FastQCFastQC Report
Fri 17 Jun 2016
SRR1525976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4652
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT140.3009458297506449No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.2794496990541703No Hit
CGTATTGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC130.2794496990541703No Hit
CGTATTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.21496130696474636No Hit
CGTATTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT100.21496130696474636No Hit
CGTATTGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC80.17196904557179707No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC60.1289767841788478No Hit
CGTATTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1289767841788478No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.1289767841788478No Hit
CGTATTGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC60.1289767841788478No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCCGTGGTCGCGCC50.10748065348237318No Hit
CGTATTGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10748065348237318No Hit
CGTATTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.10748065348237318No Hit
CGTATTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10748065348237318No Hit
CGTATTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT50.10748065348237318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAG251.6873944E-434.15217231
ATTGGGG1900.033.784954
TTGGGGG950.033.784955
GGTTGGG200.002593818133.78494612
GGGTTGG200.002593818133.78494611
ATTGGGT358.558145E-733.7849464
ATTGGGC502.6739144E-1033.7849464
TTGGGGT251.8013122E-433.7849465
TGGGGGG900.033.7849466
TTGGGCC358.558145E-733.7849465
TGGGGCC200.002593818133.7849466
TTGGGAT251.8013122E-433.7849465
TTGGGAC405.84605E-833.7849465
TTGGGAA301.2449822E-533.7849465
GGGGGTT200.002593818133.7849469
GGGGGGT200.002593818133.7849468
GGGGTTG200.002593818133.78494610
GTTGGGG200.002593818133.78494613
TGGGACC200.002593818133.7849466
CGTATTG4500.033.4095571
Produced by FastQC (version 0.11.5)