FastQCFastQC Report
Fri 17 Jun 2016
SRR1525972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA21.342281879194631No Hit
GAACGCGGGTACGATCGCCTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGAACCTGGTACACCCAGAGCCGCCTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGACGGGTA10.6711409395973155No Hit
GAACGCGGTGTATCACAGAGTCGAGTTTTTCTTTTTTATT10.6711409395973155No Hit
GAACGCGGGGCTCCGAGACTTATACCGCAGCTCTCCCCGT10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTANAGGAATTGACGGGTA10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACCCAAATGACTTGACGGGTA10.6711409395973155No Hit
GAACGCGGGAGCAGGAAAGAGAAGACCTCACACCGGAACA10.6711409395973155No Hit
GAACGCGGGGGTCCAGATCGACTTTCTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGAAGCAGTTATCCCAAACAAAACACCCAAACC10.6711409395973155No Hit
GAACGCGGGCTGGCTTCAGCCATACTCCCACCCCCACTGC10.6711409395973155No Hit
GAACGCGGGGCTGAGATGGGAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GAACGCGGGGCTGAGATGGACTTAAAGGAATTGACGGGTA10.6711409395973155No Hit
GAACGCGGGGAAGCACTGGACTCAACGCAACTGACGGCTC10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTAAATGAATTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGATGTATAAAAAAAAACAAAAACACAACACCA10.6711409395973155No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGCG10.6711409395973155No Hit
GAACGCGGGCCAGCTACTTTGTCTACCTACTTGGTTCCCG10.6711409395973155No Hit
GAACGCGGGATCTGTGGACAAACCAGTACCTGCGCCAGAA10.6711409395973155No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGAAGTTCTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCAGGGATGAGATGGACTTAAAGGAATTGACGGACA10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTACAGGAATACCCGGTAC10.6711409395973155No Hit
GAACGCGGGGGAAAAAAAAAAAAAACCAAACACACAACAC10.6711409395973155No Hit
GAACGCGGGATTAAAAAACTTGTCTCCCCCCACCAAACCT10.6711409395973155No Hit
GAACGCGGGGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGTAACTTCCAACCCAGAGCCGCCTTTTTTTTTT10.6711409395973155No Hit
GAACGCAGGGACGACCACCGCGAACCCACATCTTCCTCTG10.6711409395973155No Hit
GAACGCGGGAAGCCGTGGTATCAACCCCGAGTCGACTTTT10.6711409395973155No Hit
GAACGCGGGATATTTGTTTCCAACAATGGTGCCTCTGCGT10.6711409395973155No Hit
GAACGCGGGGACCTTTTTTTTTTTTTTTTTTTTTTTTTTC10.6711409395973155No Hit
GAACGCGGGGATGAGATGGCGAAANACAACAAAAAACAAA10.6711409395973155No Hit
GAACGCGGGACCTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.6711409395973155No Hit
GAACGCGGGAGTGTACCAGAGTCGATNTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGCGAAATAACAACTCCTTCCCCACAACACCACCG10.6711409395973155No Hit
GAACGCGGGTTTCAACCAGATCGAACCACCCCCCCCACCC10.6711409395973155No Hit
GAACGCGGGAAGTACAACCCAGAGCCGACCTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCGTCGATTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGCTTTTTAAAGTTAAAAGAACCACATGAAAGAC10.6711409395973155No Hit
GAACGCGGGGGGGCCCCGAGAGATGGCCCACCGGTTCCGC10.6711409395973155No Hit
GAACGCGGGAATACCAGATCGACTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGGCCCCCCCCGCCCACCCCCCGCCCCCCCCC10.6711409395973155No Hit
GAACGCGGGTGGTACGAGCGTCGTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCCTGTGTTACCTGTCTGTCTGCCCTCGTGCC10.6711409395973155No Hit
GAACGCGGGACTTAAACGCAGAGTCGACCTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGATGAGATGGCGAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GAACGCGGGGGGCGTGCAGTTGCGGTACCTACTAGTGAGT10.6711409395973155No Hit
GAACGCCGGCCGGTGGCCACCCCAGCGCCCCCTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGGGCTGGAGAGATGGCCCAGTAGTTACGACC10.6711409395973155No Hit
GAACGCGGGATAGCTTTGGCTTCTTTGTGGCTCCCGACCC10.6711409395973155No Hit
GAACGCGGGTTGCATTCCAAAAAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GAACGCGGGACTGGTCAACCCAGAGCCGCCCTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCATGGTACAACCCAAAAAAAAAAAAAAAAAA10.6711409395973155No Hit
GAACGCGGGGATGCGATGGACAAAAACAAACACAAAAAAC10.6711409395973155No Hit
GAACGCGGGAAATCCCAGTCGCTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCAGTAAAATTCATTTTTTTTTTGCTTTTGTT10.6711409395973155No Hit
GAACGCGGGACCCCGGTGACCTTGGGATTATTTTTTTAAA10.6711409395973155No Hit
GAACGCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGATGGCAACCTTGAGCTTCTCCCCCCCCCCCC10.6711409395973155No Hit
GAACGCGGGACGCCGCGATTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGATGAGCTGGACTTAAATGAATTGCCGGTCAA10.6711409395973155No Hit
GAACGCGGGGATGAGATGCATCGATGTGTTAGATGCGCTG10.6711409395973155No Hit
GAACGCGGGAGTTCTGGCCGGTCCCTGTAGGTTGATCTCT10.6711409395973155No Hit
GAACGCGGGGATGAGATGGGAAAAACCAAACACCAAAAAA10.6711409395973155No Hit
GAACGCGGGTGTCACGCCGAGTCGCCTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGTGACTGGTTATACGCCCCCCCTAATACTCT10.6711409395973155No Hit
GAACGCGGTGGTCAACCAGAGTGACCTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGACCGTTTTTCCTTTAAAAATTTTACGTGCGT10.6711409395973155No Hit
GAACGCGGGAAGCCAACGAGAGTCTTTTTTTTTTTCTTTT10.6711409395973155No Hit
GAACGCGGGCCCGCCTCCCCCCCCCCCCCACCCGCCCCGC10.6711409395973155No Hit
GAACGCGGGAAGCAGTCCAACAGAGCCGACCTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCATGAGCTGCCCTTAAATGACTTGACGGGTA10.6711409395973155No Hit
GAACGCGGGACTGAGACCTTGGTTGAGGACTGGCACTTTT10.6711409395973155No Hit
GAACGCGGGAAGCCTCGTCCCACCCCTACTCTTAAACCTC10.6711409395973155No Hit
GAACGCGGGGTGTGCTGGTTTCCATGACTCCCGCGCAGCC10.6711409395973155No Hit
GAACGCGGGCAGCATATCACCCCCGCGCCGCCTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGTGCAGTCCTGAACGGGTAATGAACCGCCCA10.6711409395973155No Hit
GAACGCGGGGTTCTTTGGGGGCATTTTTTTTTTTAATGGC10.6711409395973155No Hit
GAACGCGGGTAGTGGGGTTTTTAAACACTTGGAAACCAAA10.6711409395973155No Hit
GAACGCGGGATTGAAACTGTTTGTCCTTTGATCATAGTGT10.6711409395973155No Hit
GAACGCGGGGGGCTGGTGAGAAAGCTCCGTGGGTCAGAGC10.6711409395973155No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTCCGAGC10.6711409395973155No Hit
GAACGCTGGTCGACTTTTTCTTATTCCCCTATCCTGCTCC10.6711409395973155No Hit
GAACGCGGGCAAGTCGCTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGATGAGATGGCCTTAACGGACTTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGAAGCATGGTCAACCCGCCGACCTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGACATGGTTATAGTTAAAACATTTTTTCATGG10.6711409395973155No Hit
GAACGCGGGGATGAGCTGCCCCCAAATGAATTGACGGCCA10.6711409395973155No Hit
GAACGCGGGAATCGCGCGTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCNGGACGCAGTGTATCAACGCAGAGTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCTCGTCTTAATCAGCAACATCTTTTGACACA10.6711409395973155No Hit
GAACGCGGGGGCACCAAAACCAAAAAAAAAAAAAAACAAA10.6711409395973155No Hit
GAACGCAGGGATGAGATGCTCGGAAGACCTCGTATGCCGC10.6711409395973155No Hit
GAACGCGGGATAATTGCTAAGACTTTCCTATTTATTTTTT10.6711409395973155No Hit
GAACGCGGGGCTGAGATGGACCTACCCTTATCCCAGCCTC10.6711409395973155No Hit
GAACGCGGGGTCTGGCTAGCAGAGAGGGAAACGCCCTTGC10.6711409395973155No Hit
GAACGCGGGGGCCCCCCCCCCCTTCCGCCCCCACCTCCCC10.6711409395973155No Hit
GAACGCGGGAAGTCACGCAGAGTCGCCTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGATATCACGCAGGGTCGCCTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGACTCAAACTTCTGTGGCCACCCACTGATAGC10.6711409395973155No Hit
GAACGCGGGAAGCATGGTCAACGCCCCGCCCCCTTTTTCT10.6711409395973155No Hit
GAACGCGGGCACAACCAACCCCGACTTCTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCAGGCAGTGATCAAGAACGCAGCCCTGTCTG10.6711409395973155No Hit
GAACGCGGACATCCCGCAGAGTCGACTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGATGAGATGCCCCCAAATGACTCCCCGCCCC10.6711409395973155No Hit
GAACGCAGGGCTGAGATGGGGGGAGGGCATCCACAAACTA10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTCACGGAATTGACGGGTA10.6711409395973155No Hit
GAACGCNGGGCTGAGATGGACTCAAAGGAATTGACGGGTA10.6711409395973155No Hit
GAACGCGGGAAGCCGTGTCATCCACCATGCCATTACTCTA10.6711409395973155No Hit
GAACGCGGGTGCTGCTGAGCCCTTTCTGGATGTTGTCCTG10.6711409395973155No Hit
GAACGCGGCGGGTGCTTTATACCCAGGCCTGGGCCACCTA10.6711409395973155No Hit
GAACGCGGGGCTGAGATGGACCCAAATGAATTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGGAGATTAGACGGGCAACTTTGCCTGTGCAGT10.6711409395973155No Hit
GAACGCGGGACGAGCTGGACTTAAATGAATTGACGGGTAA10.6711409395973155No Hit
GAACGCGGGATATCCTGTTGTATTTGTTTCACTGCCCCCC10.6711409395973155No Hit
GAACGCAGGGATGAGATGGACTTAAAGGACTTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGGAGTCCATTTTTTTTTTTTTTTTTACCCGCA10.6711409395973155No Hit
GAACGCGGGAGATGTACAACCCAGAGCGACTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGATGAGATGCACCTAAAGCGGACTCCCTCCT10.6711409395973155No Hit
GAACGCGGGAAGCATGGTACCCCGCGCCGCCTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGCTCACGCAGACGATTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGAGGAAACATGGCGTTGTCCCTTTGGCCCCAT10.6711409395973155No Hit
GAACGCGGGATTTCCCCTCCAGATCTTACCCCCGTAAACC10.6711409395973155No Hit
GAACGCGGGTTCTCTACACTTGGTTTTTGTTACTGTTTTG10.6711409395973155No Hit
GAACGCGGGGATACGATAAACCCCACCCTCTTAACCAACC10.6711409395973155No Hit
GAACGCGGGGATGAGATCCACTTCAACGACTCGCCGCGTA10.6711409395973155No Hit
GAACGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGAGTCTGGAACTTGCTGTGTAAGCCTGGTCTT10.6711409395973155No Hit
GAACGCGGGGGGCTGGTCAGATGGCTCAGCGGGTCCGCGC10.6711409395973155No Hit
GAACGCGGGTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
GAACGCCGGGCTGAAATGGCCTTAAATGCCTTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGGACAGTAGTGTAGACAAAGCCCCCATGGGCA10.6711409395973155No Hit
GAACGCGGGACGCCGTCTCCACCAGATCGACTTTTTTTTT10.6711409395973155No Hit
GAACGCGGGGGGTTGGGGATTTAGCCCAGTGCTAGAGCCC10.6711409395973155No Hit
GAACGCAGGGATGAGATGCACCCAACTGACTTGACGGGTA10.6711409395973155No Hit
GAACGCGGGGATGAGATGCCCTTACAGCAATTCCCGGCCC10.6711409395973155No Hit
GAACGCCGGCCGCAGTGGTCTCAACCCCGCGCCGCCTTTT10.6711409395973155No Hit
GAACGCGGGGTGTAGTGCTGGCTTCCGTCCCGCTAGCCGC10.6711409395973155No Hit
GAACGCGGGAAGCAGTTTCAACGCCGAGCCGACTTTTTTT10.6711409395973155No Hit
GAACGCGGGGGAGGTGGTTGTAGCCCCTGCCCCCGGACCG10.6711409395973155No Hit
GAACGCGGGGATGAGATGCACTTAAAGGAATTGACGGGTC10.6711409395973155No Hit
GAACGCGGGCTGAGATGGACTTAAATGAATTGACGGGTAC10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA10.6711409395973155No Hit
GAACGCGGGGGCGTTCGTGGAGAACGACATGCCTCCCCTC10.6711409395973155No Hit
GAACGCGGGAAGTTGACAGAGGTGAAGGATGAGTTAGAAC10.6711409395973155No Hit
GAACGCGGGGATGAGATGGACTCAACGGAATTGCCGGGTC10.6711409395973155No Hit
GAACGCGGGCTGAGATGGACTCAAATGAATTGCCGGGTAC10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG100.034.02
GAACGCG100.034.01
ACGCGGG100.034.03