FastQCFastQC Report
Fri 17 Jun 2016
SRR1525982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15354
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3342.175328904519995No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3132.038556727888498No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1410.9183274716686205No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC850.5536016673179628No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT740.48195909860622643No Hit
ACGAGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT650.4233424514784421No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC380.24749251009508924No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG370.24097954930311322No Hit
ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC370.24097954930311322No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT320.20841474534323304Illumina Single End Adapter 2 (95% over 24bp)
ACGAGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA300.19538882375928096No Hit
ACGAGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC290.18887586296730494No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT280.1823629021753289No Hit
ACGAGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC270.1758499413833529No Hit
ACGAGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC250.1628240197994008No Hit
ACGAGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG240.15631105900742479No Hit
ACGAGCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG240.15631105900742479No Hit
ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC220.1432851374234727No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT220.1432851374234727Illumina Single End Adapter 1 (95% over 23bp)
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA210.13677217663149666No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT210.13677217663149666No Hit
ACGAGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT200.13025921583952063No Hit
ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC200.13025921583952063No Hit
ACGAGCGGGATCCAAAGGGCTACTCTGGAGCCATTGCCTGATCCATCAGTGGTTAA190.12374625504754462No Hit
ACGAGCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC190.12374625504754462No Hit
ACGAGCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG170.11072033346359254No Hit
ACGAGCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC170.11072033346359254No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG160.10420737267161652No Hit
ACGAGCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC160.10420737267161652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC203.7505373E-450.7941
TCACTCC150.007773753350.7941
TTTTGTC309.2101436E-750.45364435
TTCACTC150.007981691550.45364440
TAGCGCT150.007981691550.45364437
TCTAGCG150.007981691550.45364435
CGCTAAT150.007981691550.45364440
ATCAGTG150.007981691550.45364445
GCGCTAA150.007981691550.45364439
CTAGCGC150.007981691550.45364436
TTGTCCA203.8764044E-450.4536437
TCCAATC203.8764044E-450.4536440
TTTGTCC203.8764044E-450.4536436
TGGTTAA150.00808719850.28713250
GCCCAAA150.00808719850.28713248
ACTCCGC150.00808719850.28713243
CATCAGT150.00808719850.28713244
TTGGGCC150.00808719850.28713234
CCCAAAA150.00808719850.28713249
CCATCAG150.00808719850.28713243
Produced by FastQC (version 0.11.5)