FastQCFastQC Report
Fri 17 Jun 2016
SRR1525970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37150
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2250.6056527590847914No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1670.4495289367429341No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1500.4037685060565276No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1100.2960969044414536No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG660.17765814266487212No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC640.17227456258411844No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC620.16689098250336473No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.15612382234185734No Hit
TCAAAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT490.13189771197846567No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC480.12920592193808883No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT400.10767160161507401No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA380.10228802153432032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA203.9509148E-450.4529340
TAAGACC150.00811719350.38419735
GTGCGCC150.00811719350.38419735
CCTCGAC150.00824938850.17910415
TAAGAAC150.00824938850.17910434
CCCTCGA150.00824938850.17910414
GATTTCC204.0860582E-450.111119
ATCTGTG150.00829380750.1111147
CACCATG150.00833840350.043349
GCACCAT150.00833840350.043348
CTCGACC150.00847326249.8409716
TCGACCA150.00847326249.8409717
TGCTGAT150.00847326249.8409730
CTCAGAT150.00847326249.8409716
CTCGTGT150.00847326249.8409729
AGGGGTA1050.049.773895
AGGGGCG355.29144E-849.773895
GGATGAA204.2248494E-449.7738888
GGATTTC252.1040074E-549.7738888
GCGGCCA150.00851857449.77388822
Produced by FastQC (version 0.11.5)