##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525966_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6153 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.185437997724684 38.0 36.0 39.0 33.0 40.0 2 35.50983260198277 38.0 36.0 39.0 29.0 40.0 3 34.498130993011536 38.0 34.0 39.0 23.0 40.0 4 34.15049569315781 38.0 33.0 39.0 23.0 40.0 5 34.592881521209165 38.0 34.0 39.0 24.0 40.0 6 34.94376726799935 38.0 35.0 39.0 26.0 40.0 7 34.84706647163985 38.0 35.0 39.0 27.0 40.0 8 35.052169673330084 38.0 35.0 39.0 28.0 40.0 9 35.026166097838455 38.0 35.0 39.0 27.0 40.0 10 33.74321469202015 37.0 33.0 39.0 23.0 40.0 11 33.99317406143345 38.0 33.0 39.0 23.0 40.0 12 33.592881521209165 38.0 33.0 39.0 22.0 40.0 13 31.950755728912725 36.0 30.0 39.0 15.0 40.0 14 33.62587355761417 38.0 33.0 39.0 22.0 40.0 15 30.149032992036403 35.0 27.0 38.0 10.0 39.0 16 31.493742889647326 35.0 29.0 38.0 15.0 40.0 17 29.736551275800423 34.0 24.0 38.0 10.0 39.0 18 30.16398504794409 35.0 25.0 38.0 12.0 40.0 19 31.201040143019664 35.0 27.0 39.0 16.0 40.0 20 31.488867219242646 35.0 28.0 38.0 17.0 40.0 21 33.22119291402568 38.0 32.0 39.0 18.0 40.0 22 32.66699171136031 37.0 31.0 39.0 16.0 40.0 23 33.66065333983423 38.0 33.0 39.0 20.0 40.0 24 32.60588330895498 37.0 31.0 39.0 16.0 40.0 25 33.195676905574516 38.0 33.0 39.0 19.0 40.0 26 32.60377051844629 36.0 32.0 39.0 18.0 40.0 27 33.11019015114578 37.0 33.0 39.0 20.0 40.0 28 33.3359336908825 38.0 33.0 39.0 20.0 40.0 29 32.70258410531448 37.0 32.0 39.0 16.0 40.0 30 32.6411506582155 37.0 32.0 39.0 15.0 40.0 31 32.43474727775069 36.0 32.0 39.0 14.0 40.0 32 32.71509832601983 37.0 32.0 39.0 14.0 40.0 33 32.139281651227044 36.0 32.0 39.0 10.0 39.0 34 31.54786283113928 36.0 31.0 38.0 10.0 39.0 35 32.48009101251422 37.0 32.0 39.0 4.0 40.0 36 30.901023890784984 35.0 30.0 38.0 4.0 40.0 37 30.815374614009425 35.0 30.0 38.0 4.0 40.0 38 30.480416057207865 35.0 30.0 38.0 4.0 39.0 39 28.62684869169511 34.0 25.0 38.0 4.0 39.0 40 26.56411506582155 33.0 14.0 37.0 4.0 39.0 41 23.135868681943766 28.0 7.0 35.0 4.0 39.0 42 23.845766292865267 28.0 11.0 35.0 4.0 39.0 43 25.289289777344386 30.0 12.0 36.0 4.0 39.0 44 25.229969120754102 31.0 10.0 36.0 4.0 39.0 45 25.356411506582155 31.0 9.0 36.0 4.0 39.0 46 22.89598569803348 27.0 4.0 35.0 4.0 39.0 47 22.661953518608808 27.0 4.0 35.0 4.0 38.0 48 22.615959694457988 26.0 4.0 35.0 4.0 39.0 49 22.240370550950757 27.0 4.0 35.0 4.0 39.0 50 22.98797334633512 28.0 4.0 35.0 4.0 39.0 51 23.3287827076223 29.0 4.0 35.0 4.0 39.0 52 23.19096375751666 29.0 4.0 35.0 4.0 39.0 53 23.24735901186413 29.0 4.0 35.0 4.0 39.0 54 22.892247684056557 29.0 4.0 35.0 4.0 39.0 55 23.294002925402243 30.0 4.0 36.0 4.0 39.0 56 21.88152120916626 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.975020614306324 1 2 4.788119793371891 1 3 5.741081887300368 1 4 5.985444783974195 1 5 5.767732850716037 1 6 5.601891968908774 1 7 4.556921556501386 1 8 5.373208677410361 1 9 4.795502443401588 1 10 4.300403191579662 1 11 -0.34223416639382975 1 12 -7.311893848448474 1 13 -15.355984818169693 1 14 -6.57983648004657 1 15 1.267390895752225 1 16 -5.560311094134629 1 17 -6.958594043678069 1 18 -5.032671553469871 1 19 -3.4776422690288236 1 20 -2.7021951204724353 1 21 4.893836322407754 1 22 5.651447548716458 1 23 5.222001831035431 1 24 5.626433906896093 1 25 5.580875380455211 1 26 5.5103107909830555 1 27 6.150690579262012 1 28 5.917624625607818 1 29 5.448659160633962 1 30 6.712498332666385 1 31 4.5033974195738935 1 32 3.7636715292177456 1 33 6.128857906799077 1 34 2.908516740029345 1 35 3.3256211575539254 1 36 -3.7017185662143604 1 37 -3.2217871997283787 1 38 -2.7914750748784556 1 39 -2.4314807741278344 1 40 -15.393129069809742 1 41 -6.698021329744019 1 42 -7.03570185650017 1 43 -8.246514969624213 1 44 -16.734394970109253 1 45 -9.402154195011345 1 46 -9.94657378102757 1 47 -11.17720936859592 1 48 -9.577005044442014 1 49 -14.545369359865159 1 50 -8.525734233087176 1 51 -8.013474480701372 1 52 -9.188010658808977 1 53 -9.134064838056442 1 54 -7.798917142614622 1 55 -8.64686571598336 1 56 -4.655356384978235 2 1 4.544465058750767 2 2 5.329786460038555 2 3 6.866081887300368 2 4 6.860444783974195 2 5 6.670510628493815 2 6 6.393558635575438 2 7 5.987477112056943 2 8 6.039875344077025 2 9 6.14272466562381 2 10 7.189292080468547 2 11 4.379988055828392 2 12 0.7575505959959692 2 13 1.435681848496973 2 14 5.059052408842319 2 15 4.892390895752225 2 16 3.7730222391987027 2 17 7.221961511877488 2 18 5.425661779863461 2 19 7.175135508748955 2 20 3.881138212860897 2 21 5.893836322407754 2 22 6.054225326494237 2 23 5.0831129421465455 2 24 6.640322795784979 2 25 5.803097602677433 2 26 6.843644124316391 2 27 6.761801690373126 2 28 6.778735736718932 2 29 7.393103605078405 2 30 6.420831665999721 2 31 6.322841864018336 2 32 5.777560418106631 2 33 7.114969017910191 2 34 6.922405628918231 2 35 7.49228782422059 2 36 7.1177258782300825 2 37 8.597657244716064 2 38 8.402969369565987 2 39 8.971297003649944 2 40 7.204093152412479 2 41 7.81586755914487 2 42 6.255964810166496 2 43 5.809040585931342 2 44 -1.4427283034425855 2 45 5.4589569160997655 2 46 5.03953733008354 2 47 2.767235075848525 2 48 4.936883844446875 2 49 0.42685286235706243 2 50 5.0437102113572685 2 51 7.639303297076406 2 52 7.464767118968801 2 53 6.379824050832447 2 54 7.131638412940934 2 55 6.964245395127751 2 56 8.27519917057732 3 1 4.336131725417431 3 2 5.454786460038555 3 3 6.741081887300368 3 4 4.985444783974195 3 5 5.96217729516048 3 6 5.935225302242102 3 7 5.362477112056943 3 8 5.039875344077025 3 9 4.684391332290474 3 10 5.855958747135212 3 11 5.129988055828392 3 12 6.215883929329305 3 13 7.727348515163637 3 14 5.892385742175655 3 15 1.1007242290855572 3 16 5.189688905865367 3 17 7.930294845210824 3 18 7.800661779863461 3 19 7.466802175415619 3 20 6.464471546194233 3 21 7.22716965574109 3 22 7.679225326494237 3 23 6.74977960881321 3 24 7.265322795784979 3 25 6.803097602677433 3 26 5.676977457649727 3 27 5.095135023706462 3 28 6.278735736718932 3 29 6.143103605078405 3 30 6.629164999333057 3 31 6.697841864018336 3 32 4.069227084773296 3 33 6.656635684576855 3 34 5.047405628918231 3 35 5.908954490887254 3 36 7.9927258782300825 3 37 8.3059905780494 3 38 9.236302702899323 3 39 9.67963033698328 3 40 9.495759819079144 3 41 14.69086755914487 3 42 12.964298143499832 3 43 12.892373919264678 3 44 10.26560502989075 3 45 13.12562358276643 3 46 14.581203996750205 3 47 14.517235075848525 3 48 15.061883844446875 3 49 15.926852862357062 3 50 12.668710211357268 3 51 13.68096996374307 3 52 13.006433785635465 3 53 12.754824050832447 3 54 13.58997174627427 3 55 12.964245395127751 3 56 16.316865837243984 4 1 3.237646876932587 4 2 4.091150096402188 4 3 5.104718250936735 4 4 3.8036266021560152 4 5 4.818237901221089 4 6 4.904922271939071 4 7 5.47611347569331 4 8 5.221693525895205 4 9 4.744997392896536 4 10 5.280201171377641 4 11 5.061806237646572 4 12 5.859823323268696 4 13 6.0379545757696995 4 14 5.672688772478686 4 15 6.085572713934045 4 16 8.863931330107796 4 17 9.778779693695668 4 18 10.846116325318008 4 19 9.663771872385322 4 20 8.90386548558817 4 21 5.257472686044121 4 22 7.338316235585143 4 23 4.424022033055639 4 24 7.515322795784979 4 25 5.894006693586526 4 26 2.8436441243163912 4 27 4.1708925994640325 4 28 3.756008463991659 4 29 5.984012695987499 4 30 6.045831665999721 4 31 3.1296600458365162 4 32 3.8343785999248112 4 33 4.3308781088192845 4 34 1.7974056289182307 4 35 3.7195605514933163 4 36 4.106362241866449 4 37 4.768111790170611 4 38 6.198423915020534 4 39 6.8008424581953975 4 40 -4.625452302132974 4 41 10.827231195508507 4 42 10.90369208289377 4 43 9.218131495022252 4 44 10.295908060193781 4 45 10.708956916099766 4 46 12.709991875538087 4 47 12.812689621303072 4 48 15.584611117174148 4 49 14.04048922599343 4 50 15.100528393175448 4 51 14.491576024349133 4 52 12.226130755332434 4 53 12.118460414468814 4 54 11.029365685668207 4 55 11.555154486036844 4 56 12.172926443304593 5 1 4.219465058750764 5 2 5.354786460038554 5 3 6.141081887300366 5 4 6.685444783974198 5 5 6.545510628493815 5 6 5.8685586355754396 5 7 6.212477112056945 5 8 6.039875344077025 5 9 6.2177246656238125 5 10 6.689292080468547 5 11 6.5799880558283945 5 12 5.932550595995966 5 13 8.410681848496974 5 14 4.509052408842322 5 15 9.567390895752222 5 16 6.873022239198704 5 17 10.296961511877491 5 18 9.80066177986346 5 19 8.100135508748952 5 20 8.8311382128609 5 21 5.193836322407751 5 22 7.529225326494238 5 23 5.933112942146547 5 24 7.415322795784977 5 25 6.903097602677434 5 26 6.943644124316393 5 27 6.761801690373126 5 28 6.028735736718932 5 29 6.493103605078407 5 30 6.4458316659997195 5 31 6.147841864018339 5 32 6.252560418106633 5 33 6.839969017910192 5 34 4.497405628918234 5 35 4.892287824220588 5 36 8.442725878230085 5 37 8.722657244716064 5 38 9.652969369565987 5 39 9.146297003649941 5 40 13.229093152412478 5 41 16.890867559144873 5 42 16.030964810166495 5 43 12.009040585931345 5 44 11.232271696557412 5 45 11.808956916099767 5 46 13.564537330083539 5 47 12.467235075848528 5 48 13.711883844446874 5 49 13.776852862357064 5 50 11.818710211357267 5 51 8.964303297076405 5 52 9.689767118968803 5 53 8.454824050832446 5 54 10.456638412940933 5 55 8.764245395127752 5 56 9.60019917057732 6 1 3.7527983920841024 6 2 5.232564237816334 6 3 6.852192998411482 6 4 5.874333672863081 6 5 6.1010661840493725 6 6 6.268558635575438 6 7 5.556921556501386 6 8 5.706542010743689 6 9 5.906613554512695 6 10 6.855958747135212 6 11 6.65776583360617 6 12 6.910328373773748 6 13 7.644015181830309 6 14 6.975719075508984 6 15 8.37850200686334 6 16 7.884133350309817 6 17 10.041405956321931 6 18 10.189550668752346 6 19 8.077913286526734 6 20 7.631138212860897 6 21 6.116058544629976 6 22 3.984780882049794 6 23 4.222001831035431 6 24 7.515322795784979 6 25 6.914208713788547 6 26 5.065866346538613 6 27 4.0395794681509045 6 28 2.917624625607818 6 29 4.004214716189519 6 30 3.601387221555278 6 31 4.947841864018336 6 32 3.43033819588441 6 33 4.795524573465748 6 34 3.464072295584895 6 35 3.214510046442811 6 36 7.076059211563418 6 37 7.3337683558271785 6 38 4.652969369565987 6 39 5.67963033698328 6 40 6.495759819079147 6 41 9.746423114700427 6 42 11.630964810166496 6 43 8.97570725259801 6 44 10.154493918779636 6 45 9.486734693877544 6 46 12.16453733008354 6 47 10.822790631404082 6 48 12.089661622224654 6 49 11.899075084579284 6 50 11.14093243357949 6 51 9.542081074854185 6 52 8.811989341191023 6 53 8.754824050832447 6 54 10.31219396849649 6 55 11.353134284016637 6 56 11.78908805946621 7 1 4.6194650587507695 7 2 5.654786460038558 7 3 6.541081887300365 7 4 6.485444783974195 7 5 6.545510628493815 7 6 6.468558635575441 7 7 5.812477112056946 7 8 5.9398753440770236 7 9 6.117724665623811 7 10 6.4892920804685446 7 11 5.679988055828389 7 12 5.932550595995966 7 13 7.910681848496974 7 14 6.009052408842322 7 15 8.467390895752228 7 16 8.0730222391987 7 17 10.19696151187749 7 18 10.20066177986346 7 19 9.800135508748955 7 20 8.231138212860898 7 21 7.9938363224077555 7 22 7.929225326494237 7 23 6.733112942146544 7 24 8.515322795784979 7 25 7.703097602677431 7 26 6.043644124316394 7 27 4.461801690373129 7 28 6.428735736718931 7 29 6.593103605078408 7 30 6.845831665999718 7 31 7.647841864018339 7 32 6.852560418106634 7 33 7.13996901791019 7 34 7.297405628918231 7 35 7.1922878242205925 7 36 8.54272587823008 7 37 7.822657244716066 7 38 8.952969369565984 7 39 10.046297003649947 7 40 8.42909315241248 7 41 15.890867559144873 7 42 14.130964810166496 7 43 12.70904058593134 7 44 13.032271696557416 7 45 13.908956916099768 7 46 16.26453733008354 7 47 15.667235075848524 7 48 17.311883844446875 7 49 17.876852862357065 7 50 16.01871021135727 7 51 13.964303297076409 7 52 13.9897671189688 7 53 15.45482405083245 7 54 15.55663841294093 7 55 9.964245395127751 7 56 12.700199170577317 8 1 4.641687280972988 8 2 5.677008682260777 8 3 6.741081887300368 8 4 6.763222561751974 8 5 6.767732850716037 8 6 6.157447524464324 8 7 5.001366000945829 8 8 5.928764232965911 8 9 6.128835776734924 8 10 6.7448476360241045 8 11 6.3244325002728345 8 12 6.688106151551526 8 13 9.310681848496973 8 14 7.420163519953434 8 15 9.60072422908556 8 16 8.884133350309817 8 17 11.152517067433045 8 18 8.522884002085682 8 19 7.633468842082291 8 20 9.186693768416454 8 21 6.893836322407754 8 22 6.762558659827572 8 23 7.110890719924324 8 24 7.7375450180072 8 25 6.691986491566318 8 26 7.732533013205277 8 27 5.706246134817569 8 28 3.6954024033855966 8 29 5.448659160633962 8 30 7.8236094437774995 8 31 6.725619641796115 8 32 6.2081159736621885 8 33 6.573302351243527 8 34 7.464072295584895 8 35 6.547843379776147 8 36 8.742725878230083 8 37 8.333768355827178 8 38 9.652969369565987 8 39 10.124074781427723 8 40 4.051315374634701 8 41 15.857534225811534 8 42 13.075409254610939 8 43 14.420151697042456 8 44 14.043382807668529 8 45 13.37562358276643 8 46 15.16453733008354 8 47 16.711679520292968 8 48 15.534106066669096 8 49 16.565741751245948 8 50 14.14093243357949 8 51 12.43096996374307 8 52 12.589767118968801 8 53 11.754824050832447 8 54 12.64552730182982 8 55 10.797578728461087 8 56 12.344643615021763 9 1 3.519465058750768 9 2 3.7547864600385523 9 3 5.841081887300369 9 4 5.285444783974192 9 5 4.8455106284938125 9 6 4.8685586355754396 9 7 4.012477112056942 9 8 4.139875344077026 9 9 3.9177246656238083 9 10 4.9892920804685446 9 11 4.0799880558283945 9 12 5.632550595995969 9 13 7.11068184849697 9 14 5.309052408842319 9 15 3.8673908957522265 9 16 8.273022239198703 9 17 6.796961511877491 9 18 9.70066177986346 9 19 9.100135508748952 9 20 7.631138212860897 9 21 6.4938363224077555 9 22 6.429225326494237 9 23 6.133112942146543 9 24 7.11532279578498 9 25 4.60309760267743 9 26 4.24364412431639 9 27 3.961801690373129 9 28 3.6287357367189337 9 29 5.893103605078405 9 30 5.9458316659997195 9 31 2.647841864018339 9 32 5.05256041810663 9 33 3.539969017910188 9 34 4.697405628918229 9 35 5.6922878242205925 9 36 7.142725878230081 9 37 6.422657244716067 9 38 6.652969369565987 9 39 7.546297003649947 9 40 -0.27090684758752204 9 41 9.89086755914487 9 42 9.530964810166495 9 43 10.309040585931342 9 44 10.432271696557414 9 45 10.608956916099764 9 46 12.364537330083543 9 47 15.267235075848525 9 48 15.611883844446872 9 49 15.876852862357065 9 50 15.418710211357268 9 51 15.064303297076403 9 52 14.789767118968804 9 53 15.854824050832448 9 54 11.456638412940936 9 55 15.064245395127752 9 56 16.200199170577317 10 1 3.1694650587507667 10 2 3.329786460038555 10 3 2.6160818873003677 10 4 1.860444783974195 10 5 4.170510628493815 10 6 5.143558635575438 10 7 4.487477112056943 10 8 5.664875344077025 10 9 5.26772466562381 10 10 6.064292080468547 10 11 6.629988055828392 10 12 5.132550595995969 10 13 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5.4089159906386755 116 21 7.116058544629976 116 22 7.651447548716458 116 23 7.44422405325766 116 24 7.9597672402294215 116 25 7.803097602677433 116 26 5.732533013205277 116 27 6.150690579262012 116 28 6.250957958941154 116 29 7.004214716189519 116 30 7.490276110444164 116 31 6.170064086240558 116 32 5.541449306995517 116 33 7.462191240132412 116 34 5.6862945178071165 116 35 6.103398935331704 116 36 9.076059211563418 116 37 9.5559905780494 116 38 9.986302702899323 116 39 10.457408114761058 116 40 12.82909315241248 116 41 12.413089781367091 116 42 6.853187032388718 116 43 6.642373919264674 116 44 4.821160585446304 116 45 11.042290249433101 116 46 2.831203996750208 116 47 -2.3994315908181427 116 48 -0.9103383777753464 116 49 3.899075084579284 116 50 5.252043544690601 116 51 8.875414408187517 116 52 8.034211563413244 116 53 8.088157384165779 116 54 6.756638412940934 116 55 7.2420231729055295 116 56 11.01131028168843 117 1 2.2527983920841024 117 2 3.788119793371891 117 3 4.074415220633703 117 4 4.485444783974195 117 5 4.378843961827151 117 6 4.101891968908774 117 7 4.445810445390279 117 8 3.8732086774103607 117 9 4.351057998957145 117 10 2.6892920804685474 117 11 1.3799880558283917 117 12 3.632550595995969 117 13 4.810681848496973 117 14 -1.8576142578243484 117 15 -8.899275770914443 117 16 -14.560311094134631 117 17 -6.903038488122512 117 18 0.4673284465301286 117 19 -2.533197824584377 117 20 -3.868861787139103 117 21 5.893836322407754 117 22 6.929225326494237 117 23 5.666446275479881 117 24 4.181989462451643 117 25 6.303097602677433 117 26 4.176977457649727 117 27 4.261801690373126 117 28 5.362069070052268 117 29 4.393103605078405 117 30 5.545831665999721 117 31 3.9478418640183364 117 32 3.8192270847732956 117 33 5.573302351243527 117 34 4.297405628918231 117 35 5.158954490887254 117 36 6.909392544896747 117 37 7.8893239113827285 117 38 8.486302702899323 117 39 9.17963033698328 117 40 11.32909315241248 117 41 8.19086755914487 117 42 1.4642981434998283 117 43 -3.02429274740199 117 44 -2.0677283034425855 117 45 7.7089569160997655 117 46 -3.0021293365831276 117 47 1.1005684091818573 117 48 -3.354782822219793 117 49 1.8435195290237303 117 50 0.5853768780239363 117 51 5.930969963743074 117 52 6.256433785635469 117 53 5.421490717499115 117 54 5.423305079607601 117 55 5.964245395127751 117 56 8.40019917057732 118 1 2.5444650587507667 118 2 3.579786460038555 118 3 4.241081887300368 118 4 4.235444783974195 118 5 3.6705106284938154 118 6 4.268558635575438 118 7 3.7374771120569434 118 8 4.039875344077025 118 9 4.01772466562381 118 10 4.064292080468547 118 11 1.5049880558283917 118 12 -9.24244940400403 118 13 -0.6893181515030271 118 14 0.18405240884231944 118 15 -12.232609104247775 118 16 -9.851977760801297 118 17 -0.40303848812251175 118 18 -3.4493382201365392 118 19 -1.324864491251045 118 20 -0.24386178713910311 118 21 5.268836322407754 118 22 6.304225326494237 118 23 5.0831129421465455 118 24 4.890322795784979 118 25 5.678097602677433 118 26 4.218644124316391 118 27 5.261801690373126 118 28 4.903735736718932 118 29 5.268103605078405 118 30 5.920831665999721 118 31 5.197841864018336 118 32 5.652560418106631 118 33 4.489969017910191 118 34 5.422405628918231 118 35 4.74228782422059 118 36 7.1177258782300825 118 37 7.347657244716064 118 38 7.652969369565987 118 39 9.471297003649944 118 40 10.20409315241248 118 41 8.06586755914487 118 42 7.255964810166496 118 43 5.934040585931342 118 44 7.0572716965574145 118 45 5.9589569160997655 118 46 -2.08546266991646 118 47 7.017235075848525 118 48 -2.313116155553125 118 49 1.5518528623570624 118 50 0.1687102113572685 118 51 0.7643032970764061 118 52 2.339767118968801 118 53 2.629824050832447 118 54 4.381638412940934 118 55 -1.535754604872249 118 56 2.77519917057732 119 1 2.5623222016079126 119 2 3.597643602895701 119 3 4.883939030157514 119 4 4.414016212545626 119 5 3.6883677713509613 119 6 4.554272921289723 119 7 4.398191397771228 119 8 3.6113039155055944 119 9 3.589153237052379 119 10 4.332149223325693 119 11 -0.977154801314466 119 12 -6.7245922611468885 119 13 -3.4036038657887424 119 14 -8.405233305443396 119 15 -5.01832338996206 119 16 -5.79840633222987 119 17 -0.40303848812251175 119 18 -6.556481077279397 119 19 1.300135508748955 119 20 0.059709641432323934 119 21 4.0366934652649 119 22 5.143511040779952 119 23 4.047398656432261 119 24 3.3724656529278327 119 25 3.9459547455345785 119 26 3.5579298386021065 119 27 4.261801690373126 119 28 4.028735736718932 119 29 5.17881789079269 119 30 5.760117380285436 119 31 4.090699006875482 119 32 0.0811318466780584 119 33 4.954254732195906 119 34 5.3688342003468 119 35 5.42085925279202 119 36 6.7427258782300825 119 37 5.651228673287495 119 38 7.795826512423133 119 39 9.203439860792798 119 40 11.543378866698195 119 41 5.619438987716297 119 42 2.916679095880781 119 43 -6.119530842640085 119 44 -7.353442589156872 119 45 4.99467120181405 119 46 3.021680187226398 119 47 -2.732764924151475 119 48 -6.545259012695983 119 49 -0.32314713764293757 119 50 2.3472816399286955 119 51 6.621446154219264 119 52 2.3040528332545165 119 53 -0.9594616634532684 119 54 -4.243361587059066 119 55 -0.9643260334436761 119 56 -3.528372257994107 120 1 2.8194650587507653 120 2 3.454786460038555 120 3 2.4410818873003706 120 4 3.3854447839741937 120 5 3.3455106284938125 120 6 3.468558635575441 120 7 3.1124771120569434 120 8 2.9398753440770236 120 9 3.0177246656238097 120 10 0.7892920804685488 120 11 -0.7200119441716097 120 12 -3.56744940400403 120 13 -1.8893181515030264 120 14 -7.490947591157681 120 15 -4.232609104247775 120 16 -7.326977760801299 120 17 -3.003038488122513 120 18 -2.99933822013654 120 19 -2.9998644912510457 120 20 -3.4688617871391045 120 21 -3.0061636775922445 120 22 -0.47077467350576185 120 23 -1.6668870578534545 120 24 -0.5846772042150228 120 25 -0.596902397322566 120 26 -2.1563558756836088 120 27 -1.3381983096268755 120 28 -2.071264263281069 120 29 -0.40689639492159557 120 30 -0.1541683340002784 120 31 -0.7521581359816629 120 32 -1.047439581893368 120 33 -1.8600309820898104 120 34 -1.9025943710817685 120 35 -0.6077121757794117 120 36 0.4427258782300818 120 37 0.6226572447160628 120 38 0.452969369565988 120 39 2.4462970036499456 120 40 4.529093152412479 120 41 -6.40913244085513 120 42 -3.869035189833504 120 43 -5.5909594140686565 120 44 -14.767728303442585 120 45 -4.891043083900236 120 46 -7.23546266991646 120 47 -9.032764924151476 120 48 -7.488116155553126 120 49 -13.723147137642938 120 50 -7.881289788642732 120 51 -5.035696702923595 120 52 -3.610232881031198 120 53 -3.4451759491675524 120 54 -3.2433615870590664 120 55 -3.535754604872249 120 56 -1.3998008294226807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 254.0 5 28.0 6 13.0 7 16.0 8 35.0 9 27.0 10 13.0 11 22.0 12 15.0 13 15.0 14 25.0 15 25.0 16 38.0 17 56.0 18 41.0 19 81.0 20 94.0 21 110.0 22 113.0 23 154.0 24 181.0 25 202.0 26 179.0 27 177.0 28 168.0 29 227.0 30 245.0 31 297.0 32 349.0 33 358.0 34 414.0 35 467.0 36 460.0 37 478.0 38 465.0 39 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.30115390866244 0.4063058670567203 0.16252234682268812 0.1300178774581505 8 98.94360474565252 0.7150983260198277 0.22753128555176336 0.11376564277588168 9 94.40923126929953 0.9426296115715912 3.624248334145945 1.023890784982935 10 16.723549488054605 11.604095563139932 61.88850967007964 9.783845278725826 11 9.735088574679018 20.039005363237443 6.988460913375589 63.23744514870795 12 19.128880221030393 59.28815212091663 3.981797497155859 17.601170160897123 13 7.232244433609622 16.626036079960997 5.850804485616772 70.29091500081262 14 11.149032992036405 68.53567365512758 6.760929627823825 13.55436372501219 15 64.17837308069258 13.55766089513231 9.57203528258739 12.691930741587717 16 8.994795055302538 62.41054001301236 18.37996096291477 10.214703968770332 17 12.662548764629388 11.524707412223666 62.46749024707412 13.345253576072821 18 13.099301153908662 7.979847228993988 10.190151145782544 68.7307004713148 19 7.321835340058574 7.891311422063131 18.51610803774813 66.27074520013016 20 8.950366151342555 13.03498779495525 64.13344182262001 13.881204231082181 21 8.174874045181213 8.954981309930115 17.81244921176662 65.05769543312205 22 8.792458963107427 11.067771818625062 65.10645213716886 15.033317081098652 23 11.961644726149846 16.317243620997886 61.23842028278889 10.482691370063383 24 6.664499349804942 20.04226267880364 13.589076723016905 59.70416124837451 25 12.221680481066146 15.618397529660328 59.84072810011376 12.31919388915976 26 65.96747967479675 17.72357723577236 9.414634146341463 6.89430894308943 27 16.79140904653433 66.4497233973316 7.061503416856492 9.697364139277578 28 38.53404843165935 49.26052332195677 6.43588493417845 5.769543312205428 29 41.133365901319 42.61520924930793 10.74743527112848 5.503989578244585 30 35.67479674796748 21.495934959349594 36.65040650406504 6.178861788617886 31 39.980497318381275 39.72046156346497 15.08207378514546 5.216967333008289 32 39.43089430894309 41.4959349593496 13.252032520325201 5.821138211382114 33 41.148714611129186 38.398958672307195 12.77253498210218 7.679791734461439 34 14.195018720494874 12.599706983558523 64.18687937489825 9.018394921048348 35 12.239583333333332 15.950520833333334 64.04622395833334 7.763671875 36 36.168482680110586 13.302976093673768 39.59993494877216 10.928606277443487 37 38.15725215692659 12.567149601172064 39.23164577567964 10.043952466221716 38 38.78847093307279 12.76665038267383 37.87656733431037 10.568311349943006 39 36.09438567941416 15.899104963384866 15.49227013832384 32.51423921887714 40 32.012444735549366 12.706729981987882 36.073358441133124 19.20746684132962 41 25.451559934318556 13.119868637110017 32.758620689655174 28.669950738916256 42 13.885267275097785 39.63494132985658 17.177314211212515 29.302477183833116 43 7.9973886078015335 39.04031336706382 33.866492573853435 19.095805451281215 44 10.871342161190126 39.39839790747099 36.619257806114106 13.111002125224783 45 31.72638436482085 19.087947882736156 35.78175895765472 13.403908794788274 46 9.126530612244899 42.00816326530612 14.808163265306124 34.05714285714286 47 9.71540726202159 36.7844291789336 11.007523716061499 42.492639842983316 48 31.923202082655383 22.40481614057924 13.407094044907256 32.264887731858124 49 8.68004568445097 39.25599608418992 14.667971936694405 37.39598629466471 50 7.572056668295065 45.562611952450744 13.727405959941377 33.137925419312815 51 9.369398729020695 44.74498940850579 13.817826299494868 32.067785562978656 52 9.55870379417033 65.65705911089398 15.209249307930303 9.574987787005373 53 8.106344805088893 61.865927254933936 16.750937856793346 13.27679008318382 54 8.32381263260976 25.60796474620532 34.69887383711441 31.369348784070507 55 10.516875917169411 19.827164519810857 40.290233164845915 29.365726398173813 56 10.430247718383312 22.42503259452412 36.29400260756193 30.850717079530636 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 5.0 10 3.5 11 2.0 12 6.5 13 18.5 14 26.0 15 21.0 16 16.0 17 11.0 18 6.0 19 13.0 20 20.0 21 15.5 22 21.5 23 32.0 24 37.5 25 43.0 26 44.0 27 45.0 28 50.5 29 62.5 30 69.0 31 61.0 32 53.0 33 48.5 34 44.0 35 46.5 36 49.0 37 64.0 38 67.0 39 55.0 40 66.5 41 78.0 42 91.5 43 105.0 44 128.0 45 151.0 46 293.0 47 746.5 48 1058.0 49 1227.5 50 1397.0 51 1043.0 52 689.0 53 540.0 54 354.0 55 317.0 56 291.0 57 265.0 58 228.0 59 191.0 60 177.0 61 163.0 62 136.0 63 87.0 64 65.0 65 56.5 66 48.0 67 39.0 68 30.0 69 21.5 70 13.0 71 11.5 72 9.5 73 9.0 74 6.0 75 3.0 76 1.5 77 0.0 78 0.5 79 1.0 80 1.0 81 1.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5038192751503331 16 0.08126117341134406 17 0.01625223468226881 18 0.0 19 0.11376564277588168 20 0.1300178774581505 21 0.0 22 0.0 23 0.0 24 0.01625223468226881 25 0.0 26 0.04875670404680643 27 0.11376564277588168 28 0.0 29 0.19502681618722573 30 0.04875670404680643 31 0.0 32 0.04875670404680643 33 0.11376564277588168 34 0.16252234682268812 35 0.14627011214041932 36 0.06500893872907525 37 0.16252234682268812 38 0.19502681618722573 39 0.1300178774581505 40 0.7476027953843654 41 1.023890784982935 42 0.2762879895985698 43 0.42255810173898906 44 0.5850804485616773 45 0.21127905086949453 46 0.4550625711035267 47 0.6338371526084837 48 0.11376564277588168 49 0.39005363237445145 50 0.19502681618722573 51 0.260035754916301 52 0.19502681618722573 53 0.35754916300991385 54 0.42255810173898906 55 0.32504469364537625 56 0.2762879895985698 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 6153.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.09783845278726 #Duplication Level Percentage of deduplicated Percentage of total 1 90.53356282271945 59.84072810011376 2 4.917629702483403 6.5008938729075245 3 1.549053356282272 3.0716723549488054 4 1.0081140890090976 2.6653664878920855 5 0.4671748217359233 1.5439622948155372 6 0.27046963363658716 1.0726474890297415 7 0.29505778214900413 1.3651877133105803 8 0.14752889107450207 0.7801072647489029 9 0.09835259404966806 0.5850804485616773 >10 0.5901155642980083 6.598407281001138 >50 0.04917629702483403 2.5516008451162033 >100 0.07376444553725103 13.42434584755404 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 346 5.623273200065009 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 346 5.623273200065009 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 134 2.1777994474240208 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 97 1.5764667641800747 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 60 0.9751340809361287 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 41 0.6663416219730214 No Hit AACGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 36 0.5850804485616773 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 33 0.5363237445148707 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 22 0.35754916300991385 No Hit AACGCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 20 0.32504469364537625 No Hit AACGCCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT 19 0.3087924589631074 No Hit AACGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG 17 0.2762879895985698 No Hit AACGCCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 16 0.260035754916301 No Hit AACGCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC 15 0.24378352023403219 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT 15 0.24378352023403219 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA 15 0.24378352023403219 No Hit AACGCCGGGCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA 14 0.22753128555176336 No Hit AACGCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 14 0.22753128555176336 No Hit AACGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 14 0.22753128555176336 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTGTTCAATCCGCCCAATC 13 0.21127905086949453 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT 13 0.21127905086949453 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA 13 0.21127905086949453 No Hit AACGCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACT 12 0.19502681618722573 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCCATTGAACAAAAACTC 12 0.19502681618722573 No Hit AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG 11 0.17877458150495693 No Hit AACGCCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA 11 0.17877458150495693 No Hit AACGCCGGGTCCCCGCTCCTCTTCTGAAGGAGATTGGGCGGACTGCCCAAAAACTC 10 0.16252234682268812 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCCATCCGCCCAATCT 10 0.16252234682268812 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT 10 0.16252234682268812 Illumina Single End Adapter 2 (95% over 24bp) AACGCCGGGGTGCAGTGCTAACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGG 9 0.14627011214041932 No Hit AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 9 0.14627011214041932 No Hit AACGCCGGGTCACAGAACCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 9 0.14627011214041932 No Hit AACGCCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 9 0.14627011214041932 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAAAAA 8 0.1300178774581505 No Hit AACGCCGGGTCACAGGTCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 8 0.1300178774581505 No Hit AACGCCGGGTCACCCATCCTCTTCTGAAGGAGATTGGGCGGATTGACACAAAACTC 8 0.1300178774581505 No Hit AACGCCGGGTCACAGAACCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 8 0.1300178774581505 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT 8 0.1300178774581505 Illumina Single End Adapter 1 (95% over 23bp) AACGCCGGGTCACAGATCCTCTTAAGGAGATTGGGCGGATTGAACAAAAACTCATC 8 0.1300178774581505 No Hit AACGCCGGGTCACCCATCCTCTTCTGAGATGAGTTTTTGTTCACTCCGCCCAATCT 7 0.11376564277588168 No Hit AACGCCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.11376564277588168 No Hit AACGCCGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 7 0.11376564277588168 No Hit AACGCCGGGTCACAGACCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 7 0.11376564277588168 No Hit AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTGTTCAATCCGCCCAATCTC 7 0.11376564277588168 No Hit AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.11376564277588168 No Hit AACGCCGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 7 0.11376564277588168 No Hit AACGCCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA 7 0.11376564277588168 No Hit AACGCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATCGAACAAAAACTCATC 7 0.11376564277588168 No Hit AACGCCGGGTCCCCCCTCCTCTTCTGAAGGAGATTGGGCGGATCGACACAAAACTC 7 0.11376564277588168 No Hit AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTT 7 0.11376564277588168 No Hit AACGCCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 7 0.11376564277588168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.01625223468226881 0.0 0.0 0.0 0.0 13 0.03250446936453762 0.0 0.0 0.0 0.0 14 0.16252234682268812 0.0 0.0 0.0 0.0 15 0.16252234682268812 0.0 0.0 0.0 0.0 16 0.16252234682268812 0.0 0.0 0.0 0.0 17 0.16252234682268812 0.0 0.0 0.0 0.0 18 0.16252234682268812 0.0 0.0 0.0 0.0 19 0.16252234682268812 0.0 0.0 0.0 0.0 20 0.16252234682268812 0.0 0.0 0.0 0.0 21 0.16252234682268812 0.0 0.0 0.0 0.0 22 0.16252234682268812 0.0 0.0 0.0 0.0 23 0.16252234682268812 0.0 0.0 0.0 0.0 24 0.16252234682268812 0.0 0.0 0.0 0.0 25 0.16252234682268812 0.0 0.0 0.0 0.0 26 0.16252234682268812 0.0 0.0 0.0 0.0 27 0.21127905086949456 0.0 0.0 0.0 0.0 28 0.21127905086949456 0.0 0.0 0.0 0.0 29 0.22753128555176336 0.0 0.0 0.0 0.0 30 0.22753128555176336 0.0 0.0 0.0 0.0 31 0.22753128555176336 0.0 0.0 0.0 0.0 32 0.22753128555176336 0.0 0.0 0.0 0.0 33 0.22753128555176336 0.0 0.0 0.0 0.0 34 0.22753128555176336 0.0 0.0 0.0 0.0 35 0.24378352023403219 0.0 0.0 0.0 0.0 36 0.24378352023403219 0.0 0.0 0.0 0.0 37 0.24378352023403219 0.0 0.0 0.0 0.0 38 0.24378352023403219 0.0 0.0 0.0 0.0 39 0.24378352023403219 0.0 0.0 0.0 0.0 40 0.24378352023403219 0.0 0.0 0.0 0.0 41 0.260035754916301 0.0 0.0 0.0 0.0 42 0.3087924589631074 0.0 0.0 0.0 0.0 43 0.32504469364537625 0.0 0.0 0.0 0.0 44 0.35754916300991385 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTCC 20 3.794708E-4 50.18033 37 AATCCTC 20 3.794708E-4 50.18033 15 AAATCCT 20 3.794708E-4 50.18033 14 CTCCTCT 25 1.8123364E-5 50.18033 16 GGCCGAT 15 0.007923533 50.18033 37 TTTTTGC 15 0.007923533 50.18033 34 TTGGGCC 15 0.007923533 50.18033 34 CCATCCG 15 0.007923533 50.18033 42 TCCATCC 15 0.007923533 50.18033 41 TGGGCCG 15 0.007923533 50.18033 35 GCTCCTC 20 3.794708E-4 50.18033 15 GCCGATT 15 0.007923533 50.18033 38 CCGATTG 15 0.007923533 50.18033 39 TTTGCTC 15 0.007923533 50.18033 36 CGATTGA 15 0.007923533 50.18033 40 GGGCCGA 15 0.007923533 50.18033 36 GTCACAA 20 3.9533508E-4 49.77236 9 CGGGGAG 20 3.9533508E-4 49.77236 6 GCCGGGC 40 2.0954758E-9 49.77236 4 CCGGGGA 20 3.9533508E-4 49.77236 5 >>END_MODULE