##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525967_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1790 Sequences flagged as poor quality 0 Sequence length 40 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.701675977653633 29.0 4.0 31.0 4.0 33.0 2 12.858100558659219 4.0 4.0 22.0 4.0 29.0 3 12.494972067039106 4.0 4.0 21.0 4.0 28.0 4 11.949720670391061 4.0 4.0 20.0 4.0 29.0 5 11.040782122905028 4.0 4.0 17.0 4.0 28.0 6 10.820670391061453 4.0 4.0 18.0 4.0 26.0 7 13.515083798882682 4.0 4.0 26.0 4.0 31.0 8 13.034078212290503 4.0 4.0 23.0 4.0 31.0 9 13.082122905027934 4.0 4.0 24.0 4.0 31.0 10 12.184916201117318 4.0 4.0 21.0 4.0 30.0 11 13.280446927374301 4.0 4.0 27.0 4.0 33.0 12 13.076536312849163 4.0 4.0 25.0 4.0 33.0 13 12.318435754189943 4.0 4.0 23.0 4.0 32.0 14 12.95586592178771 4.0 4.0 26.0 4.0 33.0 15 12.621229050279329 4.0 4.0 25.0 4.0 33.0 16 10.420111731843575 4.0 4.0 17.0 4.0 30.0 17 9.678212290502794 4.0 4.0 13.0 4.0 27.0 18 9.401117318435753 4.0 4.0 12.0 4.0 27.0 19 9.264804469273743 4.0 4.0 11.0 4.0 27.0 20 9.748603351955307 4.0 4.0 13.0 4.0 29.0 21 8.383798882681564 4.0 4.0 5.0 4.0 24.0 22 8.341340782122906 4.0 4.0 4.0 4.0 24.0 23 8.339106145251396 4.0 4.0 4.0 4.0 24.0 24 8.045810055865921 4.0 4.0 4.0 4.0 23.0 25 8.332960893854748 4.0 4.0 4.0 4.0 25.0 26 8.611731843575418 4.0 4.0 4.0 4.0 26.0 27 8.610614525139665 4.0 4.0 4.0 4.0 27.0 28 9.016201117318436 4.0 4.0 4.0 4.0 28.0 29 8.115083798882681 4.0 4.0 4.0 4.0 24.0 30 8.436871508379888 4.0 4.0 4.0 4.0 25.0 31 9.099441340782123 4.0 4.0 4.0 4.0 30.0 32 9.040782122905028 4.0 4.0 4.0 4.0 30.0 33 8.872625698324022 4.0 4.0 4.0 4.0 29.0 34 8.868715083798882 4.0 4.0 4.0 4.0 29.0 35 8.997206703910615 4.0 4.0 4.0 4.0 30.0 36 8.883798882681564 4.0 4.0 4.0 4.0 30.0 37 8.809497206703911 4.0 4.0 4.0 4.0 30.0 38 8.748603351955307 4.0 4.0 4.0 4.0 31.0 39 8.424581005586592 4.0 4.0 4.0 4.0 30.0 40 8.480446927374302 4.0 4.0 4.0 4.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 10.393512767425811 1 2 -8.67427536231884 1 3 -7.434851621808146 1 4 -7.343788819875776 1 5 -7.415458937198068 1 6 -6.017460317460319 1 7 -9.028088336783991 1 8 -8.274499654934436 1 9 -8.217339544513457 1 10 -7.234834368530018 1 11 -7.049482401656315 1 12 -6.5467908902691505 1 13 -7.2150621118012435 1 14 -7.188854382332641 1 15 -6.822826086956521 1 16 -5.369254658385094 1 17 -4.85146652864044 1 18 -4.468219461697721 1 19 -4.177795031055902 1 20 -4.875465838509319 1 21 -3.769392684610075 1 22 -3.6792788129744665 1 23 -4.04696342305038 1 24 -3.851552795031056 1 25 -3.6020531400966194 1 26 -3.6218426501035195 1 27 -3.515510697032436 1 28 -3.664837819185646 1 29 -3.1790545203588687 1 30 -3.144565217391305 1 31 -3.9336956521739124 1 32 -3.838077984817115 1 33 -4.0992581090407185 1 34 -4.142598343685302 1 35 -3.975810904071773 1 36 -3.7889061421670123 1 37 -3.607436162870946 1 38 -3.9064009661835764 1 39 -3.2084368530020706 1 40 -3.9527950310558992 2 1 14.393512767425811 2 2 17.825724637681162 2 3 20.065148378191854 2 4 9.656211180124224 2 5 22.584541062801932 2 6 13.48253968253968 2 7 15.971911663216009 2 8 3.725500345065564 2 9 -6.717339544513457 2 10 -0.7348343685300183 2 11 -5.549482401656315 2 12 2.9532091097308495 2 13 5.7849378881987565 2 14 5.811145617667359 2 15 6.177173913043479 2 16 1.1307453416149063 2 17 5.14853347135956 2 18 1.0317805383022787 2 19 10.322204968944098 2 20 5.624534161490681 2 21 9.230607315389925 2 22 -3.1792788129744665 2 23 3.9530365769496196 2 24 1.648447204968944 2 25 1.3979468599033806 2 26 6.3781573498964805 2 27 8.984489302967564 2 28 5.835162180814354 2 29 6.820945479641131 2 30 3.855434782608695 2 31 11.566304347826087 2 32 11.661922015182885 2 33 7.4007418909592815 2 34 10.857401656314698 2 35 8.524189095928227 2 36 11.711093857832989 2 37 5.392563837129054 2 38 9.593599033816425 2 39 5.791563146997929 2 40 11.5472049689441 4 1 -9.106487232574189 4 2 -4.9242753623188396 4 3 -5.934851621808146 4 4 -6.593788819875776 4 5 -6.665458937198068 4 6 -4.517460317460319 4 7 -7.528088336783991 4 8 -3.274499654934436 4 9 -1.9673395445134574 4 10 -3.2348343685300183 4 11 -5.799482401656315 4 12 -5.2967908902691505 4 13 -2.4650621118012435 4 14 -0.6888543823326412 4 15 -5.322826086956521 4 16 -0.8692546583850937 4 17 1.3985334713595599 4 18 2.2817805383022787 4 19 3.5722049689440976 4 20 -0.375465838509319 4 21 2.230607315389925 4 22 -2.4292788129744665 4 23 -2.5469634230503804 4 24 -2.351552795031056 4 25 1.8979468599033806 4 26 0.37815734989648053 4 27 0.4844893029675639 4 28 3.585162180814354 4 29 0.07094547964113129 4 30 0.855434782608695 4 31 -0.6836956521739124 4 32 -1.5880779848171152 4 33 2.6507418909592815 4 34 -0.142598343685302 4 35 4.024189095928227 4 36 -0.5389061421670123 4 37 -0.35743616287094593 4 38 2.5935990338164236 4 39 4.791563146997929 4 40 0.047204968944100756 6 1 11.393512767425811 6 2 3.8257246376811604 6 3 8.565148378191854 6 4 15.156211180124224 6 5 12.584541062801932 6 6 -5.017460317460319 6 7 -3.0280883367839913 6 8 1.225500345065564 6 9 3.2826604554865426 6 10 -3.7348343685300183 6 11 -3.0494824016563147 6 12 4.9532091097308495 6 13 7.2849378881987565 6 14 5.811145617667359 6 15 7.677173913043479 6 16 7.630745341614906 6 17 9.64853347135956 6 18 3.5317805383022787 6 19 7.322204968944098 6 20 6.624534161490681 6 21 9.230607315389925 6 22 8.820721187025534 6 23 8.95303657694962 6 24 10.148447204968944 6 25 10.397946859903382 6 26 9.87815734989648 6 27 2.984489302967564 6 28 3.335162180814354 6 29 6.320945479641131 6 30 4.855434782608695 6 31 3.0663043478260876 6 32 3.161922015182885 6 33 10.400741890959281 6 34 2.857401656314698 6 35 3.024189095928227 6 36 1.7110938578329877 6 37 5.392563837129054 6 38 10.593599033816425 6 39 3.2915631469979294 6 40 9.5472049689441 7 1 -2.856487232574189 7 2 0.07572463768116044 7 3 -2.6848516218081464 7 4 0.9062111801242239 7 5 -6.165458937198068 7 6 -5.517460317460319 7 7 -7.528088336783991 7 8 -5.774499654934436 7 9 -3.4673395445134574 7 10 -3.7348343685300183 7 11 -2.5494824016563147 7 12 -1.5467908902691505 7 13 -0.9650621118012435 7 14 -5.688854382332641 7 15 -2.322826086956521 7 16 -2.6192546583850937 7 17 -0.8514665286404401 7 18 -2.9682194616977213 7 19 -1.9277950310559024 7 20 -0.625465838509319 7 21 0.48060731538992485 7 22 0.8207211870255335 7 23 -1.0469634230503804 7 24 -3.601552795031056 7 25 -2.1020531400966194 7 26 1.3781573498964805 7 27 2.984489302967564 7 28 1.5851621808143541 7 29 1.8209454796411313 7 30 0.855434782608695 7 31 3.5663043478260876 7 32 1.6619220151828848 7 33 3.4007418909592815 7 34 -0.142598343685302 7 35 -1.475810904071773 7 36 1.9610938578329877 7 37 3.642563837129054 7 38 3.3435990338164236 7 39 0.0415631469979294 7 40 3.2972049689441008 8 1 -6.939820565907523 8 2 -4.0076086956521735 8 3 -3.4348516218081464 8 4 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19.97191166321601 11 8 8.725500345065564 11 9 8.782660455486543 11 10 8.765165631469982 11 11 0.9505175983436853 11 12 20.45320910973085 11 13 21.784937888198755 11 14 21.811145617667357 11 15 10.177173913043479 11 16 0.6307453416149063 11 17 0.14853347135955985 11 18 6.531780538302279 11 19 3.8222049689440976 11 20 11.124534161490681 11 21 4.230607315389925 11 22 7.3207211870255335 11 23 18.95303657694962 11 24 19.148447204968946 11 25 16.39794685990338 11 26 1.3781573498964805 11 27 8.484489302967564 11 28 9.335162180814354 11 29 8.820945479641132 11 30 7.855434782608695 11 31 23.066304347826087 11 32 23.161922015182885 11 33 22.90074189095928 11 34 19.8574016563147 11 35 23.02418909592823 11 36 20.21109385783299 11 37 22.392563837129053 11 38 21.093599033816425 11 39 28.79156314699793 11 40 28.0472049689441 12 1 13.393512767425811 12 2 20.325724637681162 12 3 21.565148378191854 12 4 19.656211180124224 12 5 21.584541062801932 12 6 6.982539682539681 12 7 20.97191166321601 12 8 19.725500345065562 12 9 21.782660455486543 12 10 14.765165631469982 12 11 10.950517598343685 12 12 -0.5467908902691505 12 13 12.784937888198757 12 14 17.811145617667357 12 15 6.177173913043479 12 16 9.630745341614906 12 17 -1.8514665286404401 12 18 18.53178053830228 12 19 13.822204968944098 12 20 6.124534161490681 12 21 8.230607315389925 12 22 14.320721187025534 12 23 17.95303657694962 12 24 5.148447204968944 12 25 16.39794685990338 12 26 4.3781573498964805 12 27 10.484489302967564 12 28 10.335162180814354 12 29 9.820945479641132 12 30 21.855434782608697 12 31 23.066304347826087 12 32 20.161922015182885 12 33 24.90074189095928 12 34 24.8574016563147 12 35 14.024189095928227 12 36 21.21109385783299 12 37 25.392563837129053 12 38 25.093599033816425 12 39 21.79156314699793 12 40 18.0472049689441 13 1 1.060179434092479 13 2 1.6590579710144944 13 3 0.23181504485851967 13 4 -0.34378881987577614 13 5 -3.0821256038647338 13 6 -3.350793650793652 13 7 2.3052449965493427 13 8 2.725500345065564 13 9 7.115993788819875 13 10 3.7651656314699817 13 11 10.283850931677017 13 12 4.119875776397516 13 13 0.4516045548654226 13 14 2.477812284334025 13 15 -0.4894927536231872 13 16 5.96407867494824 13 17 2.815200138026226 13 18 8.198447204968947 13 19 8.488871635610765 13 20 7.457867494824013 13 21 7.563940648723259 13 22 4.6540545203588675 13 23 7.2863699102829536 13 24 10.815113871635612 13 25 6.731280193236715 13 26 6.3781573498964805 13 27 4.15115596963423 13 28 15.001828847481022 13 29 6.154278812974465 13 30 9.188768115942027 13 31 14.399637681159419 13 32 9.161922015182885 13 33 8.56740855762595 13 34 14.524068322981366 13 35 15.024189095928227 13 36 11.54442719116632 13 37 11.725897170462385 13 38 7.426932367149758 13 39 -0.5417701863354036 13 40 5.047204968944101 14 1 10.393512767425811 14 2 17.325724637681162 14 3 -7.434851621808146 14 4 -7.343788819875776 14 5 -7.415458937198068 14 6 -6.017460317460319 14 7 -9.028088336783991 14 8 -8.274499654934436 14 9 -8.217339544513457 14 10 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12.098498964803314 15 11 9.617184265010353 15 12 -0.8801242236024844 15 13 2.4516045548654226 15 14 -1.8555210489993073 15 15 1.843840579710145 15 16 4.630745341614906 15 17 3.815200138026226 15 18 5.198447204968945 15 19 3.8222049689440976 15 20 0.791200828157347 15 21 5.897273982056591 15 22 5.9873878536922 15 23 5.619703243616286 15 24 4.81511387163561 15 25 4.397946859903381 15 26 7.7114906832298145 15 27 7.817822636300898 15 28 7.668495514147688 15 29 6.487612146307797 15 30 8.188768115942029 15 31 6.066304347826088 15 32 7.495255348516219 15 33 5.9007418909592815 15 34 5.190734989648032 15 35 6.024189095928227 15 36 4.544427191166322 15 37 5.05923050379572 15 38 6.426932367149758 15 39 1.4582298136645955 15 40 4.380538302277435 16 1 7.5363699102829536 16 2 10.611438923395445 16 3 12.565148378191854 16 4 14.513354037267082 16 5 -1.9868875086266389 16 6 13.268253968253966 16 7 10.686197377501724 16 8 14.868357487922706 16 9 14.6398033126294 16 10 12.050879917184266 16 11 13.6648033126294 16 12 15.738923395445134 16 13 13.784937888198757 16 14 16.382574189095934 16 15 15.177173913043479 16 16 17.630745341614904 16 17 16.577104899930987 16 18 14.388923395445136 16 19 6.250776397515526 16 20 12.267391304347823 16 21 2.6591787439613537 16 22 7.3207211870255335 16 23 4.95303657694962 16 24 4.577018633540373 16 25 4.969375431331952 16 26 9.521014492753622 16 27 7.198775017253277 16 28 9.763733609385781 16 29 4.963802622498274 16 30 5.712577639751553 16 31 4.780590062111801 16 32 3.304779158040027 16 33 4.757884748102139 16 34 4.00025879917184 16 35 5.309903381642513 16 36 6.782522429261559 16 37 5.392563837129054 16 38 6.5221704623878525 16 39 7.505848861283643 16 40 6.047204968944101 17 1 3.393512767425811 17 2 -3.9242753623188396 17 3 5.565148378191854 17 4 -1.0937888198757761 17 5 1.3345410628019323 17 6 3.982539682539681 17 7 6.221911663216009 17 8 4.975500345065564 17 9 4.282660455486543 17 10 5.015165631469982 17 11 13.950517598343685 17 12 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14.656211180124224 106 5 -2.4154589371980677 106 6 9.98253968253968 106 7 17.97191166321601 106 8 18.725500345065562 106 9 15.782660455486543 106 10 17.765165631469984 106 11 14.950517598343685 106 12 2.4532091097308495 106 13 14.784937888198757 106 14 19.811145617667357 106 15 12.177173913043479 106 16 14.630745341614906 106 17 14.14853347135956 106 18 4.531780538302279 106 19 15.822204968944098 106 20 16.12453416149068 106 21 19.230607315389925 106 22 19.320721187025534 106 23 18.95303657694962 106 24 19.148447204968946 106 25 19.39794685990338 106 26 24.37815734989648 106 27 27.484489302967564 106 28 27.335162180814354 106 29 27.820945479641132 106 30 16.855434782608697 106 31 16.066304347826087 106 32 27.161922015182885 106 33 26.90074189095928 106 34 27.8574016563147 106 35 27.02418909592823 106 36 18.21109385783299 106 37 10.392563837129053 106 38 20.093599033816425 106 39 14.79156314699793 106 40 20.0472049689441 107 1 0.5935127674258105 107 2 4.125724637681161 107 3 4.765148378191853 107 4 2.2562111801242235 107 5 -0.2154589371980684 107 6 3.382539682539681 107 7 6.57191166321601 107 8 0.7255003450655639 107 9 1.3826604554865423 107 10 -2.2348343685300183 107 11 -1.449482401656315 107 12 2.85320910973085 107 13 -0.015062111801244171 107 14 -1.7888543823326408 107 15 2.9771739130434796 107 16 -3.1692546583850936 107 17 -0.4514665286404398 107 18 -1.4682194616977213 107 19 -3.577795031055903 107 20 0.32453416149068026 107 21 1.6306073153899252 107 22 -0.4792788129744663 107 23 -1.2469634230503805 107 24 -0.6515527950310558 107 25 -1.6020531400966194 107 26 0.5781573498964798 107 27 0.6844893029675632 107 28 0.9351621808143538 107 29 3.0209454796411306 107 30 0.855434782608695 107 31 1.8663043478260883 107 32 -0.4380779848171148 107 33 -0.4992581090407189 107 34 -1.3425983436853022 107 35 -0.3758109040717734 107 36 2.411093857832987 107 37 2.5925638371290534 107 38 2.293599033816423 107 39 2.59156314699793 107 40 2.2472049689441 109 1 11.393512767425811 109 2 9.82572463768116 109 3 13.565148378191854 109 4 5.656211180124224 109 5 4.084541062801932 109 6 13.98253968253968 109 7 16.97191166321601 109 8 16.725500345065562 109 9 14.282660455486543 109 10 10.765165631469982 109 11 6.450517598343685 109 12 7.9532091097308495 109 13 1.7849378881987565 109 14 11.811145617667359 109 15 15.177173913043479 109 16 0.13074534161490625 109 17 4.14853347135956 109 18 -1.4682194616977213 109 19 -1.1777950310559024 109 20 2.624534161490681 109 21 -0.7693926846100752 109 22 5.8207211870255335 109 23 0.9530365769496196 109 24 5.648447204968944 109 25 2.8979468599033806 109 26 2.3781573498964805 109 27 4.984489302967564 109 28 5.335162180814354 109 29 3.8209454796411313 109 30 3.855434782608695 109 31 12.566304347826087 109 32 12.661922015182885 109 33 11.400741890959281 109 34 11.857401656314698 109 35 13.024189095928227 109 36 4.211093857832988 109 37 1.892563837129054 109 38 3.5935990338164236 109 39 4.791563146997929 109 40 6.047204968944101 111 1 12.393512767425811 111 2 16.325724637681162 111 3 14.565148378191854 111 4 19.656211180124224 111 5 16.584541062801932 111 6 9.98253968253968 111 7 15.971911663216009 111 8 19.725500345065562 111 9 17.782660455486543 111 10 18.765165631469984 111 11 12.950517598343685 111 12 16.45320910973085 111 13 9.784937888198757 111 14 15.811145617667359 111 15 12.177173913043479 111 16 6.630745341614906 111 17 0.14853347135955985 111 18 13.531780538302279 111 19 -1.1777950310559024 111 20 -1.875465838509319 111 21 -1.7693926846100752 111 22 7.3207211870255335 111 23 8.95303657694962 111 24 14.148447204968944 111 25 9.397946859903382 111 26 2.3781573498964805 111 27 17.484489302967564 111 28 9.335162180814354 111 29 -0.1790545203588687 111 30 7.855434782608695 111 31 14.066304347826087 111 32 12.161922015182885 111 33 21.90074189095928 111 34 16.8574016563147 111 35 21.02418909592823 111 36 27.21109385783299 111 37 20.392563837129053 111 38 18.093599033816425 111 39 10.79156314699793 111 40 22.0472049689441 112 1 -3.106487232574189 112 2 3.8257246376811604 112 3 -5.434851621808146 112 4 -5.343788819875776 112 5 -6.415458937198068 112 6 -3.017460317460319 112 7 -2.5280883367839913 112 8 -5.774499654934436 112 9 -5.717339544513457 112 10 2.2651656314699817 112 11 4.450517598343685 112 12 6.4532091097308495 112 13 3.2849378881987565 112 14 4.811145617667359 112 15 7.677173913043479 112 16 3.1307453416149063 112 17 8.14853347135956 112 18 10.031780538302279 112 19 6.822204968944098 112 20 9.624534161490681 112 21 -3.769392684610075 112 22 -3.6792788129744665 112 23 -4.04696342305038 112 24 -3.851552795031056 112 25 -3.6020531400966194 112 26 -3.6218426501035195 112 27 -3.515510697032436 112 28 -3.664837819185646 112 29 -3.1790545203588687 112 30 -3.144565217391305 112 31 -3.9336956521739124 112 32 -3.838077984817115 112 33 -4.0992581090407185 112 34 -4.142598343685302 112 35 -3.975810904071773 112 36 -3.7889061421670123 112 37 -3.607436162870946 112 38 -3.9064009661835764 112 39 -3.2084368530020706 112 40 -3.9527950310558992 113 1 10.393512767425811 113 2 -8.67427536231884 113 3 -7.434851621808146 113 4 -7.343788819875776 113 5 -7.415458937198068 113 6 -6.017460317460319 113 7 -9.028088336783991 113 8 -8.274499654934436 113 9 -8.217339544513457 113 10 -7.234834368530018 113 11 -7.049482401656315 113 12 -6.5467908902691505 113 13 -7.2150621118012435 113 14 -7.188854382332641 113 15 -6.822826086956521 113 16 -5.369254658385094 113 17 -4.85146652864044 113 18 -4.468219461697721 113 19 -4.177795031055902 113 20 -4.875465838509319 113 21 -3.769392684610075 113 22 -3.6792788129744665 113 23 -4.04696342305038 113 24 -3.851552795031056 113 25 -3.6020531400966194 113 26 -3.6218426501035195 113 27 -3.515510697032436 113 28 -3.664837819185646 113 29 -3.1790545203588687 113 30 -3.144565217391305 113 31 -3.9336956521739124 113 32 -3.838077984817115 113 33 -4.0992581090407185 113 34 -4.142598343685302 113 35 -3.975810904071773 113 36 -3.7889061421670123 113 37 -3.607436162870946 113 38 -3.9064009661835764 113 39 -3.2084368530020706 113 40 -3.9527950310558992 118 1 10.393512767425811 118 2 -8.67427536231884 118 3 -7.434851621808146 118 4 -7.343788819875776 118 5 -7.415458937198068 118 6 -6.017460317460319 118 7 -9.028088336783991 118 8 -8.274499654934436 118 9 -8.217339544513457 118 10 -7.234834368530018 118 11 -7.049482401656315 118 12 -6.5467908902691505 118 13 -7.2150621118012435 118 14 -7.188854382332641 118 15 -6.822826086956521 118 16 -5.369254658385094 118 17 -4.85146652864044 118 18 -4.468219461697721 118 19 -4.177795031055902 118 20 -4.875465838509319 118 21 -3.769392684610075 118 22 -3.6792788129744665 118 23 -4.04696342305038 118 24 -3.851552795031056 118 25 -3.6020531400966194 118 26 -3.6218426501035195 118 27 -3.515510697032436 118 28 -3.664837819185646 118 29 -3.1790545203588687 118 30 -3.144565217391305 118 31 -3.9336956521739124 118 32 -3.838077984817115 118 33 -4.0992581090407185 118 34 -4.142598343685302 118 35 -3.975810904071773 118 36 -3.7889061421670123 118 37 -3.607436162870946 118 38 -3.9064009661835764 118 39 -3.2084368530020706 118 40 -3.9527950310558992 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 919.0 5 29.0 6 28.0 7 25.0 8 20.0 9 40.0 10 63.0 11 75.0 12 46.0 13 41.0 14 33.0 15 25.0 16 22.0 17 14.0 18 10.0 19 25.0 20 45.0 21 61.0 22 61.0 23 54.0 24 46.0 25 39.0 26 20.0 27 16.0 28 12.0 29 11.0 30 5.0 31 3.0 32 0.0 33 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 97.98770262716602 1.1179429849077696 0.3912800447177194 0.5030743432084964 8 97.7069351230425 0.9507829977628635 0.9507829977628635 0.39149888143176736 9 96.75796534376747 1.4533258803801008 1.1738401341531581 0.6148686416992734 10 50.5586592178771 33.63128491620112 8.491620111731843 7.318435754189945 11 31.955307262569832 27.76536312849162 25.921787709497206 14.35754189944134 12 30.726256983240223 22.960893854748605 26.81564245810056 19.497206703910614 13 23.180291153415453 23.180291153415453 28.499440089585665 25.139977603583425 14 24.35754189944134 28.60335195530726 29.27374301675978 17.76536312849162 15 26.70391061452514 28.435754189944134 30.05586592178771 14.804469273743019 16 30.446927374301673 29.329608938547487 25.58659217877095 14.63687150837989 17 28.49162011173184 27.988826815642458 29.27374301675978 14.24581005586592 18 26.31284916201117 30.558659217877093 27.76536312849162 15.363128491620111 19 23.687150837988828 32.68156424581006 25.58659217877095 18.044692737430168 20 24.41340782122905 29.608938547486037 31.731843575418996 14.24581005586592 21 22.68156424581006 29.832402234636874 30.782122905027933 16.70391061452514 22 23.18435754189944 26.59217877094972 31.340782122905026 18.88268156424581 23 22.849162011173185 28.156424581005584 29.553072625698324 19.441340782122904 24 21.787709497206702 31.28491620111732 29.16201117318436 17.76536312849162 25 22.801120448179272 31.540616246498598 24.985994397759104 20.672268907563023 26 24.465691788526435 31.439820022497187 25.196850393700785 18.89763779527559 27 20.751542344363433 30.959057767807067 25.014021312394842 23.27537857543466 28 19.5858981533296 31.001678791270287 27.700055959709008 21.7123670956911 29 25.15440763615946 31.274564851207188 26.052779337450872 17.51824817518248 30 22.930648769574944 29.697986577181208 28.97091722595078 18.400447427293066 31 24.972067039106143 27.318435754189945 27.48603351955307 20.22346368715084 32 26.221224031443008 28.411005053340823 28.24256035934868 17.12521055586749 33 26.033519553072626 27.20670391061453 28.71508379888268 18.044692737430168 34 22.17877094972067 28.994413407821227 30.558659217877093 18.268156424581004 35 17.6536312849162 32.3463687150838 32.122905027932966 17.877094972067038 36 19.12751677852349 33.557046979865774 27.237136465324387 20.078299776286354 37 21.731843575418992 32.3463687150838 29.944134078212294 15.977653631284916 38 22.51396648044693 33.18435754189944 27.150837988826815 17.150837988826815 39 21.620111731843576 31.843575418994412 26.368715083798882 20.16759776536313 40 21.40860816098379 29.681386249301283 27.277808831749578 21.632196757965342 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 4.0 11 9.0 12 14.0 13 14.0 14 16.0 15 18.0 16 15.5 17 13.0 18 13.0 19 15.5 20 18.0 21 14.5 22 11.0 23 11.0 24 15.0 25 19.0 26 30.5 27 42.0 28 42.0 29 48.5 30 55.0 31 59.0 32 63.0 33 63.0 34 80.0 35 97.0 36 107.0 37 117.0 38 117.0 39 119.0 40 121.0 41 124.0 42 127.0 43 127.0 44 143.5 45 160.0 46 148.5 47 137.0 48 137.0 49 153.5 50 170.0 51 160.5 52 151.0 53 151.0 54 147.0 55 143.0 56 132.5 57 122.0 58 122.0 59 109.0 60 96.0 61 73.5 62 51.0 63 51.0 64 39.5 65 28.0 66 17.5 67 7.0 68 7.0 69 4.5 70 2.0 71 2.0 72 2.0 73 2.0 74 1.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.055865921787709494 8 0.11173184357541899 9 0.055865921787709494 10 0.0 11 0.0 12 0.0 13 0.22346368715083798 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.27932960893854747 26 0.6703910614525139 27 0.3910614525139665 28 0.16759776536312848 29 0.5027932960893855 30 0.11173184357541899 31 0.0 32 0.5027932960893855 33 0.0 34 0.0 35 0.0 36 0.11173184357541899 37 0.0 38 0.0 39 0.0 40 0.055865921787709494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1790.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.89944134078212 #Duplication Level Percentage of deduplicated Percentage of total 1 94.83282674772036 87.15083798882681 2 3.708206686930091 6.815642458100559 3 0.851063829787234 2.346368715083799 4 0.12158054711246201 0.44692737430167595 5 0.12158054711246201 0.5586592178770949 6 0.12158054711246201 0.6703910614525139 7 0.060790273556231005 0.3910614525139665 8 0.060790273556231005 0.44692737430167595 9 0.0 0.0 >10 0.12158054711246201 1.1731843575418994 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 11 0.6145251396648044 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 10 0.5586592178770949 No Hit TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.44692737430167595 No Hit TCAAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 7 0.3910614525139665 No Hit TCAAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 6 0.33519553072625696 No Hit TCAAAGGGGATTGGGCGGATTGAACAAAAACTCATCTCAG 6 0.33519553072625696 No Hit TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 5 0.27932960893854747 No Hit TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 5 0.27932960893854747 No Hit TCAAAGGGGAGCCAGTGGCCTGAACTTTGAAACCAGCAGC 4 0.22346368715083798 No Hit TCAAAGGGATGGAAGATATATGTCTGATGTATCTTCTCAC 4 0.22346368715083798 No Hit TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.16759776536312848 No Hit TCAAAGGGGGGGAGTTGACTGTCTAAAAGCTACCAGAGCA 3 0.16759776536312848 No Hit TCAAAGGGGGGTTTCGACGCTAGAGGTAACCTCAGCATTC 3 0.16759776536312848 No Hit TCAAAGGGGGTGCGTTGCCGTGGCTGATGGGCGCTGTCTG 3 0.16759776536312848 No Hit TCAAAGGGGATAATAATTCGTTTTATGCATCATTAAGTAG 3 0.16759776536312848 No Hit TCAAAGGGGGATTCTTAAAGGGGCCCAAGAGATCGCACGG 3 0.16759776536312848 No Hit TCAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.16759776536312848 No Hit TCAAAGGGGAGTCTTTTGCCTTGCCTTTATTACATGTAAA 3 0.16759776536312848 No Hit TCAAAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.16759776536312848 No Hit TCAAAGGGGTGTTGATTTTTTTGATGATAGACTATATACA 3 0.16759776536312848 No Hit TCAAAGGGGGACTTGGGTTCCGGCCTATGCAAGTCTGCAC 3 0.16759776536312848 No Hit TCAAAGGGGGTTTTATCCAACTCATCCCTTGACATCGTGC 3 0.16759776536312848 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC 3 0.16759776536312848 No Hit TCAAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 3 0.16759776536312848 No Hit TCAAAGGGGGGCCTGGTGAGATGGCTCAGTGGGTAAGAGC 2 0.11173184357541899 No Hit TCAAAGGGGATTGTTTGGTTCCAGAAAACCAACCACCTTT 2 0.11173184357541899 No Hit TCAAAGGGGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11173184357541899 No Hit TCAAAGGGGGTTTGGGTCAGATTCTAGCTTTTGTCACGTT 2 0.11173184357541899 No Hit TCAAAGGGGCTTCGTAGGAGTAAACATAACATTCTTCCCT 2 0.11173184357541899 No Hit TCAAAGGGGAGGGCAGGAGTTGGGGAAAGACGCGCAGAAA 2 0.11173184357541899 No Hit TCAAAGAGGGAGCCTTAGGCTTTATTTTCTTATTTACAGT 2 0.11173184357541899 No Hit TCAAAGGGGAACTTTATTCTTTTTTTTCTCCTGCTGAAAC 2 0.11173184357541899 No Hit TCAAAGGGGGAAGTTTCATTACCTGATCCGTGGGGCTTTG 2 0.11173184357541899 No Hit TCAAAGGGGATGTCTCTTCCTGTCTGTTTCTTTACCTCTC 2 0.11173184357541899 No Hit TCAAAGGGGAGTATTTAGCTGAGGGCAGTTATGTTTCTCC 2 0.11173184357541899 No Hit TCAAAGGGGGCCACTGCTCTTTCAAATTCAAAATGTTATC 2 0.11173184357541899 No Hit TCAAAGGGGGATCCTGTGGATAAAATTGTCTTGCAAAAAT 2 0.11173184357541899 No Hit TCAAAGGGGGTGCAGTGCTTCAAAGGGGTCACAGATCCTC 2 0.11173184357541899 No Hit TCAAAGGGGATGAGTGGACCAGCGGGTCCAACACAGGGAA 2 0.11173184357541899 No Hit TCAAAGGGGCGGAATTGTTTTATCCAACTCATCCCTTGAC 2 0.11173184357541899 No Hit TCAAAGGGGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11173184357541899 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 2 0.11173184357541899 No Hit TCAAAGGGGGGGTTCGTGTGTTGTTCGGGATGCCACTGGC 2 0.11173184357541899 No Hit TCAAAGGGGAAACAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11173184357541899 No Hit TCAAAGGGGGAGAGGCTCAGAGGATTCTGACAGCTTCTTT 2 0.11173184357541899 No Hit TCAAAGGGGACAATTTGCTGTGCCTTTATTCCAGAGTCGG 2 0.11173184357541899 No Hit TCAAAGGGGAAGTTTAACGGCCGCGGTATCCTGACCGTGC 2 0.11173184357541899 No Hit TCAAAGGGGTACAGTTGGTGCTCTAACCGGAATTGTTTTA 2 0.11173184357541899 No Hit TCAAAGGGGATCAGTGGGTGGAGGGAAGGAAGAGAGGCTA 2 0.11173184357541899 No Hit TCAAAGGGGGTTTCCCCACCAGCATGATTGGCGTGGGCCT 2 0.11173184357541899 No Hit TCAAAGGGGATTAGAAAAATTATTTAGATACTTGACCATT 2 0.11173184357541899 No Hit TCAAAGGGGATTGTTTTCCTGGTTTCACTGAGTTGCCGCA 2 0.11173184357541899 No Hit TCAAAGGGGGATCTGGTGTACTCCACTCGTCTGGTGTTCG 2 0.11173184357541899 No Hit TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA 2 0.11173184357541899 No Hit TCAAAGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAG 2 0.11173184357541899 No Hit TCAAAGGGGGAGAACTTAAGCTGTGGAGACTGAGACCACC 2 0.11173184357541899 No Hit TCAAAGGGGATTGCATACTATTTTAAAGATGTTTTAATGT 2 0.11173184357541899 No Hit TCAAAGGGGGAGCTGGAGAGATGGCTCAGTGGGTAAGAGC 2 0.11173184357541899 No Hit TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 2 0.11173184357541899 No Hit TCAAAGGGGTGAGGTGTGGGTGTGTTCTATATTCCAAATT 2 0.11173184357541899 No Hit TCAAAGGGGTGGCATAATACACACCAAAACCACCCAGATT 2 0.11173184357541899 No Hit TCAAAGGGGGCTTTGGGACTCTGTTAACTTTGGGGAAGGA 2 0.11173184357541899 No Hit TCAAAGGGGACCCAAGGGATGGTTTCAGGGACTGAGGTGT 2 0.11173184357541899 No Hit TCAAAGGGGAACCCTGGGTTGATTAGCATATGCTTCTAAA 2 0.11173184357541899 No Hit TCAAAGGGGAAGATTGCATTTTTATCTAAGGAATGATGTT 2 0.11173184357541899 No Hit TCAAAGGGGGATCTGGAGAGATCGCTTAGTAATCAAATGC 2 0.11173184357541899 No Hit TCAAAGGGGGCTTTTTAAAGAAAAATATATATATTGTTTC 2 0.11173184357541899 No Hit TCAAAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 2 0.11173184357541899 No Hit TCAAAGGGGAGTGCAGTGCTTCAAAGGGGTCACAGATCCT 2 0.11173184357541899 No Hit TCAAAGGGGAGGATTAGATGTAGACACACGAGCTTACTTT 2 0.11173184357541899 No Hit TCAAAGGGGATTGGTAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.11173184357541899 No Hit TCAAAGGGGGACGCTACTAAGACTACCCAGATGCTTACAC 2 0.11173184357541899 No Hit TCAAAGGGGGGGGTTCGATTCCTTTATTTTCTTATTTACA 2 0.11173184357541899 No Hit TCAAAGGGGGATCCAAAGGGTTTTGGAGGGTTTCTATAGG 2 0.11173184357541899 No Hit TCAAAGGGGCATTCTGTATTGGAAATGCTGTGAAACTGTA 2 0.11173184357541899 No Hit TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 2 0.11173184357541899 No Hit TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGC 2 0.11173184357541899 No Hit TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.11173184357541899 No Hit TCAAAGGGGATTTTATGTGTATGAGTGTTTTGCTTGCATG 2 0.11173184357541899 No Hit TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.11173184357541899 No Hit TCAAAGGGGGGGGCTGGAGAGATGGCTCGGCGGTTAAGAG 2 0.11173184357541899 No Hit TCAAAGGGGGAGTGCAGTGCTTCAAAGGGGAGTCGAAGTG 2 0.11173184357541899 No Hit TCAAAGGGGTCTTATGTGGAATCAGAAATTCTTATTAATG 2 0.11173184357541899 No Hit TCAAAGGGGGTGCGTTGCCGTGGATGATGGGCGCTGTCTG 2 0.11173184357541899 No Hit TCAAAGGGGAACAGGAACTTTTTTTTTTGAAGATTTGAAA 2 0.11173184357541899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.055865921787709494 0.0 0.0 0.0 0.0 15 0.055865921787709494 0.0 0.0 0.0 0.0 16 0.055865921787709494 0.0 0.0 0.0 0.0 17 0.055865921787709494 0.0 0.0 0.0 0.0 18 0.055865921787709494 0.0 0.0 0.0 0.0 19 0.055865921787709494 0.0 0.0 0.0 0.0 20 0.055865921787709494 0.0 0.0 0.0 0.0 21 0.055865921787709494 0.0 0.0 0.0 0.0 22 0.055865921787709494 0.0 0.0 0.0 0.0 23 0.055865921787709494 0.0 0.0 0.0 0.0 24 0.055865921787709494 0.0 0.0 0.0 0.0 25 0.055865921787709494 0.0 0.0 0.0 0.0 26 0.055865921787709494 0.0 0.0 0.0 0.0 27 0.16759776536312848 0.0 0.0 0.0 0.0 28 0.16759776536312848 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGT 20 0.0020983247 34.0 5 AGGGGGC 25 1.318037E-4 34.0 5 AGGGGGA 25 1.318037E-4 34.0 5 AAGGGGA 30 8.064844E-6 34.0 4 TCAAAGG 170 0.0 33.0 1 AAAGGGG 170 0.0 33.0 3 CAAAGGG 170 0.0 33.0 2 AAGGGGG 115 0.0 32.521736 4 AGGGGGG 45 7.8429366E-8 30.222221 5 GGGGGGG 55 1.6455146E-5 21.636362 6 >>END_MODULE