FastQCFastQC Report
Fri 17 Jun 2016
SRR1525964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTT21.25No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTGACGGGTAC21.25No Hit
AACGCCGGTACGTCGTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGACTTAACTGAATTGCCGGGCCC10.625No Hit
AACGCCGGGAACAGTGGTTCAACGCCAAAAAAAAAAAAAA10.625No Hit
AACGCCCGGCAGACTGGTTAACCCCCCGCCGCCCTTTTTT10.625No Hit
AACGCCGGGTGCCCCAGCCTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAACAGTATCCCCCCCAGTCGCCTTTTTTTTT10.625No Hit
AACGCCGGGCTGGACAGCGACTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGACCAGTAAAACCACCCCAAAGCCCACTGCTA10.625No Hit
AACGCCGGGCCTGTATCACGAGAGCCGTTTTTTTTTTTTT10.625No Hit
AACGCCGGGGATGAGATGGGAAAACCACAAACCCCCCCCC10.625No Hit
AACGCCGGGTGGTTAGACGCCTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAGCTTCCAACCCCCAGCCCCCCTTTTTTCTT10.625No Hit
AACGCCGGGTGGACCACCCCGAGCCGCCTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGCCCCTAAATGAATTGCCGGGTAC10.625No Hit
AACGCCGGGACCATACCACGCAGCGCCGACTCTTTTTTTT10.625No Hit
AACGCCGGGCAGTGGTTACCCCGCGCCGCCTTTTTTTGAC10.625No Hit
AACGCCGGGAGCTATCAACCCAAAAAAAAAAAAAAAAAAA10.625No Hit
AACGCCGGGCCCCTTTTTTTTTTTTCCTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGGTTCCAGCAGAGCCTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGGGGCAGTGCAAACCCCCGCCCCCCCCATCCC10.625No Hit
AACGCCGGGAACAACCAACAAAAAAAAAAAAAAAAAAAAA10.625No Hit
AACGCCGGGGCCCTTAACCCAGAGTCCATTTTCTTTTTTT10.625No Hit
AACGCCGGGTCCCAGCCCTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGCTGAGATGGCCCCAAAGGAATTAACGGGCAC10.625No Hit
AACGCCGGGATGAGCTGGCCTCAAAGGAATTGCCGGGTAA10.625No Hit
AACGCCGGGCAGCCGTGGTCTCCCCCCCGGCCTTTCTTTA10.625No Hit
AACGCCGGGTGACGCAGAGCCCCTTTTTTTTCTTTTTTTC10.625No Hit
AACGCCGGGAAGATGGTATCACCCCCAGCCGACTTTTTTT10.625No Hit
AACGCCGGGGGAGAAAGAAATGGCTCCCCCGGCCCCCCCT10.625No Hit
AACGCCGGGCCACATGCCAACGCACACTCGACTTTTTTTT10.625No Hit
AACGCCGGGAACCATGGCATCCCCCCCAAACCCCACCCCC10.625No Hit
AACGCCGGGAACCAGTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGCCGAGCTGGCCACAAAAAAAAAACCCCAAAA10.625No Hit
AACGCCGGGATGGAACGCCGAGCCGCCTTTTTTTCTTTTT10.625No Hit
AACGCCGGGGGTGTACCACCCAGAGTCGCCTTTTTTTTTT10.625No Hit
AACGCCGGGTGCTGAGAAAGACCTTTCCGGCATTTCTCCT10.625No Hit
AACGCCNGGGCAATTCGTGGAAAACGAAATCCCCCCCCTC10.625No Hit
AACGCCGGGAGTGGATCACCCCCGAGCCGACCCCCGTTGC10.625No Hit
AACGCCGGGCCTGGTCTCAACCCAAAAAAAACACACACAA10.625No Hit
AACGCCGGGAAGCAGTGGTACGCAGACCGCCTTTTTTTTT10.625No Hit
AACGCCGGGCCCGCCTTTTTTCTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAAGCTTATCAACCCACAGCCGACTTTTTTTT10.625No Hit
AACGCCGGGATCCCGTCGATTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAGTGCCACGCAGCGTCCACTTTTTTTTTTTT10.625No Hit
AACGCCGAGGAAAAAAAACAAAAACAAAAAAAACAAAAAA10.625No Hit
AACGCCCGGACTGTACACCCCCCCCCCCCCCCTCTTTTTC10.625No Hit
AACGCCGGGGTGGCCACCCCGAGTCCACTTTTTTTTTTTT10.625No Hit
AACGCCGGGCCCTCGTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGTGGTCGAGATCGATTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGCCGAGTCGACTTTTTTTTTTTTCCTTTTTTT10.625No Hit
AACGCCGGGCCGAGATGGACTCCCCTGCCTTGCCGGGTCC10.625No Hit
AACGCCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGAACCGTGGTATCAACGCAGCGCCCCTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGGGAATTGACGGTGAGTGGGTCT10.625No Hit
AACGCCGAGTGGACGCACGTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGTGCGCTTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGACAGTCTCAACCCCGATCGACTTTTTATTTT10.625No Hit
AACGCCGAATCCCTCCCCCCGTGCATCGCCCTGGGGGTCG10.625No Hit
AACGCCGGGCAGCATGTTCAACCCAGAGCCGACCTTTTTT10.625No Hit
AACGCCGGGAAGCATGGACAACCCAGGGCCCACCCTTTTT10.625No Hit
AACGCCATCTCATCCCGGCGTTCGCCCTGTTCTGCGTCGG10.625No Hit
AACGCCGGGCAACAGAACTATCACCTCTCCCCTCCCTCCT10.625No Hit
AACGCCGGGAACATGGTATCAACGCCCCACACCCCCACCC10.625No Hit
AACGCCGGGCTGAGCTGGAAAGGAATTGACGGGTACGCAC10.625No Hit
AACGCCGGGACCGTTTCTCCACCCCGCGCCCCCTTTTTTT10.625No Hit
AACGCCGGGAACTTGCCCCACGCACACTCGCCTTTTATTT10.625No Hit
AACGCCGGGCCCACCGCTCCCCCCCGCCTTTCCCTCCGTC10.625No Hit
AACGCCGGGCCCCCGTGGTCACAGAGCCGATTTTTTTTTT10.625No Hit
AACGCCGGGAAGCATGCTACAACGCAGAGCCGCCTCCTTT10.625No Hit
AACGCCGGGATGAGCTGGGGAATTGACGGTGAGTGTGTCT10.625No Hit
AACGCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTNTTT10.625No Hit
AACGCCGGGGGCTCCGTGGTTCCGAGCCCCGCCTCCTCTT10.625No Hit
AACGCCGGGCAGTGTCCGCAGAGCCGCCTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGGCTTAAAGGAATTGACAGGTAC10.625No Hit
AACGCCGGGCTGAGATGGCCTTAAAGGAATTGACGGCTAC10.625No Hit
AACGCCNGGACGAGTATCACCGCAGAGCCGACTTTTTTTT10.625No Hit
AACGCCGGGAAGCTGGTCACCCAGAGTCGCCTTTTTTTTT10.625No Hit
AACGCCGGGTCCTTTCCGCTCCCCCATCGTGGGTGGAGCC10.625No Hit
AACGCCGGGCTGAGACTTAACCGAATTCACGGGTCCCCCC10.625No Hit
AACGCCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA10.625No Hit
AACGCCGGGATGAGATGGACTCAACTGACTTGCCGGGTCC10.625No Hit
AACGCCGGGATCCAGTGACAGATACCATTAGATTCAAGAG10.625No Hit
AACGCCGGGATGAGATGGACTCCACGNCNTTGCCGGGTCC10.625No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTCACGGGCAC10.625No Hit
AACGCCGGGTCCAGTGCTCACCCCCGCCCCCCGCCCCCAC10.625No Hit
AACGCCGGGCTGGTGTTTCGTGTACCGTTATTCCGACCAT10.625No Hit
AACGCCGGGACTGGTCACGCAGCGCCGCCTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGAATAAAATGAATTGACGGCTAC10.625No Hit
AACGCCGGGATGAGATGGCACTTAAATGAATTGACGGCTA10.625No Hit
AACGCCGAGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGCTGCCTATTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGACCATGGAACAACCCAGCCCCACCTCTCTTT10.625No Hit
AACGCCGGGATGAGATGGACTTAAATGAATTCACGGCTCC10.625No Hit
AACGCCGGGTCCAGAGCGTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGCCTTAAATGAATTGACGGGTCC10.625No Hit
AACGCCGGGCCGCACCATCCTTTGATACAAAACAAAAAAA10.625No Hit
AACGCCGGGCAAGAGCGATTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGCCGCATGGCATCACCCCAACAAACCCCAACC10.625No Hit
AACGCCGGGCGTCCACACAGCCCAAAACACCAAAACCCCA10.625No Hit
AACGCCGGGAAGTGACACCCAGATCCCCTTTTTTTTTTTT10.625No Hit
AACGCCGGGTAACCCCCACGTCCCCCTTTTTTTTTTCTTT10.625No Hit
AACGCCGGGAACAGAGCGACTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGCCTTAAATGACTTGACGGGCAC10.625No Hit
AACGCCGGCCGCAGTGATATCACCCCCACCAACCACCCCC10.625No Hit
AACGCCGGGAAGCTGGTCCACCCCCCGCCCCCCTTTTTTT10.625No Hit
AACGCCGGGACGAGCAACCGAGCCCACTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAAACGAACCACAAAACCCAAAACCAAAAACA10.625No Hit
AACGCCGGGATGAGATGGGGCAACCCACAGTCCTAGTCCG10.625No Hit
AACGCCGGGAAGCATGGTTCAACGCAGATCCACTTTTTTT10.625No Hit
AACGCCGGGACACTATAACCCAGAGTCGACTCTTTTTTTT10.625No Hit
AACGCCGGGACGCAGTTTCCACCCCGTCGCCTTTTTTTTT10.625No Hit
AACGCCGGGAAGCATGGTATCAACCAGAGCCGACCTTTTT10.625No Hit
AACGCCGGGGACGCCACCAGAGTCGACTTTTTTTTTTTTT10.625No Hit
AACGCCGGGGACTGGTATCCCCCGGGTCGCCTTTTTTTTT10.625No Hit
AACGCCGGGCCGCAGTCCCCAGAGTGCAGTGCTAACGCCG10.625No Hit
AACGCCGGGATGAGCTGGCCTTAAAGGAATTGCCGGGTCC10.625No Hit
AACGCCGGGAAGCAGTGTATCAACCCAGAGCTTTTTTTTT10.625No Hit
AACGCCGGGCCCTACTCAACCCCAANCNNACCACCACACC10.625No Hit
AACGCCGGGTACGAAGTCCACCTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAAGCATGTACACCCCGCGCCGACTTTTTTTT10.625No Hit
AACGCCCACACAAACAAAACAAAACAAAAAAAATTAATAA10.625No Hit
AACGCCGGGCCGTGTACCAACCCAAAAAAAAAAAAAAAAA10.625No Hit
AACGCCGGGCTGAGCTGGCCTTAAATGAATTGCCGGGTAC10.625No Hit
AACGCCGGGAGATGGACGCAGAGTCGCCTTTTTTTTTTTT10.625No Hit
AACGCCGGGGGGAATTCGTGGAGAAAGAAATGCCTCCCCC10.625No Hit
AACGCCGGGGAGTCGCCTTTTTTTTTCTTTTCTTTTTTTT10.625No Hit
AACGCCGGGAAGAGTCGACAACCAGCGCCGCCTTTTTTTT10.625No Hit
AACGCCGGGCTGAGCTGGGCTCAAATGAATTGACGGGTAC10.625No Hit
AACGCCGGGCTGGCAGAGTCGACTTTTCTTTTTTTTTTTT10.625No Hit
AACGCCGGGAACTGGCCCCGCAGAGCCGCCTCTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGGGATGATAGATTCACCCAGATT10.625No Hit
AACGCCACCAGATCTCCCCCCCCCCCTCCCCCTCCCTTCT10.625No Hit
AACGCCGGGGCCTTTTTTTTTTTGCCTTTTTTTTTTTTTC10.625No Hit
AACGCCGGGAAACAAACCCCAACACCCCCCCCCGCTTCGC10.625No Hit
AACGCCNGGACGCATGGTATAACCCCGAGTCGACTTTTTT10.625No Hit
AACGCCGAGACTGTCCACCCGAGCCCCTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGCCTTAAATGAATCCACGGGTAC10.625No Hit
AACGCCGGGATGAGCTGCACTTAAATGACTTGCCGGGTAC10.625No Hit
AACGCCGGGATGAGCTGGACTTAAAGGAATTGACGTGTAC10.625No Hit
AACGCCGGGTACAGCGCGTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGATGAGATGGACACTGGAGAGGCCTGCACCGC10.625No Hit
AACGCCGGGAAGCATGGTTACCCAAAAAAAAAAAAAAAAC10.625No Hit
AACGCCGGCCACGCCTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGAAGCATGGTCTCAACCCAAAACCAAAAAACA10.625No Hit
AACGCCGGGTACGTCTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCCCCTCCCCACCCCCCCCCCCCCCCCCCTCTTCGA10.625No Hit
AACGCCGGGAATGGTAACCCCCGCCGCCTTTTTTTTTTTT10.625No Hit
AACGCCGGGACGCCGTGTCTCCCCCCCGCGTTTTTTTTTT10.625No Hit
AACGCCGGGAAGCATGGACCACCCAGAGTCGCCCCTTTTT10.625No Hit
AACGCCGGGGCTCGCCTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
AACGCCGGGACTTTTTTTTTTTTTCCTTTTTTCACCCACA10.625No Hit
AACGCCGGGAGTGGTTAAGAGAGTCCCCCTTTTTTTTTTT10.625No Hit
AACGCCGGGACCAGTGGTCCACCCCGCGTCGCCTTTTTTT10.625No Hit
AACGCCGGGACATGTATCAACGCCGCGCCGCCTTTTTTTT10.625No Hit
AACGCCGGGATGTTCGCAGAGTTTTTATCATGGATAACTG10.625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG150.034.01
ACGCCGG150.034.02
CGCCGGG150.034.03