FastQCFastQC Report
Fri 17 Jun 2016
SRR1525973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA912.740138512496236No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA702.1077988557663354No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA672.0174646190906356No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTA672.0174646190906356No Hit
GAACGCGGGGATGAGATGGACTCCAAGGAATTGACGGGTA230.6925624811803673No Hit
GAACGCGGGGATGAGATGCACTTAAATGAATTGACGGGTA180.5420054200542005No Hit
GAACGCGGGGATGAGATGCACTTAAAGGAATTGACGGGTA170.5118940078289671No Hit
GAACGCGGGCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT150.45167118337850043No Hit
GAACGCGGGGATGAGATGGACTTCAAGGAATTGACGGGTA150.45167118337850043No Hit
GAACGCGGGGATGAGATGCACTCAAATGAATTGACGGGTA140.4215597711532671No Hit
GAACGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTA130.3914483589280337No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTA130.3914483589280337No Hit
GAACGCGGGGATGAGATGGACTTCAATGAATTGACGGGTA130.3914483589280337No Hit
GAACGCGGGGATGAGATGCACTCAAAGGAATTGACGGGTA120.36133694670280037No Hit
GAACGCGGGGATGAGATGGACTCCAATGAATTGACGGGTA110.331225534477567No Hit
GAACGCGGGGATGAGATGGACTTAACGGAATTGACGGGTA90.27100271002710025No Hit
GAACGCGGGATGAGATGGACTCAAATGAATTGACGGGTAC80.2408912978018669No Hit
GAACGCGGGGATGAGATGGACTCACATGAATTGACGGGTA80.2408912978018669No Hit
GAACGCGGGGATGAGATGGACTCAAATGAATTGACCGGTA80.2408912978018669No Hit
GAACGCGGGGATGAGATGGACTTAAAGGACTTGACGGGTA70.21077988557663355No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGCCGGGTA70.21077988557663355No Hit
GAACGCAGGGATGAGATGGACTTAAATGAATTGACGGGTA70.21077988557663355No Hit
GAACGCGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC70.21077988557663355No Hit
GAACGCGGGATGAGATGGACTTAAAGGAATTGACGGGTAC70.21077988557663355No Hit
GAACGCGGGGATGAGATGGACTTACATGAATTGACGGGTA60.18066847335140018No Hit
GAACGCGGGGATGAGATGGACTCAACGGAATTGACGGGTA60.18066847335140018No Hit
GAACGCAGGGATGAGATGGACTCAAATGAATTGACGGGTA60.18066847335140018No Hit
GAACGCGGGATGAGATGGCCTTAAATGAATTGACGGGTAC60.18066847335140018No Hit
GAACGCGGGATGAGATGGACTTAAATGAATTGACGGGTAC60.18066847335140018No Hit
GAACGCGGGGATGAGATGGACTCAACTGAATTGACGGGTA60.18066847335140018No Hit
GAACGCGGGGATGAGATGGACTTACAGGAATTGACGGGTA60.18066847335140018No Hit
GAACGCAGGGATGAGATGCACTTAAATGAATTGACGGGTA50.15055706112616682No Hit
GAACGCGGGGATGAGATGGACTCAAAGGACTTGACGGGTA50.15055706112616682No Hit
GAACGCAGGGATGAGATGCACTTAAAGGAATTGACGGGTA50.15055706112616682No Hit
GAACGCGGGGTACGGGAATGTAGGAACGTCCCTGTCTTGT50.15055706112616682No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACCGGTA50.15055706112616682No Hit
GAACGCGGGCAGAGTCGTTTTTTTTTTTTTTTTTTTTTTT50.15055706112616682No Hit
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTC40.12044564890093346No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACCGGTA40.12044564890093346No Hit
GAACGCGGGGATGAGATGGACCTAAAGGAATTGACGGGTA40.12044564890093346No Hit
GAACGCGGGCTCGTTTTTCTTTTTTTCTTTTTTTTTTTTC40.12044564890093346No Hit
GAACGCGGGCTGTAGCTTTGGCACGTCCCGAGTTCTTTGT40.12044564890093346No Hit
GAACGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT40.12044564890093346No Hit
GAACGCGGGGATGAGATGCACTCAAAGGACTTGACGGGTA40.12044564890093346No Hit
GAACGCGGGGATGAGATGGACCCAAAGGAATTGACGGGTA40.12044564890093346No Hit
GAACGCGGGATGAGATGGACTCAAAGGAATTGACGGGTAC40.12044564890093346No Hit
GAACGCGGGGATGAGCTGGACTCAAAGGAATTGACGGGTA40.12044564890093346No Hit
GAACGCAGGGATGAGATGGACTCCAATGAATTGACGGGTA40.12044564890093346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG200.002333159834.25
GCGGGGA1700.034.25
GCGGGAT251.5638264E-434.25
AACGCGG3100.034.22
TAAATGA251.5638264E-434.223
GGACTTA301.0378535E-534.218
GGACTCC200.002333159834.218
AGGAATT356.814553E-734.226
GAACGCG3200.034.21
TCAAAGG251.5638264E-434.222
CGGGATG200.002333159834.26
ACTTAAA251.5638264E-434.220
AAAGGAA301.0378535E-534.224
TGGACTT356.814553E-734.217
CAAAGGA200.002333159834.223
CGCGGGT356.814553E-734.24
CGCGGGC200.002333159834.24
CTTAAAT200.002333159834.221
AAGGAAT301.0378535E-534.225
ACGCGGG3050.034.23
Produced by FastQC (version 0.11.5)