FastQCFastQC Report
Fri 17 Jun 2016
SRR1525968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGATTTTGTGGTAGTTTGCATTGTACCCCTTCT10.7142857142857143No Hit
TCAAAGGGGAATTGAATGGTGTTGCCTCCAGCGGCGCGAC10.7142857142857143No Hit
TCAAAGGGGACCAGTCCCTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGCCTTGTGCATGTAATCAACATTACCACCACA10.7142857142857143No Hit
TCAAAGGGGTACGCAGTCATTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGCAGCAGTGGTTCACCCCCACCACACACAACC10.7142857142857143No Hit
TCAAAGGGGACGCAGTGTTCACAGAGCTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACCCCTTATGACATGCCGCAGACCCTCAAGA10.7142857142857143No Hit
TCAAAGGGGTACCCATCGATTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACTTAAAAAAAAAAAACAAAACACCCCCCGC10.7142857142857143No Hit
TCAAAGGGGGGGGTTGGGCATTTAGCTCAGTGGTAGAGCC10.7142857142857143No Hit
TCAAAGGGGAGAGTGCAGTGCTTGAACGGGGAAAAAAAAC10.7142857142857143No Hit
TCAAAGCGGAAATAAAAATGTGTTGCCGTGTTGAGGCCTG10.7142857142857143No Hit
TCAAAGGGGACTACACGCAGAGCCCACTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAGTGCAGTGCTTCTACGGGAAAAAAAAAAAA10.7142857142857143No Hit
TCAAAGGGGAAGCAGTGGTATCCACCCCCCGGCCCCCCTC10.7142857142857143No Hit
TCAAAGGGGGCTTTTTTTCCGACCTCTTCGCGAGGCTGCT10.7142857142857143No Hit
TCAAAGGTAAATACAAAATTTTAACCAAGAGGCCTAGACT10.7142857142857143No Hit
TCAAAGGGGAATGACATCAATCCGCCCCCCCCCCCCTCCC10.7142857142857143No Hit
TCAAAGGGGATGAACGCAAAAACAATTTCAAACCTCTTCT10.7142857142857143No Hit
TCAAAGGGGGGGTTGAATTCATTCTTAGAGGAAATGAAGA10.7142857142857143No Hit
TCAAAGGGGACATGGTATCAACGCACCACACACCCCACCC10.7142857142857143No Hit
TCAAAGGGGAGGACCTAAAGCTAAGTCCAACATAAGTGGC10.7142857142857143No Hit
TCAAAGGGGAAAAGTGGTACAAAACCCCCCCCCCTCTTTT10.7142857142857143No Hit
TCAAAGGGGAGAGCCGACTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGAGGGGCTGGAGCGATGGCTTCGTGGTTACG10.7142857142857143No Hit
TCAAAGGGGAAGCCTGGTATCAACCNNCNCCCCCCACCCC10.7142857142857143No Hit
TCAAAGGGGAAGTATCAACGCGCGCCGCCTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGTTAGTTAACCCATTACACTATGTCTGTTTGGCC10.7142857142857143No Hit
TCAAAGGGGAAGCATCCTACAACCCCGAGCCCCCTTTTTT10.7142857142857143No Hit
TCAAAGGGGAAGTGGTATCACAGAGCCGACTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGAATCAGAATTAGTATCAGGGTTTAACGTCG10.7142857142857143No Hit
TCAAAGGGGATCGCCTTTTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGTCGCATGCATATATAAACTTTATACTTATA10.7142857142857143No Hit
TCAAAGGGGATGGTATCAACGCAAAAAAAACACAAAAAAA10.7142857142857143No Hit
TCAAAGGGGTACCCGGTTGATCCTGCCAGACGCCTATGCT10.7142857142857143No Hit
TCAAAGGGGTAAGACGTTTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGAGAAATCAAAAAAAAAAACACCCCCAACCACATC10.7142857142857143No Hit
TCAAAGGGGGAGAGAAAACAAAAAAAAAAAAAAAAAAAAC10.7142857142857143No Hit
TCAAAGGGGACTGTACAACCCCCAGCCGCCTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGCAGTTCAACGCAGAGCGATTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAAGCATTATCACCCCGAGCCGCCTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGATTGTTAATCCAAAAAAAAAAAAAAAAAAAC10.7142857142857143No Hit
TCAAAGGGGAAGCCTGTATCAACCCCGAGCCGCCTTTTTC10.7142857142857143No Hit
TCAAAGGGGACAAGAGGGGACTGTGGAACCCCCCGCCCCT10.7142857142857143No Hit
TCAAAGGGGCACTGGTCCAGAGCGATTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGATCCGAAAAAAAAAAAAAAAAAAAAAAAAAA10.7142857142857143No Hit
TCAAAGGGGAAACAAAGTCTTGCCCACTTCTAACCCTTTG10.7142857142857143No Hit
TCAAAGGGGAACTGCGTGCCTCAACAAGTTGACACACGAA10.7142857142857143No Hit
TCAAAGGGGCTCCAAAAAGAAAAACCCCCCCCACGCTCTA10.7142857142857143No Hit
TCAAAGGGGCATGGTACAACCAGAGCCGACTTTTTCTTTT10.7142857142857143No Hit
TCAAAGGTAAATAAAATACATGTACGTGAGCTTCTTAACT10.7142857142857143No Hit
TCAAAGGCGAACAAAACCCCCCCCCCCCCCCCTCCCCCCC10.7142857142857143No Hit
TCAAAGGGGGTGATTGGCTATCCCCTCTCTGCCAGAAGGC10.7142857142857143No Hit
TCAAAGGGGGGAGTGCAGTGCTGCAAACAAAAAAAAAAAA10.7142857142857143No Hit
TCAAAGCACCACAAAAAACACCCCCCACCCAAAGTTCTTC10.7142857142857143No Hit
TCAAAGCGGGAAAGGTCACGAAAAAAATAACAATAGAGAG10.7142857142857143No Hit
TCAAAGGGGACTGGTATACGCAGATCGACTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGCCTCTAATACAACCTCCCGGTGGCAATTCCTCTG10.7142857142857143No Hit
TCAAAGGGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC10.7142857142857143No Hit
TCAAAGGGGATTCCCATCCTGTCTCCCAGCCACGCCCACC10.7142857142857143No Hit
TCAAAGGGGTAAAAAATAAGGACAACCCATCACTAGTACA10.7142857142857143No Hit
TCAAAGGTAAACTCTAACCCGACCAACATTTATTTTTTTT10.7142857142857143No Hit
TCAAAGGGTAATATGATGCTCTTCGATCGTTGATTTGATT10.7142857142857143No Hit
TCAAAGGGGAATCCTTTGGATCAACCCACAACCCGCATCC10.7142857142857143No Hit
TCAAAGGGGGGGAGGCCCTGTTTTAAATTTTCCCTTTCTG10.7142857142857143No Hit
TCAAAGGGGCGCCACCCGAGCCTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGAAAAAATAACTCACCACCCACCAACTGAGGCT10.7142857142857143No Hit
TCAAAGGGGCCGTACTTCCCAGGGGTTCCGCCGCGTCGCC10.7142857142857143No Hit
TCAAAGGGGAAAGACAACAAACCCCCCACCCCCGGCACGA10.7142857142857143No Hit
TCAAAGGGGATAAAAGAAGTGATCCCCCCTCCACCCCCCC10.7142857142857143No Hit
TCAAAGGGGCAGTTACGCAGAGTCGCTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGTCGGAAGCACTCGTATCCCGTCTCCTCCTTC10.7142857142857143No Hit
TCAAAGGGACAGTGTATCAACGCAGAGCCGACTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGGCGGTCTCCTTCCTTTGCTCGTTCTTCTTC10.7142857142857143No Hit
TCAAAGGGGGTGGTCCTTTGGCCTAGCGCCCCGGCTTCCC10.7142857142857143No Hit
TCAAAGGGGAGCCAGAGTCGATTTTTTTTTCTTTTTTTTT10.7142857142857143No Hit
TCAAAGAGGGAGTGCAGTGCTTCAAAGGGGCAGTGGTATC10.7142857142857143No Hit
TCAAAGGGGCAGCAGTGTATCAACCCCCTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGTCTCACTAAAAAACCAAACACAACCCAACCA10.7142857142857143No Hit
TCAAAGGGGCAGAGTGCAATGCTTCAAAGGGGGATCCAGT10.7142857142857143No Hit
TCAAAGGGGAGTGCAATGCTTCAAAGGGGCTGGTCCCCCG10.7142857142857143No Hit
TCAAAGGGGGTGCTTCAAAGGGGACGTGTCCACGCGACTT10.7142857142857143No Hit
TCAAAGGGGATTTAAAAAAAAAAAAAAAAAAACCCCCCGG10.7142857142857143No Hit
TCAAAGGGGAGTCTCAGATTTGCTCCTTGTATGTCTAGGG10.7142857142857143No Hit
TCAAAGGGTATTCAACCAACACCCTCCGCTTCCACAGCAC10.7142857142857143No Hit
TCAAAGGGGACGCATAGTATAACCCCGAGCCGCCTTTTTT10.7142857142857143No Hit
TCAAAGGGGATGGTCAGAGTCGAAACCAAACACCAAAAAA10.7142857142857143No Hit
TCAAAGGGGCACTGTATAACCCCGCGTCGCCTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAGTGCTTCAAAGGGGACATTACACGCAGAGT10.7142857142857143No Hit
TCAAAGGGGGGGAATTACTAGAAAAACACATCCCTCCCCT10.7142857142857143No Hit
TCAAAGGGGGAGAAGAATCACTTTCCACCCCACTATCATC10.7142857142857143No Hit
TCAAAGGGGGGATGTGGATACCCCCCCCCCCCCCCCCCCC10.7142857142857143No Hit
TCAAAGGGAGTGCAGTGCTTCAAANGGCCCACCCCATCCC10.7142857142857143No Hit
TCAAAGGGGAGATGTTCGATGCTACGCCCCTGATGCCTGC10.7142857142857143No Hit
TCAAAGGGGAAGCAGTTACAACGCAGAGNCGACTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACTTAGTCAAAGATTTGGACTGGAAATGGGT10.7142857142857143No Hit
TCAAAGGGGCAGGACAACCCAGAGCCGCCTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGTTTTTGCTGACTCTGCCCCACTCCAGAGAC10.7142857142857143No Hit
TCAAAGGGGCCAGTGGTACCAACCCAAAAAAACACAAACA10.7142857142857143No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGGGAAAACAAAA10.7142857142857143No Hit
TCAAAGGGGTATCAACGCAGAGTCGACTTCTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGCACTGGTGCATTTAAAATGGCCTTCTGTTCA10.7142857142857143No Hit
TCAAAGGGGATATCGTTTTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACCCCTGCCTGTGTTTGTCAGTGATCCTTAT10.7142857142857143No Hit
TCAAAGGGGAGATGGTTCAACCAAACAAAACCCCCACCCA10.7142857142857143No Hit
TCAAAGGGGCAAAACGCACAGTCGACTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACAGTGCACCCCAGAGTCGCCTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGTGGCCAGCCGCCTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGAGATACTAACCAAATTCGCCTCATCCCCCCTA10.7142857142857143No Hit
TCAAAGGGGATGCCTACTAACCAGAGCAGACCCCAGGCCC10.7142857142857143No Hit
TCAAAGGGGAAGTTCACCGACTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGCTTAATGTTCACTTTGACTCTGTGTTTGGAC10.7142857142857143No Hit
TCAAAGGGACGTTAACCACCGCCGACCTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAGCCAGTTTTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGTATTCAGCTGTATAGCTTCCCCTTGGCTCTG10.7142857142857143No Hit
TCAAAGGGGAGTCCAGCCTTCCCCCCGTCTCTCTCCTTGT10.7142857142857143No Hit
TCAAAGGGGACTGGTCAACAGAGTCGACTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGTTTTATTCACTTAATCCAAAGAACCAACTAA10.7142857142857143No Hit
TCAAAGGGGATGCCGAGAGTCGACTTTTTTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACTCAACAACCAGACCCTCTCCTCTTTTTAT10.7142857142857143No Hit
TCAAAGGGGAAGAATAACAAACCACCCCCCCTAGACCACG10.7142857142857143No Hit
TCAAAGGGGCAGTGGTCCCCCCGCGTCGCCTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGACCAAAACAAAAAACAAAAAAACCAAAAAAA10.7142857142857143No Hit
TCAAAGGGGAAAAAAACATGATCAACCGCAGGGCGCAACA10.7142857142857143No Hit
TCAAAGGGGGTGGTTTTGCAGTGGGAACTGTAACTCAACC10.7142857142857143No Hit
TCAAAGGGGATGCCTTCCCCCCCCCCCCCCCCCCCCCGCG10.7142857142857143No Hit
TCAAAGGGGAGTTACCGGTCATCTTGGGTACATAGAGTTT10.7142857142857143No Hit
TCAAAGGGGGGTCTGACATTGCCGCCTTTCCTAAGGGTCA10.7142857142857143No Hit
TCAAAGCTCACTTTTTTCCTTTCACACATTCCCAAACTGC10.7142857142857143No Hit
TCAAAGGGGACTGGTTCAACGAAGAACCGACTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAAGCAGTAGCATAATACCCCGCCTCCAAACT10.7142857142857143No Hit
TCAAAGGGGGCCTGGGGACCCCCACCTATGACGCACCTGC10.7142857142857143No Hit
TCAAAGGGGAATAATTCCCCATAACCCCCAAACCACCCCC10.7142857142857143No Hit
TCAAAGGGGGTGCNTCAAAGGGGAAGTGCCCAAGCGACTT10.7142857142857143No Hit
TCAAAGGGGCAGTGTATCACGAGATCGACTTTTTTTTTTT10.7142857142857143No Hit
TCAAAGGGGAAGCGTGTATCCACCCCGCGTCGCCTTTTTT10.7142857142857143No Hit
TCAAAGGGGATGTCACCAGAGTCGCTTTTTTTTTTTTTTT10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAGG100.034.01
AAAGGGG100.034.03
CAAAGGG100.034.02
Produced by FastQC (version 0.11.5)