##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37150
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.586729475100945	39.0	37.0	39.0	31.0	40.0
2	36.00441453566622	39.0	36.0	39.0	29.0	40.0
3	34.257523553162855	38.0	35.0	39.0	17.0	40.0
4	33.04565275908479	38.0	31.0	39.0	15.0	40.0
5	32.803257065948856	38.0	32.0	39.0	12.0	40.0
6	35.081318977119786	38.0	35.0	39.0	25.0	40.0
7	35.71870794078062	38.0	35.0	39.0	29.0	40.0
8	35.51814266487214	38.0	35.0	39.0	27.0	40.0
9	35.592597577388965	38.0	35.0	39.0	27.0	40.0
10	34.68519515477793	38.0	35.0	39.0	24.0	40.0
11	34.782341857335126	38.0	34.0	39.0	26.0	40.0
12	33.85566621803499	38.0	32.0	39.0	22.0	40.0
13	33.50301480484522	38.0	32.0	39.0	20.0	40.0
14	34.09585464333782	38.0	33.0	39.0	23.0	40.0
15	33.08974427994617	38.0	32.0	39.0	18.0	40.0
16	32.51623149394347	37.0	30.0	39.0	19.0	40.0
17	31.554643337819652	36.0	28.0	38.0	15.0	40.0
18	30.907617765814265	35.0	26.0	38.0	13.0	40.0
19	32.07822341857335	36.0	29.0	38.0	17.0	40.0
20	32.88546433378197	37.0	30.0	39.0	21.0	40.0
21	33.05407806191117	38.0	31.0	39.0	19.0	40.0
22	32.27006729475101	37.0	29.0	39.0	16.0	40.0
23	32.97970390309556	38.0	31.0	39.0	19.0	40.0
24	32.376608344549126	37.0	29.0	39.0	17.0	40.0
25	32.86885598923284	37.0	30.0	39.0	19.0	40.0
26	33.31028263795424	38.0	32.0	39.0	19.0	40.0
27	33.62896366083446	38.0	33.0	39.0	22.0	40.0
28	33.58605652759085	38.0	33.0	39.0	22.0	40.0
29	33.0942934051144	38.0	32.0	39.0	19.0	40.0
30	32.90519515477793	38.0	31.0	39.0	18.0	40.0
31	33.726325706594885	38.0	33.0	39.0	22.0	40.0
32	33.645033647375506	38.0	33.0	39.0	21.0	40.0
33	33.44839838492597	38.0	33.0	39.0	20.0	40.0
34	33.31012113055181	38.0	33.0	39.0	19.0	40.0
35	33.17693135935397	38.0	33.0	39.0	19.0	40.0
36	32.656769851951545	37.0	32.0	39.0	17.0	40.0
37	32.359838492597575	36.0	31.0	38.0	15.0	40.0
38	31.801345895020187	36.0	30.0	38.0	10.0	40.0
39	30.584952893674295	35.0	28.0	38.0	4.0	40.0
40	28.511601615074024	34.0	22.0	38.0	4.0	39.0
41	26.88842530282638	32.0	18.0	38.0	4.0	39.0
42	27.69515477792732	33.0	22.0	38.0	4.0	39.0
43	27.734158815612382	33.0	22.0	38.0	4.0	39.0
44	26.7485868102288	32.0	18.0	38.0	4.0	39.0
45	27.71690444145357	33.0	23.0	38.0	4.0	39.0
46	25.759515477792732	30.0	17.0	36.0	4.0	39.0
47	24.70807537012113	29.0	11.0	36.0	4.0	39.0
48	25.42670255720054	30.0	16.0	36.0	4.0	39.0
49	24.340026917900403	29.0	10.0	36.0	4.0	39.0
50	25.06048452220727	30.0	14.0	36.0	4.0	39.0
51	25.394697173620457	31.0	10.0	36.0	4.0	39.0
52	25.197631224764468	31.0	4.0	36.0	4.0	39.0
53	25.034885598923285	30.0	4.0	36.0	4.0	39.0
54	24.64527590847914	30.0	4.0	36.0	4.0	39.0
55	24.318034993270526	30.0	4.0	36.0	4.0	39.0
56	23.345598923283983	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.55953714179428
1	2	4.290523298839425
1	3	6.551400575021333
1	4	7.894174639849954
1	5	8.2580418697071
1	6	4.789528175614386
1	7	4.306586549684752
1	8	4.58489691675139
1	9	4.566366106244281
1	10	5.025878618676067
1	11	-1.296946309723964
1	12	-7.486791730764683
1	13	-17.908767753937333
1	14	-6.052894642278037
1	15	-0.9823772971276448
1	16	-1.5952378414598272
1	17	-4.743745927937926
1	18	-4.142525449751481
1	19	-0.47502231590921085
1	20	-0.8716050440997094
1	21	7.258378786464323
1	22	7.863352530014986
1	23	7.152923656285402
1	24	7.506296037239057
1	25	6.465030993765062
1	26	6.598806124729023
1	27	5.968842631610183
1	28	6.254248755832155
1	29	6.31515621227323
1	30	7.07937666102109
1	31	4.545175107952026
1	32	3.836718935606445
1	33	4.502284955349747
1	34	3.879949160685065
1	35	5.096658749004266
1	36	3.738234095472478
1	37	3.741618764932273
1	38	4.599095924114913
1	39	5.208125083968497
1	40	-10.917756907019907
1	41	-1.2853343085993778
1	42	-2.339663678721312
1	43	-9.920552513414142
1	44	-15.41799312988012
1	45	-3.466216145236448
1	46	-5.675402550274498
1	47	-9.177425567537666
1	48	-4.482362170807203
1	49	-12.875787691362127
1	50	-4.158535025892281
1	51	0.7751708322468396
1	52	1.4132395997506002
1	53	1.4043242699462617
1	54	1.7874483867220405
1	55	0.5911898013930106
1	56	4.598886306768851
2	1	3.5458919176227397
2	2	4.460113942114276
2	3	6.670308957087613
2	4	7.8922253221111625
2	5	8.064084754697355
2	6	4.955220183411662
2	7	4.312434502901127
2	8	4.622908612657817
2	9	4.863637061409975
2	10	5.62139518787685
2	11	2.9213772770206745
2	12	0.5950796142645558
2	13	2.6029281524954158
2	14	4.648859743686877
2	15	5.782729915347989
2	16	5.885268981152262
2	17	7.4258447153369325
2	18	5.276577864088676
2	19	6.332969886819839
2	20	5.618648367206333
2	21	6.789567870284987
2	22	7.537816467636816
2	23	7.278654650437446
2	24	7.680759974860887
2	25	6.677506627293326
2	26	4.946759341103295
2	27	5.594573625762234
2	28	5.987192225617729
2	29	5.728411572897016
2	30	5.386394204880734
2	31	4.851217992942281
2	32	5.01703082644465
2	33	4.44672939979419
2	34	4.191839998891695
2	35	4.070342959530581
2	36	4.354218500930564
2	37	4.869299076823115
2	38	5.208257717487228
2	39	6.741263485527952
2	40	5.138773307405042
2	41	8.300435671907444
2	42	7.703221311532101
2	43	7.504398753642391
2	44	-3.35171632676121
2	45	7.143920307005267
2	46	5.650133512103668
2	47	2.7436270640412808
2	48	6.428943872077788
2	49	-0.5005440266447785
2	50	6.928209613483936
2	51	9.363864789361848
2	52	8.828444278113171
2	53	9.387755069166534
2	54	9.479456183992994
2	55	10.06389935304993
2	56	11.339627047509591
3	1	2.9148612158683562
3	2	3.918995521061646
3	3	6.138784834280592
3	4	7.5810959361462515
3	5	8.072856684521916
3	6	4.619967990429203
3	7	3.782280994129195
3	8	4.114410805640276
3	9	4.068680921059098
3	10	4.069860100157548
3	11	3.0022435050908527
3	12	3.0739721581242065
3	13	3.9697044682848883
3	14	3.530438691055295
3	15	-1.4140902600906067
3	16	5.456266788169803
3	17	6.827434627617631
3	18	7.627729179878148
3	19	5.678912869275976
3	20	5.3755014373817716
3	21	6.272846379056919
3	22	6.6729590114964665
3	23	6.526187545174288
3	24	7.138819185387202
3	25	5.7833180308021
3	26	4.782833902506802
3	27	5.02439818716574
3	28	5.029133015091414
3	29	4.807633064125085
3	30	5.1210433276877545
3	31	3.0891565894335073
3	32	3.684519861532369
3	33	3.27485439979419
3	34	3.0975417532776603
3	35	4.065408749004266
3	36	4.379437799176181
3	37	4.802961357524868
3	38	5.9127533315223175
3	39	6.5512963802647945
3	40	8.494858833720834
3	41	8.875545321030252
3	42	8.876192802760169
3	43	9.325396560659932
3	44	9.24751612937914
3	45	9.220126447356144
3	46	9.910824301577353
3	47	11.270491099129
3	48	9.092869310674278
3	49	10.972591938267502
3	50	10.6823214555892
3	51	9.39264768409869
3	52	9.182336821972822
3	53	10.209301121798113
3	54	9.409554868203521
3	55	10.416421282874492
3	56	8.844835380842923
4	1	3.9400628287715804
4	2	4.3837132629971265
4	3	6.778401769764464
4	4	8.3517612587269
4	5	8.347050232909012
4	6	5.217246216235651
4	7	4.525224542516291
4	8	4.812495483059628
4	9	4.687632533962322
4	10	5.862198809834965
4	11	4.904965279284404
4	12	5.950484254898399
4	13	5.958615758607472
4	14	5.828825787829487
4	15	6.022401675393265
4	16	7.405359530105283
4	17	9.069874143746663
4	18	9.406459018587828
4	19	7.8230660950824245
4	20	6.787295792220483
4	21	7.415487508089175
4	22	8.457737237302915
4	23	7.993425448400096
4	24	7.89637966925817
4	25	7.405293837253716
4	26	6.731422612184218
4	27	6.185688509746388
4	28	6.28770156347851
4	29	6.860556451221861
4	30	7.036365908332915
4	31	5.720204976530283
4	32	5.859419055080753
4	33	6.382213270761934
4	34	5.037057882309917
4	35	5.6450458457784585
4	36	6.974195863692309
4	37	6.640662970428092
4	38	6.683922686361029
4	39	8.246356864135763
4	40	2.3552418982369616
4	41	10.521714675868964
4	42	10.899882318889201
4	43	10.701908657434124
4	44	12.179975806798492
4	45	10.239279673162592
4	46	13.176449301577353
4	47	14.69891851848384
4	48	13.418474149383957
4	49	14.157067744719118
4	50	13.581010971718232
4	51	12.514119458292242
4	52	12.490300531650238
4	53	13.772305154056177
4	54	13.236671803687393
4	55	13.540413218358363
4	56	12.172960380842923
5	1	3.342702124959267
5	2	4.312745521061643
5	3	6.337932561553316
5	4	7.881380027055339
5	5	8.481947593612823
5	6	4.7861611722473825
5	7	4.060121903220107
5	8	3.8646948965493664
5	9	4.353908193786374
5	10	4.861905554703
5	11	3.826959414181765
5	12	3.802097158124205
5	13	4.9407271955576135
5	14	4.825893236509842
5	15	5.172841558091211
5	16	5.916209969987985
5	17	6.7209005367085375
5	18	8.014376907150872
5	19	6.464708323821434
5	20	5.582603710109048
5	21	5.76915319723874
5	22	6.581197647860101
5	23	5.905448908810655
5	24	5.6271714581144785
5	25	6.138431667165733
5	26	4.858402084324986
5	27	4.978943641711194
5	28	4.331689833273234
5	29	5.657917155034177
5	30	5.118770600415029
5	31	3.9721111348880527
5	32	3.4808266797141876
5	33	3.955820308885098
5	34	3.999814480550384
5	35	2.9057496580951785
5	36	4.023755980994359
5	37	4.14330226661578
5	38	4.524685149704133
5	39	5.568057743901157
5	40	6.532074742811744
5	41	9.17156804830298
5	42	9.323635984578349
5	43	8.862612469750843
5	44	9.604902493015505
5	45	9.217285538265237
5	46	9.994631119759173
5	47	10.461968371856276
5	48	9.771846583401551
5	49	10.726569210994779
5	50	10.71385554649829
5	51	9.790659047735055
5	52	8.890007276518276
5	53	8.792539758161748
5	54	8.905293504567158
5	55	10.009034919238129
5	56	9.072960380842922
6	1	3.387997180780637
6	2	4.144324468430064
6	3	6.161537027263051
6	4	7.699242865970813
6	5	7.993909316100861
6	6	5.253465797446744
6	7	3.680855555532702
6	8	4.324662998622735
6	9	4.425040570181906
6	10	5.042447819455795
6	11	4.377517627897873
6	12	5.261746280931227
6	13	4.9362614858287515
6	14	4.245350971757048
6	15	5.712554476751496
6	16	6.7624619636083985
6	17	8.215318399547453
6	18	8.030963829000957
6	19	7.490864623661942
6	20	6.1625080163291415
6	21	6.701848572039378
6	22	6.555360327285939
6	23	6.401461667981309
6	24	7.522865238018785
6	25	6.028383820275785
6	26	5.7362330253138225
6	27	5.699836783656966
6	28	4.759122050179133
6	29	5.552972976405783
6	30	4.561832801371967
6	31	2.7985864139949115
6	32	3.9994869667955264
6	33	4.043220627864368
6	34	3.981313683102222
6	35	3.807185064793739
6	36	5.6173763956674065
6	37	5.571053462788026
6	38	4.594222629767934
6	39	6.47810559079111
6	40	4.208948746001536
6	41	10.195172514012711
6	42	9.440063416795258
6	43	8.486854893993268
6	44	10.84126612937914
6	45	9.810586973671931
6	46	11.86065982789314
6	47	11.392749871058822
6	48	10.779821065060244
6	49	11.955596324232413
6	50	11.261542946817269
6	51	9.67965426304606
6	52	9.460023225481592
6	53	9.54564980600864
6	54	9.093491271712292
6	55	9.204250230242913
6	56	10.760679679088536
7	1	3.4736112158683596
7	2	4.552745521061645
7	3	6.530659834280591
7	4	7.990470936146249
7	5	8.41285668452192
7	6	5.024342990429204
7	7	4.171030994129197
7	8	4.533785805640278
7	9	4.7811809210590965
7	10	4.867360100157548
7	11	3.63786850509085
7	12	4.9020971581242065
7	13	5.73345446828489
7	14	4.460438691055295
7	15	6.494659739909391
7	16	6.5143917881697995
7	17	7.271809627617632
7	18	8.427104179878146
7	19	6.210162869275976
7	20	5.969876437381771
7	21	6.350971379056919
7	22	6.784834011496464
7	23	6.321812545174289
7	24	6.614444185387203
7	25	5.942068030802098
7	26	5.396583902506805
7	27	5.5643981871657395
7	28	5.8335080150914145
7	29	5.667008064125085
7	30	5.646043327687753
7	31	4.606656589433506
7	32	4.482644861532368
7	33	4.466729399794193
7	34	5.132541753277657
7	35	3.976658749004269
7	36	5.741937799176178
7	37	5.214211357524871
7	38	6.20650333152232
7	39	7.284421380264796
7	40	7.0629838337208355
7	41	10.724295321030255
7	42	11.361817802760168
7	43	10.253521560659934
7	44	10.48126612937914
7	45	10.42637644735614
7	46	12.096449301577355
7	47	11.989241099129
7	48	11.679119310674277
7	49	12.753841938267502
7	50	10.842946455589203
7	51	10.207022684098689
7	52	10.295461821972818
7	53	9.936176121798113
7	54	10.688929868203523
7	55	10.97267128287449
7	56	10.052960380842922
8	1	2.809049812359582
8	2	3.5302893807107694
8	3	5.459782641298133
8	4	6.997488480005902
8	5	7.257067210837704
8	6	4.271009657095867
8	7	2.803662573076565
8	8	2.9737858056402757
8	9	3.407496710532783
8	10	4.340693433490884
8	11	3.5705000840382226
8	12	4.016132245843508
8	13	4.848542187583135
8	14	3.999736936669329
8	15	5.659922897804133
8	16	6.622111086415419
8	17	6.846897346915878
8	18	7.574823478123765
8	19	7.104899711381236
8	20	6.530929068960717
8	21	6.543953835197268
8	22	7.222026993952603
8	23	5.401461667981309
8	24	7.329882781878428
8	25	6.010839960626662
8	26	4.701145306015576
8	27	4.489310467867494
8	28	3.864385208073873
8	29	4.728411572897016
8	30	4.4214819241789804
8	31	3.8512179929422814
8	32	3.859136089602547
8	33	3.4642732594433134
8	34	2.9462259638039754
8	35	4.263325415670931
8	36	4.073516746544598
8	37	4.711404339981005
8	38	4.945099822750386
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115	26	4.7144217403446405
115	27	4.632506295273846
115	28	5.56756206914547
115	29	3.6805215776386007
115	30	5.164962246606674
115	31	4.729359292136209
115	32	3.559942158829667
115	33	3.257540210605004
115	34	5.256325537061443
115	35	3.8939560463015646
115	36	4.252748609986995
115	37	5.050427573741086
115	38	6.317314142333132
115	39	6.085502461345875
115	40	7.904064914801914
115	41	7.087538564273494
115	42	6.466142127084492
115	43	5.6670350741734445
115	44	0.35477964289265174
115	45	3.639889960869656
115	46	0.28455740968546195
115	47	-9.145894036006135
115	48	-0.1495293379743714
115	49	-3.8812931968676345
115	50	1.2283518609946071
115	51	1.6270226840986908
115	52	1.3381645246755234
115	53	1.1075274731494638
115	54	1.9175785168521706
115	55	2.41050912071233
115	56	3.9702576781402215
116	1	3.2245203067774497
116	2	4.308200066516193
116	3	5.765205288826046
116	4	6.758652754328072
116	5	7.254674866340096
116	6	4.558888444974656
116	7	3.546485539583742
116	8	4.087422169276643
116	9	4.267544557422731
116	10	4.007360100157548
116	11	3.6996866869090326
116	12	3.2884607944878397
116	13	4.381636286466708
116	14	3.3713477819643884
116	15	-2.0817038964542434
116	16	4.738937242715256
116	17	6.493627809435811
116	18	6.191649634423602
116	19	5.028344687457796
116	20	4.768967346472678
116	21	5.441880469966012
116	22	6.8448340114964665
116	23	5.609994363356108
116	24	6.836262367205382
116	25	5.706613485347553
116	26	5.285674811597708
116	27	4.138034550802107
116	28	4.127144378727781
116	29	4.712462609579632
116	30	4.973316054960481
116	31	4.503929316706234
116	32	3.3535539524414624
116	33	4.060365763430557
116	34	4.72254175327766
116	35	3.4148405671860864
116	36	4.578301435539814
116	37	5.356938630252142
116	38	5.120139695158684
116	39	6.572603198446615
116	40	8.30025656099356
116	41	7.321568048302979
116	42	7.028181439123806
116	43	3.789885197023569
116	44	1.4321752202882294
116	45	4.740012810992507
116	46	-1.0735506984226468
116	47	-7.078940719052818
116	48	-0.6599715984166323
116	49	-2.6552489708234077
116	50	-0.40432627168352653
116	51	4.490659047735054
116	52	3.158189094700095
116	53	3.619812485434476
116	54	4.087111686385338
116	55	2.9817621919654016
116	56	5.945687653570197
117	1	2.5123990946562316
117	2	3.2400182483343727
117	3	5.113690137310897
117	4	6.1904709361462515
117	5	6.512250623915854
117	6	4.149797535883749
117	7	3.4783037214019217
117	8	3.822270654125127
117	9	3.487241527119707
117	10	4.098269191066642
117	11	-0.9063739191515694
117	12	-12.052448296421247
117	13	-10.186545531715112
117	14	-2.689258278641674
117	15	-4.384734199484544
117	16	-7.283790030012014
117	17	1.023930839738842
117	18	-2.5128958201218516
117	19	0.6647083238214293
117	20	0.47351280101813487
117	21	5.1085471366326765
117	22	6.647864314526771
117	23	5.844842848204593
117	24	5.1847472156902334
117	25	5.350552879286951
117	26	4.240220266143169
117	27	4.888034550802107
117	28	4.937750439333836
117	29	5.318523215640234
117	30	4.700588782233208
117	31	4.390292953069874
117	32	4.595978194865705
117	33	4.780062733127526
117	34	4.260420541156449
117	35	4.86938602173154
117	36	6.441937799176181
117	37	6.016029539343048
117	38	7.173169998188982
117	39	8.549875925719341
117	40	9.55025656099356
117	41	10.086719563454494
117	42	8.566060227002591
117	43	-4.08890268176431
117	44	1.9624782505912606
117	45	9.45970978068948
117	46	-0.5205203953923423
117	47	-0.1925770826891835
117	48	2.4991193106742777
117	49	0.7689934534190179
117	50	3.5199161525588956
117	51	7.930052987128995
117	52	8.908189094700095
117	53	8.210721576343566
117	54	9.450748050021705
117	55	7.6635803737835815
117	56	10.915384623267165
118	1	2.518226600483743
118	2	1.7819762902924126
118	3	4.079890603511359
118	4	5.498163243838558
118	5	6.30362591529115
118	6	3.181266067352283
118	7	2.4433386864368885
118	8	2.4737858056402757
118	9	2.496565536443711
118	10	3.468898561696008
118	11	-1.405208417986067
118	12	-7.67482591879887
118	13	-6.955776300945882
118	14	-12.411869001252398
118	15	-12.676109490859837
118	16	-10.465608211830197
118	17	-0.30357498776698577
118	18	-4.859049666275698
118	19	-0.6359909768778707
118	20	-0.8516620241566883
118	21	5.197125225210765
118	22	6.114064780727233
118	23	5.080274083635828
118	24	5.7698288007718155
118	25	4.886683415417487
118	26	4.838122364045262
118	27	4.216705879473434
118	28	4.551969553552954
118	29	4.859315756432778
118	30	5.284504866149295
118	31	3.372810435587354
118	32	4.608798707686216
118	33	4.677498630563424
118	34	4.97254175327766
118	35	4.558197210542726
118	36	5.3650147222531
118	37	5.372672895986408
118	38	6.121887946906931
118	39	7.581344457187875
118	40	10.0922146029516
118	41	9.844295321030252
118	42	8.834125495067862
118	43	5.5381369452753155
118	44	2.9566507447637562
118	45	5.93406875504845
118	46	1.3302954554235065
118	47	0.10462571451361669
118	48	0.15296546452043103
118	49	-1.0153888309632677
118	50	1.6306387632815067
118	51	6.280868837944844
118	52	5.943154129665128
118	53	4.3330991987211895
118	54	6.153545252818905
118	55	6.264978975182185
118	56	8.903729611612153
119	1	2.1336112158683562
119	2	3.0960788543949818
119	3	4.1162153898361495
119	4	5.801582047257366
119	5	6.600634462299695
119	6	3.7987874348736455
119	7	3.1121421052403093
119	8	3.4182302500847186
119	9	3.1589586988368765
119	10	2.2295823223797697
119	11	-0.5099092726869259
119	12	-8.570125064098015
119	13	-4.6309899761595545
119	14	-7.358450197833594
119	15	-9.750895815646164
119	16	-7.993385989607976
119	17	0.4229207387287417
119	18	-5.068451375677409
119	19	-0.03983713072402395
119	20	-1.0290124515071177
119	21	4.212082490168033
119	22	5.3448340114964665
119	23	4.152923656285402
119	24	3.9044441853872023
119	25	3.6409569196909857
119	26	4.793250569173466
119	27	4.635509298276855
119	28	4.319063570646971
119	29	4.805896953013971
119	30	5.607154438798869
119	31	2.998878811655729
119	32	3.929311528199033
119	33	3.585618288683076
119	34	2.916986197722103
119	35	4.652214304559823
119	36	3.2474933547317377
119	37	3.4453224686359825
119	38	5.20094777596676
119	39	4.58775471359813
119	40	7.378539389276391
119	41	4.677628654363584
119	42	3.75292891387128
119	43	-3.4298117726734
119	44	-1.0476227595097498
119	45	2.4874875584672544
119	46	-4.545772920644868
119	47	-4.899647789759889
119	48	-7.556436244881278
119	49	-5.246158061732498
119	50	-2.7881646555219106
119	51	1.7936893507653586
119	52	1.4965729330839324
119	53	0.7561761217981129
119	54	0.4078187570924108
119	55	1.294893505096713
119	56	3.284071491954034
120	1	2.1764683587255007
120	2	3.1698883782045044
120	3	5.524945548566308
120	4	7.047613793289106
120	5	7.115713827379061
120	6	4.204342990429204
120	7	3.4653167084149104
120	8	3.3452143770688494
120	9	3.1383237782019577
120	10	3.9787886715861163
120	11	-2.1964172091948626
120	12	-7.1121885561615095
120	13	-5.043688388857969
120	14	-9.233847023230421
120	15	-2.2453402600906074
120	16	-9.60846535468734
120	17	-1.673904658096653
120	18	-8.027181534407568
120	19	-2.67555141643831
120	20	-2.2044092769039416
120	21	2.5509713790569215
120	22	3.859119725782179
120	23	3.741812545174291
120	24	3.1830156139586343
120	25	3.473496602230668
120	26	4.819441045363945
120	27	4.3815410443085945
120	28	4.827793729377131
120	29	5.367008064125088
120	30	5.931757613402038
120	31	4.8266565894335045
120	32	4.262644861532369
120	33	3.9610151140799026
120	34	4.901113181849091
120	35	5.4252301775756955
120	36	6.127652084890464
120	37	6.691354214667722
120	38	6.620789045808031
120	39	7.032992808836227
120	40	7.994412405149404
120	41	-7.6985618218268925
120	42	-2.9438964829541163
120	43	-8.503621296482926
120	44	-16.273019584906574
120	45	-4.7021949812152855
120	46	-7.909264984136932
120	47	-7.925044615156715
120	48	-4.35802354646858
120	49	-14.831872347446783
120	50	-3.4627678301250846
120	51	1.1413083983844068
120	52	0.5497475362585362
120	53	1.6133189789409705
120	54	0.4689298682035208
120	55	1.8012427114459193
120	56	4.930103237985779
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1098.0
5	21.0
6	23.0
7	41.0
8	96.0
9	191.0
10	93.0
11	94.0
12	93.0
13	88.0
14	90.0
15	129.0
16	140.0
17	191.0
18	263.0
19	313.0
20	430.0
21	599.0
22	767.0
23	857.0
24	955.0
25	1136.0
26	1181.0
27	1265.0
28	1308.0
29	1320.0
30	1473.0
31	1713.0
32	1940.0
33	2070.0
34	2331.0
35	2430.0
36	2860.0
37	3217.0
38	3285.0
39	3048.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.41318977119785	0.3768506056527591	0.15343203230148048	0.05652759084791387
8	99.45087483176313	0.45222072678331093	0.05652759084791387	0.04037685060565276
9	98.3606998654105	0.8506056527590847	0.41184387617765816	0.3768506056527591
10	54.48452220726784	29.165545087483174	9.141318977119784	7.2086137281292055
11	36.00807537012113	23.911170928667563	21.63122476446837	18.449528936742933
12	30.76985195154778	19.098250336473754	23.030955585464334	27.100942126514134
13	25.111709286675637	16.75370121130552	24.99327052489906	33.14131897711978
14	23.33512786002692	23.380888290713326	28.164199192462984	25.119784656796767
15	29.630932409762433	19.72509334920721	25.031116402402727	25.612857838627633
16	30.501899296856056	23.683288881704787	23.667124653142597	22.14768716829656
17	28.106492947130395	21.753526434801334	28.580273500592224	21.559707117476044
18	26.62180349932705	21.208613728129205	24.75100942126514	27.418573351278603
19	26.41189302738951	25.6874309875845	21.8254288869139	26.075247098112094
20	27.091193273687615	24.514929395278646	27.347202759512772	21.046674571520967
21	26.021534320323013	22.347240915208612	27.329744279946166	24.301480484522205
22	24.277254374158815	21.582772543741587	29.528936742934054	24.611036339165544
23	24.048452220726784	23.327052489905785	27.895020188425303	24.72947510094213
24	24.56390653601809	23.473672876063315	26.090233659954777	25.87218692796382
25	25.215377988369585	24.83846650872281	24.332328236054277	25.613827266853328
26	27.014728452570075	25.595734941705484	22.70120358652629	24.688333019198147
27	23.41027229389426	25.920439548600825	22.413746667025773	28.255541490479143
28	24.422611036339166	27.991924629878866	22.909825033647376	24.675639300134588
29	29.49613134553689	26.123527350173887	21.46766236217076	22.912678942118458
30	26.258818460875656	23.78157143626474	27.49205665356239	22.467553449297213
31	28.972591675192504	26.054062786064293	22.777448710354857	22.19589682838835
32	31.43680327427433	24.06699337605687	23.55000269265981	20.946200657008994
33	29.90893415238711	23.361892445306605	23.65017782088587	23.07899558142041
34	24.737069197993637	25.10652068388976	28.94935548244431	21.207054635672293
35	23.52782643848538	27.139199568791266	29.40574046624444	19.92723352647891
36	25.591229865862196	28.15547055971556	24.2848677476701	21.96843182675214
37	27.806521153327946	24.87200215575317	25.89329021827001	21.428186472648882
38	27.079793975676186	27.012377639349566	24.18358816708465	21.724240217889598
39	27.556465958708426	26.006684275780284	22.330332596625517	24.106517168885773
40	22.444154573618132	19.32713734442089	23.191477797706398	35.037230284254576
41	17.67797810338254	18.98796230731521	21.114984476278664	42.219075113023585
42	23.323528618074857	25.81428609979221	24.53247699489975	26.329708287233185
43	21.701806009232513	20.516696811813297	26.81748238533596	30.964014793618226
44	22.170244984046292	24.08739386728679	24.633605537829215	29.108755610837704
45	25.6162684071417	23.82814607044609	26.487404930147257	24.068180592264955
46	22.863005561855392	26.14612020087478	24.59096063502349	26.399913602246343
47	19.5357519681844	23.274625977328682	25.344263182101017	31.845358872385898
48	24.32927486262256	23.822863915526344	28.259347053119278	23.588514168731816
49	22.953386056303813	22.88860697994548	24.96153742341224	29.19646954033847
50	23.4645456996495	23.704502561337286	27.422485845241308	25.408465893771908
51	21.834674153663407	25.867933696884617	27.66589277036877	24.631499379083202
52	21.33193186718413	26.05648986632169	28.085920655454938	24.525657611039243
53	21.647954226492498	25.48040591600993	29.37223361761848	23.49940623987909
54	22.736472810686816	24.477128592632575	28.48738361894481	24.299014977735798
55	21.26740796718126	24.085069631868723	26.962107308647305	27.685415092302712
56	22.526361209244627	24.651438742212996	28.83687063455678	23.9853294139856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	5.5
7	10.0
8	45.0
9	80.0
10	92.5
11	105.0
12	96.5
13	80.0
14	72.0
15	72.0
16	72.0
17	72.0
18	72.0
19	78.5
20	85.0
21	94.0
22	109.5
23	116.0
24	142.5
25	169.0
26	191.0
27	213.0
28	212.5
29	278.5
30	345.0
31	382.5
32	420.0
33	499.5
34	579.0
35	615.5
36	652.0
37	709.0
38	820.5
39	875.0
40	966.0
41	1057.0
42	1258.5
43	1460.0
44	1869.0
45	2278.0
46	2306.0
47	2180.5
48	2027.0
49	2063.0
50	2099.0
51	2179.0
52	2259.0
53	2324.0
54	2413.5
55	2438.0
56	2530.5
57	2623.0
58	2644.5
59	2666.0
60	2554.5
61	2443.0
62	2118.0
63	1575.0
64	1357.0
65	1172.5
66	988.0
67	796.0
68	604.0
69	540.0
70	476.0
71	416.0
72	273.0
73	190.0
74	156.0
75	122.0
76	96.0
77	70.0
78	67.0
79	40.5
80	17.0
81	10.5
82	4.0
83	2.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5168236877523553
16	0.08344549125168237
17	0.005383580080753701
18	0.0
19	0.05114401076716016
20	0.11305518169582773
21	0.0
22	0.0
23	0.0
24	0.005383580080753701
25	0.016150740242261104
26	0.029609690444145357
27	0.05652759084791387
28	0.0
29	0.15343203230148048
30	0.03230148048452221
31	0.021534320323014805
32	0.03230148048452221
33	0.09152086137281293
34	0.18304172274562586
35	0.12113055181695827
36	0.06460296096904442
37	0.10767160161507401
38	0.180349932705249
39	0.12920592193808883
40	0.9475100942126514
41	1.1628532974427996
42	0.2503364737550471
43	0.288021534320323
44	0.45222072678331093
45	0.19380888290713325
46	0.30148048452220727
47	0.5033647375504711
48	0.06998654104979811
49	0.2718707940780619
50	0.16150740242261105
51	0.2907133243606999
52	0.12382234185733512
53	0.26379542395693134
54	0.2557200538358008
55	0.26379542395693134
56	0.1857335127860027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	37150.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.74158815612383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65028355387523	71.4885598923284
2	6.781663516068052	10.815612382234185
3	1.7215770996489335	4.118438761776582
4	0.6447475020253848	2.056527590847914
5	0.30043208209559813	1.1978465679676986
6	0.1890359168241966	0.9044414535666219
7	0.13502565487442614	0.7537012113055181
8	0.12489873075884417	0.7967698519515478
9	0.060761544693491765	0.4360699865410498
>10	0.3645692681609506	5.004037685060565
>50	0.013502565487442613	0.6729475100942126
>100	0.013502565487442613	1.7550471063257065
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	225	0.6056527590847914	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	167	0.4495289367429341	No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	150	0.4037685060565276	No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	110	0.2960969044414536	No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	66	0.17765814266487212	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	64	0.17227456258411844	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	62	0.16689098250336473	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58	0.15612382234185734	No Hit
TCAAAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	49	0.13189771197846567	No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	48	0.12920592193808883	No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	40	0.10767160161507401	No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	38	0.10228802153432032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0026917900403768506	0.0	0.0	0.0	0.0
6	0.0026917900403768506	0.0	0.0	0.0	0.0
7	0.0026917900403768506	0.0	0.0	0.0	0.0
8	0.0026917900403768506	0.0	0.0	0.0	0.0
9	0.0026917900403768506	0.0	0.0	0.0	0.0
10	0.0026917900403768506	0.0	0.0	0.0	0.0
11	0.008075370121130552	0.0	0.0	0.0	0.0
12	0.010767160161507403	0.0	0.0	0.0	0.0
13	0.013458950201884253	0.0	0.0	0.0	0.0
14	0.018842530282637954	0.0	0.0	0.0	0.0
15	0.018842530282637954	0.0	0.0	0.0	0.0
16	0.024226110363391656	0.0	0.0	0.0	0.0
17	0.026917900403768506	0.0	0.0	0.0	0.0
18	0.026917900403768506	0.0	0.0	0.0	0.0
19	0.026917900403768506	0.0	0.0	0.0	0.0
20	0.029609690444145357	0.0	0.0	0.0	0.0
21	0.029609690444145357	0.0	0.0	0.0	0.0
22	0.029609690444145357	0.0	0.0	0.0	0.0
23	0.029609690444145357	0.0	0.0	0.0	0.0
24	0.03768506056527591	0.0	0.0	0.0	0.0
25	0.040376850605652756	0.0	0.0	0.0	0.0
26	0.04576043068640646	0.0	0.0	0.0	0.0
27	0.06729475100942127	0.0	0.0	0.0	0.0
28	0.07267833109017496	0.0	0.0	0.0	0.0
29	0.07267833109017496	0.0	0.0	0.0	0.0
30	0.08613728129205922	0.0	0.0	0.0	0.0
31	0.08613728129205922	0.0	0.0	0.0	0.0
32	0.08613728129205922	0.0	0.0	0.0	0.0
33	0.08613728129205922	0.0	0.0	0.0	0.0
34	0.08882907133243607	0.0	0.0	0.0	0.0
35	0.08882907133243607	0.0	0.0	0.0	0.0
36	0.09152086137281291	0.0	0.0	0.0	0.0
37	0.09152086137281291	0.0	0.0	0.0	0.0
38	0.09421265141318977	0.0	0.0	0.0	0.0
39	0.09421265141318977	0.0	0.0	0.0	0.0
40	0.09421265141318977	0.0	0.0	0.0	0.0
41	0.09421265141318977	0.0	0.0	0.0	0.0
42	0.09421265141318977	0.0	0.0	0.0	0.0
43	0.10228802153432032	0.0	0.0	0.0	0.0
44	0.10497981157469717	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCCGA	20	3.9509148E-4	50.45293	40
TAAGACC	15	0.008117193	50.384197	35
GTGCGCC	15	0.008117193	50.384197	35
CCTCGAC	15	0.008249388	50.179104	15
TAAGAAC	15	0.008249388	50.179104	34
CCCTCGA	15	0.008249388	50.179104	14
GATTTCC	20	4.0860582E-4	50.11111	9
ATCTGTG	15	0.008293807	50.11111	47
CACCATG	15	0.008338403	50.0433	49
GCACCAT	15	0.008338403	50.0433	48
CTCGACC	15	0.008473262	49.84097	16
TCGACCA	15	0.008473262	49.84097	17
TGCTGAT	15	0.008473262	49.84097	30
CTCAGAT	15	0.008473262	49.84097	16
CTCGTGT	15	0.008473262	49.84097	29
AGGGGTA	105	0.0	49.77389	5
AGGGGCG	35	5.29144E-8	49.77389	5
GGATGAA	20	4.2248494E-4	49.773888	8
GGATTTC	25	2.1040074E-5	49.773888	8
GCGGCCA	15	0.008518574	49.773888	22
>>END_MODULE