##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88593
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6598151095459	38.0	36.0	39.0	33.0	40.0
2	35.78377524183626	38.0	36.0	39.0	29.0	40.0
3	35.9583375661734	38.0	36.0	39.0	31.0	40.0
4	34.593794092084025	38.0	35.0	39.0	20.0	40.0
5	34.583894890115474	38.0	35.0	39.0	24.0	40.0
6	34.89654938877789	38.0	35.0	39.0	25.0	40.0
7	35.41433296084341	38.0	35.0	39.0	28.0	40.0
8	35.26820403417877	38.0	35.0	39.0	27.0	40.0
9	35.27394940909553	38.0	35.0	39.0	27.0	40.0
10	34.58442540607046	38.0	35.0	39.0	24.0	40.0
11	34.33518449538903	38.0	33.0	39.0	26.0	40.0
12	33.28041718871694	38.0	32.0	39.0	19.0	40.0
13	32.74443804815279	37.0	31.0	39.0	17.0	40.0
14	33.56547357014662	38.0	33.0	39.0	21.0	40.0
15	32.2530335353809	37.0	31.0	39.0	14.0	40.0
16	31.903276782590048	36.0	29.0	39.0	16.0	40.0
17	30.804871716727057	35.0	27.0	38.0	13.0	40.0
18	30.24846206810922	35.0	25.0	38.0	12.0	40.0
19	31.562256611696185	36.0	29.0	38.0	16.0	40.0
20	31.962717144695404	36.0	29.0	39.0	18.0	40.0
21	32.35400088043073	37.0	30.0	39.0	16.0	40.0
22	31.572844355648865	36.0	29.0	39.0	13.0	40.0
23	32.26179269242491	37.0	30.0	39.0	16.0	40.0
24	31.74310611447857	36.0	29.0	39.0	14.0	40.0
25	32.11516711252582	36.0	30.0	39.0	16.0	40.0
26	32.626257153499715	37.0	32.0	39.0	16.0	40.0
27	32.77271341979615	38.0	32.0	39.0	17.0	40.0
28	32.743873669477274	38.0	32.0	39.0	17.0	40.0
29	32.36688000180601	37.0	31.0	39.0	15.0	40.0
30	32.04887519329969	36.0	30.0	39.0	13.0	40.0
31	32.63834614472927	37.0	32.0	39.0	13.0	40.0
32	32.6027338503042	37.0	32.0	39.0	12.0	40.0
33	32.36878760172926	37.0	32.0	39.0	11.0	40.0
34	32.03998058537356	36.0	31.0	39.0	10.0	40.0
35	32.08307654103597	36.0	31.0	39.0	4.0	40.0
36	31.11692797399343	36.0	30.0	38.0	4.0	40.0
37	30.77606582912871	35.0	29.0	38.0	4.0	40.0
38	30.297348548982427	35.0	29.0	38.0	4.0	40.0
39	29.29687447089499	35.0	26.0	38.0	4.0	39.0
40	26.92335737586491	33.0	17.0	38.0	4.0	39.0
41	25.498504396509883	31.0	12.0	37.0	4.0	39.0
42	26.127199665887826	31.0	17.0	36.0	4.0	39.0
43	26.15185172643437	31.0	17.0	37.0	4.0	39.0
44	25.321526531441535	31.0	12.0	36.0	4.0	39.0
45	26.066980461210253	31.0	17.0	36.0	4.0	39.0
46	24.32058966284018	29.0	10.0	36.0	4.0	39.0
47	23.33608750126985	28.0	6.0	35.0	4.0	39.0
48	24.09413836307609	29.0	4.0	35.0	4.0	39.0
49	23.04929283351958	28.0	4.0	35.0	4.0	39.0
50	23.706195749099816	29.0	4.0	35.0	4.0	39.0
51	24.003657173817345	30.0	4.0	36.0	4.0	39.0
52	23.73124287471922	30.0	4.0	36.0	4.0	39.0
53	23.623830325194994	30.0	4.0	36.0	4.0	39.0
54	23.26949081755895	29.0	4.0	35.0	4.0	39.0
55	22.86699852132787	29.0	4.0	35.0	4.0	39.0
56	22.115415439143046	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.0733365946872055
1	2	4.319070249444543
1	3	4.177010588672488
1	4	6.032729512061749
1	5	5.206150087649959
1	6	5.323253604472093
1	7	4.5364253046691445
1	8	4.707357476035966
1	9	4.591940088634942
1	10	5.184473158409155
1	11	-0.9854849840261757
1	12	-7.5764938405962
1	13	-17.894422655196088
1	14	-7.742405610464619
1	15	1.1837039032325016
1	16	-1.8691981939176578
1	17	-6.8223671882552495
1	18	-5.07862101741129
1	19	-0.8263724930076393
1	20	-1.0824202274986199
1	21	6.659127713925841
1	22	7.526080261538489
1	23	6.746056611970193
1	24	7.362938551826826
1	25	6.3580321155102055
1	26	6.086242592956175
1	27	6.176967702301386
1	28	5.753542486879137
1	29	6.664381138494068
1	30	6.38274912085544
1	31	5.805467749628132
1	32	5.938303264104967
1	33	6.05590933679942
1	34	6.179458169376449
1	35	6.18285421693734
1	36	5.06524662954272
1	37	5.225722459838437
1	38	5.945092193834089
1	39	5.468302498507914
1	40	-7.273031668457033
1	41	1.7559801034540037
1	42	1.3947340942593804
1	43	-7.184474114070978
1	44	-13.551984841761776
1	45	0.18334803267438815
1	46	-3.796738855475404
1	47	-7.332331371840304
1	48	-3.6233544083119007
1	49	-11.563113531448995
1	50	-1.744648601303986
1	51	4.925543533722212
1	52	5.093484525433315
1	53	5.0547693429593
1	54	5.150487634054574
1	55	5.352873061641539
1	56	7.969965294721156
2	1	3.122433216839603
2	2	4.567302771047061
2	3	4.219921036433682
2	4	6.260832418581785
2	5	5.397921493777218
2	6	5.691085497637843
2	7	4.8183381090682005
2	8	4.950189369201716
2	9	4.9989510862783035
2	10	5.578424454559979
2	11	4.325787600420014
2	12	0.040552506615114225
2	13	1.8861154437827068
2	14	4.971459275631215
2	15	5.953440745337762
2	16	4.9800751760744895
2	17	7.505303667989839
2	18	4.795397050145667
2	19	6.259742982247666
2	20	6.351201139351343
2	21	7.393711374569989
2	22	7.922093615819719
2	23	7.261767531058961
2	24	7.663311686155186
2	25	6.9237233959501125
2	26	6.902915805839129
2	27	6.432270137493841
2	28	6.545667388685889
2	29	6.571981295603262
2	30	6.976818248899431
2	31	5.937832242950993
2	32	5.833334685943143
2	33	4.990021669870902
2	34	5.56722329113607
2	35	5.24039545809995
2	36	6.108157077303915
2	37	6.956672970443307
2	38	6.595229664376117
2	39	6.876197235350016
2	40	4.526555919916888
2	41	9.24419691413743
2	42	8.47976944382733
2	43	7.684830677759344
2	44	-3.445052398713859
2	45	7.900649682320893
2	46	7.491654703047772
2	47	3.230119531537543
2	48	7.252431137642539
2	49	0.12119518811109842
2	50	7.084691540094287
2	51	10.545732064751277
2	52	9.662907149156805
2	53	10.88440406409049
2	54	11.109639244423782
2	55	11.533745017650965
2	56	12.346438193385726
3	1	3.0629663678990653
3	2	4.421027993193334
3	3	4.250765192277839
3	4	5.9921208669754975
3	5	5.468410215581734
3	6	5.339923501739001
3	7	4.481957384529579
3	8	4.5422091914846945
3	9	4.513048830639207
3	10	5.224015705414388
3	11	4.521105440474699
3	12	5.174523798481143
3	13	5.7761530377676635
3	14	5.414213889438461
3	15	0.46941818894678633
3	16	6.977768272451794
3	17	7.8392920480308526
3	18	8.665612361150451
3	19	7.520593973361812
3	20	6.538145773664393
3	21	7.7013839651373175
3	22	8.215070375901739
3	23	7.5331277497875995
3	24	8.087012984856482
3	25	7.538299609210533
3	26	6.457341643159708
3	27	6.26070486066541
3	28	6.561644832294913
3	29	6.640248554659994
3	30	6.855321324770923
3	31	5.064626501324202
3	32	5.319578705081902
3	33	5.671241765564684
3	34	5.770658014307639
3	35	5.442206804648141
3	36	7.054585648732484
3	37	7.269044809130939
3	38	7.004406014341935
3	39	8.929341459546873
3	40	10.256819077811624
3	41	12.719248178662376
3	42	11.601266367955834
3	43	12.017793767301384
3	44	11.617233999098858
3	45	11.972163694624378
3	46	13.283606172630822
3	47	13.608451725659208
3	48	12.966973174553
3	49	13.977398195629899
3	50	12.605709995323267
3	51	12.408001374389006
3	52	12.754756089689952
3	53	12.513676107836215
3	54	12.472933844560487
3	55	13.293656159141051
3	56	12.954264577527898
4	1	3.100447873735007
4	2	4.367436015550723
4	3	4.246819770610898
4	4	6.2409290208469415
4	5	4.686312566395472
4	6	5.3224978893766846
4	7	4.277781813265399
4	8	4.719259988788657
4	9	4.8123255033335965
4	10	5.3522419095899565
4	11	5.270324575769784
4	12	6.128493879033503
4	13	6.317744357839999
4	14	5.65850124764987
4	15	6.487501371586934
4	16	7.17586131864611
4	17	8.361649437476853
4	18	8.9538747316913
4	19	7.421218665125743
4	20	6.95479874095027
4	21	6.926273000152932
4	22	7.8040889522620915
4	23	6.561567664303464
4	24	7.092109154509618
4	25	6.511914601812869
4	26	5.929148317498026
4	27	5.860984328033485
4	28	5.250614090884429
4	29	5.910838723984337
4	30	6.03294749606798
4	31	4.656686340219483
4	32	4.35007352671596
4	33	4.488272835760309
4	34	4.700051405726345
4	35	5.3621476233231355
4	36	5.717334292493788
4	37	5.977658979770425
4	38	6.481555447187571
4	39	7.22146705546994
4	40	3.199857052495169
4	41	10.753524029393922
4	42	9.613513614380292
4	43	9.474887306673402
4	44	10.616823017675216
4	45	8.843937490448809
4	46	11.744069759676687
4	47	11.766761770045207
4	48	11.424066713925018
4	49	11.814649551868595
4	50	12.234758162346118
4	51	11.62401321065401
4	52	11.17831354489298
4	53	11.01556662238496
4	54	11.418766492924782
4	55	11.755763671881478
4	56	10.788310278662209
5	1	3.270758575691275
5	2	4.748200820366165
5	3	4.483032924545569
5	4	6.500112874967506
5	5	5.850028597200115
5	6	5.843919505735009
5	7	4.941497844070042
5	8	4.976275125550629
5	9	5.304757122347496
5	10	5.414824896223578
5	11	5.456170375539635
5	12	6.111586735544076
5	13	6.674254935869563
5	14	5.993634468859046
5	15	7.355032574561175
5	16	8.0856603803439
5	17	8.722408931147736
5	18	9.483794179332264
5	19	8.236877689645532
5	20	7.1690149045335225
5	21	7.154430918184268
5	22	8.044241205072566
5	23	7.170490387150235
5	24	7.78131867916218
5	25	7.218119789030716
5	26	6.258040943859008
5	27	6.137827737788282
5	28	5.4157906864407686
5	29	6.5488399632514
5	30	6.960715930165531
5	31	5.096095032792729
5	32	5.721676607179802
5	33	5.527885122208044
5	34	5.717710961360584
5	35	5.835813198254531
5	36	7.081059175206015
5	37	7.208605248691377
5	38	7.759151269087194
5	39	7.9578129880184
5	40	9.630944951937497
5	41	12.10636106577526
5	42	11.541326308015773
5	43	11.368443117950733
5	44	11.980370862235716
5	45	11.284851007311694
5	46	12.695694084718735
5	47	13.172887290094778
5	48	12.254685462265282
5	49	13.933442151673855
5	50	13.321993711606986
5	51	12.276133242520874
5	52	12.584925919859781
5	53	12.471218565378674
5	54	12.723183594810237
5	55	12.4385113039962
5	56	11.516202639465963
6	1	3.3121933314117484
6	2	4.620471406922462
6	3	4.424853009222801
6	4	6.2979341013601555
6	5	5.611267358438873
6	6	5.695829500502143
6	7	4.89321279578499
6	8	5.093538814242891
6	9	5.217130463292264
6	10	5.438610634295031
6	11	5.072744277827823
6	12	6.315525653768713
6	13	6.490438752053379
6	14	5.342476103414963
6	15	7.540846760375356
6	16	8.20040278080059
6	17	9.1577830808076
6	18	9.642112051935854
6	19	8.273840726608569
6	20	7.783352947442996
6	21	7.540283161179374
6	22	8.103753121727344
6	23	7.483034985409116
6	24	7.767903522889878
6	25	7.531806102717027
6	26	6.933841333945111
6	27	6.561879876126138
6	28	6.561644832294913
6	29	6.52924051508052
6	30	7.114752369916257
6	31	5.3302418383681065
6	32	5.144872458947084
6	33	5.273901011081076
6	34	5.534418063781974
6	35	5.126807917820681
6	36	6.452544832405955
6	37	6.754202508574352
6	38	6.85691065256086
6	39	7.937690253609954
6	40	4.947295268287814
6	41	11.418382377796576
6	42	10.671767295599619
6	43	10.571287892224078
6	44	11.888415198851483
6	45	10.607908901798154
6	46	12.68960493577244
6	47	13.41612024761345
6	48	12.602099952536921
6	49	12.950187311276157
6	50	12.352772456671445
6	51	11.634346457876948
6	52	12.33051366544753
6	53	11.875766398497934
6	54	11.896248625018124
6	55	11.897243048442228
6	56	11.68988609886371
7	1	2.9770522819849816
7	2	4.461487533652878
7	3	4.280235721748369
7	4	6.1714415462961725
7	5	5.2416369888085015
7	6	5.557206219021722
7	7	4.591847494419689
7	8	4.906345055620562
7	9	4.8572046747950495
7	10	5.114125595524278
7	11	4.854771774141028
7	12	5.838859462816803
7	13	6.401527663142289
7	14	5.546082021306592
7	15	6.977410196938795
7	16	7.72202401670754
7	17	8.925206133944936
7	18	9.714563410101498
7	19	7.915199367967205
7	20	7.259923995442616
7	21	7.783801547554901
7	22	8.330954491785853
7	23	7.380280596940448
7	24	8.466633364476866
7	25	7.309028879939802
7	26	6.852446538264601
7	27	6.298666898627449
7	28	6.289916560566642
7	29	6.611777026188459
7	30	7.1075690770186775
7	31	5.4387523754500755
7	32	5.560837446340642
7	33	5.36005295437587
7	34	5.626801870451491
7	35	5.3463027087440445
7	36	6.710429804576641
7	37	7.38343042351655
7	38	7.542368052303974
7	39	8.447323477528894
7	40	8.533042854035397
7	41	11.32314428255848
7	42	11.08678085347032
7	43	10.585226334733946
7	44	11.74960163146649
7	45	10.781354503815187
7	46	11.821568210592854
7	47	12.445614562822051
7	48	11.667272874852696
7	49	12.101274319506022
7	50	12.315000704613979
7	51	11.681727648115281
7	52	12.333177668111535
7	53	12.072617166777274
7	54	11.946959818586464
7	55	12.249700115185007
7	56	11.495223618486943
8	1	3.3313646362973373
8	2	4.281417603582945
8	3	4.087345278857924
8	4	6.123073247927877
8	5	5.280098527270042
8	6	5.189490601306105
8	7	4.348840501412695
8	8	4.4372308365063375
8	9	4.523871341461714
8	10	4.6904659218646
8	11	4.956170375539635
8	12	5.40179652575387
8	13	6.199313210927841
8	14	5.6317463569709325
8	15	6.888249357777955
8	16	7.605473900157424
8	17	8.351196809935612
8	18	9.218642664180749
8	19	7.277087479855318
8	20	7.276241011759634
8	21	7.129955393708748
8	22	7.5949405057718735
8	23	7.573171039830886
8	24	7.87922077706428
8	25	7.018819089730016
8	26	6.540674976493037
8	27	5.638410488371033
8	28	5.722900243550324
8	29	6.419469333880773
8	30	6.697312666762269
8	31	4.75402044071814
8	32	5.221093856597051
8	33	5.118211462534379
8	34	5.3074545511041755
8	35	5.537444899886236
8	36	6.134672228819063
8	37	7.0320318221179505
8	38	6.79877830871423
8	39	6.854666134871547
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115	16	0.9654870753879905
115	17	2.6354524094086074
115	18	3.454149910557561
115	19	2.258312776298009
115	20	2.0828945031957318
115	21	4.964935630862897
115	22	5.416086750831155
115	23	4.939113068816393
115	24	4.799181251372577
115	25	4.517830947437524
115	26	4.02848788821899
115	27	3.276091647791322
115	28	4.080278372667582
115	29	3.9155167647108122
115	30	4.349815960569909
115	31	2.994468398557398
115	32	3.1871676378882228
115	33	2.9765777339441293
115	34	3.0577839318683395
115	35	3.1385647944843917
115	36	2.830983164260438
115	37	3.2919132450428528
115	38	3.362678176961925
115	39	4.392215541986175
115	40	6.082906034333362
115	41	6.377492108645438
115	42	5.519815210417718
115	43	3.4534233551048814
115	44	-3.14899976713491
115	45	2.9507068905588802
115	46	-0.9085169216661875
115	47	-8.073084129789688
115	48	-0.6300059332956742
115	49	-4.5381297546806625
115	50	-0.42732218988282966
115	51	1.3969906459869748
115	52	1.4744060049920442
115	53	1.6209601281637163
115	54	1.3224538897327065
115	55	1.9273376949965026
115	56	5.051243685376573
116	1	3.2387239436566446
116	2	4.3946210667864065
116	3	4.023492465005113
116	4	6.05575723061186
116	5	5.277934025105537
116	6	5.218278480093986
116	7	4.636069938642137
116	8	4.51666806594357
116	9	4.746382163972541
116	10	5.031807913206592
116	11	3.917642237011492
116	12	3.175389599346939
116	13	4.8332958949105205
116	14	4.854040729265304
116	15	-6.040105620577023
116	16	4.73101502569855
116	17	6.503361312100115
116	18	6.0638807594188435
116	19	5.969078821846665
116	20	5.674509410028033
116	21	7.246405610158966
116	22	7.209875570706934
116	23	7.232694849354697
116	24	7.674025971869472
116	25	7.4460918170027455
116	26	6.736562422380487
116	27	5.888410488371033
116	28	5.847359118009198
116	29	6.352369766781205
116	30	6.226316995766599
116	31	5.210513947211648
116	32	5.359838445341644
116	33	5.243969038291954
116	34	5.617411261060884
116	35	5.0370119994533376
116	36	6.735538029684868
116	37	7.1569235970097225
116	38	7.939903849839769
116	39	9.011159641365058
116	40	9.709200030192573
116	41	8.199334758748957
116	42	6.682651649341114
116	43	2.6610838105914247
116	44	1.760524042388898
116	45	7.202466724927408
116	46	-0.2614154723908264
116	47	-5.409730092522608
116	48	-0.5230701154902917
116	49	-4.536887518655817
116	50	0.9459697355830095
116	51	4.95951652590416
116	52	5.063846998780864
116	53	4.395494289654398
116	54	3.964275835902477
116	55	4.418331483816377
116	56	7.268117391380716
117	1	2.2471895521222507
117	2	3.347354752853427
117	3	2.940600048779359
117	4	4.977450352304977
117	5	4.177404924576443
117	6	4.176303171452005
117	7	3.812084047989579
117	8	3.856703339312176
117	9	3.72027987120358
117	10	3.646975461707477
117	11	-0.9433806906781008
117	12	-7.656003698713022
117	13	-8.648891053943093
117	14	-3.059892251334343
117	15	-8.79777757824898
117	16	-7.5617539337370765
117	17	-0.030677488605348913
117	18	-2.4140733852019665
117	19	-0.46337267727150433
117	20	-1.0503583148396949
117	21	5.047463811217163
117	22	5.894884389049089
117	23	4.9219364719296514
117	24	6.133990698500863
117	25	5.233746137990394
117	26	5.13691515606655
117	27	5.197052463679679
117	28	4.387041657691743
117	29	5.186584934329705
117	30	5.590690893473823
117	31	4.336792606823639
117	32	3.6321488509853808
117	33	4.308166569156153
117	34	3.842808086457712
117	35	4.261350623791959
117	36	5.2177249785384845
117	37	5.287082327168456
117	38	5.805512315448237
117	39	6.468302498507914
117	40	8.028424016083232
117	41	6.002156628237493
117	42	4.279477046166509
117	43	-3.4362706867630735
117	44	-1.5625800140484891
117	45	4.462784185244871
117	46	-2.207094484736505
117	47	-0.5395360889952698
117	48	-1.2772147363015804
117	49	-2.784506566274864
117	50	0.30187802482463155
117	51	4.1133084130293796
117	52	3.766139767740299
117	53	3.646993407820183
117	54	4.151224689517999
117	55	3.9851745173260795
117	56	6.068822858752849
118	1	1.5882619678215306
118	2	2.7444433837729036
118	3	2.430741931956071
118	4	4.405863840419961
118	5	3.295702326455931
118	6	3.6441491268601496
118	7	3.2976193345198865
118	8	3.192432102901634
118	9	3.420298297590165
118	10	3.1247716658718403
118	11	-1.3213058795485644
118	12	-8.046643094327543
118	13	-7.857109222360265
118	14	-8.38419665524074
118	15	-8.629728932588392
118	16	-10.488601178992283
118	17	-1.0984865912403237
118	18	-5.787576241291887
118	19	-1.9607008512165933
118	20	-1.977942615558323
118	21	2.800918758701961
118	22	3.6977220525832664
118	23	2.857996910477656
118	24	3.4450281040656314
118	25	2.55952465282364
118	26	3.175794831762147
118	27	3.2366691948386936
118	28	2.858020098819434
118	29	3.8434856161060047
118	30	3.6140924044375247
118	31	2.783079689926641
118	32	2.199497293955716
118	33	2.77232724724718
118	34	2.7466223484809227
118	35	2.82464526171346
118	36	3.9980824504382397
118	37	3.47945380312202
118	38	4.415241447565432
118	39	4.050392050746719
118	40	7.503087734528041
118	41	4.1141890586778835
118	42	3.835032601722066
118	43	-1.385966651384411
118	44	-4.4997460357916275
118	45	2.5639450475997627
118	46	-1.9459925868186865
118	47	-1.7726298793029898
118	48	-3.3173842590581692
118	49	-2.1377403971206377
118	50	-0.4088277057815972
118	51	2.0001703993938555
118	52	2.0710964657318236
118	53	1.502530536989152
118	54	2.3892936042038286
118	55	2.7483954497012384
118	56	5.723696638004739
119	1	2.2020224337844
119	2	3.5602424372858295
119	3	3.3510185974092934
119	4	5.350530749775622
119	5	4.313357950773366
119	6	4.371124008549266
119	7	3.904827197643293
119	8	3.5479108069424115
119	9	4.096672524018999
119	10	4.276755648398229
119	11	-0.7322996909792145
119	12	-6.411950702627507
119	13	-4.5078190876678725
119	14	-5.802289119747474
119	15	-6.816296096767498
119	16	-10.599995427263124
119	17	-0.6190544834864085
119	18	-5.990706929315184
119	19	-1.4474136287921304
119	20	-1.2784522624458354
119	21	4.096695970205424
119	22	5.261791947013556
119	23	3.583333641456907
119	24	3.154165344691773
119	25	4.197311329197866
119	26	4.959558938059928
119	27	4.209548699753146
119	28	4.105198839263554
119	29	5.109048047849729
119	30	4.914238017833956
119	31	3.6400145279317364
119	32	4.5058314767005285
119	33	3.651286111462692
119	34	3.402428682663672
119	35	3.5847471910909654
119	36	3.7270595163283033
119	37	5.119060646951652
119	38	3.918881782476241
119	39	5.626839083873765
119	40	8.329989809518938
119	41	5.518266233777993
119	42	4.347227723673285
119	43	-3.213596793357471
119	44	-1.624609523232472
119	45	3.431618873591752
119	46	-1.2841797000331034
119	47	-1.6992771308501347
119	48	-4.471734459276586
119	49	-3.658490306112263
119	50	0.23969795857952292
119	51	4.281932321490686
119	52	4.34503166777041
119	53	3.935912408121297
119	54	3.3638112598281467
119	55	4.607878522143906
119	56	6.173460016235534
120	1	2.0728976131244323
120	2	3.5217919351337486
120	3	3.147862212904272
120	4	5.252395886074041
120	5	4.337317778606945
120	6	4.786905931074372
120	7	3.899375260770988
120	8	3.86512744969707
120	9	4.102124460891304
120	10	4.147774299761217
120	11	-1.3406210683106359
120	12	-7.973069784406558
120	13	-5.951578054669309
120	14	-6.807023696505006
120	15	-1.5280608026498506
120	16	-8.968144638166997
120	17	-0.8070028335581476
120	18	-5.356847531897682
120	19	-1.0841424666687445
120	20	-1.2834737832492813
120	21	2.7080302600189086
120	22	4.981304142135507
120	23	3.791798490811278
120	24	3.9412528626257775
120	25	3.2696212287674484
120	26	5.076058220699814
120	27	4.645273233469073
120	28	4.734754075992395
120	29	5.110913184148149
120	30	5.674496267475281
120	31	4.400990137687838
120	32	4.238830041980293
120	33	4.208674920644899
120	34	3.919932269464244
120	35	3.026927965839892
120	36	5.430215900833325
120	37	4.532833961155383
120	38	4.67827919997983
120	39	6.433008380860851
120	40	7.521524960164566
120	41	-5.735679246853284
120	42	-3.0826144571014638
120	43	-4.612305545150871
120	44	-14.33135270831138
120	45	-3.881006664428334
120	46	-7.516317433175139
120	47	-6.179477991682271
120	48	-4.9925379026051395
120	49	-13.698232056470943
120	50	-3.055710936685898
120	51	1.9287042009741882
120	52	1.7411579231506131
120	53	2.1148220207468356
120	54	2.112735219655981
120	55	2.7763146770936906
120	56	4.90789330174486
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2749.0
5	858.0
6	372.0
7	199.0
8	543.0
9	553.0
10	386.0
11	340.0
12	285.0
13	274.0
14	325.0
15	376.0
16	470.0
17	611.0
18	746.0
19	915.0
20	1119.0
21	1334.0
22	1657.0
23	1904.0
24	2102.0
25	2464.0
26	2682.0
27	2738.0
28	2880.0
29	3318.0
30	3629.0
31	3965.0
32	4688.0
33	5243.0
34	5813.0
35	6267.0
36	6680.0
37	7272.0
38	7209.0
39	5622.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.57445847866084	0.3476572641179326	0.049665323445418935	0.028218933775806216
8	99.46722653031279	0.3973225875633515	0.06546792635987042	0.0699829557639994
9	96.08998453602429	1.3917578138227626	1.7631189823123723	0.7551386678405744
10	55.56533811926451	20.920388744031694	15.041820459855746	8.472452676848057
11	37.8472339801113	22.508550336934068	15.51702730464032	24.12718837831431
12	34.15055365548068	23.04583883602542	19.097445622114613	23.706161886379284
13	23.474766629417672	15.670538304380708	22.723014233630195	38.131680832571426
14	20.734143781111374	26.824918447281387	28.018014967322475	24.422922804284763
15	34.20142628768381	18.42382739424723	23.635786442330588	23.73895987573837
16	31.951974338122348	27.802249932230954	21.385651034607392	18.860124695039307
17	27.654530059271803	21.63703076488851	31.976291278577477	18.73214789726221
18	28.369058503493505	20.418091722822346	20.559186391701374	30.653663381982778
19	25.32806324110672	25.202710333145117	20.395256916996047	29.073969508752118
20	27.43464846354667	24.062249245617803	29.97977012533481	18.523332165500715
21	25.53616579375113	23.931054722774064	22.803413400758537	27.729366082716272
22	21.63489214723511	21.533303985642206	35.96333796123847	20.86846590588421
23	26.21426072037294	20.22507421579583	32.28020272482024	21.280462339010985
24	27.78931683749492	20.99042759741726	21.5548381270601	29.665417438027724
25	25.24185264203551	21.062909907774277	27.72641583979591	25.9688216103943
26	31.244283342931674	21.619973576340662	24.094086294702848	23.04165678602482
27	21.40711123158941	28.598536188669016	21.896177825969097	28.098174753772476
28	25.90870095272497	30.325777757709847	19.563597778480155	24.20192351108502
29	32.98059964726631	27.484963596074707	19.448966671188895	20.085470085470085
30	30.35508572203022	21.323213842018475	28.038670913238917	20.283029522712383
31	34.292972057578325	24.695455828394017	21.322043465989275	19.689528648038383
32	36.04321663637287	24.700543030357768	20.585479300495614	18.670761032773743
33	35.00830705591157	23.77513308242634	22.698041343143572	18.51851851851852
34	24.52021487135991	21.91348600508906	35.89708792762228	17.669211195928753
35	22.032058963170627	28.891501435644685	31.740182225136216	17.336257376048472
36	26.292606898421088	30.55160262926069	24.92489440039755	18.23089607192067
37	31.7773280725357	25.722134919109585	23.073270550461828	19.42726645789289
38	30.517217973663968	27.97977284040002	22.821168378659667	18.681840807276348
39	31.702327263684964	25.333152487199484	19.63310840595436	23.33141184316119
40	24.074474501815203	19.4004711559253	21.8381909433361	34.68686339892339
41	18.873024199986315	19.994069748876672	19.34972515566909	41.78318089546792
42	21.56030295825833	27.5968255046474	22.19089993094001	28.651971606154262
43	19.05952919873246	22.97759167043911	26.3739248528746	31.588954277953825
44	21.395939374015168	23.189325836327978	26.81010735379139	28.60462743586546
45	28.017421799875557	22.9933819786187	25.81820238701284	23.1709938344929
46	21.14095479663013	29.358411087960867	22.6832140592445	26.817420056164508
47	18.553391339860013	23.101269412712277	22.864176243037516	35.48116300439019
48	25.884093655759738	21.991935574957363	28.12383523272756	24.00013553655534
49	23.2767888465151	23.634388402910588	24.165129516674778	28.923693233899535
50	20.38563754594289	25.73027989821883	25.889737065309586	27.994345490528698
51	19.380775502808483	26.028265990215623	29.12438847617322	25.466570030802682
52	20.569709717180626	28.095351177754406	26.88310660288813	24.45183250217684
53	19.414761150101878	26.983246547430383	30.444872085125652	23.157120217342086
54	19.54396288333145	21.952019916261175	31.959941156501078	26.5440760439063
55	20.758433325786733	21.574598143536335	28.486529318541997	29.180439212134935
56	20.467531879745188	24.01588612679483	27.850507473494833	27.66607451996515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	3.5
5	6.0
6	40.5
7	75.0
8	179.5
9	284.0
10	307.5
11	331.0
12	306.5
13	261.0
14	240.0
15	236.0
16	232.0
17	256.0
18	280.0
19	277.0
20	274.0
21	282.5
22	320.0
23	349.0
24	390.5
25	432.0
26	454.5
27	477.0
28	534.5
29	673.5
30	755.0
31	845.5
32	936.0
33	1041.5
34	1147.0
35	1216.5
36	1286.0
37	1361.5
38	1583.5
39	1730.0
40	1936.0
41	2142.0
42	2619.0
43	3096.0
44	4003.5
45	4911.0
46	5233.0
47	5126.0
48	4697.0
49	4586.0
50	4475.0
51	4790.5
52	5106.0
53	5245.0
54	5661.5
55	5939.0
56	6324.5
57	6710.0
58	6818.5
59	6927.0
60	6958.5
61	6990.0
62	5871.5
63	4140.0
64	3527.0
65	3038.0
66	2549.0
67	2144.0
68	1739.0
69	1395.0
70	1051.0
71	877.0
72	570.0
73	437.0
74	335.0
75	233.0
76	191.0
77	149.0
78	102.0
79	38.0
80	21.0
81	13.5
82	6.0
83	3.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4424728816046415
16	0.06433916900883817
17	0.020317632318580477
18	0.0
19	0.04853656609438669
20	0.12416330861354735
21	0.0011287573510322486
22	0.0
23	0.0
24	0.005643786755161243
25	0.006772544106193492
26	0.04063526463716095
27	0.06433916900883817
28	0.005643786755161243
29	0.15915478649554704
30	0.05756662490264468
31	0.020317632318580477
32	0.018060117616515978
33	0.12867833801767634
34	0.18963123497341777
35	0.14786721298522457
36	0.05756662490264468
37	0.1580260291445148
38	0.22236519815335298
39	0.13545088212386983
40	0.817220322147348
41	1.0237829173862494
42	0.2979919406725136
43	0.2630004627905139
44	0.42892779339225445
45	0.2234939555043852
46	0.31718081564006184
47	0.49891074915625383
48	0.06321041165780593
49	0.2550991613332882
50	0.18963123497341777
51	0.3262108744483198
52	0.18285869086722428
53	0.28557560981115887
54	0.2517128892801914
55	0.28557560981115887
56	0.2415540731209012
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	88593.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.10517760996919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86553341863157	65.85396137392345
2	6.8482300615365865	10.149786100481979
3	1.7943093888990433	3.9890284785479664
4	0.8362273807347834	2.478751142866818
5	0.45238530433193197	1.676204666282889
6	0.25437153475903246	1.131014865734313
7	0.199536952415768	1.035070490896572
8	0.14317918722963505	0.848825527976251
9	0.09139097057210747	0.6095289695574143
>10	0.46457076707487965	5.991444019279175
>50	0.028940474014500703	1.4222342623006332
>100	0.016755011271553037	2.2055918639170136
>500	0.0045695485286053735	2.6085582382355263
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	779	0.8793019764541216	No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	773	0.8725294323479281	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	759	0.8567268294334766	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	314	0.35442980822412606	No Hit
AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	297	0.33524093325657783	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	288	0.3250821170972876	No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	186	0.20994886729199824	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	157	0.17721490411206303	No Hit
AAAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	137	0.15463975709141806	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	135	0.15238224238935358	No Hit
AAAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	119	0.1343221247728376	No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	117	0.13206461007077308	Illumina Single End Adapter 2 (95% over 24bp)
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	103	0.11626200715632161	No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	101	0.11400449245425712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0011287573510322486	0.0	0.0	0.0	0.0
10	0.005643786755161243	0.0	0.0	0.0	0.0
11	0.011287573510322486	0.0	0.0	0.0	0.0
12	0.013545088212386983	0.0	0.0	0.0	0.0
13	0.013545088212386983	0.0	0.0	0.0	0.0
14	0.025961419073741717	0.0	0.0	0.0	0.0
15	0.025961419073741717	0.0	0.0	0.0	0.0
16	0.025961419073741717	0.0	0.0	0.0	0.0
17	0.027090176424773967	0.0	0.0	0.0	0.0
18	0.03047644847787071	0.0	0.0	0.0	0.0
19	0.03160520582890296	0.0	0.0	0.0	0.0
20	0.03273396317993521	0.0	0.0	0.0	0.0
21	0.036120235233031955	0.0	0.0	0.0	0.0
22	0.0372489925840642	0.0	0.0	0.0	0.0
23	0.038377749935096454	0.0	0.0	0.0	0.0
24	0.0417640219881932	0.0	0.0	0.0	0.0
25	0.042892779339225445	0.0	0.0	0.0	0.0
26	0.045150294041289944	0.0	0.0	0.0	0.0
27	0.051922838147483434	0.0	0.0	0.0	0.0
28	0.058695382253676924	0.0	0.0	0.0	0.0
29	0.058695382253676924	0.0	0.0	0.0	0.0
30	0.05982413960470918	0.0	0.0	0.0	0.0
31	0.06433916900883817	0.0	0.0	0.0	0.0
32	0.06659668371090267	0.0	0.0	0.0	0.0
33	0.07111171311503166	0.0	0.0	0.0	0.0
34	0.07111171311503166	0.0	0.0	0.0	0.0
35	0.07336922781709616	0.0	0.0	0.0	0.0
36	0.07788425722122515	0.0	0.0	0.0	0.0
37	0.0790130145722574	0.0	0.0	0.0	0.0
38	0.08127052927432189	0.0	0.0	0.0	0.0
39	0.08239928662535415	0.0	0.0	0.0	0.0
40	0.08239928662535415	0.0	0.0	0.0	0.0
41	0.08465680132741865	0.0	0.0	0.0	0.0
42	0.0880430733805154	0.0	0.0	0.0	0.0
43	0.09368686013567663	0.0	0.0	0.0	0.0
44	0.10045940424187012	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACCCGC	20	3.883522E-4	50.707615	41
AACCAAC	35	4.667345E-8	50.70761	41
ACCCCGA	25	1.9389581E-5	50.561817	40
GCCGATT	20	3.9391566E-4	50.561813	38
GGACGAT	15	0.008041862	50.561813	38
AGAGACC	45	1.2369128E-10	50.416855	36
AGCAAGT	15	0.0081892125	50.33028	46
TCGCATG	15	0.0081892125	50.33028	46
CCGACTC	25	1.9923998E-5	50.33028	43
GGTTGAT	30	1.0264721E-6	50.072323	15
CTCGTCC	20	4.1563727E-4	50.015358	50
CATATCC	25	2.0748466E-5	49.986923	13
TTAACAG	15	0.008413992	49.986923	33
CCAGTGA	15	0.008470897	49.901817	29
CAGAAGT	15	0.008489928	49.87351	27
GTTGATC	30	1.063363E-6	49.816994	16
TACGCTG	15	0.008528087	49.816994	26
TTACGCT	15	0.008547215	49.788784	25
ACGGGAG	35	5.5135388E-8	49.648224	4
AACGGGA	130	0.0	49.648224	3
>>END_MODULE