FastQCFastQC Report
Fri 17 Jun 2016
SRR1525981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT160.5029864822382898No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT80.2514932411191449No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT80.2514932411191449No Hit
ACGAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.22005658597925182No Hit
ACGAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.1886199308393587No Hit
ACGAGCGGGGGTATGATGCGCTCTGTGTGTGTGTGTGGCT60.1886199308393587No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT50.15718327569946558No Hit
ACGAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT40.12574662055957245No Hit
ACGAGCGGGGCTCTTTTTTCTCTTTGCTGTGTCCCTCTGT40.12574662055957245No Hit
ACGAGCGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT40.12574662055957245No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.12574662055957245No Hit
ACGAGCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.12574662055957245No Hit
ACGAGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCA40.12574662055957245No Hit
ACGAGCGGGGTACCGTGTCACTAGCAACTTGAAAAGGTGA40.12574662055957245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC251.420608E-434.6721320
GATCCTG251.420608E-434.6721319
TCCTGCC251.420608E-434.6721321
GTTGATC251.5696365E-434.11290416
TGGTTGA251.5696365E-434.11290414
GGTTGAT251.5696365E-434.11290415
TGATCCT251.5696365E-434.11290418
CTGGTTG251.5696365E-434.11290413
TTGATCC251.5696365E-434.11290417
GCGGGGG301.1664464E-533.571435
GCGGGGC301.1664464E-533.571435
GCGGGAT357.770559E-733.571435
GCGGGAG200.002543156533.571435
CGGGGTG200.002543156533.571436
GTACCTG251.7313543E-433.571439
CGGGGGG200.002543156533.571436
CGGGGAT251.7313543E-433.571436
GGGTACC251.7313543E-433.571437
ACCTGGT251.7313543E-433.5714311
CGGGTAC251.7313543E-433.571436
Produced by FastQC (version 0.11.5)