FastQCFastQC Report
Fri 17 Jun 2016
SRR1525961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT130.37174721189591076No Hit
AAAACGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA60.17157563625965114No Hit
AAAACGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.14297969688304263No Hit
AAAACGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.14297969688304263No Hit
AAAACGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.11438375750643409No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.11438375750643409No Hit
AAAACGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC40.11438375750643409No Hit
AAAACGGGAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA40.11438375750643409No Hit
AAAACGGGGTTTTAGCCTCCCTGAGCTGCTAACTCATACA40.11438375750643409No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11438375750643409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT453.3196557E-933.7971044
CGGGGTG200.002495051833.79715
CGGGGGG357.6727156E-733.79715
CGGGGGC200.002495051833.79715
CGGGGCA251.6995634E-433.79715
CGGGGAG251.6995634E-433.79715
CGGGGAC251.6995634E-433.79715
CGGGGAA357.6727156E-733.79715
ACGGGGG1000.033.79714
GGGAAGC200.002495051833.79717
GGGGAGT200.002495051833.79716
GGGGAAG301.1474631E-533.79716
AAAACGG3600.032.388891
AACGGGG3300.032.2608683
AAACGGG3650.031.4822312
ACGGGGA1300.031.1973234
ACGGGGC555.984475E-1030.7246384
CGGGGGA451.431672E-730.0418685
CGGGGAT455.4688317E-626.2866365
Produced by FastQC (version 0.11.5)