##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525982_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15354 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.15207763449264 38.0 36.0 39.0 32.0 40.0 2 35.19825452650775 38.0 35.0 39.0 25.0 40.0 3 34.57066562459294 38.0 35.0 39.0 23.0 40.0 4 35.21199687377882 38.0 35.0 39.0 28.0 40.0 5 33.69506317571968 38.0 33.0 39.0 20.0 40.0 6 34.4347401328644 38.0 35.0 39.0 23.0 40.0 7 34.962876123485735 38.0 35.0 39.0 27.0 40.0 8 35.08186791715514 38.0 35.0 39.0 27.0 40.0 9 35.20704702357692 38.0 35.0 39.0 27.0 40.0 10 33.931809300508014 38.0 34.0 39.0 22.0 40.0 11 33.859906213364596 38.0 33.0 39.0 24.0 40.0 12 33.245668881073335 37.0 32.0 39.0 20.0 40.0 13 32.5230558812036 36.0 31.0 39.0 16.0 40.0 14 33.62205288524163 38.0 33.0 39.0 22.0 40.0 15 31.860296991012113 36.0 30.0 39.0 13.0 40.0 16 31.89064738830272 36.0 29.0 38.0 17.0 40.0 17 30.63872606486909 35.0 27.0 38.0 12.0 40.0 18 30.354630715123093 35.0 26.0 38.0 12.0 40.0 19 31.55757457340107 36.0 29.0 38.0 16.0 40.0 20 31.80395988016152 36.0 29.0 38.0 18.0 40.0 21 32.40061221831444 37.0 30.0 39.0 17.0 40.0 22 31.753484434023708 36.0 29.0 39.0 15.0 40.0 23 32.511853588641394 37.0 31.0 39.0 17.0 40.0 24 31.734531718119058 36.0 29.0 39.0 14.0 40.0 25 32.184186531197085 36.0 30.0 39.0 17.0 40.0 26 32.32486648430376 36.0 31.0 39.0 16.0 40.0 27 32.408883678520255 36.0 31.0 39.0 17.0 40.0 28 32.51348182883939 37.0 31.0 39.0 17.0 40.0 29 32.02917806434805 36.0 31.0 39.0 14.0 40.0 30 31.70392080239677 36.0 30.0 39.0 12.0 40.0 31 32.20177152533542 36.0 31.0 39.0 13.0 40.0 32 32.15663670704702 36.0 31.0 39.0 11.0 40.0 33 31.830858408232384 36.0 31.0 38.0 10.0 39.0 34 31.517454734922495 36.0 30.0 38.0 8.0 39.0 35 31.60101602188355 36.0 31.0 38.0 4.0 40.0 36 30.576787807737396 35.0 29.0 38.0 4.0 39.0 37 30.398788589292693 35.0 29.0 38.0 4.0 39.0 38 29.811319525856455 35.0 28.0 38.0 4.0 39.0 39 28.653054578611435 34.0 25.0 38.0 4.0 39.0 40 26.35951543571708 33.0 15.0 37.0 4.0 39.0 41 24.17187703530025 29.0 9.0 36.0 4.0 39.0 42 24.856584603360687 30.0 13.0 35.0 4.0 39.0 43 25.196561156701836 30.0 12.0 36.0 4.0 39.0 44 24.55978898007034 30.0 10.0 36.0 4.0 39.0 45 25.00234466588511 30.0 10.0 36.0 4.0 39.0 46 23.07027484694542 28.0 4.0 35.0 4.0 38.0 47 22.438517650123746 27.0 4.0 35.0 4.0 38.0 48 22.67409144196952 27.0 4.0 35.0 4.0 38.0 49 21.960205809561028 26.0 4.0 34.0 4.0 38.0 50 22.54656766966263 27.0 4.0 35.0 4.0 38.0 51 22.685033216100038 28.0 4.0 35.0 4.0 38.0 52 22.412465806955844 28.0 4.0 35.0 4.0 38.0 53 22.209977855933307 28.0 4.0 35.0 4.0 38.0 54 21.8421258304025 27.0 4.0 34.0 4.0 38.0 55 21.890582258694803 28.0 4.0 35.0 4.0 38.0 56 21.04324605965872 26.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.181527063288179 1 2 5.1471057523461425 1 3 5.916656474878742 1 4 4.369571419980836 1 5 6.534200611073484 1 6 5.434133062236057 1 7 4.988901583925099 1 8 4.825486922846586 1 9 5.160049535907291 1 10 5.3838323864727435 1 11 0.6697035557064694 1 12 -4.058136175386657 1 13 -16.899680452609836 1 14 -2.917536240057384 1 15 1.9047995997960143 1 16 0.6632649708219454 1 17 -3.153573120998633 1 18 -3.838721339466165 1 19 1.2916842565587388 1 20 0.9486932357807554 1 21 5.914688548923408 1 22 7.01478751417995 1 23 6.627672394065662 1 24 7.041819466555591 1 25 6.272553278191772 1 26 5.496643597014106 1 27 4.576798905737487 1 28 5.530859938801264 1 29 5.549457936968537 1 30 6.576160259616582 1 31 4.9651689332673286 1 32 4.428629809662393 1 33 3.984229833643056 1 34 4.182366567911707 1 35 5.194362831376196 1 36 4.529700477907767 1 37 3.7662902678615104 1 38 4.59982527893159 1 39 5.696926813062834 1 40 -7.943018741466082 1 41 2.399333898079142 1 42 1.441942305998424 1 43 -8.054344289339118 1 44 -13.019835698210363 1 45 -0.9634028874537179 1 46 -1.5572357698432668 1 47 -5.884954010633054 1 48 -1.8277090223924013 1 49 -11.982089826441737 1 50 -1.6451654746941813 1 51 5.802116554257719 1 52 5.70130914556886 1 53 6.101959882448728 1 54 6.797846871646879 1 55 5.527625480894379 1 56 8.144351264956924 2 1 4.277486659247778 2 2 4.675725280965672 2 3 6.116993175215441 2 4 4.876305426714843 2 5 6.636894213767082 2 6 5.10921723732023 2 7 5.197655792679306 2 8 4.960167057526718 2 9 4.951295327153083 2 10 4.723899726540079 2 11 3.59226247826539 2 12 -0.08507220232268509 2 13 1.8376932847639011 2 14 4.819837497316357 2 15 4.066415761412173 2 16 4.27437608193306 2 17 7.282453815028301 2 18 3.1191911184462953 2 19 7.050943515817998 2 20 3.941959229046745 2 21 6.581355215590072 2 22 6.844753844146275 2 23 6.006460272853545 2 24 6.743839668575799 2 25 5.440903446541938 2 26 4.348495448865961 2 27 5.682859511798096 2 28 5.636920544861873 2 29 5.278414165924765 2 30 6.495352178808506 2 31 4.094798562896955 2 32 5.596979978012559 2 33 3.7317045811178033 2 34 4.732871618416759 2 35 4.793689430702791 2 36 5.541484989692279 2 37 5.835313836885081 2 38 5.776592955699265 2 39 7.191876308012329 2 40 5.296038497591159 2 41 9.022229520974765 2 42 6.2230870871432025 2 43 6.780672545677717 2 44 -0.7437414221160843 2 45 6.376664452613621 2 46 6.813134600527103 2 47 3.613362487683446 2 48 5.8709441762607995 2 49 0.6778428334909243 2 50 6.5383362088075 2 51 10.967099719240881 2 52 10.357874802134518 2 53 10.514417794906642 2 54 9.333200407000412 2 55 9.775100228369126 2 56 10.343004463610125 3 1 4.419708881470001 3 2 5.681651206891594 3 3 6.558474656696923 3 4 4.985935056344466 3 5 6.888746065618935 3 6 6.039587607690606 3 7 5.436174311197824 3 8 4.992759650119311 3 9 4.912776808634561 3 10 5.180196022836373 3 11 5.81152173752465 3 12 5.716409279158796 3 13 6.562137729208345 3 14 6.184281941760801 3 15 0.5375268725232871 3 16 7.563264970821944 3 17 8.080972333546821 3 18 8.49400593326111 3 19 8.07168425655874 3 20 6.712329599417117 3 21 7.274688548923407 3 22 7.616605695998132 3 23 7.753126939520207 3 24 8.272728557464685 3 25 8.021644187282682 3 26 6.856643597014106 3 27 6.789526178464758 3 28 6.263587211528538 3 29 6.3258215733321705 3 30 6.979796623252948 3 31 6.225168933267327 3 32 7.097720718753298 3 33 5.380593470006687 3 34 6.275093840638981 3 35 7.270726467739827 3 36 8.233336841544137 3 37 8.259017540588783 3 38 8.634370733477041 3 39 9.049654085790106 3 40 11.067890349443012 3 41 15.002970261715507 3 42 11.58012412418024 3 43 13.974746619751787 3 44 14.071073392698729 3 45 12.31296074890992 3 46 14.56276423015673 3 47 14.565955080276037 3 48 15.465018250334875 3 49 12.261546537194626 3 50 13.18574361621491 3 51 12.773025645166811 3 52 11.366763691023408 3 53 12.849232609721458 3 54 10.32875596255597 3 55 12.183989117258015 3 56 11.017078537684199 4 1 4.419708881470001 4 2 5.961651206891595 4 3 6.918474656696922 4 4 5.46593505634447 4 5 7.648746065618933 4 6 6.8395876076906035 4 7 5.556174311197829 4 8 5.232759650119313 4 9 5.592776808634561 4 10 5.620196022836378 4 11 5.731521737524652 4 12 6.476409279158794 4 13 6.522137729208346 4 14 5.704281941760797 4 15 6.897526872523287 4 16 7.2032649708219445 4 17 9.80097233354682 4 18 9.294005933261108 4 19 8.591684256558736 4 20 7.832329599417122 4 21 7.594688548923408 4 22 8.216605695998133 4 23 7.95312693952021 4 24 8.272728557464685 4 25 7.821644187282679 4 26 6.216643597014105 4 27 5.909526178464763 4 28 7.583587211528538 4 29 7.125821573332168 4 30 6.499796623252951 4 31 6.585168933267326 4 32 6.377720718753299 4 33 6.860593470006691 4 34 7.035093840638979 4 35 7.79072646773983 4 36 7.3533368415441345 4 37 8.419017540588786 4 38 9.154370733477045 4 39 10.809654085790111 4 40 2.3478903494430092 4 41 14.922970261715502 4 42 12.420124124180237 4 43 12.574746619751789 4 44 13.391073392698729 4 45 12.432960748909917 4 46 15.402764230156734 4 47 16.44595508027604 4 48 15.865018250334874 4 49 14.26154653719463 4 50 16.86574361621491 4 51 14.81302564516681 4 52 14.926763691023407 4 53 14.289232609721456 4 54 14.328755962555967 4 55 14.303989117258016 4 56 13.6570785376842 5 1 4.573782955544075 5 2 5.675725280965672 5 3 6.376252434474701 5 4 5.4688980193074315 5 5 7.266523843396715 5 6 5.664772792875787 5 7 5.012470607494123 5 8 5.10831520567487 5 9 5.284628660486419 5 10 6.4646404672808195 5 11 5.036706922709833 5 12 5.137150019899536 5 13 7.689545136615749 5 14 6.782800460279319 5 15 8.066415761412173 5 16 7.385487193044167 5 17 9.06023159280608 5 18 9.304376303631482 5 19 7.458350923225403 5 20 6.830848117935638 5 21 8.507281141516003 5 22 8.548457547849985 5 23 6.969423235816507 5 24 8.299395224131349 5 25 6.552014557653052 5 26 5.533680634051144 5 27 5.127303956242539 5 28 6.081364989306316 5 29 6.4265623140729105 5 30 7.569426252882575 5 31 4.205909674008069 5 32 5.3377207187533 5 33 6.546519395932613 5 34 5.881019766564904 5 35 4.31220794922131 5 36 6.393336841544134 5 37 7.3538323554036005 5 38 7.961778140884448 5 39 9.599283715419734 5 40 10.703445904998567 5 41 14.022229520974765 5 42 12.852716716772836 5 43 11.891783656788828 5 44 13.478480800106134 5 45 12.228516304465476 5 46 14.07239385978636 5 47 13.242992117313076 5 48 13.426499731816353 5 49 14.344509500157589 5 50 14.464262134733428 5 51 14.37450712664829 5 52 14.209726653986369 5 53 13.4773807578696 5 54 14.62949670329671 5 55 13.886211339480237 5 56 14.454115574721236 6 1 3.45970888147 6 2 4.001651206891594 6 3 5.118474656696925 6 4 3.865935056344469 6 5 4.808746065618937 6 6 3.719587607690606 6 7 3.4761743111978234 6 8 3.35275965011931 6 9 4.152776808634563 6 10 4.380196022836376 6 11 5.691521737524653 6 12 4.956409279158798 6 13 5.442137729208341 6 14 5.2642819417607996 6 15 6.897526872523287 6 16 6.883264970821944 6 17 6.8809723335468185 6 18 7.014005933261107 6 19 7.111684256558739 6 20 6.5123295994171215 6 21 6.354688548923406 6 22 7.496605695998127 6 23 5.833126939520206 6 24 5.192728557464687 6 25 6.6616441872826755 6 26 4.656643597014103 6 27 2.1095261784647583 6 28 4.6235872115285375 6 29 4.405821573332169 6 30 4.7797966232529525 6 31 4.545168933267327 6 32 4.6577207187533 6 33 3.9405934700066894 6 34 1.7950938406389803 6 35 3.750726467739831 6 36 4.713336841544134 6 37 4.139017540588785 6 38 6.594370733477042 6 39 6.52965408579011 6 40 2.7078903494430087 6 41 11.002970261715507 6 42 7.700124124180242 6 43 8.614746619751791 6 44 9.751073392698729 6 45 10.112960748909916 6 46 11.122764230156733 6 47 12.44595508027604 6 48 10.705018250334874 6 49 10.981546537194628 6 50 12.86574361621491 6 51 11.093025645166808 6 52 10.406763691023407 6 53 11.689232609721454 6 54 11.32875596255597 6 55 9.743989117258014 6 56 10.057078537684198 7 1 4.499708881469999 7 2 5.688607728630728 7 3 6.7289094393056175 7 4 4.375500273735774 7 5 7.271354761271109 7 6 6.566544129429737 7 7 4.8900873546760835 7 8 4.726672693597571 7 9 5.3892985477650015 7 10 6.672369935879853 7 11 6.242826085350735 7 12 7.135539713941405 7 13 7.803876859643125 7 14 7.003412376543409 7 15 8.655787742088506 7 16 8.03283018821325 7 17 9.148798420503343 7 18 10.45574506369589 7 19 8.182988604384825 7 20 8.660155686373642 7 21 8.827732027184275 7 22 8.261823087302481 7 23 7.064431287346295 7 24 9.067511166160333 7 25 8.485992013369632 7 26 6.84446968397063 7 27 7.784308787160409 7 28 5.129674168050279 7 29 6.033647660288693 7 30 6.4615357536877305 7 31 5.639081976745587 7 32 6.685546805709823 7 33 6.490158687397994 7 34 5.7377025362911525 7 35 5.830726467739829 7 36 8.219423798065876 7 37 7.452061018849651 7 38 9.163066385650957 7 39 9.839219303181412 7 40 8.713107740747361 7 41 14.502100696498115 7 42 13.651428472006327 7 43 13.072137924099618 7 44 13.462377740524815 7 45 12.977308574996872 7 46 15.268851186678475 7 47 14.640737688971686 7 48 16.336322598160958 7 49 14.642416102412017 7 50 14.815308833606213 7 51 14.849547384297246 7 52 14.420676734501665 7 53 15.068363044504064 7 54 15.540929875599446 7 55 14.663989117258016 7 56 15.286643755075502 8 1 3.7724361541972726 8 2 5.283469388709776 8 3 4.416656474878742 8 4 4.50593505634447 8 5 6.44329152016439 8 6 4.115951244054237 8 7 3.8525379475614585 8 8 4.46185055921022 8 9 4.614594990452744 8 10 4.11110511374547 8 11 4.442430828433743 8 12 5.078227460976976 8 13 5.782137729208344 8 14 4.946100123578979 8 15 5.632072327068741 8 16 5.663264970821945 8 17 7.80097233354682 8 18 7.706733205988382 8 19 6.246229711104192 8 20 5.312329599417119 8 21 4.596506730741588 8 22 6.105696605089037 8 23 4.309490575883842 8 24 6.541819466555591 8 25 7.136189641828132 8 26 5.0875526879232 8 27 2.6222534511920337 8 28 4.803587211528537 8 29 4.5949124824230765 8 30 2.8488875323438556 8 31 2.874259842358235 8 32 3.4740843551169327 8 33 1.3933207427339624 8 34 4.227821113366254 8 35 2.6943628313761963 8 36 4.984245932453227 8 37 5.584472086043331 8 38 4.8270980062043165 8 39 5.924199540335561 8 40 0.7387994403520999 8 41 9.444788443533689 8 42 8.714669578725694 8 43 8.44565571066088 8 44 7.571073392698729 8 45 6.172960748909919 8 46 8.48821877561128 8 47 9.478682353003311 8 48 9.035927341243962 8 49 8.017910173558263 8 50 9.263925434396725 8 51 8.211207463348625 8 52 8.20130914556886 8 53 8.920141700630545 8 54 9.343301417101422 8 55 9.8458072990762 8 56 8.962533083138744 9 1 4.195361055383046 9 2 5.297303380804642 9 3 6.381083352349094 9 4 3.7668046215618602 9 5 6.7496156308363275 9 6 5.740457172907995 9 7 4.150956919893474 9 8 3.944063997945399 9 9 4.606689852112822 9 10 6.281065588053767 9 11 5.025434781002907 9 12 5.831191887854445 9 13 6.108224685730086 9 14 5.4816732461086275 9 15 6.394918176871116 9 16 7.337178014300203 9 17 7.1487984205033435 9 18 8.368788541956757 9 19 8.31342338699352 9 20 7.312329599417119 9 21 6.914688548923408 9 22 7.870518739476388 9 23 6.9774747656071625 9 24 8.241424209638598 9 25 3.8338181003261553 9 26 5.409687075274974 9 27 5.219091395856065 9 28 5.520978515876365 9 29 5.294517225506084 9 30 5.157187927600777 9 31 6.552125455006461 9 32 4.3377207187533 9 33 6.011897817832775 9 34 5.911615579769418 9 35 4.6133351633920014 9 36 6.306380319805001 9 37 4.321626236240956 9 38 6.945675081303129 9 39 6.187045390137936 9 40 3.6696294798777913 9 41 10.154274609541591 9 42 10.564471950267194 9 43 10.246050967577876 9 44 10.549334262263947 9 45 10.368612922822965 9 46 12.312329447548038 9 47 13.901607254189084 9 48 13.205887815552263 9 49 13.903285667629408 9 50 14.46748274664969 9 51 14.327808253862457 9 52 14.203285430153844 9 53 13.285754348851892 9 54 14.41049509299075 9 55 14.96833694334497 9 56 11.286643755075502 10 1 4.499708881469999 10 2 5.45879406403445 10 3 5.931807990030258 10 4 4.50593505634447 10 5 6.8220793989522726 10 6 5.9557780838810785 10 7 4.763793358816876 10 8 5.1718072691669335 10 9 5.1470625229202795 10 10 5.877338879979231 10 11 4.994378880381795 10 12 5.539266422015942 10 13 5.853566300636913 10 14 4.169043846522705 10 15 6.987050682047098 10 16 7.496598304155281 10 17 7.80097233354682 10 18 8.505434504689678 10 19 7.315493780368264 10 20 7.836139123226644 10 21 7.533736167971028 10 22 7.934700934093371 10 23 7.387412653805924 10 24 7.632728557464684 10 25 5.991167996806489 10 26 4.020453120823632 10 27 4.016192845131425 10 28 5.303587211528537 10 29 5.590583478094075 10 30 4.844558528014854 10 31 4.227073695172088 10 32 4.813911194943774 10 33 4.525355374768594 10 34 5.050331935877075 10 35 3.7354883725017345 10 36 7.060003508210798 10 37 6.634255635826879 10 38 5.506751685857992 10 39 9.255368371504392 10 40 10.243128444681105 10 41 10.143922642667885 10 42 11.21250507656119 10 43 11.47379423879941 10 44 11.737740059365393 10 45 10.863436939386109 10 46 12.728478515871018 10 47 14.205955080276038 10 48 13.754542059858682 10 49 15.572022727670817 10 50 13.231457901929193 10 51 12.607311359452524 10 52 12.437239881499597 10 53 11.900661181150028 10 54 12.126851200651206 10 55 11.56875102201992 10 56 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117 4 3.7916493420587543 117 5 4.9173174941903675 117 6 4.051016179119173 117 7 3.4304600254835407 117 8 3.909902507262167 117 9 4.07563395149171 117 10 4.020196022836377 117 11 -0.291335405332493 117 12 -10.103590720841204 117 13 -11.50357655650594 117 14 -3.021432343953485 117 15 -7.17961598461957 117 16 -7.836735029178055 117 17 -0.27045623788175277 117 18 -4.923136923881749 117 19 -1.7083157434412612 117 20 -1.8305275434400237 117 21 5.414688548923408 117 22 6.982319981712415 117 23 5.673126939520209 117 24 4.4898714146075385 117 25 4.467358472996963 117 26 4.996643597014106 117 27 1.5638118927504756 117 28 3.946444354385683 117 29 5.971535859046455 117 30 2.796939480395803 117 31 4.1080260761244745 117 32 4.052006433039015 117 33 3.4063077557209738 117 34 4.597950983496126 117 35 5.116440753454114 117 36 4.393336841544134 117 37 4.896160397731638 117 38 6.268656447762758 117 39 6.469654085790108 117 40 7.790747492300152 117 41 5.834398833144078 117 42 5.045838409894525 117 43 -5.359539094533925 117 44 1.9282162498415865 117 45 2.5301036060527764 117 46 -1.0572357698432668 117 47 0.06309793741889536 117 48 -2.126410321093701 117 49 -1.7613106056625156 117 50 -0.6971135266422337 117 51 3.4644542165953816 117 52 3.746763691023407 117 53 2.472089752578597 117 54 4.888755962555969 117 55 2.1639891172580157 117 56 3.4170785376841977 118 1 2.4087997905609058 118 2 3.1471057523461425 118 3 4.598474656696922 118 4 2.778662329071743 118 5 4.57965515652803 118 6 2.9341330622360573 118 7 2.8979924930160053 118 8 2.6436687410284065 118 9 2.3418677177254708 118 10 2.65655965920001 118 11 -1.7848418988389874 118 12 -1.3308634481139308 118 13 -4.990589543518929 118 14 -6.008445330966474 118 15 -13.913382218385804 118 16 -10.109462301905328 118 17 -4.562664030089543 118 18 -5.611448612193438 118 19 -3.5719521070776246 118 20 -1.7785794914919713 118 21 5.096506730741588 118 22 7.332969332361763 118 23 5.582217848611116 118 24 7.087274012010138 118 25 6.5452805509190455 118 26 4.314825415195926 118 27 4.985889814828393 118 28 4.93995084789217 118 29 4.140367027877623 118 30 5.667069350525676 118 31 4.101532569630962 118 32 5.064993446026026 118 33 3.8933207427339624 118 34 4.500548386093527 118 35 6.012544649558009 118 36 7.393336841544134 118 37 4.993562995134237 118 38 6.645279824386137 118 39 7.242381358517381 118 40 11.32970853126119 118 41 0.12660662535186873 118 42 -0.37623951218339613 118 43 -4.2361624711573 118 44 -4.019835698210361 118 45 -0.4179483419991712 118 46 -9.011781224388722 118 47 -1.1576812833603256 118 48 -5.327709022392401 118 49 -2.6184534628053733 118 50 -3.3269836565123647 118 51 0.9839347360758985 118 52 2.246763691023407 118 53 2.238323518812365 118 54 0.2523923261923322 118 55 0.30035275362165237 118 56 2.780714901320561 119 1 2.583042214803335 119 2 2.601651206891596 119 3 4.098474656696922 119 4 3.7559350563444696 119 5 3.8220793989522726 119 6 3.3962542743572683 119 7 3.4661743111978254 119 8 3.302759650119313 119 9 3.5161101419679 119 10 3.8535293561697124 119 11 -0.23181159580868282 119 12 -6.686924054174536 119 13 -3.717862270791656 119 14 -5.152384724905868 119 15 -5.9058064608100445 119 16 -13.086735029178055 119 17 -1.532360999786512 119 18 -7.315994066738892 119 19 -3.9583157434412612 119 20 -3.2710037339162135 119 21 2.8313552155900723 119 22 2.613272362664798 119 23 2.006460272853545 119 24 2.6327285574646844 119 25 2.2649775206160143 119 26 2.6633102636807706 119 27 4.09952617846476 119 28 4.2202538781952015 119 29 0.8524882399988378 119 30 2.773129956586285 119 31 3.7985022666006643 119 32 1.4210540520866353 119 33 -0.04607319665997878 119 34 2.7050938406389804 119 35 1.2473931344064972 119 36 3.0600035082108015 119 37 2.372350873922116 119 38 4.637704066810379 119 39 5.6363207524567756 119 40 6.64789034944301 119 41 2.0963035950488376 119 42 2.7601241241802406 119 43 -3.811920046914878 119 44 -5.512259940634603 119 45 -1.6603725844234134 119 46 -2.390569103176599 119 47 -7.710711586390628 119 48 -3.13831508299846 119 49 -8.118453462805373 119 50 -2.1375897171184235 119 51 0.47635897850014075 119 52 2.080097024356739 119 53 0.32923260972145485 119 54 0.6387559625559689 119 55 0.8306557839246835 119 56 -0.5829214623158023 120 1 4.033042214803331 120 2 5.068317873558264 120 3 5.931807990030258 120 4 4.705935056344472 120 5 7.022079398952268 120 6 5.81292094102394 120 7 4.44950764453116 120 8 4.41942631678598 120 9 4.966110141967896 120 10 5.753529356169711 120 11 1.5848550708579836 120 12 3.12974261249213 120 13 -4.317862270791657 120 14 -3.535718058239201 120 15 1.2441935391899541 120 16 -2.770068362511388 120 17 3.600972333546821 120 18 -0.06599406673889163 120 19 2.1916842565587373 120 20 1.6456629327504508 120 21 6.381355215590077 120 22 8.229939029331462 120 23 6.073126939520208 120 24 7.232728557464686 120 25 6.648310853949347 120 26 4.629976930347439 120 27 6.749526178464759 120 28 5.836920544861869 120 29 5.952488239998836 120 30 5.5397966232529505 120 31 4.5318355999339985 120 32 6.3377207187533 120 33 6.020593470006688 120 34 6.355093840638979 120 35 2.164059801073165 120 36 6.126670174877468 120 37 5.405684207255451 120 38 6.287704066810377 120 39 8.702987419123442 120 40 9.347890349443013 120 41 -2.0370297382844953 120 42 -0.8732092091530923 120 43 0.7880799530851235 120 44 -9.328926607301272 120 45 1.3396274155765866 120 46 -2.757235769843266 120 47 -1.6607115863906294 120 48 0.47835158366820707 120 49 -11.418453462805374 120 50 0.14574361621490795 120 51 6.226358978500141 120 52 5.913430357690075 120 53 6.662565943054787 120 54 7.2887559625559675 120 55 5.530655783924683 120 56 9.017078537684199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 624.0 5 107.0 6 17.0 7 46.0 8 81.0 9 73.0 10 64.0 11 40.0 12 56.0 13 47.0 14 63.0 15 63.0 16 103.0 17 124.0 18 155.0 19 168.0 20 201.0 21 269.0 22 341.0 23 376.0 24 439.0 25 489.0 26 514.0 27 489.0 28 503.0 29 558.0 30 628.0 31 684.0 32 867.0 33 932.0 34 1033.0 35 1110.0 36 1183.0 37 1152.0 38 1077.0 39 677.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.79510225348443 0.8076071382050279 0.25400547088706527 0.1432851374234727 8 99.19239286179497 0.5014979809821545 0.07164256871173635 0.23446658851113714 9 97.88980070339977 0.4949850201901785 1.3091051191871825 0.3061091572228735 10 44.17090009118145 19.577960140679952 27.32838348313143 8.922756285007164 11 28.604923798358733 20.09248404324606 17.265859059528463 34.03673309886675 12 24.508271460205812 30.28526768268855 17.865051452390258 27.34140940471538 13 19.310928748208937 13.683730623941646 20.926143024618995 46.079197603230426 14 19.38257131692067 36.531197082193565 22.235248143806174 21.85098345707959 15 39.499149993461494 17.706290048384986 19.563227409441613 23.231332548711915 16 24.928292046936114 36.166883963494136 20.006518904823988 18.89830508474576 17 21.774824081313525 17.181391712275214 41.17148814177743 19.87229606463383 18 20.320437670965223 15.520385567278886 23.24475706656246 40.91441969519343 19 23.581155926239656 16.934905844790514 18.19248061510393 41.2914576138659 20 21.980959833072507 19.29447052686489 37.7999478351591 20.924621804903495 21 21.801719645648777 16.688379364252214 22.51823866597186 38.991662324127155 22 18.76384004168295 16.777387000130258 40.71251791064218 23.746255047544615 23 18.275367982284745 19.148104728409535 38.1789761625635 24.39755112674222 24 18.073466197733488 20.177152533541747 21.903087143415398 39.84629412530937 25 23.003777517259348 21.36902435847336 36.107854630715124 19.519343493552167 26 41.1442721230288 20.36361266779617 17.183630913593117 21.308484295581913 27 20.379301355578725 36.42466110531804 16.8274244004171 26.368613138686133 28 30.793278624462683 29.588380877947113 18.08649211931744 21.531848378272763 29 34.53110734315899 27.87270118690492 17.079692187296207 20.516499282639884 30 31.390952939642812 19.404249771868074 30.660930778255768 18.543866510233347 31 33.424514784421 28.3704572098476 19.76683600364726 18.438192002084147 32 34.24514784420998 28.298814641135863 18.366549433372413 19.08948808128175 33 32.650932324944584 27.637240839744425 19.142000260790194 20.569826574520796 34 23.96268267223382 20.002609603340293 37.94363256784969 18.0910751565762 35 21.790187891440503 20.694154488517746 40.17484342379959 17.34081419624217 36 29.982409277477363 20.125089582383218 30.301648315851192 19.590852824288227 37 30.13108980630014 17.33515945998826 31.865910128481055 20.66784060523055 38 33.76843754079102 20.499934734368882 26.7589087586477 18.972718966192403 39 31.73547251027196 19.454770755885995 19.956955585990997 28.852801147851043 40 25.159298430007226 16.61301977271234 27.300794849898182 30.926886947382247 41 22.28733210429286 14.682644192783775 24.512773242033184 38.51725046089018 42 23.341170323928946 27.57967607105538 21.185997910135843 27.893155694879834 43 17.695822454308093 26.135770234986943 29.471279373368148 26.697127937336813 44 20.85921663506179 26.541554959785525 27.48970117047015 25.10952723468253 45 27.329678935003916 19.733750978856694 30.005220569042024 22.931349517097363 46 17.67894461859979 28.50705329153605 24.059561128526646 29.754440961337515 47 16.754988550866862 25.97317631665031 21.13837095191364 36.133464180569185 48 28.013029315960914 22.293159609120522 21.218241042345277 28.475570032573287 49 18.18003786147921 27.93263267837326 21.81604543377505 32.071284026372474 50 20.471002674668927 30.347706960662794 22.088851197077435 27.092439167590843 51 19.886252206314964 29.267176570569394 23.958946198601033 26.887625024514612 52 21.16174457265793 34.630679966099486 23.645609231371015 20.56196622987157 53 19.796357940082242 33.35291430063312 24.025846876835715 22.824880882448927 54 20.790916209866875 21.82850430696946 28.778386844166015 28.602192638997654 55 20.023510971786834 22.08725182863114 31.45898641588297 26.430250783699062 56 20.84312190028713 19.16601409553641 33.0005220569042 26.99034194727225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 7.0 14 10.0 15 14.5 16 19.0 17 15.5 18 12.0 19 13.5 20 15.0 21 15.5 22 20.0 23 24.0 24 22.0 25 20.0 26 23.0 27 26.0 28 28.0 29 42.0 30 54.0 31 53.5 32 53.0 33 59.0 34 65.0 35 85.5 36 106.0 37 124.5 38 151.5 39 160.0 40 190.5 41 221.0 42 255.5 43 290.0 44 341.5 45 393.0 46 577.5 47 1163.0 48 1564.0 49 1641.5 50 1719.0 51 1399.5 52 1080.0 53 1061.5 54 984.0 55 925.0 56 912.0 57 899.0 58 866.5 59 834.0 60 861.0 61 888.0 62 861.5 63 762.5 64 690.0 65 635.0 66 580.0 67 584.5 68 589.0 69 473.5 70 358.0 71 339.0 72 271.0 73 222.0 74 202.5 75 183.0 76 128.5 77 74.0 78 53.0 79 36.0 80 40.0 81 42.5 82 45.0 83 28.0 84 11.0 85 5.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.3907776475185619 16 0.09118145108766446 17 0.039077764751856196 18 0.0 19 0.04559072554383223 20 0.11723329425556857 21 0.013025921583952065 22 0.0 23 0.0 24 0.0 25 0.0 26 0.05210368633580826 27 0.06512960791976032 28 0.0 29 0.13025921583952063 30 0.07815552950371239 31 0.0 32 0.0 33 0.10420737267161652 34 0.16933698059137683 35 0.16933698059137683 36 0.03256480395988016 37 0.13677217663149666 38 0.20841474534323304 39 0.13677217663149666 40 0.8531978637488603 41 1.0811514914680214 42 0.2735443532629933 43 0.2214406669271851 44 0.3972906083105379 45 0.19538882375928096 46 0.2735443532629933 47 0.4493942946463462 48 0.02605184316790413 49 0.2279536277191611 50 0.1628240197994008 51 0.3712387651426338 52 0.09769441187964048 53 0.21492770613520906 54 0.19538882375928096 55 0.2735443532629933 56 0.19538882375928096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 15354.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.92861794971995 #Duplication Level Percentage of deduplicated Percentage of total 1 89.43705400405251 66.11957796014069 2 6.113998766628491 9.039989579262734 3 1.85886706017091 4.122704181320828 4 0.8193110739141927 2.422821414615084 5 0.5726367720905647 2.1167122573922104 6 0.36120165624174083 1.6021883548261038 7 0.1585763368866179 0.8206330597889802 8 0.08809796493700996 0.5210368633580825 9 0.03523918597480398 0.23446658851113714 >10 0.5021584001409568 6.408753419304415 >50 0.02642938948110299 1.4589032174026313 >100 0.02642938948110299 5.132213104077113 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 334 2.175328904519995 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 313 2.038556727888498 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 141 0.9183274716686205 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 85 0.5536016673179628 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 74 0.48195909860622643 No Hit ACGAGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 65 0.4233424514784421 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 38 0.24749251009508924 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 37 0.24097954930311322 No Hit ACGAGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 37 0.24097954930311322 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT 32 0.20841474534323304 Illumina Single End Adapter 2 (95% over 24bp) ACGAGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 30 0.19538882375928096 No Hit ACGAGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 29 0.18887586296730494 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 28 0.1823629021753289 No Hit ACGAGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 27 0.1758499413833529 No Hit ACGAGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 25 0.1628240197994008 No Hit ACGAGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 24 0.15631105900742479 No Hit ACGAGCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 24 0.15631105900742479 No Hit ACGAGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 22 0.1432851374234727 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT 22 0.1432851374234727 Illumina Single End Adapter 1 (95% over 23bp) ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 21 0.13677217663149666 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT 21 0.13677217663149666 No Hit ACGAGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 20 0.13025921583952063 No Hit ACGAGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 20 0.13025921583952063 No Hit ACGAGCGGGATCCAAAGGGCTACTCTGGAGCCATTGCCTGATCCATCAGTGGTTAA 19 0.12374625504754462 No Hit ACGAGCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 19 0.12374625504754462 No Hit ACGAGCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 17 0.11072033346359254 No Hit ACGAGCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 17 0.11072033346359254 No Hit ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 16 0.10420737267161652 No Hit ACGAGCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 16 0.10420737267161652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006512960791976032 0.0 0.0 0.0 0.0 11 0.006512960791976032 0.0 0.0 0.0 0.0 12 0.019538882375928098 0.0 0.0 0.0 0.0 13 0.019538882375928098 0.0 0.0 0.0 0.0 14 0.039077764751856196 0.0 0.0 0.0 0.0 15 0.04559072554383223 0.0 0.0 0.0 0.0 16 0.04559072554383223 0.0 0.0 0.0 0.0 17 0.04559072554383223 0.0 0.0 0.0 0.0 18 0.04559072554383223 0.0 0.0 0.0 0.0 19 0.04559072554383223 0.0 0.0 0.0 0.0 20 0.04559072554383223 0.0 0.0 0.0 0.0 21 0.04559072554383223 0.0 0.0 0.0 0.0 22 0.05210368633580826 0.0 0.0 0.0 0.0 23 0.05861664712778429 0.0 0.0 0.0 0.0 24 0.05861664712778429 0.0 0.0 0.0 0.0 25 0.06512960791976033 0.0 0.0 0.0 0.0 26 0.06512960791976033 0.0 0.0 0.0 0.0 27 0.07815552950371239 0.0 0.0 0.0 0.0 28 0.08466849029568842 0.0 0.0 0.0 0.0 29 0.09118145108766446 0.0 0.0 0.0 0.0 30 0.09118145108766446 0.0 0.0 0.0 0.0 31 0.09769441187964048 0.0 0.0 0.0 0.0 32 0.11072033346359254 0.0 0.0 0.0 0.0 33 0.11723329425556858 0.0 0.0 0.0 0.0 34 0.12374625504754462 0.0 0.0 0.0 0.0 35 0.12374625504754462 0.0 0.0 0.0 0.0 36 0.12374625504754462 0.0 0.0 0.0 0.0 37 0.12374625504754462 0.0 0.0 0.0 0.0 38 0.13025921583952066 0.0 0.0 0.0 0.0 39 0.13025921583952066 0.0 0.0 0.0 0.0 40 0.13677217663149668 0.0 0.0 0.0 0.0 41 0.13677217663149668 0.0 0.0 0.0 0.0 42 0.13677217663149668 0.0 0.0 0.0 0.0 43 0.13677217663149668 0.0 0.0 0.0 0.0 44 0.14979809821544873 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATCC 20 3.7505373E-4 50.79 41 TCACTCC 15 0.0077737533 50.79 41 TTTTGTC 30 9.2101436E-7 50.453644 35 TTCACTC 15 0.0079816915 50.453644 40 TAGCGCT 15 0.0079816915 50.453644 37 TCTAGCG 15 0.0079816915 50.453644 35 CGCTAAT 15 0.0079816915 50.453644 40 ATCAGTG 15 0.0079816915 50.453644 45 GCGCTAA 15 0.0079816915 50.453644 39 CTAGCGC 15 0.0079816915 50.453644 36 TTGTCCA 20 3.8764044E-4 50.45364 37 TCCAATC 20 3.8764044E-4 50.45364 40 TTTGTCC 20 3.8764044E-4 50.45364 36 TGGTTAA 15 0.008087198 50.287132 50 GCCCAAA 15 0.008087198 50.287132 48 ACTCCGC 15 0.008087198 50.287132 43 CATCAGT 15 0.008087198 50.287132 44 TTGGGCC 15 0.008087198 50.287132 34 CCCAAAA 15 0.008087198 50.287132 49 CCATCAG 15 0.008087198 50.287132 43 >>END_MODULE