FastQCFastQC Report
Fri 17 Jun 2016
SRR1525977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7416
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA170.2292340884573894No Hit
CGTATTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT150.20226537216828477No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT110.1483279395900755No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG110.1483279395900755No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.1348435814455232No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC90.12135922330097086No Hit
CGTATTGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT80.10787486515641855No Hit
CGTATTGGGATGCCACAACTAGATACATCAACATGATTTA80.10787486515641855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAG200.002557696334.1496631
CTCTCCA200.002557696334.1496620
CTCCACC200.002557696334.1496622
TCTCCAC200.002557696334.1496621
AGCCTCT200.002645373333.9189217
GCCTCTC200.002645373333.9189218
GGTCTTT200.002645373333.918929
GGCCGAA251.8667174E-433.918929
TAGCCTC251.8667174E-433.9189216
ATTGGGT700.033.918924
ATTGGGC800.033.918924
GTATTGC200.002645373333.918922
TATTGGT200.002645373333.918923
TATTGGA200.002645373333.918923
TTGGGTG301.3164654E-533.918925
TTGGGTC200.002645373333.918925
TTGGGTA200.002645373333.918925
TGGGGTC200.002645373333.918926
TTGGGGT406.51944E-833.918925
TTGGGGC552.1827873E-1133.918925
Produced by FastQC (version 0.11.5)