##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14733
Sequences flagged as poor quality	0
Sequence length	56
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.04418652005702	38.0	35.0	39.0	30.0	40.0
2	33.59838457883662	38.0	33.0	39.0	15.0	40.0
3	33.70983506414172	38.0	33.0	39.0	16.0	40.0
4	35.59023959818096	38.0	35.0	39.0	28.0	40.0
5	35.169144098282764	38.0	35.0	39.0	27.0	40.0
6	35.54374533360483	38.0	35.0	39.0	27.0	40.0
7	35.57605375687233	38.0	35.0	39.0	29.0	40.0
8	35.5445598316704	38.0	35.0	39.0	28.0	40.0
9	35.67304690151361	38.0	35.0	39.0	28.0	40.0
10	34.60815855562343	38.0	35.0	39.0	24.0	40.0
11	34.4654177696328	38.0	33.0	39.0	25.0	40.0
12	33.810018326206475	38.0	32.0	39.0	22.0	40.0
13	33.34229281205457	38.0	32.0	39.0	19.0	40.0
14	33.891060883730404	38.0	33.0	39.0	21.0	40.0
15	32.71153193511165	37.0	31.0	39.0	17.0	40.0
16	32.54727482522229	37.0	30.0	39.0	18.0	40.0
17	31.390212448245435	36.0	27.0	39.0	15.0	40.0
18	30.959546596076834	35.0	26.0	39.0	14.0	40.0
19	32.07832756397204	36.0	29.0	39.0	17.0	40.0
20	32.817213059118984	37.0	30.0	39.0	20.0	40.0
21	33.400665173420215	38.0	32.0	39.0	19.0	40.0
22	32.59512658657436	38.0	30.0	39.0	17.0	40.0
23	33.3539672843277	38.0	32.0	39.0	19.0	40.0
24	32.56770515170027	37.0	30.0	39.0	17.0	40.0
25	33.12448245435417	38.0	31.0	39.0	18.0	40.0
26	33.36951062241227	38.0	33.0	39.0	18.0	40.0
27	33.67630489377588	38.0	33.0	39.0	20.0	40.0
28	33.802144844905996	38.0	33.0	39.0	22.0	40.0
29	33.109549989818774	38.0	32.0	39.0	17.0	40.0
30	32.995248761284195	38.0	31.0	39.0	17.0	40.0
31	33.62281952080364	38.0	33.0	39.0	19.0	40.0
32	33.54001221747098	38.0	33.0	39.0	18.0	40.0
33	33.289961311341884	38.0	33.0	39.0	18.0	40.0
34	33.2128554944682	38.0	33.0	39.0	17.0	40.0
35	33.232674947397	38.0	33.0	39.0	18.0	40.0
36	32.785787008755854	37.0	32.0	39.0	17.0	40.0
37	32.45849453607548	36.0	31.0	39.0	16.0	40.0
38	31.976922554808933	36.0	31.0	39.0	11.0	40.0
39	30.722867033190795	35.0	29.0	38.0	4.0	40.0
40	28.822710921061564	35.0	23.0	38.0	4.0	40.0
41	26.62580601371072	32.0	17.0	38.0	4.0	39.0
42	27.50967216452861	32.0	21.0	38.0	4.0	39.0
43	27.9233014321591	33.0	23.0	38.0	4.0	39.0
44	27.008484354849656	32.0	18.0	38.0	4.0	39.0
45	27.788569877146543	33.0	22.0	38.0	4.0	39.0
46	26.157741125364826	31.0	17.0	37.0	4.0	39.0
47	25.281816330686215	30.0	13.0	36.0	4.0	39.0
48	25.719405416412137	30.0	17.0	37.0	4.0	39.0
49	24.9653159573746	30.0	10.0	36.0	4.0	39.0
50	25.591393470440508	31.0	15.0	37.0	4.0	39.0
51	25.829973528812868	32.0	11.0	37.0	4.0	39.0
52	25.590986221407725	31.0	10.0	37.0	4.0	39.0
53	25.334487205592886	31.0	4.0	37.0	4.0	39.0
54	25.01907282970203	31.0	4.0	37.0	4.0	39.0
55	24.87524604629064	31.0	4.0	37.0	4.0	39.0
56	23.439761080567433	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.717096125056351
1	2	7.475524940142989
1	3	7.6194988019007255
1	4	4.388229050176399
1	5	4.530001311546577
1	6	4.553057445828486
1	7	4.211896116565015
1	8	4.402572106689213
1	9	4.292990180486271
1	10	5.250167800499312
1	11	-0.13903818371240106
1	12	-7.427992862179547
1	13	-17.624507883729606
1	14	-6.113722438550706
1	15	2.072981318972147
1	16	-1.2458761779840302
1	17	-4.18936832259266
1	18	-2.6982307328876303
1	19	1.124021648078731
1	20	-0.02935906849048564
1	21	6.907402855009462
1	22	8.011681626281181
1	23	6.734980490928521
1	24	7.436757389785779
1	25	6.040326600210534
1	26	4.969809225906683
1	27	6.892019086349531
1	28	6.773853442266368
1	29	6.777118856829887
1	30	7.1570921296150445
1	31	5.948058882943613
1	32	5.778157388757041
1	33	5.56208249063922
1	34	5.613669588413359
1	35	5.208497497535067
1	36	4.954753257693618
1	37	4.948897769407953
1	38	6.074354838303066
1	39	7.315037261305342
1	40	-10.641231519358389
1	41	-1.5443439872763634
1	42	-2.508276458293256
1	43	-10.41040362321936
1	44	-15.826067718543259
1	45	-1.916006763372824
1	46	-5.100544923386902
1	47	-11.97665097675455
1	48	-5.719416800567057
1	49	-15.642953821143276
1	50	-4.14501386546857
1	51	1.9249582772114486
1	52	1.3913583767407118
1	53	2.5931021340679408
1	54	1.7127620752888966
1	55	1.8929026805748599
1	56	7.66433817567966
2	1	4.270455808850819
2	2	7.714655374925599
2	3	7.550328841426413
2	4	5.068070947409595
2	5	4.690080362929976
2	6	4.709183928041931
2	7	4.294900069134179
2	8	5.0013863359382285
2	9	4.678365674557419
2	10	6.0367290653214525
2	11	2.708787903244122
2	12	-0.6750284353020781
2	13	1.3241087170609056
2	14	5.036475189907794
2	15	6.507763927667796
2	16	4.422108011739287
2	17	7.2711059857077345
2	18	4.461848318495772
2	19	6.641808209343555
2	20	5.869850417675526
2	21	6.138628151452149
2	22	7.944487950391853
2	23	6.28834017472299
2	24	6.9486150972956615
2	25	6.232026204953613
2	26	5.278109621163605
2	27	4.876208809669688
2	28	5.9833396082742745
2	29	7.150636643391152
2	30	7.396222564397654
2	31	4.491537143813176
2	32	5.282109957927002
2	33	5.259710949137244
2	34	5.309321762326405
2	35	4.61363583745602
2	36	6.650405431606664
2	37	6.439016346483054
2	38	5.400441794824808
2	39	7.579859395692694
2	40	5.975369271155444
2	41	11.016920834858023
2	42	9.97788954961188
2	43	8.915683333302383
2	44	-4.300376018938515
2	45	9.09782722872204
2	46	7.937004483727719
2	47	3.7012146358936704
2	48	7.669911262674049
2	49	-1.6251672598784523
2	50	4.6494525376934845
2	51	8.407171715946625
2	52	6.873571815475888
2	53	6.765038892961218
2	54	7.366912272917357
2	55	7.944286079784348
2	56	10.806630665798238
3	1	4.073998481958704
3	2	6.901450866068913
3	3	7.398960081362006
3	4	3.987555649502994
3	5	3.693300974846238
3	6	3.847670240441282
3	7	4.602468507137402
3	8	3.471595675712784
3	9	4.675145062641157
3	10	4.970706521038032
3	11	4.101702557028339
3	12	4.63092969674301
3	13	5.008488749267027
3	14	4.081563756735488
3	15	-2.075166829176002
3	16	6.377019444911593
3	17	7.327466694242354
3	18	8.168772634115737
3	19	6.699779223836305
3	20	5.763570224438812
3	21	5.299658747265358
3	22	7.466227080826634
3	23	6.42521618116421
3	24	7.283558736587128
3	25	5.856824916708845
3	26	5.616273872371334
3	27	5.67653087086131
3	28	6.036479704892631
3	29	4.664324244035278
3	30	5.916351388874304
3	31	4.7073181422028725
3	32	4.70239981299947
3	33	5.237166665723393
3	34	5.428484403228175
3	35	4.415568204605773
3	36	5.769568072508434
3	37	6.110513931024112
3	38	6.796577060525287
3	39	5.442983389251474
3	40	8.207253329126459
3	41	11.179561736629363
3	42	9.599467649450851
3	43	8.3488556360399
3	44	9.598174705699169
3	45	9.43921209184603
3	46	10.28160995876798
3	47	11.540184040080465
3	48	10.012906431756175
3	49	9.766137087947634
3	50	8.646231925777222
3	51	5.60845996071313
3	52	6.26004524542758
3	53	4.955054996020802
3	54	6.239698102224924
3	55	5.4080541957263755
3	56	7.196324707666193
4	1	4.819368852329077
4	2	8.35052494014299
4	3	8.278589710991632
4	4	5.274592686540032
4	5	4.795152826698093
4	6	5.102299870070915
4	7	4.579320358989257
4	8	4.9252993794164865
4	9	4.8649598774559735
4	10	5.549410224741742
4	11	5.902628482954263
4	12	6.6401889560022695
4	13	6.94367393445221
4	14	7.2574896826614115
4	15	7.822981318972147
4	16	8.326093518985669
4	17	10.15154076831643
4	18	9.775254115597217
4	19	8.783112557169641
4	20	8.360792446661033
4	21	8.017251339857946
4	22	8.591227080826634
4	23	8.212253218201248
4	24	9.251151329179716
4	25	7.532750842634776
4	26	7.560718316815777
4	27	6.3617160560465
4	28	6.758701927114853
4	29	6.909694614405645
4	30	6.823758796281709
4	31	5.198058882943613
4	32	3.9940664796661345
4	33	5.940870369427103
4	34	5.363669588413359
4	35	3.526679315716887
4	36	5.163086591026953
4	37	5.721625042135226
4	38	5.699354838303066
4	39	7.1559463522144355
4	40	0.5822533291264627
4	41	10.823080255147882
4	42	10.506875056858263
4	43	9.756263043447305
4	44	11.014841372365833
4	45	9.193841721475657
4	46	11.888091440249461
4	47	12.280924780821206
4	48	12.27679532064506
4	49	12.932803754614298
4	50	11.502713407258703
4	51	12.724200701453874
4	52	11.773934134316466
4	53	11.88098092194673
4	54	12.466549954076779
4	55	12.866387529059708
4	56	14.062065448406933
5	1	3.6110355189957417
5	2	7.475524940142989
5	3	7.361923044324968
5	4	4.274592686540032
5	5	4.295152826698093
5	6	4.352299870070915
5	7	4.454320358989257
5	8	4.716966046083151
5	9	4.198293210789302
5	10	5.50774355807507
5	11	5.569295149620935
5	12	4.7651889560022695
5	13	5.69367393445221
5	14	5.8824896826614115
5	15	6.156314652305483
5	16	6.576093518985669
5	17	8.15154076831643
5	18	9.650254115597217
5	19	7.866445890502977
5	20	7.3607924466610335
5	21	6.6839180065246175
5	22	8.091227080826634
5	23	6.9205865515345835
5	24	7.667817995846388
5	25	7.282750842634776
5	26	6.5190516501491125
5	27	4.4867160560465
5	28	5.217035260448188
5	29	5.159694614405645
5	30	6.0320921296150445
5	31	4.239725549610277
5	32	5.95239981299947
5	33	5.024203702760431
5	34	4.655336255080023
5	35	3.9433459823835513
5	36	3.2880865910269534
5	37	3.804958375468562
5	38	4.24102150496973
5	39	5.614279685547771
5	40	7.290586662459795
5	41	9.156413588481211
5	42	7.7152083901915915
5	43	7.422929710113976
5	44	8.056508039032497
5	45	7.985508388142328
5	46	7.263091440249461
5	47	9.572591447487877
5	48	8.27679532064506
5	49	9.682803754614298
5	50	8.669380073925367
5	51	8.515867368120539
5	52	9.482267467649802
5	53	8.089314255280062
5	54	9.716549954076779
5	55	10.283054195726379
5	56	10.687065448406933
6	1	4.486035518995742
6	2	8.10052494014299
6	3	7.903589710991632
6	4	5.274592686540032
6	5	5.1284861600314215
6	6	4.852299870070915
6	7	4.454320358989257
6	8	4.841966046083151
6	9	4.6149598774559735
6	10	5.299410224741742
6	11	5.569295149620935
6	12	6.348522289335605
6	13	6.485340601118875
6	14	6.8824896826614115
6	15	6.989647985638811
6	16	8.992760185652333
6	17	9.27654076831643
6	18	9.900254115597217
6	19	10.033112557169641
6	20	7.9857924466610335
6	21	7.642251339857946
6	22	8.79956041415997
6	23	7.628919884867912
6	24	7.959484662513052
6	25	7.241084175968105
6	26	7.1440516501491125
6	27	6.695049389379829
6	28	5.175368593781524
6	29	5.034694614405645
6	30	7.1570921296150445
6	31	5.156392216276949
6	32	4.285733146332799
6	33	3.649203702760431
6	34	5.155336255080023
6	35	4.276679315716887
6	36	0.5797532576936177
6	37	2.7216250421352264
6	38	2.6576881716364014
6	39	3.3642796855477677
6	40	1.5405866624597948
6	41	5.989746921814547
6	42	5.881875056858259
6	43	5.172929710113973
6	44	5.973174705699165
6	45	5.485508388142325
6	46	6.888091440249461
6	47	7.655924780821209
6	48	5.5684619873117285
6	49	6.516137087947634
6	50	6.877713407258703
6	51	5.224200701453871
6	52	6.190600800983134
6	53	6.630980921946726
6	54	6.049883287410111
6	55	7.0330541957263755
6	56	8.145398781740266
7	1	4.204368852329075
7	2	7.735524940142987
7	3	7.268589710991634
7	4	4.064592686540031
7	5	3.851819493364758
7	6	4.983966536737583
7	7	3.9209870256559185
7	8	4.01529937941649
7	9	4.8366265441226375
7	10	5.101076891408404
7	11	5.160961816287596
7	12	4.330188956002267
7	13	5.793673934452212
7	14	7.22082301599475
7	15	6.552981318972144
7	16	7.637760185652336
7	17	8.921540768316433
7	18	9.983587448930553
7	19	8.513112557169638
7	20	7.0924591133276955
7	21	6.425584673191285
7	22	7.466227080826634
7	23	6.542253218201246
7	24	7.609484662513051
7	25	7.2094175093014385
7	26	5.5007183168157745
7	27	6.008382722713161
7	28	6.912035260448189
7	29	6.088027947738979
7	30	8.257092129615046
7	31	6.048058882943614
7	32	5.529066479666131
7	33	5.527537036093769
7	34	4.89366958841336
7	35	5.486679315716888
7	36	5.674753257693617
7	37	5.861625042135227
7	38	6.734354838303062
7	39	8.965946352214438
7	40	4.700586662459791
7	41	12.20474692181455
7	42	11.033541723524927
7	43	9.169596376780639
7	44	11.504841372365835
7	45	8.962175054808995
7	46	11.083091440249461
7	47	12.01425811415454
7	48	11.115128653978399
7	49	11.286137087947637
7	50	10.407713407258704
7	51	10.97586736812054
7	52	11.282267467649799
7	53	11.427647588613397
7	54	12.55821662074344
7	55	12.754720862393043
7	56	13.41706544840693
8	1	4.308005215965444
8	2	7.202797667415716
8	3	7.710407892809812
8	4	3.4791381410854854
8	5	3.939092220637484
8	6	3.098511991283033
8	7	2.8937142983831947
8	8	3.3116630157801197
8	9	4.611171998668091
8	10	5.022895073226586
8	11	4.542779998105779
8	12	5.072007137820449
8	13	5.966401207179484
8	14	5.840823015994747
8	15	5.34570859169942
8	16	7.572305640197786
8	17	8.310631677407336
8	18	9.256314721657827
8	19	7.533112557169641
8	20	6.1979136587822445
8	21	5.498311945918555
8	22	7.284408899008454
8	23	6.780435036383068
8	24	7.6640301170585055
8	25	6.858508418392347
8	26	6.424354680452144
8	27	4.255655449985891
8	28	4.046580714993642
8	29	5.8225734022844335
8	30	5.384364856887771
8	31	4.49351342839816
8	32	3.8690664796661345
8	33	5.152991581548314
8	34	5.613669588413359
8	35	3.0721338611714337
8	36	3.227480530420891
8	37	4.0398068603170465
8	38	4.347082111030339
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115	32	4.235733146332802
115	33	4.274203702760431
115	34	5.21366958841336
115	35	4.260012649050221
115	36	3.688086591026952
115	37	3.4216250421352292
115	38	3.107688171636397
115	39	5.739279685547771
115	40	5.607253329126465
115	41	8.898080255147878
115	42	8.073541723524926
115	43	7.356263043447306
115	44	0.7981747056991679
115	45	3.335508388142326
115	46	1.0964247735827932
115	47	-7.0524085525121265
115	48	0.30179532064506276
115	49	-1.3005295787190327
115	50	-2.5389532594079647
115	51	-1.9841326318794614
115	52	-1.5177325323501982
115	53	-1.2523524113866067
115	54	-0.8417833792565581
115	55	0.07472086239304332
115	56	2.6703987817402677
116	1	4.515797423757647
116	2	7.546953511571559
116	3	8.028589710991632
116	4	3.5960212579686015
116	5	4.711819493364757
116	6	4.715395108166149
116	7	4.692415597084491
116	8	4.246727950845056
116	9	4.370912258408353
116	10	5.126791177122691
116	11	3.2895332448590295
116	12	2.604474670287985
116	13	5.122245363023641
116	14	4.983680158851893
116	15	-3.927018681027853
116	16	5.760617328509479
116	17	8.044397911173576
116	18	8.983587448930553
116	19	5.890255414312495
116	20	6.795316256184844
116	21	7.154156101762712
116	22	8.60908422368378
116	23	7.533681789629817
116	24	7.995198948227337
116	25	7.235131795015725
116	26	7.060718316815777
116	27	6.5283827227131646
116	28	7.092035260448188
116	29	7.653742233453265
116	30	7.79994927247219
116	31	5.876630311515044
116	32	4.654780765380419
116	33	5.893251321808052
116	34	5.970812445556213
116	35	5.812393601431172
116	36	7.526181829122187
116	37	5.864482184992372
116	38	5.217211981160212
116	39	7.548803495071581
116	40	8.897729519602649
116	41	8.507604064671693
116	42	7.602113152096358
116	43	0.23245351963778305
116	44	3.9076985152229753
116	45	7.902175054808993
116	46	3.834520011678034
116	47	-0.7428847429883163
116	48	1.520842939692681
116	49	3.2661370879476337
116	50	4.3419991215444185
116	51	6.5872959395491115
116	52	7.125124610506944
116	53	6.619076160041967
116	54	7.18678804931487
116	55	7.431863719535901
116	56	8.722779734121218
117	1	3.1586545666147927
117	2	6.90409636871442
117	3	6.957161139563063
117	4	2.881735543682886
117	5	3.640390921936188
117	6	3.0011093938804336
117	7	3.6209870256559213
117	8	3.5324422365593406
117	9	3.0851979726940684
117	10	4.055362605694121
117	11	-0.9247524694266858
117	12	-4.252668186854873
117	13	-4.592040351262074
117	14	-3.0163198411481105
117	15	-6.85559010959928
117	16	-9.739382671490525
117	17	-1.0984592316835702
117	18	0.19787316321626847
117	19	0.17596970002678347
117	20	-0.06182660095801751
117	21	6.297013244619851
117	22	7.537655652255204
117	23	7.105110361058394
117	24	7.280913233941622
117	25	6.592274652158586
117	26	5.703575459672923
117	27	4.885525579856019
117	28	4.520606689019619
117	29	5.725170804881834
117	30	5.942806415329329
117	31	4.662344597229328
117	32	5.154780765380419
117	33	5.0361084646651975
117	34	4.256526731270505
117	35	1.5981078871454564
117	36	3.311896114836472
117	37	3.5787678992780805
117	38	3.8600691240173504
117	39	4.4059463522144355
117	40	5.3263009481740795
117	41	4.793318350385974
117	42	4.459256009239212
117	43	-7.553260766076502
117	44	-1.735158627634167
117	45	4.973603626237566
117	46	-6.165479988321966
117	47	-0.17145617155974335
117	48	-2.5505856317358884
117	49	-3.4481486263380816
117	50	-0.6580008784555815
117	51	3.230153082406254
117	52	3.9822674676498018
117	53	2.476219017184821
117	54	5.043930906457728
117	55	4.646149433821616
117	56	9.222779734121218
118	1	3.4443688523290774
118	2	6.892191606809654
118	3	7.028589710991632
118	4	3.9412593532066964
118	5	3.7118194933647572
118	6	2.810633203404244
118	7	4.120987025655921
118	8	4.091966046083151
118	9	4.073293210789302
118	10	4.00774355807507
118	11	-0.22237151704573677
118	12	-9.60981104399773
118	13	-8.722992732214458
118	14	-6.992510317338585
118	15	-8.010352014361185
118	16	-8.465573147680999
118	17	0.06820743498309767
118	18	-2.016412551069447
118	19	0.5331125571696411
118	20	-0.43087422000563436
118	21	4.8089180065246175
118	22	7.466227080826634
118	23	5.962253218201248
118	24	6.8761513291797165
118	25	6.4494175093014405
118	26	6.1440516501491125
118	27	5.945049389379829
118	28	6.425368593781524
118	29	7.034694614405645
118	30	7.4070921296150445
118	31	6.198058882943613
118	32	6.45239981299947
118	33	4.940870369427103
118	34	4.613669588413359
118	35	5.860012649050223
118	36	5.954753257693618
118	37	6.054958375468562
118	38	6.824354838303066
118	39	7.6559463522144355
118	40	9.207253329126459
118	41	7.448080255147882
118	42	7.173541723524927
118	43	1.2562630434473085
118	44	-3.985158627634167
118	45	5.485508388142325
118	46	1.3464247735827932
118	47	-4.3024085525121265
118	48	-1.9315380126882715
118	49	-0.2338629120523663
118	50	2.794380073925371
118	51	4.015867368120539
118	52	3.6489341343164696
118	53	3.880980921946726
118	54	4.3415499540767755
118	55	0.5747208623930433
118	56	2.4370654484069334
119	1	3.372940280900508
119	2	5.975524940142989
119	3	6.385732568134486
119	4	3.0960212579686015
119	5	3.497533779079042
119	6	4.001109393880434
119	7	3.549558454227352
119	8	4.3181565222736324
119	9	3.870912258408353
119	10	4.19821974855126
119	11	-0.21046675514097046
119	12	-9.966953901140588
119	13	-2.020611779833505
119	14	-4.230605555433826
119	15	-9.498447252456426
119	16	-7.45366838577624
119	17	2.115826482602145
119	18	-2.3021268367837315
119	19	-0.10974458568750123
119	20	0.1524591133276978
119	21	4.368441816048428
119	22	4.537655652255204
119	23	5.390824646772678
119	24	5.066627519655906
119	25	5.306560366444295
119	26	5.8464326025300615
119	27	4.956954151284595
119	28	4.4491781175910425
119	29	5.43945651916755
119	30	6.014234986757899
119	31	5.876630311515044
119	32	5.797637908237565
119	33	4.893251321808052
119	34	5.756526731270505
119	35	5.598107887145456
119	36	6.454753257693618
119	37	5.650196470706657
119	38	6.645783409731635
119	39	9.048803495071581
119	40	10.469158091031225
119	41	5.364746921814547
119	42	3.1735417235249272
119	43	-0.33897505179078635
119	44	-2.30658719906274
119	45	4.402175054808993
119	46	-1.236908559750539
119	47	-3.8143133144168857
119	48	-8.122014203164461
119	49	-2.2338629120523663
119	50	-1.0865723070270121
119	51	3.3730102252633962
119	52	4.2679817533640865
119	53	3.4047904457562517
119	54	2.1867880493148704
119	55	3.0747208623930433
119	56	8.508494019835506
120	1	3.4443688523290774
120	2	6.908858273476319
120	3	6.961923044324962
120	4	3.957926019873362
120	5	3.41181949336476
120	6	4.010633203404247
120	7	2.8543203589892556
120	8	3.708632712749818
120	9	4.023293210789305
120	10	4.541076891408409
120	11	1.360961816287599
120	12	-4.5764777106643955
120	13	0.3936739344522131
120	14	1.3408230159947472
120	15	-0.9270186810278531
120	16	-1.1489064810143326
120	17	2.601540768316429
120	18	1.7169207822638874
120	19	4.033112557169641
120	20	3.319125779994362
120	21	5.025584673191279
120	22	6.399560414159964
120	23	5.462253218201248
120	24	4.409484662513055
120	25	6.5160841759681105
120	26	6.127384983482447
120	27	4.8617160560465
120	28	3.992035260448187
120	29	4.968027947738982
120	30	6.457092129615042
120	31	4.31472554961028
120	32	4.569066479666137
120	33	4.274203702760431
120	34	4.947002921746694
120	35	4.126679315716888
120	36	2.821419924360285
120	37	2.8882917088018907
120	38	3.24102150496973
120	39	4.0726130188811
120	40	6.540586662459795
120	41	-11.235253078185453
120	42	-7.5931249431417385
120	43	-10.71040362321936
120	44	-16.935158627634166
120	45	-9.397824945191008
120	46	-10.836908559750539
120	47	-10.252408552512126
120	48	-10.23153801268827
120	49	-18.1671962453857
120	50	-8.872286592741297
120	51	-5.650799298546129
120	52	-5.384399199016865
120	53	-4.852352411386605
120	54	-5.508450045923222
120	55	-3.658612470940291
120	56	-0.662934551593068
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	367.0
5	16.0
6	10.0
7	32.0
8	61.0
9	61.0
10	39.0
11	33.0
12	33.0
13	28.0
14	43.0
15	46.0
16	63.0
17	83.0
18	94.0
19	128.0
20	175.0
21	237.0
22	281.0
23	350.0
24	368.0
25	417.0
26	452.0
27	521.0
28	505.0
29	550.0
30	562.0
31	688.0
32	788.0
33	825.0
34	952.0
35	985.0
36	1108.0
37	1242.0
38	1338.0
39	1251.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.50451367678002	0.41403651666327296	0.0475123871580805	0.03393741939862893
8	99.3687639991855	0.5022738070997081	0.08144980655670943	0.0475123871580805
9	97.49507840608241	0.9571651619034689	1.0522028375534587	0.4955535944606612
10	39.998642503224055	30.815176813955063	21.98466028643182	7.201520396389059
11	24.061630353627912	24.679291386682955	21.495961447091563	29.763116812597566
12	22.846670739156995	30.163578361501393	20.219914477703117	26.769836421638498
13	17.47098350641417	18.943867508314668	22.74485848096111	40.84029050431005
14	15.841987375279984	38.56648340460191	22.93490802959343	22.656621190524675
15	32.84243539919547	24.572168814345126	19.710915661007704	22.874480125451694
16	23.27492529204021	38.42026623200218	19.987775061124694	18.317033414832924
17	20.558082694005027	24.685993618032455	36.567316178966664	18.188607508995858
18	18.061494603950315	24.882915903074732	21.618136156926624	35.43745333604832
19	18.517009574251375	25.762205472940856	21.511509472397634	34.20927548041013
20	17.282608695652176	28.41711956521739	36.12771739130435	18.172554347826086
21	18.32620647525962	24.265254870019685	23.912305708273944	33.49623294644675
22	18.448381185094686	24.699653838322135	35.86506482047105	20.986900156112128
23	16.887259892757754	29.44410507025046	34.202131269938235	19.466503767053553
24	15.116752647298398	31.149877816997012	21.470268802606572	32.263100733098014
25	19.676915767325053	29.695241973800314	31.765424557116678	18.86241770175796
26	33.50539971473205	29.953134551382192	17.978672824831897	18.56279290905386
27	19.33840510800163	42.25648689036816	18.55726124167912	19.847846759951093
28	26.729111518360142	37.19541166089731	18.543405959410848	17.532070861331704
29	29.146548792927575	35.45732743964638	17.64025841550493	17.755865351921116
30	25.626570671738097	29.022617673028595	29.08374651905182	16.267065136181486
31	25.307175344511574	37.051116692688886	19.971488697305002	17.670219265494534
32	28.841976649470542	35.67064892750475	18.81618245995113	16.671191963073582
33	27.802324790972744	35.57881857113724	19.148936170212767	17.469920467677248
34	20.00272053322451	30.14350812759301	34.523566619057334	15.330204720125145
35	18.407452740378076	29.137766897864818	36.65850673194615	15.796273629810962
36	24.438106878522444	29.35424730087594	26.841855096082025	19.36579072451959
37	24.144964982661318	28.48303528931801	29.217379479159582	18.154620248861086
38	28.651150129304476	28.45379066285559	25.79964611406016	17.095413093779772
39	25.92919752666984	29.59842359176463	19.0799755384929	25.39240334307264
40	19.90560229837882	24.078254326561325	26.554483890827008	29.46165948423285
41	18.356295839331	21.632736993625336	22.866543286037423	37.14442388100624
42	18.598951596432705	35.94526516440874	21.308462114507453	24.1473211246511
43	15.539989118607181	33.548694232861806	27.060663764961916	23.850652883569097
44	16.74158833946329	33.905462471052985	26.631249148617353	22.721700040866367
45	23.205171827152093	29.935352160598843	27.35624362027901	19.50323239197006
46	17.865160895299002	35.60106129668685	21.538880195931696	24.994897612082454
47	15.52358876465776	33.81510771748023	19.498227433869648	31.163076083992365
48	23.781067499660463	32.221920412875185	20.07333967132962	23.92367241613473
49	15.411820716860506	35.9994558933551	19.825885873631233	28.762837516153166
50	16.89671584959543	38.45107771809342	20.92200992724553	23.730196505065614
51	16.425022122387855	38.853719964604174	22.503573616499896	22.217684296508068
52	18.874464759056618	41.46673010263033	22.52429823965201	17.134506898661048
53	17.67628641437517	40.83855159270352	22.869588891913967	18.615573101007353
54	18.518770402611533	33.27665941240478	25.7480957562568	22.456474428726878
55	17.661471448989317	31.477574355135097	28.149458926019193	22.711495269856393
56	18.30985915492958	31.326120977070147	28.087364768320068	22.276655099680205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	21.0
13	88.5
14	137.0
15	200.0
16	263.0
17	263.0
18	263.0
19	261.5
20	260.0
21	219.0
22	161.0
23	144.0
24	146.0
25	148.0
26	137.5
27	127.0
28	141.5
29	144.5
30	133.0
31	143.0
32	153.0
33	174.0
34	195.0
35	217.0
36	239.0
37	249.5
38	271.5
39	283.0
40	301.5
41	320.0
42	344.0
43	368.0
44	404.5
45	441.0
46	570.5
47	1018.5
48	1337.0
49	1363.0
50	1389.0
51	1126.0
52	863.0
53	829.0
54	798.0
55	801.0
56	751.5
57	702.0
58	683.5
59	665.0
60	652.5
61	640.0
62	651.0
63	586.0
64	510.0
65	450.5
66	391.0
67	349.0
68	307.0
69	275.0
70	243.0
71	257.0
72	233.5
73	196.0
74	139.0
75	82.0
76	58.0
77	34.0
78	27.0
79	14.5
80	9.0
81	7.5
82	6.0
83	3.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.013574967759451571
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.44797393606190183
16	0.061087354917532075
17	0.027149935518903143
18	0.0
19	0.040724903278354714
20	0.08823729043643522
21	0.0
22	0.0
23	0.0
24	0.006787483879725786
25	0.0
26	0.06787483879725786
27	0.07466232267698364
28	0.0
29	0.190049548632322
30	0.06787483879725786
31	0.013574967759451571
32	0.006787483879725786
33	0.14932464535396728
34	0.20362451639177356
35	0.1832620647525962
36	0.040724903278354714
37	0.17647458087287043
38	0.2647118713093056
39	0.10859974207561257
40	0.7737731622887396
41	0.9773976786805132
42	0.29864929070793456
43	0.1968370325120478
44	0.34616167786601504
45	0.25792438742957985
46	0.23077445191067672
47	0.4411864521821761
48	0.0475123871580805
49	0.20362451639177356
50	0.17647458087287043
51	0.285074322948483
52	0.13574967759451573
53	0.27828683906875723
54	0.1968370325120478
55	0.27149935518903145
56	0.2443494196701283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	14733.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.6510554537433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7458602674223	68.79114912102084
2	6.623572124324803	10.154075884069776
3	1.602762773399451	3.685603746691102
4	0.7261135216505801	2.226294712550058
5	0.3719118037722483	1.4253716147424151
6	0.16824581599220756	0.7737731622887396
7	0.15939077304524926	0.8552229688454489
8	0.11511555831045782	0.7058983234914817
9	0.08855042946958293	0.6108735491753208
>10	0.3364916319844151	4.744451231928324
>50	0.035420171787833174	1.8258331636462364
>100	0.02656512884087488	4.201452521550261
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	268	1.8190456797665104	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	237	1.6086336794950111	No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	114	0.7737731622887396	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	94	0.6380234846942239	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	67	0.4547614199416276	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	57	0.3868865811443698	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	51	0.34616167786601504	No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.31901174234711194	No Hit
GAACGCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	40	0.27149935518903145	No Hit
GAACGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA	36	0.2443494196701283	No Hit
GAACGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	31	0.21041200027149937	No Hit
GAACGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	31	0.21041200027149937	No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	30	0.20362451639177356	No Hit
GAACGCGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG	26	0.17647458087287043	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	24	0.16289961311341886	No Hit
GAACGCGGGGGAAAATGAATGAAAATAGGGCCTGTCTCCCCAGCCCTTTTTCTGCC	24	0.16289961311341886	No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22	0.14932464535396728	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	22	0.14932464535396728	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	22	0.14932464535396728	No Hit
GAACGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	22	0.14932464535396728	No Hit
GAACGCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	21	0.1425371614742415	No Hit
GAACGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	20	0.13574967759451573	No Hit
GAACGCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	19	0.12896219371478992	No Hit
GAACGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16	0.10859974207561257	No Hit
GAACGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	16	0.10859974207561257	No Hit
GAACGCGGGAGATACGTTTATGTTCTCTGTTTAGGCCCAAGAAGGAAGCTTTGGTG	16	0.10859974207561257	No Hit
GAACGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	16	0.10859974207561257	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.013574967759451571	0.0	0.0	0.0	0.0
21	0.013574967759451571	0.0	0.0	0.0	0.0
22	0.013574967759451571	0.0	0.0	0.0	0.0
23	0.013574967759451571	0.0	0.0	0.0	0.0
24	0.027149935518903143	0.0	0.0	0.0	0.0
25	0.027149935518903143	0.0	0.0	0.0	0.0
26	0.033937419398628925	0.0	0.0	0.0	0.0
27	0.047512387158080496	0.0	0.0	0.0	0.0
28	0.054299871037806285	0.0	0.0	0.0	0.0
29	0.054299871037806285	0.0	0.0	0.0	0.0
30	0.054299871037806285	0.0	0.0	0.0	0.0
31	0.06108735491753207	0.0	0.0	0.0	0.0
32	0.06108735491753207	0.0	0.0	0.0	0.0
33	0.06108735491753207	0.0	0.0	0.0	0.0
34	0.06108735491753207	0.0	0.0	0.0	0.0
35	0.06108735491753207	0.0	0.0	0.0	0.0
36	0.06787483879725785	0.0	0.0	0.0	0.0
37	0.06787483879725785	0.0	0.0	0.0	0.0
38	0.06787483879725785	0.0	0.0	0.0	0.0
39	0.06787483879725785	0.0	0.0	0.0	0.0
40	0.06787483879725785	0.0	0.0	0.0	0.0
41	0.06787483879725785	0.0	0.0	0.0	0.0
42	0.07466232267698364	0.0	0.0	0.0	0.0
43	0.07466232267698364	0.0	0.0	0.0	0.0
44	0.08144980655670943	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCTT	15	0.007991591	50.427586	35
TGTCTCA	15	0.007991591	50.427586	41
ATGCTTG	15	0.007991591	50.427586	36
GCGCATT	15	0.007991591	50.427586	38
CGCATTG	15	0.007991591	50.427586	39
GCATTGA	15	0.007991591	50.427586	40
GCCCTAT	20	3.8810974E-4	50.42758	38
CCAATCC	20	3.8810974E-4	50.42758	41
CCTATCA	20	3.8810974E-4	50.42758	40
TGTCCAA	20	3.8810974E-4	50.42758	38
GGCGCAT	20	3.8810974E-4	50.42758	37
TGCCCTA	20	3.8810974E-4	50.42758	37
TGGGCGC	20	3.8810974E-4	50.42758	35
CTGCCCT	20	3.8810974E-4	50.42758	36
GGGCGCA	20	3.8810974E-4	50.42758	36
CCCTATC	20	3.8810974E-4	50.42758	39
TTCGATG	15	0.008101645	50.254295	50
GATTAAG	20	3.94805E-4	50.254295	50
TTCAGAG	15	0.008101645	50.254295	47
TTTCGAT	15	0.008101645	50.254295	49
>>END_MODULE