##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525973_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3321 Sequences flagged as poor quality 0 Sequence length 40 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.739837398373982 30.0 22.0 33.0 4.0 35.0 2 17.577838000602227 19.0 4.0 26.0 4.0 31.0 3 19.618789521228546 22.0 4.0 29.0 4.0 33.0 4 17.988858777476665 17.0 4.0 29.0 4.0 33.0 5 19.832881662149955 22.0 4.0 30.0 4.0 33.0 6 12.817223727792834 10.0 4.0 15.0 4.0 30.0 7 18.9551339957844 22.0 4.0 29.0 4.0 33.0 8 20.13791026799157 24.0 4.0 31.0 4.0 34.0 9 19.40258958145137 23.0 4.0 29.0 4.0 33.0 10 16.34206564287865 17.0 4.0 25.0 4.0 30.0 11 17.870219813309244 19.0 4.0 28.0 4.0 33.0 12 20.1869918699187 24.0 4.0 32.0 4.0 35.0 13 20.378801565793434 24.0 4.0 32.0 4.0 35.0 14 19.683529057512796 23.0 4.0 31.0 4.0 35.0 15 19.101174345076785 22.0 4.0 31.0 4.0 35.0 16 17.062631737428486 17.0 4.0 29.0 4.0 34.0 17 18.128274616079494 20.0 4.0 31.0 4.0 35.0 18 18.15989159891599 20.0 4.0 31.0 4.0 35.0 19 15.862691960252937 12.0 4.0 28.0 4.0 33.0 20 16.82324601023788 16.0 4.0 30.0 4.0 34.0 21 14.42186088527552 10.0 4.0 26.0 4.0 33.0 22 15.432700993676603 9.0 4.0 28.0 4.0 33.0 23 15.047274917193617 8.0 4.0 28.0 4.0 33.0 24 14.672086720867208 4.0 4.0 27.0 4.0 33.0 25 14.775971093044264 4.0 4.0 28.0 4.0 33.0 26 13.324902137910268 4.0 4.0 25.0 4.0 33.0 27 13.36916591388136 4.0 4.0 26.0 4.0 33.0 28 13.219813309244204 4.0 4.0 26.0 4.0 33.0 29 12.202047576031315 4.0 4.0 22.0 4.0 32.0 30 11.91087021981331 4.0 4.0 22.0 4.0 32.0 31 11.597109304426377 4.0 4.0 23.0 4.0 32.0 32 10.877145438121048 4.0 4.0 18.0 4.0 32.0 33 9.928635953026197 4.0 4.0 13.0 4.0 30.0 34 9.557061126166817 4.0 4.0 10.0 4.0 30.0 35 9.133393556157785 4.0 4.0 4.0 4.0 30.0 36 8.88888888888889 4.0 4.0 4.0 4.0 30.0 37 8.884372177055104 4.0 4.0 4.0 4.0 30.0 38 8.645889792231255 4.0 4.0 4.0 4.0 30.0 39 8.49292381812707 4.0 4.0 4.0 4.0 29.0 40 8.164709424872026 4.0 4.0 4.0 4.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.5375448028673837 1 2 7.5219491380781704 1 3 -5.425840587130912 1 4 5.781549752517499 1 5 6.352811913295785 1 6 6.9826591568527085 1 7 6.02683905103261 1 8 2.5422896398702797 1 9 0.4747439836149496 1 10 0.7755760368663598 1 11 2.573873527905789 1 12 -0.01125618706263154 1 13 -6.954753370882406 1 14 2.116764806280937 1 15 -5.945118620925069 1 16 0.3091227171872326 1 17 -1.7096475507765803 1 18 0.7927590032428746 1 19 -2.1664021164021126 1 20 -1.5782386072708654 1 21 -1.0393283836832214 1 22 2.6179723502304117 1 23 1.5185057176992647 1 24 -0.09400494965011141 1 25 5.564038231780165 1 26 3.3317844342037883 1 27 6.040663935825229 1 28 1.7725721112817876 1 29 7.364025430960915 1 30 6.5505589691073585 1 31 8.197081413210444 1 32 7.5604241338112335 1 33 9.942140296979005 1 34 5.205111793821468 1 35 -0.07386072708653302 1 36 2.880077658303465 1 37 9.012766683734426 1 38 4.927346816862945 1 39 8.155871309097115 1 40 3.0412997098480963 6 1 4.204211469534052 6 2 4.236234852363886 6 3 0.6217784604881338 6 4 -2.647021676053928 6 5 3.9718595323434016 6 6 4.839802013995566 6 7 4.169696193889752 6 8 -1.743424645844005 6 9 7.665220174091139 6 10 1.0136712749616006 6 11 4.145302099334362 6 12 9.274458098651653 6 13 10.426199010069976 6 14 9.354860044376178 6 15 4.912024236217789 6 16 0.4995989076634224 6 17 6.29035244922342 6 18 10.935616146100017 6 19 13.214550264550269 6 20 5.897951868919609 6 21 8.198766854412018 6 22 10.713210445468507 6 23 16.66136286055641 6 24 16.334566478921317 6 25 15.897371565113497 6 26 13.28416538658474 6 27 18.564473459634755 6 28 17.867810206519884 6 29 15.173549240484725 6 30 13.312463731012118 6 31 22.101843317972353 6 32 24.893757467144567 6 33 20.32309267793139 6 34 18.490826079535754 6 35 24.21185355862775 6 36 15.546744324970133 6 37 19.869909540877284 6 38 13.070203959720088 6 39 19.63206178528759 6 40 16.803204471752856 7 1 5.037544802867384 7 2 8.861234852363886 7 3 5.788445127154802 7 4 11.977978323946072 7 5 7.0135261990100695 7 6 12.589802013995566 7 7 -1.8303038061102477 7 8 -3.743424645844005 7 9 -1.0431131592421927 7 10 3.7220046082949327 7 11 8.020302099334362 7 12 5.774458098651653 7 13 5.134532343403308 7 14 10.06319337770951 7 15 11.287024236217789 7 16 9.29126557433009 7 17 7.41535244922342 7 18 10.185616146100017 7 19 12.297883597883601 7 20 10.564618535586277 7 21 9.53210018774535 7 22 9.796543778801839 7 23 7.661362860556407 7 24 7.459566478921317 7 25 10.814038231780165 7 26 6.992498719918073 7 27 7.147806792968087 7 28 8.451143539853216 7 29 10.381882573818057 7 30 9.354130397678786 7 31 8.018509984639017 7 32 9.060424133811233 7 33 3.9064260112647204 7 34 8.240826079535754 7 35 1.9618535586277535 7 36 0.7550776583034651 7 37 0.4949095408772841 7 38 0.6952039597200876 7 39 1.1737284519542586 7 40 1.219871138419525 8 1 9.204211469534048 8 2 8.902901519030554 8 3 10.621778460488134 8 4 5.01964499061274 8 5 4.971859532343402 8 6 23.50646868066223 8 7 15.169696193889752 8 8 13.256575354155995 8 9 14.331886840757807 8 10 8.347004608294933 8 11 12.145302099334362 8 12 12.607791431984989 8 13 10.09286567673664 8 14 14.354860044376174 8 15 3.245357569551121 8 16 10.499598907663422 8 17 5.957019115890088 8 18 7.602282812766685 8 19 12.214550264550269 8 20 11.231285202252945 8 21 7.865433521078682 8 22 15.379877112135171 8 23 11.99469619388974 8 24 17.001233145587985 8 25 9.230704898446833 8 26 5.284165386584741 8 27 0.23114012630142078 8 28 -0.7988564601467836 8 29 1.840215907151391 8 30 1.9791303976787855 8 31 7.435176651305685 8 32 6.227090800477901 8 33 9.65642601126472 8 34 7.490826079535754 8 35 9.545186891961086 8 36 10.213410991636797 8 37 5.203242874210618 8 38 8.736870626386755 8 39 14.63206178528759 8 40 14.803204471752856 9 1 8.537544802867384 9 2 15.236234852363886 9 3 13.288445127154802 9 4 7.352978323946072 9 5 13.63852619901007 9 6 4.839802013995566 9 7 11.169696193889752 9 8 10.256575354155995 9 9 9.331886840757807 9 10 17.34700460829493 9 11 18.145302099334362 9 12 9.274458098651653 9 13 14.759532343403308 9 14 18.68819337770951 9 15 16.91202423621779 9 16 15.16626557433009 9 17 18.29035244922342 9 18 20.935616146100017 9 19 -3.452116402116399 9 20 19.564618535586277 9 21 24.53210018774535 9 22 24.04654377880184 9 23 15.661362860556407 9 24 16.334566478921317 9 25 13.564038231780165 9 26 26.617498719918075 9 27 26.897806792968087 9 28 25.201143539853216 9 29 23.506882573818057 9 30 28.979130397678787 9 31 16.768509984639017 9 32 17.56042413381123 9 33 3.6564260112647204 9 34 20.490826079535754 9 35 -4.7881464413722465 9 36 -4.119922341696535 9 37 -4.130090459122716 9 38 -3.9297960402799124 9 39 -3.7012715480457414 9 40 -3.530128861580475 10 1 10.537544802867384 10 2 13.236234852363886 10 3 -1.7115548728451984 10 4 17.352978323946072 10 5 15.63852619901007 10 6 20.839802013995566 10 7 16.169696193889752 10 8 15.256575354155995 10 9 15.331886840757807 10 10 11.347004608294933 10 11 15.145302099334362 10 12 15.274458098651653 10 13 12.759532343403308 10 14 15.68819337770951 10 15 10.912024236217789 10 16 -12.83373442566991 10 17 -13.70964755077658 10 18 -13.064383853899983 10 19 -10.452116402116399 10 20 -12.435381464413723 10 21 -9.46789981225465 10 22 -9.953456221198161 10 23 -10.338637139443593 10 24 -9.665433521078683 10 25 -10.435961768219835 10 26 -9.382501280081927 10 27 -9.102193207031913 10 28 -8.798856460146784 10 29 -7.493117426181943 10 30 -7.0208696023212145 10 31 -7.231490015360983 10 32 -6.4395758661887665 10 33 -5.34357398873528 10 34 -5.509173920464246 10 35 -4.7881464413722465 10 36 -4.119922341696535 10 37 -4.130090459122716 10 38 -3.9297960402799124 10 39 -3.7012715480457414 10 40 -3.530128861580475 12 1 5.037544802867384 12 2 4.236234852363886 12 3 1.6217784604881338 12 4 -2.647021676053928 12 5 3.4718595323434016 12 6 -0.8268646526710999 12 7 1.8363628605564202 12 8 6.256575354155995 12 9 2.998553507424475 12 10 7.180337941628265 12 11 11.478635432667694 12 12 8.441124765318321 12 13 7.759532343403308 12 14 11.521526711042842 12 15 13.078690902884453 12 16 16.499598907663426 12 17 15.457019115890084 12 18 15.268949479433353 12 19 11.381216931216933 12 20 19.23128520225294 12 21 15.365433521078682 12 22 18.379877112135176 12 23 14.99469619388974 12 24 18.834566478921317 12 25 12.897371565113497 12 26 8.450832053251405 12 27 0.8978067929680869 12 28 10.367810206519884 12 29 7.506882573818057 12 30 6.812463731012118 12 31 0.4351766513056834 12 32 4.393757467144567 12 33 1.3230926779313865 12 34 1.9908260795357542 12 35 5.545186891961087 12 36 2.046744324970131 12 37 1.3699095408772841 12 38 -0.9297960402799124 12 39 -0.03460488137907447 12 40 0.9698711384195251 13 1 -4.962455197132616 13 2 1.7362348523638858 13 3 -2.2115548728451984 13 4 1.852978323946072 13 5 0.13852619901006946 13 6 3.839802013995566 13 7 0.6696961938897523 13 8 1.256575354155995 13 9 1.3318868407578073 13 10 5.347004608294933 13 11 1.6453020993343621 13 12 -1.2255419013483468 13 13 0.7595323434033077 13 14 -3.81180662229049 13 15 1.912024236217789 13 16 4.16626557433009 13 17 3.2903524492234197 13 18 2.435616146100017 13 19 6.547883597883601 13 20 4.564618535586277 13 21 7.53210018774535 13 22 7.046543778801839 13 23 6.661362860556407 13 24 0.3345664789213174 13 25 6.564038231780165 13 26 7.617498719918073 13 27 7.897806792968087 13 28 8.201143539853216 13 29 9.506882573818057 13 30 -0.020869602321214487 13 31 4.768509984639017 13 32 5.0604241338112335 13 33 5.15642601126472 13 34 -0.00917392046424581 13 35 7.2118535586277535 13 36 7.880077658303465 13 37 10.369909540877284 13 38 5.070203959720088 13 39 6.798728451954259 13 40 10.969871138419524 16 1 5.537544802867384 16 2 2.2362348523638858 16 3 6.621778460488134 16 4 14.352978323946072 16 5 7.6385261990100695 16 6 1.1731353473289001 16 7 6.83636286055642 16 8 9.923242020822663 16 9 9.66522017409114 16 10 9.347004608294933 16 11 13.478635432667694 16 12 8.941124765318321 16 13 9.759532343403308 16 14 9.68819337770951 16 15 6.245357569551121 16 16 9.832932240996758 16 17 16.29035244922342 16 18 15.935616146100017 16 19 15.881216931216933 16 20 16.897951868919613 16 21 21.865433521078685 16 22 11.046543778801839 16 23 22.994696193889745 16 24 23.334566478921317 16 25 21.23070489844683 16 26 20.617498719918075 16 27 20.56447345963475 16 28 8.534476873186549 16 29 1.840215907151391 16 30 3.3124637310121194 16 31 -1.8981566820276488 16 32 2.5604241338112335 16 33 3.6564260112647204 16 34 3.490826079535754 16 35 3.8785202252944195 16 36 4.880077658303465 16 37 3.203242874210618 16 38 4.736870626386754 16 39 -3.7012715480457414 16 40 -3.530128861580475 17 1 5.537544802867384 17 2 7.3473459634749965 17 3 7.510667349377023 17 4 7.464089435057183 17 5 8.74963731012118 17 6 8.062024236217788 17 7 8.614140638334195 17 8 10.367686465267106 17 9 8.998553507424475 17 10 10.680337941628265 17 11 10.478635432667694 17 12 8.385569209762764 17 13 9.20397678784775 17 14 10.465971155487289 17 15 8.800913125106678 17 16 4.277376685441201 17 17 7.512574671445641 17 18 6.824505034988906 17 19 10.32566137566138 17 20 6.897951868919609 17 21 4.976544632189793 17 22 7.379877112135171 17 23 7.439140638334186 17 24 8.556788701143539 17 25 4.675149342891276 17 26 -3.049167946748593 17 27 5.008917904079198 17 28 3.423365762075438 17 29 1.9513270182625018 17 30 3.756908175456564 17 31 6.1018433179723495 17 32 7.449313022700123 17 33 6.65642601126472 17 34 5.713048301757976 17 35 6.2118535586277535 17 36 6.32452210274791 17 37 6.4254650964328395 17 38 8.959092848608977 17 39 7.076506229732037 17 40 7.6920933606417465 18 1 7.162544802867384 18 2 3.2362348523638858 18 3 4.288445127154802 18 4 12.852978323946072 18 5 11.76352619901007 18 6 10.589802013995566 18 7 10.294696193889752 18 8 12.631575354155995 18 9 9.706886840757807 18 10 11.472004608294933 18 11 3.645302099334362 18 12 9.899458098651653 18 13 11.259532343403308 18 14 9.81319337770951 18 15 9.037024236217789 18 16 -1.0837344256699097 18 17 4.29035244922342 18 18 7.310616146100017 18 19 7.922883597883601 18 20 0.564618535586277 18 21 2.9071001877453497 18 22 6.921543778801839 18 23 9.286362860556407 18 24 8.459566478921317 18 25 5.314038231780165 18 26 4.617498719918073 18 27 4.647806792968087 18 28 6.576143539853216 18 29 4.381882573818057 18 30 6.6041303976787855 18 31 10.018509984639017 18 32 3.0604241338112335 18 33 4.03142601126472 18 34 3.365826079535754 18 35 6.0868535586277535 18 36 3.755077658303465 18 37 0.11990954087728412 18 38 -0.05479604027991236 18 39 0.17372845195425857 18 40 0.3448711384195251 19 1 10.537544802867384 19 2 9.569568185697218 19 3 13.288445127154802 19 4 6.352978323946072 19 5 15.63852619901007 19 6 13.839802013995566 19 7 9.503029527223084 19 8 13.92324202082266 19 9 8.66522017409114 19 10 17.680337941628267 19 11 9.145302099334362 19 12 14.607791431984989 19 13 15.759532343403308 19 14 8.021526711042842 19 15 14.578690902884453 19 16 11.499598907663422 19 17 18.29035244922342 19 18 15.602282812766681 19 19 16.5478835978836 19 20 18.23128520225294 19 21 13.53210018774535 19 22 10.713210445468507 19 23 1.661362860556407 19 24 12.334566478921317 19 25 17.23070489844683 19 26 14.28416538658474 19 27 13.897806792968087 19 28 7.201143539853216 19 29 9.173549240484725 19 30 -0.020869602321214487 19 31 0.10184331797235124 19 32 2.2270908004778995 19 33 -5.34357398873528 19 34 -5.509173920464246 19 35 -4.7881464413722465 19 36 -4.119922341696535 19 37 -4.130090459122716 19 38 -3.9297960402799124 19 39 -3.7012715480457414 19 40 -3.530128861580475 20 1 5.037544802867384 20 2 1.2362348523638858 20 3 7.788445127154802 20 4 -3.647021676053928 20 5 1.6385261990100695 20 6 -0.6601979860044338 20 7 0.6696961938897523 20 8 -1.743424645844005 20 9 -6.668113159242193 20 10 9.347004608294933 20 11 2.645302099334362 20 12 4.774458098651653 20 13 5.259532343403308 20 14 8.18819337770951 20 15 8.412024236217789 20 16 8.16626557433009 20 17 13.29035244922342 20 18 10.435616146100017 20 19 5.047883597883601 20 20 17.564618535586277 20 21 -1.4678998122546503 20 22 4.546543778801839 20 23 2.661362860556407 20 24 -7.665433521078683 20 25 1.0640382317801649 20 26 -4.382501280081927 20 27 4.397806792968087 20 28 -5.298856460146784 20 29 5.506882573818057 20 30 6.4791303976787855 20 31 -4.231490015360983 20 32 7.0604241338112335 20 33 8.15642601126472 20 34 7.990826079535754 20 35 5.2118535586277535 20 36 11.380077658303465 20 37 6.369909540877284 20 38 11.570203959720088 20 39 3.2987284519542586 20 40 3.469871138419525 25 1 8.37087813620072 25 2 8.069568185697218 25 3 9.45511179382147 25 4 7.852978323946072 25 5 7.971859532343402 25 6 4.506468680662234 25 7 11.669696193889752 25 8 2.923242020822663 25 9 0.6652201740911394 25 10 6.680337941628265 25 11 9.478635432667694 25 12 13.274458098651653 25 13 2.9261990100699755 25 14 4.521526711042842 25 15 13.578690902884453 25 16 5.66626557433009 25 17 3.4570191158900876 25 18 8.435616146100017 25 19 9.547883597883601 25 20 8.397951868919609 25 21 3.365433521078682 25 22 12.213210445468507 25 23 4.161362860556407 25 24 2.5012331455879853 25 25 3.064038231780165 25 26 4.284165386584741 25 27 6.897806792968087 25 28 6.867810206519884 25 29 7.673549240484725 25 30 8.145797064345453 25 31 8.768509984639017 25 32 10.227090800477901 25 33 5.15642601126472 25 34 9.990826079535754 25 35 5.8785202252944195 25 36 4.046744324970131 25 37 2.369909540877284 25 38 5.070203959720088 25 39 2.9653951186209246 25 40 2.303204471752859 26 1 7.037544802867384 26 2 6.611234852363886 26 3 0.7884451271548016 26 4 13.227978323946072 26 5 10.01352619901007 26 6 1.0898020139955662 26 7 7.044696193889752 26 8 10.006575354155995 26 9 9.206886840757807 26 10 7.847004608294933 26 11 10.895302099334362 26 12 8.899458098651653 26 13 13.384532343403308 26 14 12.06319337770951 26 15 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7 20.0 8 19.0 9 37.0 10 85.0 11 103.0 12 115.0 13 113.0 14 115.0 15 106.0 16 125.0 17 113.0 18 146.0 19 129.0 20 136.0 21 153.0 22 134.0 23 162.0 24 138.0 25 100.0 26 103.0 27 67.0 28 61.0 29 35.0 30 23.0 31 12.0 32 10.0 33 4.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 93.58240433865622 5.60409761976499 0.0903886712865321 0.7231093702922567 8 97.77108433734941 0.5421686746987951 0.5120481927710844 1.1746987951807228 9 97.62048192771084 1.5963855421686748 0.4518072289156626 0.3313253012048193 10 60.82505269497139 24.570912375790424 5.751279735019573 8.85275519421861 11 14.062029509183983 58.20535983137609 17.46461909063535 10.267991568804577 12 23.102409638554217 10.512048192771084 53.01204819277109 13.373493975903614 13 55.37488708220416 14.212586570310147 15.92893706714845 14.483589280337247 14 14.423366455886782 55.796446853357416 15.65793435712135 14.122252333634448 15 55.37488708220416 17.19361638060825 14.875037639265281 12.556458897922312 16 15.176151761517614 51.70129479072568 16.832279433905452 16.29027401385125 17 16.350496838301716 14.062029509183983 54.89310448660042 14.694369165913882 18 56.278229448961156 14.152363745859681 15.176151761517614 14.393255043661549 19 42.615012106537534 10.865617433414043 12.5 34.01937046004843 20 15.236374585968083 51.30984643179766 15.356820234869014 18.096958747365253 21 14.51370069256248 13.128575730201748 19.391749473050286 52.965974104185484 22 11.38211382113821 13.33935561577838 53.35742246311352 21.92110809996989 23 13.730803974706413 16.320385426076484 36.3745859680819 33.5742246311352 24 11.653116531165312 46.612466124661246 17.52484191508582 24.209575429087625 25 14.074743821579263 52.41109101868595 15.822784810126583 17.6913803496082 26 13.317191283292978 48.60774818401937 18.734866828087167 19.340193704600484 27 32.24932249322493 13.399578440228845 36.70581150255947 17.64528756398675 28 48.07228915662651 15.873493975903614 18.94578313253012 17.10843373493976 29 10.497737556561086 50.13574660633484 21.116138763197586 18.250377073906485 30 9.795057263411694 44.99698613622664 25.01506931886679 20.192887281494876 31 9.008737571557699 14.040373606507984 62.63934920156674 14.311539620367581 32 12.707015959048478 10.870219813309244 61.06594399277326 15.356820234869014 33 42.77108433734939 14.096385542168674 27.92168674698795 15.210843373493976 34 9.30442637759711 43.23998795543511 27.732610659439928 19.722975007527854 35 11.261668172237279 11.050888286660644 26.648599819331526 51.038843721770554 36 42.60764829870521 12.195121951219512 27.551942186088528 17.64528756398675 37 46.265060240963855 11.114457831325302 27.7710843373494 14.849397590361447 38 42.908762420957544 10.20776874435411 30.773863294188498 16.10960554049985 39 10.087323095453177 14.875037639265281 60.10237880156579 14.93526046371575 40 8.611863896416743 44.564890093345376 27.49171936163806 19.33152664859982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.0 19 1.0 20 0.0 21 5.5 22 11.0 23 11.0 24 14.0 25 17.0 26 17.5 27 18.0 28 18.0 29 31.0 30 44.0 31 45.5 32 47.0 33 47.0 34 49.0 35 51.0 36 61.0 37 71.0 38 71.0 39 88.5 40 106.0 41 123.0 42 140.0 43 140.0 44 158.0 45 176.0 46 261.5 47 347.0 48 347.0 49 433.5 50 520.0 51 511.5 52 503.0 53 503.0 54 433.5 55 364.0 56 293.5 57 223.0 58 223.0 59 190.0 60 157.0 61 146.0 62 135.0 63 135.0 64 120.5 65 106.0 66 104.5 67 103.0 68 103.0 69 84.5 70 66.0 71 59.5 72 53.0 73 53.0 74 41.5 75 30.0 76 22.0 77 14.0 78 14.0 79 13.0 80 12.0 81 6.5 82 1.0 83 1.0 84 1.0 85 1.0 86 1.0 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06022282445046673 8 0.030111412225233364 9 0.030111412225233364 10 0.0 11 0.0 12 0.030111412225233364 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5118940078289671 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.09033423667570009 26 0.5118940078289671 27 0.0 28 0.030111412225233364 29 0.18066847335140018 30 0.09033423667570009 31 0.06022282445046673 32 0.0 33 0.030111412225233364 34 0.0 35 0.0 36 0.0 37 0.030111412225233364 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 3321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.38000602228244 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23741836342681 73.86329418849743 2 3.11179408374952 4.878048780487805 3 0.8067614291202458 1.8970189701897018 4 0.42258932001536686 1.324902137910268 5 0.23050326546292738 0.9033423667570009 6 0.2689204763734153 1.2646793134598013 7 0.1920860545524395 1.0538994278831677 8 0.11525163273146369 0.7226738934056007 9 0.0384172109104879 0.27100271002710025 >10 0.42258932001536686 4.938271604938271 >50 0.1536688436419516 8.882866606443843 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA 91 2.740138512496236 No Hit GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA 70 2.1077988557663354 No Hit GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA 67 2.0174646190906356 No Hit GAACGCGGGGATGAGATGGACTCAAATGAATTGACGGGTA 67 2.0174646190906356 No Hit GAACGCGGGGATGAGATGGACTCCAAGGAATTGACGGGTA 23 0.6925624811803673 No Hit GAACGCGGGGATGAGATGCACTTAAATGAATTGACGGGTA 18 0.5420054200542005 No Hit GAACGCGGGGATGAGATGCACTTAAAGGAATTGACGGGTA 17 0.5118940078289671 No Hit GAACGCGGGCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT 15 0.45167118337850043 No Hit GAACGCGGGGATGAGATGGACTTCAAGGAATTGACGGGTA 15 0.45167118337850043 No Hit GAACGCGGGGATGAGATGCACTCAAATGAATTGACGGGTA 14 0.4215597711532671 No Hit GAACGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTA 13 0.3914483589280337 No Hit GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTA 13 0.3914483589280337 No Hit GAACGCGGGGATGAGATGGACTTCAATGAATTGACGGGTA 13 0.3914483589280337 No Hit GAACGCGGGGATGAGATGCACTCAAAGGAATTGACGGGTA 12 0.36133694670280037 No Hit GAACGCGGGGATGAGATGGACTCCAATGAATTGACGGGTA 11 0.331225534477567 No Hit GAACGCGGGGATGAGATGGACTTAACGGAATTGACGGGTA 9 0.27100271002710025 No Hit GAACGCGGGATGAGATGGACTCAAATGAATTGACGGGTAC 8 0.2408912978018669 No Hit GAACGCGGGGATGAGATGGACTCACATGAATTGACGGGTA 8 0.2408912978018669 No Hit GAACGCGGGGATGAGATGGACTCAAATGAATTGACCGGTA 8 0.2408912978018669 No Hit GAACGCGGGGATGAGATGGACTTAAAGGACTTGACGGGTA 7 0.21077988557663355 No Hit GAACGCGGGGATGAGATGGACTCAAAGGAATTGCCGGGTA 7 0.21077988557663355 No Hit GAACGCAGGGATGAGATGGACTTAAATGAATTGACGGGTA 7 0.21077988557663355 No Hit GAACGCGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 7 0.21077988557663355 No Hit GAACGCGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 7 0.21077988557663355 No Hit GAACGCGGGGATGAGATGGACTTACATGAATTGACGGGTA 6 0.18066847335140018 No Hit GAACGCGGGGATGAGATGGACTCAACGGAATTGACGGGTA 6 0.18066847335140018 No Hit GAACGCAGGGATGAGATGGACTCAAATGAATTGACGGGTA 6 0.18066847335140018 No Hit GAACGCGGGATGAGATGGCCTTAAATGAATTGACGGGTAC 6 0.18066847335140018 No Hit GAACGCGGGATGAGATGGACTTAAATGAATTGACGGGTAC 6 0.18066847335140018 No Hit GAACGCGGGGATGAGATGGACTCAACTGAATTGACGGGTA 6 0.18066847335140018 No Hit GAACGCGGGGATGAGATGGACTTACAGGAATTGACGGGTA 6 0.18066847335140018 No Hit GAACGCAGGGATGAGATGCACTTAAATGAATTGACGGGTA 5 0.15055706112616682 No Hit GAACGCGGGGATGAGATGGACTCAAAGGACTTGACGGGTA 5 0.15055706112616682 No Hit GAACGCAGGGATGAGATGCACTTAAAGGAATTGACGGGTA 5 0.15055706112616682 No Hit GAACGCGGGGTACGGGAATGTAGGAACGTCCCTGTCTTGT 5 0.15055706112616682 No Hit GAACGCGGGGATGAGATGGACTTAAAGGAATTGACCGGTA 5 0.15055706112616682 No Hit GAACGCGGGCAGAGTCGTTTTTTTTTTTTTTTTTTTTTTT 5 0.15055706112616682 No Hit GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTC 4 0.12044564890093346 No Hit GAACGCGGGGATGAGATGGACTTAAATGAATTGACCGGTA 4 0.12044564890093346 No Hit GAACGCGGGGATGAGATGGACCTAAAGGAATTGACGGGTA 4 0.12044564890093346 No Hit GAACGCGGGCTCGTTTTTCTTTTTTTCTTTTTTTTTTTTC 4 0.12044564890093346 No Hit GAACGCGGGCTGTAGCTTTGGCACGTCCCGAGTTCTTTGT 4 0.12044564890093346 No Hit GAACGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 4 0.12044564890093346 No Hit GAACGCGGGGATGAGATGCACTCAAAGGACTTGACGGGTA 4 0.12044564890093346 No Hit GAACGCGGGGATGAGATGGACCCAAAGGAATTGACGGGTA 4 0.12044564890093346 No Hit GAACGCGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 4 0.12044564890093346 No Hit GAACGCGGGGATGAGCTGGACTCAAAGGAATTGACGGGTA 4 0.12044564890093346 No Hit GAACGCAGGGATGAGATGGACTCCAATGAATTGACGGGTA 4 0.12044564890093346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGGGG 20 0.0023331598 34.2 5 GCGGGGA 170 0.0 34.2 5 GCGGGAT 25 1.5638264E-4 34.2 5 AACGCGG 310 0.0 34.2 2 TAAATGA 25 1.5638264E-4 34.2 23 GGACTTA 30 1.0378535E-5 34.2 18 GGACTCC 20 0.0023331598 34.2 18 AGGAATT 35 6.814553E-7 34.2 26 GAACGCG 320 0.0 34.2 1 TCAAAGG 25 1.5638264E-4 34.2 22 CGGGATG 20 0.0023331598 34.2 6 ACTTAAA 25 1.5638264E-4 34.2 20 AAAGGAA 30 1.0378535E-5 34.2 24 TGGACTT 35 6.814553E-7 34.2 17 CAAAGGA 20 0.0023331598 34.2 23 CGCGGGT 35 6.814553E-7 34.2 4 CGCGGGC 20 0.0023331598 34.2 4 CTTAAAT 20 0.0023331598 34.2 21 AAGGAAT 30 1.0378535E-5 34.2 25 ACGCGGG 305 0.0 34.2 3 >>END_MODULE