FastQCFastQC Report
Fri 17 Jun 2016
SRR1525962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88593
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC7790.8793019764541216No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7730.8725294323479281No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7590.8567268294334766No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3140.35442980822412606No Hit
AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2970.33524093325657783No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2880.3250821170972876No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1860.20994886729199824No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.17721490411206303No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1370.15463975709141806No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1350.15238224238935358No Hit
AAAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1190.1343221247728376No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT1170.13206461007077308Illumina Single End Adapter 2 (95% over 24bp)
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1030.11626200715632161No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT1010.11400449245425712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCGC203.883522E-450.70761541
AACCAAC354.667345E-850.7076141
ACCCCGA251.9389581E-550.56181740
GCCGATT203.9391566E-450.56181338
GGACGAT150.00804186250.56181338
AGAGACC451.2369128E-1050.41685536
AGCAAGT150.008189212550.3302846
TCGCATG150.008189212550.3302846
CCGACTC251.9923998E-550.3302843
GGTTGAT301.0264721E-650.07232315
CTCGTCC204.1563727E-450.01535850
CATATCC252.0748466E-549.98692313
TTAACAG150.00841399249.98692333
CCAGTGA150.00847089749.90181729
CAGAAGT150.00848992849.8735127
GTTGATC301.063363E-649.81699416
TACGCTG150.00852808749.81699426
TTACGCT150.00854721549.78878425
ACGGGAG355.5135388E-849.6482244
AACGGGA1300.049.6482243
Produced by FastQC (version 0.11.5)