FastQCFastQC Report
Fri 17 Jun 2016
SRR1525975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1471.9602613681824244No Hit
CGTATTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG1311.7468995866115482No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1141.5202026936924924No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.8801173489798639No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC560.7467662354980664No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.600080010668089No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.54673956527537No Hit
CGTATTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT370.49339911988265106No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG250.33337778370449395No Hit
CGTATTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG250.33337778370449395No Hit
CGTATTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT200.26670222696359513No Hit
CGTATTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT170.22669689291905584No Hit
CGTATTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA170.22669689291905584No Hit
CGTATTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG160.21336178157087612No Hit
CGTATTGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT140.1866915588745166No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC140.1866915588745166No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG130.17335644752633686No Hit
CGTATTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT120.1600213361781571No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC100.13335111348179757No Hit
CGTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.13335111348179757No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.13335111348179757No Hit
CGTATTGGGATGCCACAACTAGATACATCAACATGATTTA90.12001600213361781No Hit
CGTATTGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG90.12001600213361781No Hit
CGTATTGGGGGGGGTCTGGTGTTCACCCTGCTTCTCATAG90.12001600213361781No Hit
CGTATTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.10668089078543806No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGAC80.10668089078543806No Hit
CGTATTGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT80.10668089078543806No Hit
CGTATTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT80.10668089078543806No Hit
CGTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10668089078543806No Hit
CGTATTGGGAAGCAGTGGTATCAACAAAAATCGACTTTTT80.10668089078543806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC200.00261572434.00000420
GGGTCAA200.00261572434.0000047
CCAGTAG200.00261572434.00000426
CCTGCCA200.00261572434.00000422
GAAGCAG406.3941116E-834.0000049
GGTCAAA200.00261572434.0000048
TAGCATA200.00261572434.00000430
TGGGGGC200.00261572434.0000046
TGGGGGA454.474714E-934.0000046
TTGGGCA200.00261572434.0000045
GATCCTG200.00261572434.00000419
AAGATCC200.00261572434.00000413
GGAAGCA406.3941116E-834.0000048
CTGCCAG200.00261572434.00000423
TGGGAGG200.00261572434.0000046
TCCTGCC200.00261572434.00000421
AAAGATC200.00261572434.00000412
GTAGCAT200.00261572434.00000429
GTTGATC251.8419194E-434.016
TGGTTGA251.8419194E-434.014
Produced by FastQC (version 0.11.5)