FastQCFastQC Report
Fri 17 Jun 2016
SRR1525969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT120.594059405940594No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC60.297029702970297No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.297029702970297No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.297029702970297No Hit
TCAAAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.24752475247524752No Hit
TCAAAGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC50.24752475247524752No Hit
TCAAAGGGGCGTGCACTTTTCTTCACTGAGACTGGGAAAG40.19801980198019803No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG40.19801980198019803No Hit
TCAAAGGGGATCAGAGTCCCTTTTACTTCTCTGTGAGTGA40.19801980198019803No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.19801980198019803No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.19801980198019803No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCAC30.1485148514851485No Hit
TCAAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.1485148514851485No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC30.1485148514851485No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG30.1485148514851485No Hit
TCAAAGGGGGGGGCTGGTCAGATGGCTCCGTGGGTAAGAG30.1485148514851485No Hit
TCAAAGGGGTACTTTTTGTTTTGTTTTGTTTTCTAGACAC30.1485148514851485No Hit
TCAAAGGGGATCAGAGTCACTTTTACTTCTCTGTGAGTGA30.1485148514851485No Hit
TCAAAGGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT30.1485148514851485No Hit
TCAAAGGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT30.1485148514851485No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCCGTGGTACAGCC30.1485148514851485No Hit
TCAAAGGGGCCATCTCATCCCTGCGTGTCTCCGACTCCGA30.1485148514851485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT200.002233637733.8255
AGGGGGG501.10958354E-1033.8255
AGGGGGC251.4381566E-433.8255
AGGGGAT200.002233637733.8255
AAGGGGG1050.033.8254
AAGGGGC200.002233637733.8254
AAGGGGA501.10958354E-1033.8254
TCAAAGG1950.033.8251
GGGGGGG309.06274E-633.8256
AAAGGGG1900.033.8253
CAAAGGG1900.033.8252
GGGGGGC250.00667114427.0600017
GGGGCTG250.00667114427.0600019
GGGCTGG250.00667114427.06000110