FastQCFastQC Report
Fri 17 Jun 2016
SRR1525966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3465.623273200065009No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3465.623273200065009No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1342.1777994474240208No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT971.5764667641800747No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA600.9751340809361287No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC410.6663416219730214No Hit
AACGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC360.5850804485616773No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC330.5363237445148707No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT220.35754916300991385No Hit
AACGCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC200.32504469364537625No Hit
AACGCCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT190.3087924589631074No Hit
AACGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG170.2762879895985698No Hit
AACGCCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC160.260035754916301No Hit
AACGCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC150.24378352023403219No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT150.24378352023403219No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA150.24378352023403219No Hit
AACGCCGGGCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA140.22753128555176336No Hit
AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA140.22753128555176336No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT140.22753128555176336No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTGTTCAATCCGCCCAATC130.21127905086949453No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT130.21127905086949453No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA130.21127905086949453No Hit
AACGCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACT120.19502681618722573No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCCATTGAACAAAAACTC120.19502681618722573No Hit
AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG110.17877458150495693No Hit
AACGCCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA110.17877458150495693No Hit
AACGCCGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC100.16252234682268812No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCCATCCGCCCAATCT100.16252234682268812No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT100.16252234682268812Illumina Single End Adapter 2 (95% over 24bp)
AACGCCGGGGTGCAGTGCTAACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGG90.14627011214041932No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA90.14627011214041932No Hit
AACGCCGGGTCACAGAACCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT90.14627011214041932No Hit
AACGCCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT90.14627011214041932No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAAAAA80.1300178774581505No Hit
AACGCCGGGTCACAGGTCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC80.1300178774581505No Hit
AACGCCGGGTCACCCATCCTCTTCTGAAGGAGATTGGGCGGATTGACACAAAACTC80.1300178774581505No Hit
AACGCCGGGTCACAGAACCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC80.1300178774581505No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT80.1300178774581505Illumina Single End Adapter 1 (95% over 23bp)
AACGCCGGGTCACAGATCCTCTTAAGGAGATTGGGCGGATTGAACAAAAACTCATC80.1300178774581505No Hit
AACGCCGGGTCACCCATCCTCTTCTGAGATGAGTTTTTGTTCACTCCGCCCAATCT70.11376564277588168No Hit
AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11376564277588168No Hit
AACGCCGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT70.11376564277588168No Hit
AACGCCGGGTCACAGACCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC70.11376564277588168No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTGTTCAATCCGCCCAATCTC70.11376564277588168No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11376564277588168No Hit
AACGCCGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC70.11376564277588168No Hit
AACGCCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA70.11376564277588168No Hit
AACGCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATCGAACAAAAACTCATC70.11376564277588168No Hit
AACGCCGGGTCCCCCCTCCTCTTCTGAAGGAGATTGGGCGGATCGACACAAAACTC70.11376564277588168No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTT70.11376564277588168No Hit
AACGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT70.11376564277588168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCC203.794708E-450.1803337
AATCCTC203.794708E-450.1803315
AAATCCT203.794708E-450.1803314
CTCCTCT251.8123364E-550.1803316
GGCCGAT150.00792353350.1803337
TTTTTGC150.00792353350.1803334
TTGGGCC150.00792353350.1803334
CCATCCG150.00792353350.1803342
TCCATCC150.00792353350.1803341
TGGGCCG150.00792353350.1803335
GCTCCTC203.794708E-450.1803315
GCCGATT150.00792353350.1803338
CCGATTG150.00792353350.1803339
TTTGCTC150.00792353350.1803336
CGATTGA150.00792353350.1803340
GGGCCGA150.00792353350.1803336
GTCACAA203.9533508E-449.772369
CGGGGAG203.9533508E-449.772366
GCCGGGC402.0954758E-949.772364
CCGGGGA203.9533508E-449.772365
Produced by FastQC (version 0.11.5)