FastQCFastQC Report
Fri 17 Jun 2016
SRR1525974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14733
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2681.8190456797665104No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2371.6086336794950111No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1140.7737731622887396No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC940.6380234846942239No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA670.4547614199416276No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT570.3868865811443698No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA510.34616167786601504No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.31901174234711194No Hit
GAACGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG400.27149935518903145No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA360.2443494196701283No Hit
GAACGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC310.21041200027149937No Hit
GAACGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG310.21041200027149937No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG300.20362451639177356No Hit
GAACGCGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG260.17647458087287043No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC240.16289961311341886No Hit
GAACGCGGGGGAAAATGAATGAAAATAGGGCCTGTCTCCCCAGCCCTTTTTCTGCC240.16289961311341886No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.14932464535396728No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT220.14932464535396728No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC220.14932464535396728No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC220.14932464535396728No Hit
GAACGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC210.1425371614742415No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA200.13574967759451573No Hit
GAACGCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC190.12896219371478992No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10859974207561257No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA160.10859974207561257No Hit
GAACGCGGGAGATACGTTTATGTTCTCTGTTTAGGCCCAAGAAGGAAGCTTTGGTG160.10859974207561257No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG160.10859974207561257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTT150.00799159150.42758635
TGTCTCA150.00799159150.42758641
ATGCTTG150.00799159150.42758636
GCGCATT150.00799159150.42758638
CGCATTG150.00799159150.42758639
GCATTGA150.00799159150.42758640
GCCCTAT203.8810974E-450.4275838
CCAATCC203.8810974E-450.4275841
CCTATCA203.8810974E-450.4275840
TGTCCAA203.8810974E-450.4275838
GGCGCAT203.8810974E-450.4275837
TGCCCTA203.8810974E-450.4275837
TGGGCGC203.8810974E-450.4275835
CTGCCCT203.8810974E-450.4275836
GGGCGCA203.8810974E-450.4275836
CCCTATC203.8810974E-450.4275839
TTCGATG150.00810164550.25429550
GATTAAG203.94805E-450.25429550
TTCAGAG150.00810164550.25429547
TTTCGAT150.00810164550.25429549
Produced by FastQC (version 0.11.5)