FastQCFastQC Report
Fri 17 Jun 2016
SRR1525967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT110.6145251396648044No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.5586592178770949No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.44692737430167595No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT70.3910614525139665No Hit
TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG60.33519553072625696No Hit
TCAAAGGGGATTGGGCGGATTGAACAAAAACTCATCTCAG60.33519553072625696No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.27932960893854747No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG50.27932960893854747No Hit
TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGC40.22346368715083798No Hit
TCAAAGGGATGGAAGATATATGTCTGATGTATCTTCTCAC40.22346368715083798No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.16759776536312848No Hit
TCAAAGGGGGGGAGTTGACTGTCTAAAAGCTACCAGAGCA30.16759776536312848No Hit
TCAAAGGGGGGTTTCGACGCTAGAGGTAACCTCAGCATTC30.16759776536312848No Hit
TCAAAGGGGGTGCGTTGCCGTGGCTGATGGGCGCTGTCTG30.16759776536312848No Hit
TCAAAGGGGATAATAATTCGTTTTATGCATCATTAAGTAG30.16759776536312848No Hit
TCAAAGGGGGATTCTTAAAGGGGCCCAAGAGATCGCACGG30.16759776536312848No Hit
TCAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.16759776536312848No Hit
TCAAAGGGGAGTCTTTTGCCTTGCCTTTATTACATGTAAA30.16759776536312848No Hit
TCAAAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT30.16759776536312848No Hit
TCAAAGGGGTGTTGATTTTTTTGATGATAGACTATATACA30.16759776536312848No Hit
TCAAAGGGGGACTTGGGTTCCGGCCTATGCAAGTCTGCAC30.16759776536312848No Hit
TCAAAGGGGGTTTTATCCAACTCATCCCTTGACATCGTGC30.16759776536312848No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC30.16759776536312848No Hit
TCAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT30.16759776536312848No Hit
TCAAAGGGGGGCCTGGTGAGATGGCTCAGTGGGTAAGAGC20.11173184357541899No Hit
TCAAAGGGGATTGTTTGGTTCCAGAAAACCAACCACCTTT20.11173184357541899No Hit
TCAAAGGGGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAA20.11173184357541899No Hit
TCAAAGGGGGTTTGGGTCAGATTCTAGCTTTTGTCACGTT20.11173184357541899No Hit
TCAAAGGGGCTTCGTAGGAGTAAACATAACATTCTTCCCT20.11173184357541899No Hit
TCAAAGGGGAGGGCAGGAGTTGGGGAAAGACGCGCAGAAA20.11173184357541899No Hit
TCAAAGAGGGAGCCTTAGGCTTTATTTTCTTATTTACAGT20.11173184357541899No Hit
TCAAAGGGGAACTTTATTCTTTTTTTTCTCCTGCTGAAAC20.11173184357541899No Hit
TCAAAGGGGGAAGTTTCATTACCTGATCCGTGGGGCTTTG20.11173184357541899No Hit
TCAAAGGGGATGTCTCTTCCTGTCTGTTTCTTTACCTCTC20.11173184357541899No Hit
TCAAAGGGGAGTATTTAGCTGAGGGCAGTTATGTTTCTCC20.11173184357541899No Hit
TCAAAGGGGGCCACTGCTCTTTCAAATTCAAAATGTTATC20.11173184357541899No Hit
TCAAAGGGGGATCCTGTGGATAAAATTGTCTTGCAAAAAT20.11173184357541899No Hit
TCAAAGGGGGTGCAGTGCTTCAAAGGGGTCACAGATCCTC20.11173184357541899No Hit
TCAAAGGGGATGAGTGGACCAGCGGGTCCAACACAGGGAA20.11173184357541899No Hit
TCAAAGGGGCGGAATTGTTTTATCCAACTCATCCCTTGAC20.11173184357541899No Hit
TCAAAGGGGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA20.11173184357541899No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC20.11173184357541899No Hit
TCAAAGGGGGGGTTCGTGTGTTGTTCGGGATGCCACTGGC20.11173184357541899No Hit
TCAAAGGGGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11173184357541899No Hit
TCAAAGGGGGAGAGGCTCAGAGGATTCTGACAGCTTCTTT20.11173184357541899No Hit
TCAAAGGGGACAATTTGCTGTGCCTTTATTCCAGAGTCGG20.11173184357541899No Hit
TCAAAGGGGAAGTTTAACGGCCGCGGTATCCTGACCGTGC20.11173184357541899No Hit
TCAAAGGGGTACAGTTGGTGCTCTAACCGGAATTGTTTTA20.11173184357541899No Hit
TCAAAGGGGATCAGTGGGTGGAGGGAAGGAAGAGAGGCTA20.11173184357541899No Hit
TCAAAGGGGGTTTCCCCACCAGCATGATTGGCGTGGGCCT20.11173184357541899No Hit
TCAAAGGGGATTAGAAAAATTATTTAGATACTTGACCATT20.11173184357541899No Hit
TCAAAGGGGATTGTTTTCCTGGTTTCACTGAGTTGCCGCA20.11173184357541899No Hit
TCAAAGGGGGATCTGGTGTACTCCACTCGTCTGGTGTTCG20.11173184357541899No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA20.11173184357541899No Hit
TCAAAGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAG20.11173184357541899No Hit
TCAAAGGGGGAGAACTTAAGCTGTGGAGACTGAGACCACC20.11173184357541899No Hit
TCAAAGGGGATTGCATACTATTTTAAAGATGTTTTAATGT20.11173184357541899No Hit
TCAAAGGGGGAGCTGGAGAGATGGCTCAGTGGGTAAGAGC20.11173184357541899No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.11173184357541899No Hit
TCAAAGGGGTGAGGTGTGGGTGTGTTCTATATTCCAAATT20.11173184357541899No Hit
TCAAAGGGGTGGCATAATACACACCAAAACCACCCAGATT20.11173184357541899No Hit
TCAAAGGGGGCTTTGGGACTCTGTTAACTTTGGGGAAGGA20.11173184357541899No Hit
TCAAAGGGGACCCAAGGGATGGTTTCAGGGACTGAGGTGT20.11173184357541899No Hit
TCAAAGGGGAACCCTGGGTTGATTAGCATATGCTTCTAAA20.11173184357541899No Hit
TCAAAGGGGAAGATTGCATTTTTATCTAAGGAATGATGTT20.11173184357541899No Hit
TCAAAGGGGGATCTGGAGAGATCGCTTAGTAATCAAATGC20.11173184357541899No Hit
TCAAAGGGGGCTTTTTAAAGAAAAATATATATATTGTTTC20.11173184357541899No Hit
TCAAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.11173184357541899No Hit
TCAAAGGGGAGTGCAGTGCTTCAAAGGGGTCACAGATCCT20.11173184357541899No Hit
TCAAAGGGGAGGATTAGATGTAGACACACGAGCTTACTTT20.11173184357541899No Hit
TCAAAGGGGATTGGTAAAAAAAAAAAAAAAAAAAAAAAAA20.11173184357541899No Hit
TCAAAGGGGGACGCTACTAAGACTACCCAGATGCTTACAC20.11173184357541899No Hit
TCAAAGGGGGGGGTTCGATTCCTTTATTTTCTTATTTACA20.11173184357541899No Hit
TCAAAGGGGGATCCAAAGGGTTTTGGAGGGTTTCTATAGG20.11173184357541899No Hit
TCAAAGGGGCATTCTGTATTGGAAATGCTGTGAAACTGTA20.11173184357541899No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG20.11173184357541899No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGC20.11173184357541899No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.11173184357541899No Hit
TCAAAGGGGATTTTATGTGTATGAGTGTTTTGCTTGCATG20.11173184357541899No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.11173184357541899No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAG20.11173184357541899No Hit
TCAAAGGGGGAGTGCAGTGCTTCAAAGGGGAGTCGAAGTG20.11173184357541899No Hit
TCAAAGGGGTCTTATGTGGAATCAGAAATTCTTATTAATG20.11173184357541899No Hit
TCAAAGGGGGTGCGTTGCCGTGGATGATGGGCGCTGTCTG20.11173184357541899No Hit
TCAAAGGGGAACAGGAACTTTTTTTTTTGAAGATTTGAAA20.11173184357541899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT200.002098324734.05
AGGGGGC251.318037E-434.05
AGGGGGA251.318037E-434.05
AAGGGGA308.064844E-634.04
TCAAAGG1700.033.01
AAAGGGG1700.033.03
CAAAGGG1700.033.02
AAGGGGG1150.032.5217364
AGGGGGG457.8429366E-830.2222215
GGGGGGG551.6455146E-521.6363626
Produced by FastQC (version 0.11.5)