SRR1525978_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90169
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1627	1.804389535206113	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	1132	1.2554203772915302	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	1054	1.1689161463474143	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	405	0.44915658374829487	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	354	0.3925961250540651	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	231	0.2561856070268052	No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	213	0.23622309219354767	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	201	0.22291474897137598	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	199	0.22069669176768067	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	195	0.2162605773602901	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	172	0.19075291951779438	No Hit
CGTATTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	157	0.17411749049007974	No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	148	0.16413623307345096	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	138	0.15304594705497454	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	128	0.14195566103649815	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	112	0.12421120340693588	No Hit
CGTATTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	109	0.12088411760139293	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	108	0.1197750889995453	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	106	0.11755703179585002	No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.11644800319400238	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	102	0.11312091738845945	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	91	0.10092160276813537	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAATC	25	1.9388503E-5	50.563698	40
CGTCTCA	35	4.7746653E-8	50.563698	41
CGATTGA	35	4.7746653E-8	50.563698	40
ACCTAAT	25	1.9388503E-5	50.563698	40
TCCAATC	40	2.386514E-9	50.563694	40
AGACCTA	30	9.672312E-7	50.50676	38
GACCTAA	30	9.672312E-7	50.50676	39
TGTCCAA	45	1.200533E-10	50.506756	38
AGCGCTT	15	0.00809514	50.478336	37
GATGCGT	15	0.00809514	50.478336	37
TCAACGA	15	0.008240268	50.2521	45
CAACGAT	15	0.008258544	50.223965	46
TATGCTC	35	5.0356903E-8	50.22396	35
CTCAACG	15	0.00827685	50.195858	44
CTCCACG	20	4.083517E-4	50.195858	44
AACGATT	15	0.008295186	50.167786	47
GTAGTGG	20	4.1174897E-4	50.11173	14
ACGATTA	35	5.1706593E-8	50.0558	48
CGCGTCT	15	0.008368835	50.0558	49
CCACAAC	15	0.00840584	50.0	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content