##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90169
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.14258780733955	39.0	38.0	40.0	33.0	40.0
2	36.26226308376493	39.0	36.0	40.0	29.0	40.0
3	36.26603378101121	39.0	36.0	40.0	28.0	40.0
4	34.682729097583426	39.0	35.0	40.0	17.0	40.0
5	36.25207111091395	39.0	37.0	40.0	28.0	40.0
6	36.21156938637448	39.0	37.0	40.0	28.0	40.0
7	36.546130044693854	39.0	37.0	40.0	30.0	40.0
8	36.3926183056261	39.0	36.0	40.0	30.0	40.0
9	36.30343022546551	39.0	36.0	40.0	29.0	40.0
10	35.348035355831826	39.0	35.0	40.0	25.0	40.0
11	35.340238884760836	38.0	35.0	40.0	27.0	40.0
12	34.18893411261077	38.0	33.0	39.0	22.0	40.0
13	33.5949605740332	38.0	32.0	39.0	19.0	40.0
14	34.232729652097724	38.0	33.0	40.0	22.0	40.0
15	32.922079650434185	38.0	31.0	39.0	17.0	40.0
16	32.463141434417594	37.0	29.0	39.0	18.0	40.0
17	31.368286218101566	36.0	27.0	39.0	15.0	40.0
18	30.798400780756136	35.0	26.0	39.0	13.0	40.0
19	31.92872273175925	36.0	28.0	39.0	17.0	40.0
20	32.685279863367676	37.0	30.0	39.0	19.0	40.0
21	32.943206645299384	38.0	30.0	39.0	18.0	40.0
22	32.20284133127793	37.0	29.0	39.0	16.0	40.0
23	32.984895030442836	38.0	31.0	39.0	18.0	40.0
24	32.26239616719715	37.0	29.0	39.0	16.0	40.0
25	32.81163149197618	38.0	30.0	39.0	18.0	40.0
26	33.16475728909049	38.0	32.0	39.0	18.0	40.0
27	33.46103428007408	38.0	33.0	39.0	19.0	40.0
28	33.43022546551475	38.0	33.0	39.0	20.0	40.0
29	32.884927192272286	38.0	31.0	39.0	17.0	40.0
30	32.675553682529475	37.0	31.0	39.0	17.0	40.0
31	33.37283323536914	38.0	33.0	39.0	18.0	40.0
32	33.228870232563295	38.0	33.0	39.0	18.0	40.0
33	33.01053577171755	38.0	33.0	39.0	17.0	40.0
34	32.83942374873848	37.0	32.0	39.0	17.0	40.0
35	32.88665727689117	38.0	32.0	39.0	17.0	40.0
36	31.89220241990041	36.0	30.0	39.0	10.0	40.0
37	31.697002295689206	36.0	30.0	39.0	4.0	40.0
38	31.00662090075303	36.0	29.0	38.0	4.0	40.0
39	29.875001386285753	35.0	27.0	38.0	4.0	40.0
40	27.648016502345595	33.0	18.0	38.0	4.0	40.0
41	25.60438731714891	31.0	13.0	37.0	4.0	39.0
42	26.587086470960084	31.0	18.0	37.0	4.0	39.0
43	26.798822211624838	32.0	18.0	38.0	4.0	39.0
44	25.8576229080948	31.0	15.0	37.0	4.0	39.0
45	26.88784393749515	32.0	20.0	38.0	4.0	39.0
46	24.785380784970446	29.0	13.0	36.0	4.0	39.0
47	23.896084020006874	28.0	9.0	36.0	4.0	39.0
48	24.571571160820238	29.0	11.0	36.0	4.0	39.0
49	23.562277501136755	28.0	6.0	35.0	4.0	39.0
50	24.206534396522088	29.0	4.0	36.0	4.0	39.0
51	24.461732968093248	30.0	4.0	36.0	4.0	39.0
52	24.462908538411206	30.0	4.0	36.0	4.0	39.0
53	24.28506471181892	30.0	4.0	36.0	4.0	39.0
54	23.885326442568953	30.0	4.0	36.0	4.0	39.0
55	23.70229236212002	30.0	4.0	36.0	4.0	39.0
56	22.713371557852476	28.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.5586951529877098
1	2	3.5808262694939827
1	3	3.2425981817576144
1	4	5.288668068342865
1	5	3.70145510993715
1	6	3.9452981155633964
1	7	3.3283643657279285
1	8	3.4533255855029665
1	9	3.577381263675825
1	10	4.651008194741813
1	11	0.22804618072262173
1	12	-7.004558659386788
1	13	-18.444548547095923
1	14	-6.789394129643611
1	15	0.9635655612089842
1	16	-1.328536089077442
1	17	-5.929630824997595
1	18	-4.520190016714864
1	19	-0.027822930432058968
1	20	-0.715151330489384
1	21	6.21689052560129
1	22	6.958749734908679
1	23	6.297762005906883
1	24	7.153270320445429
1	25	6.250895827224024
1	26	6.6258183662474615
1	27	5.91892000271887
1	28	5.687225928705779
1	29	6.593066915282094
1	30	6.238236235383582
1	31	4.8083908045624
1	32	4.792383535356166
1	33	4.764512562472749
1	34	4.777698253469499
1	35	4.273365601895183
1	36	3.800886073406854
1	37	3.7126836748275913
1	38	3.9411969525935078
1	39	4.242026085296594
1	40	-9.55583920019285
1	41	0.18724030851913653
1	42	-0.8096111250252171
1	43	-8.944030960111105
1	44	-14.820835110017784
1	45	-2.081196080432189
1	46	-4.986450384120939
1	47	-11.475907652087804
1	48	-5.163463266594064
1	49	-13.411956391097211
1	50	-3.970632574000515
1	51	1.579145123591129
1	52	1.7296262697013738
1	53	1.9311482571059884
1	54	1.8557395679996276
1	55	1.8847331036275072
1	56	4.64499222525081
2	1	2.469123489998971
2	2	3.190569946289095
2	3	3.222624210162529
2	4	5.318374400035275
2	5	3.5297242210105395
2	6	3.9999011823884985
2	7	3.4331004037520287
2	8	3.330426247529786
2	9	3.6355490903040177
2	10	4.182666656802581
2	11	3.3225406072489605
2	12	-1.0265696365836412
2	13	1.182113592891632
2	14	3.9606200162286243
2	15	4.471911625827328
2	16	3.9297675379241994
2	17	6.325956794535024
2	18	4.704276683901895
2	19	6.199246610675846
2	20	4.93765238138749
2	21	6.495111540709079
2	22	7.403892042383362
2	23	6.7466954071404
2	24	7.1213572326844385
2	25	6.610766816869248
2	26	6.3916193055333785
2	27	5.800490760371787
2	28	5.85697639551956
2	29	6.201792089276324
2	30	6.60450115927879
2	31	5.025275317661482
2	32	4.851286947340547
2	33	5.016309088246526
2	34	4.256620903840119
2	35	5.336003524149465
2	36	5.761107880683486
2	37	6.080136851990492
2	38	6.437943401979574
2	39	6.80899264445463
2	40	5.641241091777953
2	41	9.692926949157396
2	42	8.192765381510178
2	43	7.606809304699624
2	44	-2.835999485053147
2	45	8.001528980395065
2	46	7.306142837177088
2	47	3.5793744166045514
2	48	6.019527736631197
2	49	-0.8312400441272558
2	50	6.63877160186097
2	51	9.595441168405252
2	52	8.1515693467115
2	53	8.696609695458278
2	54	8.498301668378737
2	55	8.843766657636447
2	56	9.52729856825992
3	1	2.8312872736206103
3	2	3.659673035980127
3	3	3.7173247401095324
3	4	5.042252012274048
3	5	3.7725499384388
3	6	4.1526359089158476
3	7	3.5757361401783854
3	8	3.581551135041039
3	9	3.559806664546592
3	10	4.899494973970867
3	11	3.931879673342351
3	12	5.051722534199278
3	13	5.76820498375254
3	14	4.9878172965005945
3	15	1.086800136976219
3	16	6.586351879490039
3	17	8.41819757045743
3	18	8.428254286141673
3	19	7.454921043232591
3	20	6.674728673758249
3	21	7.267384313431808
3	22	8.227359695618034
3	23	7.256294447236392
3	24	7.935525815826125
3	25	7.208207072843646
3	26	6.497008766587275
3	27	5.8034904604017825
3	28	5.909271166042508
3	29	6.548307437741478
3	30	6.1937422351712
3	31	5.02767507768548
3	32	5.963775698465433
3	33	5.518308888266528
3	34	5.588837682162282
3	35	5.7929578287190076
3	36	6.131670824389122
3	37	6.399154950180677
3	38	6.799007295590215
3	39	7.3485886848505935
3	40	8.909914224464686
3	41	11.831713070545256
3	42	10.172867371311199
3	43	10.525067478882207
3	44	10.755091405857758
3	45	10.558073325960507
3	46	11.745098941566646
3	47	11.606071746871525
3	48	11.06272341706315
3	49	12.226304201448183
3	50	11.098975581463012
3	51	10.680132699252166
3	52	10.61122338130804
3	53	10.05337401902592
3	54	10.078793619183656
3	55	10.395511483153896
3	56	9.780173280788667
4	1	3.1244181054649616
4	2	3.8487736911320027
4	3	3.8878627587714334
4	4	5.563693346401507
4	5	3.9761433472138634
4	6	4.306100272624398
4	7	3.580302350224052
4	8	3.537080219813724
4	9	3.7764038454082183
4	10	4.560007183619462
4	11	4.320900916145611
4	12	5.3087207474214395
4	13	5.810591324828579
4	14	5.360757538708256
4	15	6.226962932290885
4	16	7.4988990305720336
4	17	8.488973826165193
4	18	8.753150057434105
4	19	7.6705255697364585
4	20	6.976793394822376
4	21	6.936135554249756
4	22	8.17782624316618
4	23	6.9531378063787415
4	24	7.686468837465995
4	25	6.674556090115832
4	26	6.229289886301391
4	27	5.704225024626524
4	28	5.746376585935302
4	29	6.4703840706579
4	30	5.907262187523791
4	31	5.259856931963817
4	32	5.0456696829800265
4	33	5.667107776493648
4	34	5.50823414831277
4	35	6.036257411804183
4	36	6.574394667946795
4	37	6.827783101858262
4	38	7.1744291736922605
4	39	7.683212468849003
4	40	1.0890883360390333
4	41	11.396037072927626
4	42	10.452101042147014
4	43	10.469181038540732
4	44	11.354257576197245
4	45	10.04923870217651
4	46	13.185440414665713
4	47	13.063189078616613
4	48	12.973880852044292
4	49	12.70853568844441
4	50	12.93280524197522
4	51	12.45966416639531
4	52	12.236198564949095
4	53	12.01068988164256
4	54	11.794398145687524
4	55	12.531008802987131
4	56	11.267169508702107
5	1	3.003484128009241
5	2	3.6597237716473003
5	3	3.6996179922658
5	4	5.453870480056899
5	5	3.9207488222541187
5	6	4.114634894202503
5	7	3.6877097576314597
5	8	3.5005262745641232
5	9	3.6883201094983917
5	10	4.655659357532507
5	11	4.474041012763969
5	12	5.384244096857827
5	13	5.919448007598305
5	14	5.82404256286285
5	15	6.987510436316651
5	16	7.75880241221455
5	17	8.911196048387417
5	18	9.078431860976778
5	19	7.820928146225992
5	20	6.953927050216901
5	21	7.134203187099995
5	22	7.925330268931077
5	23	7.043153909438324
5	24	7.691943877723638
5	25	6.961834673046592
5	26	6.3605298218891555
5	27	5.508411820117665
5	28	5.394202672891822
5	29	6.274892927340666
5	30	6.459597131163086
5	31	4.471612165458183
5	32	5.157585947070203
5	33	4.9376391774598325
5	34	4.475544937362688
5	35	5.052521501981314
5	36	6.735425263759996
5	37	6.796060074483055
5	38	6.95446137981142
5	39	8.717995077544654
5	40	10.01453117017752
5	41	12.329531436856776
5	42	11.210716179023017
5	43	11.443738204402244
5	44	11.91577126379789
5	45	10.844729845493738
5	46	11.804764086163303
5	47	12.716168144639159
5	48	13.046183589564418
5	49	13.73188512483733
5	50	13.074673196886653
5	51	11.79154822436632
5	52	11.741512574610933
5	53	11.084119833333379
5	54	12.026282203658539
5	55	12.379479012326904
5	56	11.925784645578116
6	1	3.1270531515782665
6	2	3.8938988558223855
6	3	4.055846948494754
6	4	5.914813240999663
6	5	4.1885939400992385
6	6	4.58113321070082
6	7	4.01969628961799
6	8	3.9244319984698492
6	9	4.0886568098350935
6	10	4.876871478744633
6	11	5.0129299016528535
6	12	5.470776083389815
6	13	6.449919388069688
6	14	5.217476906297193
6	15	6.896264645070861
6	16	7.242640796052932
6	17	8.973822311013677
6	18	9.6003173828623
6	19	8.190288415586263
6	20	7.619415265705118
6	21	7.265516318413123
6	22	8.002266295867102
6	23	7.64483741112182
6	24	8.099182934962698
6	25	7.466716827928746
6	26	6.7477352090945395
6	27	6.273394985100829
6	28	6.601778430467583
6	29	6.706879459327197
6	30	6.377947299513252
6	31	5.056629000475013
6	32	5.373074162558417
6	33	5.515922005742656
6	34	5.342885004702762
6	35	5.493767293227105
6	36	6.608320886655623
6	37	6.937137515560497
6	38	6.921464746814792
6	39	7.958904168453746
6	40	5.792645648291998
6	41	12.328184635509977
6	42	10.82957139787824
6	43	11.23532069598474
6	44	11.866444664471292
6	45	10.896076646840541
6	46	12.899208530607744
6	47	13.133676562147574
6	48	12.803590996971831
6	49	13.786598929551129
6	50	12.90480787702133
6	51	11.691548224366326
6	52	11.375856008954369
6	53	12.251796601010145
6	54	12.632342809719148
6	55	12.024091806939701
6	56	11.447670167463635
7	1	2.6892541085160673
7	2	3.6317092910012363
7	3	3.5894536926278136
7	4	5.698704788049106
7	5	3.594210253610285
7	6	3.8117666123868474
7	7	3.122908866514777
7	8	2.9013060016596413
7	9	3.09606168566102
7	10	4.218065372570102
7	11	3.562428648520026
7	12	4.769819145590773
7	13	5.0323413520705955
7	14	5.066816163531186
7	15	5.616360338850765
7	16	6.665402245130167
7	17	8.040352272280487
7	18	8.930005238865952
7	19	6.957547472319003
7	20	6.459412987281784
7	21	6.514320146164319
7	22	6.748165133871204
7	23	6.27334048699332
7	24	6.858638391786577
7	25	6.368573742943557
7	26	4.907452684601491
7	27	4.162435768878453
7	28	4.737059815748971
7	29	5.290041910910702
7	30	5.415598245058938
7	31	3.2170869635645545
7	32	4.074600706190225
7	33	4.7137461114615
7	34	4.295038114750607
7	35	3.602618967868249
7	36	5.069872492944068
7	37	5.067349408930287
7	38	5.501551326901371
7	39	6.203208110126109
7	40	7.098296138153017
7	41	9.50407891666741
7	42	9.68033466969414
7	43	8.824634890562088
7	44	10.457068952464002
7	45	9.47268863134726
7	46	10.897328831359623
7	47	10.879632360734949
7	48	9.960859167397896
7	49	11.003675712417387
7	50	10.305639501537168
7	51	9.279267522611939
7	52	10.063484170266737
7	53	9.693810727234798
7	54	10.27690877007458
7	55	8.389949633323841
7	56	7.9753530109359545
8	1	2.89978042430554
8	2	3.7242996378360687
8	3	4.178769724643338
8	4	5.970776095349613
8	5	4.2645958951334535
8	6	4.28763369945937
8	7	3.7953561136649085
8	8	3.7115776485187197
8	9	3.9202794882319623
8	10	4.731465809145412
8	11	4.757553557566837
8	12	5.324148517407409
8	13	6.186234148577995
8	14	5.671533602289372
8	15	6.314153208120718
8	16	7.4941070717127545
8	17	8.193274901434009
8	18	9.005253844250376
8	19	7.832517154589194
8	20	6.452015461208536
8	21	7.485457667386736
8	22	8.021327879444812
8	23	7.187359405256721
8	24	7.735790950602976
8	25	7.300783299091993
8	26	6.788546548292974
8	27	6.636786969460552
8	28	6.045815576117633
8	29	7.719098423159068
8	30	7.191974669992234
8	31	5.687860672029267
8	32	5.643846401072594
8	33	6.116850646993878
8	34	6.140538963647042
8	35	6.075878730177248
8	36	6.978555490761192
8	37	7.077899978903606
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115	9	3.0275282831893264
115	10	3.703935219601476
115	11	2.2112057637218214
115	12	-1.055347218595557
115	13	-1.8235915837171461
115	14	-3.4295972838804474
115	15	-6.59537589829382
115	16	2.4584192704520973
115	17	3.580927849153703
115	18	4.184434415255197
115	19	3.074823420810926
115	20	3.0172731549423197
115	21	5.034586328862449
115	22	6.206811750412555
115	23	5.879424662950456
115	24	6.233195474147649
115	25	5.735525605358209
115	26	4.47291807220844
115	27	4.653750262007833
115	28	4.398800374041251
115	29	4.439899313036705
115	30	4.638396620307404
115	31	4.317078321269165
115	32	4.03242630466918
115	33	3.7460682962337764
115	34	5.0482141583077755
115	35	3.476787146936612
115	36	5.3280178563525915
115	37	4.864514736041169
115	38	5.889071852352927
115	39	7.232937606280284
115	40	8.053356202106002
115	41	9.377296443242471
115	42	7.4868336758301695
115	43	6.223303977071467
115	44	-2.6035135382455294
115	45	7.367079781636779
115	46	1.867599335205444
115	47	-7.6771907314783405
115	48	2.4443955946729794
115	49	-2.81345331705284
115	50	3.1349541675124506
115	51	4.249019488734138
115	52	4.738447440511315
115	53	4.59139952681997
115	54	4.750420134693023
115	55	5.039504555111069
115	56	7.066269958477221
116	1	2.6051375671626857
116	2	3.4155438774674067
116	3	3.3112582039060072
116	4	4.368817570003991
116	5	3.52842077992608
116	6	3.6393042063718113
116	7	3.1487547311764317
116	8	3.0004601369979866
116	9	3.0199338661121473
116	10	3.853873643246793
116	11	3.033763234986189
116	12	3.1452890704028036
116	13	3.6709691716194683
116	14	3.9920981184184043
116	15	-3.6970795107732926
116	16	3.6395563804685125
116	17	5.706235730927098
116	18	5.6095165170614365
116	19	4.628600103897956
116	20	4.669469378259233
116	21	6.374282552179363
116	22	6.733597464698271
116	23	6.066103644888059
116	24	6.972830121109887
116	25	5.949400810612726
116	26	5.695516594375928
116	27	4.890819227525071
116	28	4.69241695860611
116	29	5.347842662790406
116	30	5.412308771374725
116	31	4.924323805669822
116	32	4.408247322731576
116	33	4.772956637777288
116	34	4.773891498209249
116	35	4.9710400204998315
116	36	6.075041665876402
116	37	6.344605048028033
116	38	7.26940846975851
116	39	8.040614125163703
116	40	9.89058937123572
116	41	9.302481172306514
116	42	7.284657977964816
116	43	2.864108574772615
116	44	1.9819749675015963
116	45	6.514042014805906
116	46	1.059922816322029
116	47	-6.582773654302644
116	48	1.202400520781353
116	49	-3.2082603778081733
116	50	1.1796996519131078
116	51	4.554643462461563
116	52	4.892901463499822
116	53	5.021818246031792
116	54	4.484615536991875
116	55	4.808182716030608
116	56	7.129488349281818
117	1	1.7202932448183574
117	2	2.7021739140974432
117	3	2.4358003284481313
117	4	4.172388998575421
117	5	2.725307226812525
117	6	2.9989653785329864
117	7	2.4392767091984098
117	8	2.5409821150199647
117	9	2.13760785878614
117	10	3.4479670498402015
117	11	0.8142119529349046
117	12	-5.948804336190605
117	13	-7.126420938270641
117	14	-1.0511711123508256
117	15	-4.177161928355712
117	16	-7.1967531433410095
117	17	-0.40846207127070144
117	18	-2.0255567430118226
117	19	0.4793327046305542
117	20	-0.2378108415209894
117	21	3.04873310162991
117	22	4.181170724771526
117	23	3.3254900917745047
117	24	4.170861256641018
117	25	3.708558319770237
117	26	3.8747748361341685
117	27	3.2594089711148158
117	28	3.3249719036610585
117	29	3.5238957763435153
117	30	3.7060358776018276
117	31	2.9744611683071867
117	32	3.4837967732810284
117	33	3.5701178099384627
117	34	3.4407871026048547
117	35	3.0864246358844447
117	36	4.686992215326949
117	37	4.3194218978448795
117	38	5.431954257304298
117	39	5.887225846775426
117	40	7.956981312627661
117	41	7.658021465346803
117	42	6.058009626316466
117	43	-4.4499939893299505
117	44	0.39383394186057075
117	45	6.059829560593453
117	46	-0.41489403349482146
117	47	-0.756766328295317
117	48	0.43820638158721437
117	49	-0.5344966415444397
117	50	1.8820806042940603
117	51	6.12744566026376
117	52	5.9586065917049496
117	53	5.231983081196628
117	54	6.295512972889309
117	55	5.337028869876761
117	56	10.46282168261515
118	1	1.7901913832096525
118	2	2.5843305702266974
118	3	2.6693795346300817
118	4	4.323073930082273
118	5	2.5876184315894832
118	6	3.1183893335733757
118	7	2.2264210716852375
118	8	2.273331956958849
118	9	2.415971048108233
118	10	3.5296319602722335
118	11	1.3702358377259145
118	12	-4.441428150732225
118	13	-2.677000495699513
118	14	-7.6149224295268
118	15	-10.413199863023785
118	16	-7.557483736948313
118	17	-0.5886517446110595
118	18	-4.57859502892682
118	19	-0.24370909375371141
118	20	-0.09924392898147616
118	21	4.945466505212625
118	22	5.398592572330365
118	23	5.359034173263787
118	24	5.490320336374076
118	25	4.673960497501181
118	26	4.770981369327
118	27	5.022668542593564
118	28	4.6763944537137405
118	29	4.733238944590795
118	30	4.906071002294489
118	31	4.630414803712874
118	32	4.785693506684609
118	33	4.4566650526500915
118	34	4.787467819148585
118	35	4.724464678034078
118	36	5.446739317539805
118	37	5.693675498125881
118	38	5.346952501069666
118	39	7.21845169854922
118	40	8.951010114875643
118	41	7.544041837668548
118	42	6.446839974050924
118	43	0.14835515011507994
118	44	1.1044064743509097
118	45	7.640265106782422
118	46	-1.1727092776114354
118	47	0.0033320208441267596
118	48	-0.8002902815669835
118	49	-1.2462985382778413
118	50	0.9825372252986249
118	51	5.378762836238469
118	52	5.5564288607600965
118	53	5.258853471080716
118	54	5.8938621123343395
118	55	6.27222381192102
118	56	8.081543143802367
119	1	1.9238768098477053
119	2	2.781722879188571
119	3	2.7604836772278674
119	4	4.738654058816387
119	5	3.0994190587556787
119	6	3.2794720204854073
119	7	2.6867280530352957
119	8	2.451084061266485
119	9	2.880734210346226
119	10	3.629153333436122
119	11	1.5420463675163063
119	12	-5.527580981232308
119	13	-0.9554292793271735
119	14	-3.097510315316537
119	15	-7.439441817141624
119	16	-6.277074428481569
119	17	1.569295111305756
119	18	-3.310233913231162
119	19	0.6815260935709304
119	20	0.4716423558840148
119	21	5.44076275871981
119	22	6.580305726316169
119	23	5.705626020103203
119	24	5.25133700579147
119	25	5.7269395369466345
119	26	5.688954632241671
119	27	5.347144528729892
119	28	5.160467733132791
119	29	6.002425278969405
119	30	6.0843182378119
119	31	4.855369416685313
119	32	4.7845382005284804
119	33	4.983154572372815
119	34	4.979248641066395
119	35	4.175859297608266
119	36	5.344082113380704
119	37	5.556524153019428
119	38	5.892257006763678
119	39	6.776629615697264
119	40	9.010425860048116
119	41	6.661883065593948
119	42	5.740122693971703
119	43	-4.229264919203288
119	44	-0.32751298430563125
119	45	5.031253718764599
119	46	0.6974013016920821
119	47	-0.6060094546468768
119	48	-2.9664893243133115
119	49	-1.173514250441567
119	50	1.6009561063020925
119	51	5.617251035611304
119	52	5.517148451451629
119	53	5.630036834672065
119	54	5.070458910485851
119	55	5.622941752175187
119	56	8.725873891450494
120	1	1.6775582020833184
120	2	2.51651389510409
120	3	2.692210584858387
120	4	3.962512455365548
120	5	2.5059340074393077
120	6	2.601054647288919
120	7	2.0185739551623243
120	8	1.8980571387616507
120	9	2.3080731959181477
120	10	2.9964000982732486
120	11	-0.48398367859405766
120	12	-5.941681829068099
120	13	-4.421292733142433
120	14	-4.771019165532213
120	15	-1.071748822942606
120	16	-6.739963019884218
120	17	0.24206024591828168
120	18	-5.575889126677538
120	19	-0.3815409895764752
120	20	-1.1917519621287767
120	21	2.9737093599395017
120	22	4.466071009671815
120	23	3.6184625514136286
120	24	3.1931784456248735
120	25	3.302575413787327
120	26	4.669171797197794
120	27	3.984955029994204
120	28	3.557165635854787
120	29	4.3292139149949875
120	30	4.249720587953206
120	31	3.595068955581638
120	32	3.50326495941588
120	33	3.4808490540030412
120	34	3.855791850942939
120	35	4.230299279759091
120	36	4.624314152648882
120	37	4.650381062137381
120	38	4.924831750181795
120	39	5.451328410877988
120	40	6.036753392399742
120	41	-7.067999427340755
120	42	-4.130024561717722
120	43	-8.805644511647138
120	44	-14.024969476942848
120	45	-4.241689907592683
120	46	-9.634742086676207
120	47	-7.197412102274425
120	48	-6.884680607966443
120	49	-14.317497591212055
120	50	-4.72285766731088
120	51	0.2285852614033601
120	52	-0.5770059439075865
120	53	-0.3553863395061292
120	54	-0.39347088276121056
120	55	0.42392345677134813
120	56	2.7776364974299668
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2063.0
5	121.0
6	162.0
7	394.0
8	398.0
9	465.0
10	460.0
11	291.0
12	285.0
13	254.0
14	295.0
15	376.0
16	425.0
17	514.0
18	631.0
19	865.0
20	1049.0
21	1482.0
22	1825.0
23	2020.0
24	2444.0
25	2720.0
26	2933.0
27	3028.0
28	3029.0
29	3148.0
30	3555.0
31	3932.0
32	4528.0
33	5286.0
34	5663.0
35	6002.0
36	6471.0
37	7216.0
38	7672.0
39	8160.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57967815989974	0.2927835508877774	0.06321463030531557	0.06432365890716321
8	99.43994055606694	0.3160731515265779	0.1308653750180217	0.11312091738845945
9	97.77634833142946	0.5534175474397507	1.1134894140871938	0.5567447070435969
10	49.770985593718464	20.89298983020772	21.082633721123667	8.25339085495015
11	29.901629163016114	29.1330723419357	14.930852066674799	26.034446428373386
12	28.026261797291752	26.506892612760485	17.547050538433385	27.919795051514377
13	19.96584191906309	17.802127116858344	20.492630504940724	41.73940045913784
14	20.13552329514578	31.35223857423283	26.868435936962815	21.643802193658573
15	36.000980588799	18.854048271712244	21.73452786877939	23.410443270709365
16	28.462511652683446	29.259333244550984	22.02801083144671	20.250144271318863
17	25.17748591205573	19.831832098327197	35.42286018547278	19.567821804144295
18	23.755392651576486	19.964732890461246	23.970544200334924	32.309330257627344
19	27.070805689307033	21.088601415670002	20.4706326136641	31.369960281358868
20	22.133632416465858	27.733667951095462	30.296603111501003	19.83609652093767
21	22.222715123823043	20.65454868081048	26.974902682740186	30.147833512626292
22	21.14363029422529	20.142177466756866	32.351473344497556	26.362718894520288
23	21.36321795739112	23.148754006365824	30.57480952433763	24.91321851190542
24	20.973180416601966	22.976330442112737	25.115907628829387	30.93458151245591
25	26.886049555245002	21.81517712561833	29.23959095849693	22.05918236063974
26	34.15299940078564	21.197762933043343	19.309128032135643	25.340109634035375
27	21.179095035681385	31.366325205598038	19.386701885619793	28.067877873100784
28	26.53217399019586	33.716700308320206	18.514739480513718	21.23638622097021
29	34.46234170184403	27.216174183514774	18.662519440124417	19.658964674516774
30	28.749445184198848	22.981580115401687	28.75277407900577	19.516200621393697
31	29.749664481637993	31.425592564247605	19.772845750285605	19.05189720382879
32	35.54037115506206	26.89487637408348	19.267673126199377	18.297079344655078
33	30.922374834834166	26.650825551570602	19.039317795716237	23.387481817878992
34	22.823333333333334	26.39666666666667	31.994444444444444	18.785555555555554
35	22.12152511689379	24.731505236619686	36.320927598040846	16.826042048445675
36	27.51986152412232	28.50960898317873	24.688207358750166	19.28232213394878
37	28.560797076497575	23.573515200657567	29.629341656577324	18.236346066267537
38	33.182156712353134	23.80809461877925	24.686252931826015	18.323495737041608
39	28.017815887684378	23.16398613826195	20.095077305846807	28.72312066820686
40	23.35146387322052	18.34765869818247	27.850523771152297	30.450353657444712
41	20.002019386106625	16.576916173038953	25.093116137138754	38.32794830371567
42	24.6011792190455	27.34453220602959	22.278340193569917	25.77594838135499
43	18.157350357007807	23.852792668549945	30.660408835109106	27.32944813933314
44	19.137583406668078	25.51380735426808	26.96750621024607	28.381103028817773
45	25.440946019538323	22.0589706258266	31.022372384053703	21.47771097058137
46	20.046736771824403	27.556890891893392	23.140265954487287	29.256106381794915
47	17.716425729221235	27.47467091697411	22.35089557395869	32.458007779845964
48	25.117357866583806	26.57115271504511	24.24619072456691	24.06529869380417
49	20.09495430185239	28.897685071938444	22.463252462807713	28.544108163401454
50	23.611049379972442	27.26010044890884	24.06551402284546	25.063336148273258
51	19.57799145299145	31.370192307692307	23.998397435897438	25.053418803418804
52	20.320172417623926	31.862821338902837	27.700135534472416	20.11687070900082
53	19.411051677546347	30.50276347541786	28.4009652702868	21.685219576748995
54	19.618677042801558	27.38743746525848	28.454697053918842	24.539188438021124
55	19.497463961558996	25.569496351663997	29.442516462003915	25.490523224773092
56	22.99302938331721	23.11865612736106	28.821887958732173	25.06642653058956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.5
4	9.0
5	11.0
6	18.0
7	25.0
8	171.5
9	318.0
10	414.5
11	511.0
12	486.0
13	439.5
14	418.0
15	390.0
16	362.0
17	361.5
18	361.0
19	359.5
20	358.0
21	337.5
22	377.5
23	438.0
24	482.5
25	527.0
26	536.5
27	546.0
28	591.5
29	702.5
30	768.0
31	856.5
32	945.0
33	1126.0
34	1307.0
35	1398.5
36	1490.0
37	1499.5
38	1582.0
39	1655.0
40	1900.0
41	2145.0
42	2882.5
43	3620.0
44	5789.0
45	7958.0
46	7831.5
47	6415.0
48	5125.0
49	4760.5
50	4396.0
51	4545.5
52	4695.0
53	4720.0
54	4796.5
55	4848.0
56	5087.0
57	5326.0
58	5573.5
59	5821.0
60	5590.5
61	5360.0
62	4742.0
63	3756.0
64	3388.0
65	2922.0
66	2456.0
67	2198.5
68	1941.0
69	1648.5
70	1356.0
71	1167.0
72	777.5
73	577.0
74	440.5
75	304.0
76	232.0
77	160.0
78	138.0
79	78.5
80	41.0
81	25.5
82	10.0
83	6.5
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0022180572036952836
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.473555212988943
16	0.06765074471270614
17	0.023289600638800475
18	0.0
19	0.03881600106466746
20	0.12864731781432642
21	0.0
22	0.0
23	0.0
24	0.012199314620324057
25	0.0077632002129334925
26	0.05656045869422972
27	0.07319588772194435
28	0.0033270858055429254
29	0.16524526167529863
30	0.054342401490534445
31	0.011090286018476416
32	0.019962514833257547
33	0.1197750889995453
34	0.18742583371225144
35	0.1441737182401934
36	0.049906287083143874
37	0.15526400425866985
38	0.23067794918430945
39	0.1519369184531269
40	0.9060763677095232
41	1.1456265457086139
42	0.30941897991549205
43	0.2828022934711486
44	0.44139338353536134
45	0.21293349155474722
46	0.3371446949616831
47	0.49906287083143874
48	0.0665417161108585
49	0.2561856070268052
50	0.191861948119642
51	0.34712595237831184
52	0.17189943328638446
53	0.27282103605451985
54	0.2428772638046335
55	0.293892579489625
56	0.24398629240648118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	90169.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.84807417183289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86558239281186	62.95955372689062
2	6.554169340826197	9.28700551187215
3	1.9144373307452687	4.069025940178997
4	0.7998998168526839	2.2668544621765796
5	0.511873268318645	1.8132617640208941
6	0.26924220841225366	1.1445175171067663
7	0.2113238263700828	1.0480320287460214
8	0.12679429582204968	0.7186505339972717
9	0.12366357246841883	0.7885193359136733
>10	0.5682262886840005	7.077820536991649
>50	0.021915063475415993	1.0679945435792788
>100	0.028176510182677705	3.530038039681043
>500	0.0	0.0
>1k	0.004696085030446284	4.228726058845058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1627	1.804389535206113	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	1132	1.2554203772915302	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	1054	1.1689161463474143	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	405	0.44915658374829487	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	354	0.3925961250540651	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	231	0.2561856070268052	No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	213	0.23622309219354767	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	201	0.22291474897137598	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	199	0.22069669176768067	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	195	0.2162605773602901	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	172	0.19075291951779438	No Hit
CGTATTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	157	0.17411749049007974	No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	148	0.16413623307345096	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	138	0.15304594705497454	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	128	0.14195566103649815	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	112	0.12421120340693588	No Hit
CGTATTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	109	0.12088411760139293	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	108	0.1197750889995453	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	106	0.11755703179585002	No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.11644800319400238	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	102	0.11312091738845945	No Hit
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	91	0.10092160276813537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.008872228814781133	0.0	0.0	0.0	0.0
11	0.017744457629562265	0.0	0.0	0.0	0.0
12	0.01996251483325755	0.0	0.0	0.0	0.0
13	0.021071543435105193	0.0	0.0	0.0	0.0
14	0.049906287083143874	0.0	0.0	0.0	0.0
15	0.049906287083143874	0.0	0.0	0.0	0.0
16	0.051015315684991513	0.0	0.0	0.0	0.0
17	0.0532333728886868	0.0	0.0	0.0	0.0
18	0.055451430092382084	0.0	0.0	0.0	0.0
19	0.06210560170346793	0.0	0.0	0.0	0.0
20	0.06875977331455378	0.0	0.0	0.0	0.0
21	0.07097783051824906	0.0	0.0	0.0	0.0
22	0.07430491632379199	0.0	0.0	0.0	0.0
23	0.07874103073118256	0.0	0.0	0.0	0.0
24	0.08872228814781133	0.0	0.0	0.0	0.0
25	0.09426743115704954	0.0	0.0	0.0	0.0
26	0.09870354556444011	0.0	0.0	0.0	0.0
27	0.10535771717552596	0.0	0.0	0.0	0.0
28	0.12310217480508823	0.0	0.0	0.0	0.0
29	0.12975634641617406	0.0	0.0	0.0	0.0
30	0.1308653750180217	0.0	0.0	0.0	0.0
31	0.14750080404573634	0.0	0.0	0.0	0.0
32	0.14971886124943162	0.0	0.0	0.0	0.0
33	0.15082788985127926	0.0	0.0	0.0011090286018476416	0.0
34	0.1519369184531269	0.0	0.0	0.0011090286018476416	0.0
35	0.15304594705497454	0.0	0.0	0.0011090286018476416	0.0
36	0.15637303286051749	0.0	0.0	0.0011090286018476416	0.0
37	0.15859109006421276	0.0	0.0	0.0011090286018476416	0.0
38	0.15859109006421276	0.0	0.0	0.0011090286018476416	0.0
39	0.16080914726790804	0.0	0.0	0.0011090286018476416	0.0
40	0.16191817586975568	0.0	0.0	0.0011090286018476416	0.0
41	0.16302720447160332	0.0	0.0	0.0011090286018476416	0.0
42	0.17079040468453682	0.0	0.0	0.0011090286018476416	0.0
43	0.17633554769377502	0.0	0.0	0.0011090286018476416	0.0
44	0.191861948119642	0.0	0.0	0.0011090286018476416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAATC	25	1.9388503E-5	50.563698	40
CGTCTCA	35	4.7746653E-8	50.563698	41
CGATTGA	35	4.7746653E-8	50.563698	40
ACCTAAT	25	1.9388503E-5	50.563698	40
TCCAATC	40	2.386514E-9	50.563694	40
AGACCTA	30	9.672312E-7	50.50676	38
GACCTAA	30	9.672312E-7	50.50676	39
TGTCCAA	45	1.200533E-10	50.506756	38
AGCGCTT	15	0.00809514	50.478336	37
GATGCGT	15	0.00809514	50.478336	37
TCAACGA	15	0.008240268	50.2521	45
CAACGAT	15	0.008258544	50.223965	46
TATGCTC	35	5.0356903E-8	50.22396	35
CTCAACG	15	0.00827685	50.195858	44
CTCCACG	20	4.083517E-4	50.195858	44
AACGATT	15	0.008295186	50.167786	47
GTAGTGG	20	4.1174897E-4	50.11173	14
ACGATTA	35	5.1706593E-8	50.0558	48
CGCGTCT	15	0.008368835	50.0558	49
CCACAAC	15	0.00840584	50.0	13
>>END_MODULE