FastQCFastQC Report
Fri 17 Jun 2016
SRR1525963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT2511.26126126126126No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG135.8558558558558556No Hit
AACGCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT52.2522522522522523No Hit
AACGCCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT41.8018018018018018No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31.3513513513513513No Hit
AACGCCGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT20.9009009009009009No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA20.9009009009009009No Hit
AACGCCGGGATCCTCTTCTTTTCCGGCTGGAACCATGGAG20.9009009009009009No Hit
AACGCCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT20.9009009009009009No Hit
AACGCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC20.9009009009009009No Hit
AACGCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT20.9009009009009009No Hit
AACGCCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG20.9009009009009009No Hit
AACGCCGGTCACAGATACTCTTCTGATATGAGTTTTTGTT10.45045045045045046No Hit
AACGCCGGGGAGGAGAGTTTAAGCGTGGTATCAAAAAAAA10.45045045045045046No Hit
AACGCCGGGGGACAAAAACACACACTACTCGCCATACATC10.45045045045045046No Hit
AACGCCGGGGAGTGCAGTGCTAACGCCGGGACGCCGGGTC10.45045045045045046No Hit
AACGCCGGGTCAAAGATCCTGATCGGAAGATATCGTATGC10.45045045045045046No Hit
AACGCCGGGGGCAGTGCTAACGCCGGGTGCTAACGCCGGC10.45045045045045046No Hit
AACGCCGGGTCAACGATCATCTTCTGAAGAATATTGGAAG10.45045045045045046No Hit
AACGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.45045045045045046No Hit
AACGCCGGGAAGGAAGGGTAACAACCTAGAACGCAGTTCT10.45045045045045046No Hit
AACGCCGGGTCACAAATCCTCTTCTGAAGGAGATAAGACA10.45045045045045046No Hit
AACGCCGGGTAAAAAAAACAAAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGCAGAGTGCAGTGCTAACGCCGGGTCACAGAT10.45045045045045046No Hit
AACGCCGGGTCACAAATTCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGTCACAGATAATCTTCTGATATGAGGATAGTA10.45045045045045046No Hit
AACGCCGGGCGTGATCCAGTGAGCATCCGCGTCATCGGAG10.45045045045045046No Hit
AACGCCGGGGGTCTCCGGCTGTAAGGTTGAATATTGAGTG10.45045045045045046No Hit
AACGCCGGGGAAGTGGTATAAACGCAGAGCTAACGCCGGG10.45045045045045046No Hit
AACGCCGGGAAGTAAAGGGTCCTGGGAGGGGCCTTGAGCA10.45045045045045046No Hit
AACGCCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCT10.45045045045045046No Hit
AACGCCGGGTCACAGATCAACTATAACCATGGCTATGGAG10.45045045045045046No Hit
AACGCCGGTCACAGATCCCCTTCTGAAGGAGATTGGGCGC10.45045045045045046No Hit
AACGCCGGGCGTGCAGTGCTAACGCGGGTCACATATCATC10.45045045045045046No Hit
AACGCCGGGACGCAGAGTGCAGTAGATTTCTGTGAGTTTG10.45045045045045046No Hit
AACGCCGGGGAGGTAGTGAAGAAAAATAACAATACAGGAC10.45045045045045046No Hit
AACGCCGGCAGCAGTGGTATCAACAAAAAAAGGGTAGAAT10.45045045045045046No Hit
AACGCCGGGTGTAAATATATAAAAATTACACCTTGCACCC10.45045045045045046No Hit
AACGCCGGGTCACAAATACTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGTCACATATACTCAAGGAGATTGGGCGGATTG10.45045045045045046No Hit
AACGCCGGGAGTGCTAACGCCGGGTCACAGATCCTCTTCT10.45045045045045046No Hit
AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTC10.45045045045045046No Hit
AACGCCGGGGTGCTAACCCGGGTCACAGATCTTCTTCTGA10.45045045045045046No Hit
AACGCCGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGCGTGGGGTCCCTCAGATTGAGGTTACTTTTG10.45045045045045046No Hit
AACGCCGGGCCACTGATCCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGCTTCCTCCCAAAAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGCACCAAACGGGGAAGAATGGGAAGAGAAATT10.45045045045045046No Hit
AACGCCGGGAAGCAGTGGAATCAACGCAGATAGAAAAAAA10.45045045045045046No Hit
AACGCCTAAAAAAAATAAATACATCTTTATTTATATTATT10.45045045045045046No Hit
AACGCCGGGTAAACGATCCTCTGAAGGAGATTGGGCGGAT10.45045045045045046No Hit
AACGCCGGGTCACAAATAATCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGTTTCTCCTCCTTAACAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGGGGAATTAGTGGAGAAAGAAATGGCTCCCAT10.45045045045045046No Hit
AACGCCGGGTCCAGATCTTCTTCTGAAGGAGATTGGGCGG10.45045045045045046No Hit
AACGCCGGGTCACCGCTCCTCTTCTGAGATGAGTTTTTTG10.45045045045045046No Hit
AACGCCGGGTCACAGATCATCTTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGGTCGCAGCTCCTCTTCTGAAGGAGAAGGAAA10.45045045045045046No Hit
AACGCCGGGAAGCAGTGAGATGGCTTAGTGGGTAAGAGCA10.45045045045045046No Hit
AACGCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGATCACAGATCCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGTCACAGATCACCTTCTGAGATGAGTTTTTGTT10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTG10.45045045045045046No Hit
AACGCCGGGCAACAGTGGTATCAACGCAGAGTCGACTTTT10.45045045045045046No Hit
AACGCCGGGTCAAAGATCCTCTTCCGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGAAGAAGTGGTATCAACGCAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGTCAACGATCCTCTTCTGAGATGAGTTTATGT10.45045045045045046No Hit
AACGCCGGGTCAAAGATCCTATTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGCAGATCCTCTTCTGAGATGAGTCTTTGTTCA10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTTT10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGACGCCGGGATCGAAATAGCCAAACCGATGTT10.45045045045045046No Hit
AACGCCGGGAGTGCAGTGATATACGCCGGGTTACAGATCC10.45045045045045046No Hit
AACGCCGGGCTTCTCCTCCTCAAAAAAAAAAAAAACAAAA10.45045045045045046No Hit
AACGCCGGGTAAAAGTTTGCACAACCATCATTCACTACAC10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTGGATGTGTTGGAAAAACT10.45045045045045046No Hit
AACGCCAGGTAACAGATCATCATCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGTCAAAAATCTTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGGATAGCAAATCTTTGGACCTAGACTAGAGGC10.45045045045045046No Hit
AACGCCGGGTCACAGATCCCCTTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGTCAAGGATCCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGTCACAGAACCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGCGTCAAAAATAATCTTCTGAGATGAGTTTTGTT10.45045045045045046No Hit
AACGCCGGGTCAAACATCATCTTCTGAAGAAGATTGGACG10.45045045045045046No Hit
AACGCCGGGAAGCAGTGAGATGGATTAGTGGGTAAGAGCA10.45045045045045046No Hit
AACGCCGGGAAGCAGTGGTATCAACGAAGAGTGCATTGCT10.45045045045045046No Hit
AACGCCGGGGAGGAGAACCCCGGTTTACGCTGATGACCGA10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCGTTAAAGGATTTAAAGTGG10.45045045045045046No Hit
AACGCCGGGAGCAATGGTATCAACTCAGAGTCGACTTTTT10.45045045045045046No Hit
AACGCCGGGGAAAAAAAACAAAAAAAAAAAAAAAATACTG10.45045045045045046No Hit
AACGCCGGGTCACAGATGATCGGCAGAGCTCGTATGCCGT10.45045045045045046Illumina Single End Adapter 1 (95% over 23bp)
AACGCCGGGAAGAAATGGTATTAACAAAAAATAAATTGTT10.45045045045045046No Hit
AACGCCGGGGGGGGGGGCTGGAGAGATGGCTTAGCGGTTA10.45045045045045046No Hit
AACGCCGGGGTGCAGTGCTAACGCCGGGTCACAGATCCTC10.45045045045045046No Hit
AACGCCGGGGGAAAAAAAAAAAAAAAAAAAACAAATTATC10.45045045045045046No Hit
AACGCCGGTTCACAGATCCTCTTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGTCACATGTACTCTTAAGGAGATTGGGCGGAT10.45045045045045046No Hit
AACGCCGGGACACAGATCCTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGCCGCTACTCAGACTACCCAGATGCTTACACC10.45045045045045046No Hit
AACGCCGGGACGTGGAACGCAGAGTCGACTTTTTTTTTTT10.45045045045045046No Hit
AACGCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACTC10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT10.45045045045045046No Hit
AACGCCGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGGGAATTCGTCGAGAAAGAAATGGCTCGTCTG10.45045045045045046No Hit
AACGCCGGGAAGCAGTGGTATAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGTCACAGAAACTCTTCTGAGATGAGTCTTTGT10.45045045045045046No Hit
AACGCCGGGCCCAAGGAGGAAACAGTTAGTGATCCTGAAG10.45045045045045046No Hit
AACGCCGGGAAACAAAAACAAAAAAAAAAAAAAAAATGAT10.45045045045045046No Hit
AACGCCGGGTCAAAGATCCTCTTCTTTTTATTAACCCATA10.45045045045045046No Hit
AACGCCGGGAAAAAAAAAAACAAAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGTGAGTGCAGTGCTATCGCCGGGTCACAGATCC10.45045045045045046No Hit
AACGCCGGGGGGCATGGGAGACAGAGCAAGACTCTGTCTC10.45045045045045046No Hit
AACGCCGGGGGAGTCGAGACAACCAAGAACAGCCAGGCCT10.45045045045045046No Hit
AACGCCGGGGGGAATTACTTCGATTCTGTTTTGCTACCCG10.45045045045045046No Hit
AACGCCGGGGAAGCAGTGGTATCAAATCAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGCGCAAAGTGCAGTGCTAACGCCGGGTCACAG10.45045045045045046No Hit
AACGCCGGGGCAGTTGTACCACGCAGAGTCAATTTTTTTT10.45045045045045046No Hit
AACGCCGGGCTCTTTATTTTTTTATGAAAAGGGAGAAGAG10.45045045045045046No Hit
AACGCCGGGGTTTTTGTTTCACAGATCCTCTTCTGAGATG10.45045045045045046No Hit
AACGCCGGGTCACAGATCTTCTTCTGAGATGAGTTTTTCT10.45045045045045046No Hit
AACGCCGAGTCACAGAGAAAAAGTTTGGAGGTGAGACAGA10.45045045045045046No Hit
AACGCCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGAG10.45045045045045046No Hit
AACGCCGGGGGGTTGTCTCTCCCTAAGCACACTCCTCCTC10.45045045045045046No Hit
AACGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA10.45045045045045046No Hit
AACGCCGGGTCAAAGCTCCTCTTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGGTCAAAAATCCTCTGAAGGAGATTGGGCGGA10.45045045045045046No Hit
AACGCCGGGTACGATTGAAATAACTGTTGACGAAATCAAT10.45045045045045046No Hit
AACGCCGGGTCACAGATTTATTTATTATAATCTAAGTACA10.45045045045045046No Hit
AACGCCGGGGAGTGCAGTGCTAACGCCGGGCACCTCTTGG10.45045045045045046No Hit
AACGCCGGGGGAAAAAAATAAGGTGTTTTATTTACAAATC10.45045045045045046No Hit
AACGCCGGGAGGCGCGAAAATTACCCACTCCCGACCCGGG10.45045045045045046No Hit
AACGCCGGGTGGCACAAAGAATAAAATGAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA10.45045045045045046No Hit
AACGCCGGGACGCCGAGTCGACGTTTTTTTTTTTTTTTTT10.45045045045045046No Hit
AACGCCGGGATCTTTATTTTTTTATGAAAAGGGAGAAGAG10.45045045045045046No Hit
AACGCCGGGGAATGCAGTGCTAACGCCGGGTCACAGATCC10.45045045045045046No Hit
AACGCCGGGTCCCTCTTCTGAGATGAGTTTTTGTTCAATC10.45045045045045046No Hit
AACGCCGCGTCAAAAATAATCTTCTGAGATGAGATCGGAA10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTTCTGAANAAAATTGGCCG10.45045045045045046No Hit
AACGCCGGGAAGAATGTTAAGCAGAGTAAAACTTTTTTTT10.45045045045045046No Hit
AACGCCGGGCAGCAGTGGTCTCAACGCAGAGTCGACTTTT10.45045045045045046No Hit
AACGCCGGGTCACAAATAATCTTCTGAAGGAGATTGTTCG10.45045045045045046No Hit
AACGCCGGGGTTTACGCTGATGAACGAATGGAAGCAAAAA10.45045045045045046No Hit
AACGCCGGGCAAAGATCCTCTTCTGAAGGAGATTGGGCGG10.45045045045045046No Hit
AACGCCGGGGATCATCTTCTGAGATCAGTTTAGGTTCCGC10.45045045045045046No Hit
AACGCCGGGGAGGTAGTGACGAAAAATAACAATACAGGAC10.45045045045045046No Hit
AACGCCGGGTCACGATCCTCTTCTGAGATGAGTTTTTGTT10.45045045045045046No Hit
AACGCCGGGGCAGTGCTATCAACGGGTCAAAGATACTCTT10.45045045045045046No Hit
AACGCCGGGTCACAGATCCTCTTCTGAAGAAGATTGGGCG10.45045045045045046No Hit
AACGCCGGGAACGCAGAGTGCAGCGCTAACGCCGGGTCAC10.45045045045045046No Hit
AACGCCGGGGGGGATAGCAATAATTTTGTTTAACTTTAAG10.45045045045045046No Hit
AACGCCGGGTCAACGATCCTCTGAAGGAGATTGGGCGGAT10.45045045045045046No Hit
AACGCCGGGACTACTCCTAGCCCTAGCCCTACACAAATAT10.45045045045045046No Hit
AACGCCGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGA10.45045045045045046No Hit
AACGCCGGGTCACAGATCTTCTTCTGAGATGAGTTTTTGC10.45045045045045046No Hit
AACGCCGGGTCAAAGATCGGAAGAATTCATATGCAATCTT10.45045045045045046No Hit
AACGCCGGGTCACATATCATCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit
AACGCCGGGGTGGCCGGTTAGCTGATGCCACTGGACACAA10.45045045045045046No Hit
AACGCCGGGTCATATATTGGAGCTGTGGCACAAGTTTGTT10.45045045045045046No Hit
AACGCCGGGCAGAATGCAGTGGTAACGCCGGGAACCAGTG10.45045045045045046No Hit
AACGCCGGGAAAAAAAAAAAAAAACAAAAAAAAAAAACTC10.45045045045045046No Hit
AACGCCGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATG10.45045045045045046No Hit
AACGCCGGGCCTTTGGTCTCGTTGTTCCAAGGTTCTTTGA10.45045045045045046No Hit
AACGCCGGGTTACAGATCCTCTTCTGAAGGAGATTGGGCG10.45045045045045046No Hit
AACGCCGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGTT10.45045045045045046No Hit
AACGCCGGGAAGCCGTGGTATCAACGCAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGTAAAAGAAAATCTTCTGAGATGAGTATTTTT10.45045045045045046No Hit
AACGCCGGGCAGCAGTGCTATCCAAAAAAAAAAAAAAAAA10.45045045045045046No Hit
AACGCCGGGTCACAGATCTTCTTCTGAGATGAGTTTTTGT10.45045045045045046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG200.034.01
ACGCCGG200.034.02
CGCCGGG200.034.03