28.267018668678105	19.290967376956438	11.088063360362058
11	28.97266729500471	25.070688030160227	22.921771913289348	23.03487276154571
12	30.48642533936652	25.678733031674206	21.19155354449472	22.643288084464555
13	19.66408756369126	21.79656538969617	24.098886582374032	34.440460464238534
14	20.252687158212332	34.18819536111635	28.323590420516688	17.235527060154627
15	37.28557964184731	23.58152686145146	22.657869934024504	16.475023562676718
16	29.29311969839774	33.0631479736098	23.54382657869934	14.099905749293121
17	26.4894419306184	22.963800904977376	36.29336349924585	14.25339366515837
18	23.675777568331764	21.526861451460885	27.426955702167767	27.370405278039584
19	23.30188679245283	25.71698113207547	25.90566037735849	25.075471698113205
20	22.469368520263902	27.181903864278983	33.628652214891616	16.720075400565506
21	20.56550424128181	24.297832233741754	29.029217719132895	26.107445805843543
22	18.30348727615457	21.16870876531574	37.342130065975496	23.185673892554195
23	19.13650075414781	25.282805429864254	33.1447963800905	22.435897435897438
24	19.943449575871817	26.55984919886899	28.82186616399623	24.67483506126296
25	23.576009053187477	25.235760090531873	30.761976612599017	20.42625424368163
26	31.180358829084042	26.74220963172805	22.379603399433428	19.697828139754485
27	20.726172465960666	32.90468986384266	23.07110438729198	23.29803328290469
28	24.768911526127148	34.72929635917751	22.184493491794	18.31729862290134
29	31.66855845629966	29.37949300037836	22.17177449867575	16.780174044646234
30	26.518294983025275	25.820445115050923	30.101848359109766	17.559411542814033
31	27.19215538374505	32.095040543088814	24.212709786913067	16.500094286253063
32	29.471890971039183	28.809388604959302	24.11508612530759	17.603634298693922
33	27.992459943449578	28.859566446748353	23.675777568331764	19.472196041470312
34	19.773798303487276	28.72761545711593	35.96606974552309	15.532516493873704
35	18.793590951932142	27.935909519321395	37.39868049010367	15.87181903864279
36	22.425845456262987	31.3054978273191	28.773852257698845	17.494804458719063
37	24.811463046757165	27.37556561085973	31.14630467571644	16.666666666666664
38	26.37134778510839	27.822808671065037	27.257304429783225	18.548539114043354
39	24.863336475023562	27.257304429783225	22.921771913289348	24.957587181903865
40	22.417043740573153	25.16968325791855	32.22096530920061	20.192307692307693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	9.5
12	19.0
13	19.0
14	20.5
15	22.0
16	26.5
17	31.0
18	31.0
19	35.0
20	39.0
21	37.5
22	36.0
23	36.0
24	38.0
25	40.0
26	49.5
27	59.0
28	59.0
29	89.0
30	119.0
31	124.5
32	130.0
33	130.0
34	151.5
35	173.0
36	209.0
37	245.0
38	245.0
39	301.5
40	358.0
41	424.5
42	491.0
43	491.0
44	458.0
45	425.0
46	434.5
47	444.0
48	444.0
49	515.5
50	587.0
51	594.5
52	602.0
53	602.0
54	539.5
55	477.0
56	446.5
57	416.0
58	416.0
59	372.0
60	328.0
61	235.5
62	143.0
63	143.0
64	109.0
65	75.0
66	48.5
67	22.0
68	22.0
69	15.5
70	9.0
71	8.0
72	7.0
73	7.0
74	6.5
75	6.0
76	4.0
77	2.0
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.07540056550424129
8	0.05655042412818096
9	0.2827521206409048
10	0.037700282752120645
11	0.0
12	0.018850141376060323
13	0.11310084825636192
14	0.037700282752120645
15	0.0
16	0.0
17	0.018850141376060323
18	0.0
19	0.0942507068803016
20	0.0
21	0.0
22	0.0
23	0.018850141376060323
24	0.0
25	0.05655042412818096
26	0.1885014137606032
27	0.32045240339302544
28	0.07540056550424129
29	0.35815268614514606
30	0.05655042412818096
31	0.037700282752120645
32	0.41470311027332707
33	0.0
34	0.0
35	0.0
36	0.22620169651272384
37	0.018850141376060323
38	0.0
39	0.0
40	0.018850141376060323
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	5305.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.20546654099905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.75395551479019	76.248821866164
2	4.677826186654437	7.690857681432611
3	1.3070396698005045	3.223374175306315
4	0.2980967667966063	0.9802073515551367
5	0.2293052052281587	0.9425070688030159
6	0.16051364365971107	0.7917059377945336
7	0.2293052052281587	1.3195098963242224
8	0.06879156156844761	0.4524033930254477
9	0.0	0.0
>10	0.18344416418252693	1.8473138548539114
>50	0.06879156156844761	4.599434495758718
>100	0.022930520522815866	1.903864278982092
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	101	1.903864278982092	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	98	1.8473138548539114	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	92	1.7342130065975494	No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	54	1.0179076343072573	No Hit
TCACTTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	16	0.30160226201696516	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	15	0.2827521206409048	No Hit
TCACTTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	13	0.24505183788878418	No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12	0.22620169651272384	No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.20735155513666353	No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGTCACAGATCCTC	11	0.20735155513666353	No Hit
TCACTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	10	0.1885014137606032	No Hit
TCACTTGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA	10	0.1885014137606032	No Hit
TCACTTGGGGAGTGCAGTGCTTCACTTGGGTCACAGATCC	8	0.15080113100848258	No Hit
TCACTTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT	8	0.15080113100848258	No Hit
TCACTTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG	8	0.15080113100848258	No Hit
TCACTTGGGATGTTACCTTATGCTGCTGGCTCTTGTACCT	7	0.13195098963242224	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	7	0.13195098963242224	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	7	0.13195098963242224	No Hit
TCACTTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT	7	0.13195098963242224	No Hit
TCACTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	7	0.13195098963242224	No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC	7	0.13195098963242224	No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	7	0.13195098963242224	No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	7	0.13195098963242224	No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.13195098963242224	No Hit
TCACTTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT	7	0.13195098963242224	No Hit
TCACTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	6	0.11310084825636192	No Hit
TCACTTGGGAGAGTTCTTTGTCATTACCTTTATGTTACTT	6	0.11310084825636192	No Hit
TCACTTGGGATTAGAAGAGGCCCTAAAACAAATTCAAGAG	6	0.11310084825636192	No Hit
TCACTTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	6	0.11310084825636192	No Hit
TCACTTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG	6	0.11310084825636192	No Hit
TCACTTGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG	6	0.11310084825636192	No Hit
TCACTTGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG	6	0.11310084825636192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.01885014137606032	0.0	0.0	0.0	0.0
11	0.01885014137606032	0.0	0.0	0.0	0.0
12	0.01885014137606032	0.0	0.0	0.0	0.0
13	0.03770028275212064	0.0	0.0	0.0	0.0
14	0.07540056550424128	0.0	0.0	0.0	0.0
15	0.07540056550424128	0.0	0.0	0.0	0.0
16	0.07540056550424128	0.0	0.0	0.0	0.0
17	0.07540056550424128	0.0	0.0	0.0	0.0
18	0.07540056550424128	0.0	0.0	0.0	0.0
19	0.07540056550424128	0.0	0.0	0.0	0.0
20	0.07540056550424128	0.0	0.0	0.0	0.0
21	0.07540056550424128	0.0	0.0	0.0	0.0
22	0.07540056550424128	0.0	0.0	0.0	0.0
23	0.07540056550424128	0.0	0.0	0.0	0.0
24	0.07540056550424128	0.0	0.0	0.0	0.0
25	0.07540056550424128	0.0	0.0	0.0	0.0
26	0.07540056550424128	0.0	0.0	0.0	0.0
27	0.07540056550424128	0.0	0.0	0.0	0.0
28	0.07540056550424128	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCTT	25	1.7695989E-4	34.000004	17
CTCTTCT	25	1.7695989E-4	34.000004	19
TTGGGTA	25	1.7695989E-4	34.000004	5
CCTCTTC	25	1.7695989E-4	34.000004	18
TTGGGGC	25	1.7695989E-4	34.000004	5
TGGGGGC	25	1.7695989E-4	34.000004	6
TTGGGCT	25	1.7695989E-4	34.000004	5
TTGGGAT	50	2.6921043E-10	34.000004	5
TGGGTCA	25	1.7695989E-4	34.000004	6
TCTTCTG	25	1.7695989E-4	34.000004	20
CTTCTGA	25	1.7695989E-4	34.000004	21
GGGTCAC	20	0.0025471027	34.0	7
CTTGGGT	80	0.0	34.0	4
CTTGGGC	70	0.0	34.0	4
TTGGGTC	45	3.958121E-9	34.0	5
TGGGGTG	20	0.0025471027	34.0	6
TGGGGGA	30	1.2252829E-5	34.0	6
TGGGGCT	20	0.0025471027	34.0	6
TTGGGCA	30	1.2252829E-5	34.0	5
TTGGGAC	30	1.2252829E-5	34.0	5
>>END_MODULE