FastQCFastQC Report
Fri 17 Jun 2016
SRR1525965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8553
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGATGAGATGGACTTAAAGGAATTGACGGGTAC440.5144393779960248No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTAC410.47936396585993213No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC360.4209049456331112No Hit
AACGCCGGGATGAGATGGCCTTAAATGAATTGACGGGTAC300.350754121360926No Hit
AACGCCGGGATGAGATGGACTCAAAGGAATTGACGGGTAC220.2572196889980124No Hit
AACGCCGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC210.2455278849526482No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG190.2221442768619198No Hit
AACGCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC160.18706886472582718No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG160.18706886472582718No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC160.18706886472582718No Hit
AACGCCGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC150.175377060680463No Hit
AACGCCGGGATGAGATGGACTCAAATGAATTGACGGGTAC140.16368525663509878No Hit
AACGCCGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC140.16368525663509878No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG130.1519934525897346No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC110.1286098444990062No Hit
AACGCCGGGATGAGATGGCCTCAAATGAATTGACGGGTAC110.1286098444990062No Hit
AACGCCGGGACCTTTATGTTACTTTATGCTGCTGGCTCTT100.11691804045364199No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG100.11691804045364199No Hit
AACGCCGGGATGCCACAACTAGATACATCAACATGATTTA100.11691804045364199No Hit
AACGCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT100.11691804045364199No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACCGGTAC90.1052262364082778No Hit
AACGCCGGGGGGCTGGTGCGATGGCTCAGTGGGTTAGAGC90.1052262364082778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATT301.2549555E-534.24260725
TGAATTG301.2549555E-534.24260726
AATGAAT301.2549555E-534.24260724
TAAATGA251.7871303E-434.24260322
CTTAAAT251.7871303E-434.24260320
TTAAATG251.7871303E-434.24260321
CGATGGC200.002545622234.242619
GATGGCC200.002621255834.04117614
GCGATGG200.002621255834.04117618
ATGGCCT200.002621255834.04117615
CGGGGGG1800.033.8421066
CGGGGAG301.3622457E-533.8421066
CGGGCTG301.3622457E-533.8421066
CGGGTGG301.3622457E-533.8421066
CGGGCAG200.00269865833.8421066
GGTTAAG200.00269865833.84210632
GCCGGGC850.033.8421064
CGGGATT200.00269865833.8421066
CGGGATG552.3646862E-1133.8421066
CGGGATC301.3622457E-533.8421066
Produced by FastQC (version 0.11.5)