FastQCFastQC Report
Fri 17 Jun 2016
SRR1525960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.0362694300518136No Hit
AAAACGGGGTTCGCAGATCTTTTTTTTTTTTTTTTTTTTT21.0362694300518136No Hit
AAAACGGGGCCTTTTTTTTTTTTTTTTTTTCCTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGACTCTGTGGAGGGCCCTGTGCCCCGATGGT10.5181347150259068No Hit
AAAACGGGGGTCTTATGCTGTGTGTAGGAACCCGGTCTTT10.5181347150259068No Hit
AAAACGGGGACGCATCCCCCCCGCGCCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGAGGGTTGGTGAGATGCCCCAGTGGGTAAGAGC10.5181347150259068No Hit
AAAACGGGGAAGCAGTGTTCAACCCCGAGCCTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGATGAGATGGACTTACATGACTTGCCGGGTAC10.5181347150259068No Hit
AAAACGGGGCTGTTAACGCCGAGCCCCCTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGACCAACACAAAAAACAAACCCAAAACCGACAA10.5181347150259068No Hit
AAAACGGGGGCGTAAGGAGGCAGCCCCAGAACAGGTTGCA10.5181347150259068No Hit
AAAACGGGGATGTAGGTTGACAAAAAGTATGTTGGCCTTC10.5181347150259068No Hit
AAAACGGGGGCCTTTTTTTTTTTTTTTTTTTTTTCCCCCC10.5181347150259068No Hit
AAAACGGGGGTCTTGCTCTTTCTACATGTGAAGGAACAAA10.5181347150259068No Hit
AAAACGGGAGCAGTTCAACCCCGAGTCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTAGAGATGCAGGAAAACCGGGTACCCCCCGT10.5181347150259068No Hit
AAAACGGGGGCTTTGTAGGTTTTGTTATTTGTTTTCCGCT10.5181347150259068No Hit
AAAACGGGGCCCTTAGAACCTCTTGGCAGAAGCGGGCACC10.5181347150259068No Hit
AAAACGGGGAGGCACGCCCTTGGAACAGCTCATGTGGTTT10.5181347150259068No Hit
AAAACGGGGAAACAGAACCCTTCTCAAGGGAAGGTGGGGG10.5181347150259068No Hit
AAAACGGGGTGGAACCAGCTCGTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGTGTATCGTTTCTCAATGGGTGGCATGTAGAGGG10.5181347150259068No Hit
AAAACGGGGATACGAACCCTTTAACCTCGTCATCCACCCC10.5181347150259068No Hit
AAAACGGGGAAACAAAACCCATAACGTCTGCACAGACACC10.5181347150259068No Hit
AAAACGGGGAGCTCGATTTTTTTTTTTTTTTTTTTTTTCC10.5181347150259068No Hit
AAAACGGGGCCTTCCAGGCCCGTCTACGCTACCCCCTGTC10.5181347150259068No Hit
AAAACGGGGGTCCATTCCTCTGGTAAAAAAAAAAAAAAAC10.5181347150259068No Hit
AAAACGGGGGCAAACACAAAAAAAAAAAAAAAAAAAACAA10.5181347150259068No Hit
AAAACGGGGCCGCCTTTTTTTTTCCTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAATAAAGAAAAGACCCCCCCTCCCCACAACA10.5181347150259068No Hit
AAAACGGGAACCAACGATAGGAATTCACACAAACAAATAC10.5181347150259068No Hit
AAAACGGGGGTCTTTCTCTTCAACCAGCCGGCCGCGCGGC10.5181347150259068No Hit
AAAACGGGGGAGAAAGAAAAAAAAAAAAAAAAAAAAAAAA10.5181347150259068No Hit
AAAACGGGGGGAGATGGACAGAACCCTGGAACCCCTGAGG10.5181347150259068No Hit
AAAACGGGGGTCCGAGATCGTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAAAAAAAAAACAACACCCCCCCCCCCCCCCC10.5181347150259068No Hit
AAAACGATATAAACAAAAAAACAACCACAACCATAACGAT10.5181347150259068No Hit
AAAACGGGGGAGAAGACTTCTTGTCCCCCCCCTCCTCTTC10.5181347150259068No Hit
AAAACGGGGCAGTGGTACACCCCCGAGCCGACCTTTTTTT10.5181347150259068No Hit
AAAACGGGGACACGCAGGGATGAGATGGACTTAAAGGAAT10.5181347150259068No Hit
AAAACGGGGTCAGATACTAGCTTTGTTTAGCCATGAAACA10.5181347150259068No Hit
AAAACGGGGGTTGCTGCTTTGCCAACATGGCGGCCCCCAA10.5181347150259068No Hit
AAAACGTTCTACCTACACTCCTTTCTCCTTAACTTTACGC10.5181347150259068No Hit
AAAACGGGGAAAGTGGTCCGAGGGCCGATTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGGTCAATCCGCGGCCGTAGCCGCCGAGCCAG10.5181347150259068No Hit
AAAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGATTTCCCGTTTGTATGCCACCTTTGTATTTC10.5181347150259068No Hit
AAAACGGGGGCTTGGTAAAAAACAACCACAACCCCCCCAA10.5181347150259068No Hit
AAAACGGGAAGTGGTCAACCAAAGCCGACAAAAAAAAAAA10.5181347150259068No Hit
AAAACGGGGGGGGGCTGGTGAGCTGGCCCCGTGGGTACGC10.5181347150259068No Hit
AAAACGTGGTTATTGAATTCTGTTGGCTGTCTTTAGGGTG10.5181347150259068No Hit
AAAACGGGTTCACGATCGTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTAGCAGTTCTTGGGAGAGGTTTTTTTGTTGT10.5181347150259068No Hit
AAAACGGGGAACAGTTACCCAAAAAAAAAAAAAAAAAAAA10.5181347150259068No Hit
AAAACGAGCAACCATACCAAATCCCCCCTTACTTTTATTC10.5181347150259068No Hit
AAAACGGGGACGTAGAGCCATTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGAGTACATTAATGTTTGAACCCCCCACCCATTG10.5181347150259068No Hit
AAAACGGGGGATGGGATGGACTTAAATGAATTGACGGGTA10.5181347150259068No Hit
AAAACGGGGAAGACGGATCCCCCACCCCCTCGGCCCTTCC10.5181347150259068No Hit
AAAACGGGGAAGTATAACCCGAGCCGCCTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGCCCCAATAACCTCCCCAAAAAAACAAAAAAA10.5181347150259068No Hit
AAAACGGGTTAACATAATCCTTGTCGGGCTGGGTATTAAT10.5181347150259068No Hit
AAAACGGGGAAGCAGTGGTATCCCCCCCGCGCCCCCTCTT10.5181347150259068No Hit
AAAACGGGGATGTTTCAAAAGAGAAAGAGACCTAACTCTC10.5181347150259068No Hit
AAAACGGGGTTTTTCTTGCCTTCTTCCCTTTCCTGTTGGC10.5181347150259068No Hit
AAAACGGGGACTCACGAGATCGACTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGCTTTAATCCCCTGTATTCGCCACTGATTTGT10.5181347150259068No Hit
AAAACGGGGCCGTCGATTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGTATACAAAACTCAAGTCCGCGGGGGTCTCGGG10.5181347150259068No Hit
AAAACGGGGCTGCATACAGTGTTTTGTTTGTTTCATGATG10.5181347150259068No Hit
AAAACGGGGATACAAATCCCAGCTCCCCTTACATTCTACG10.5181347150259068No Hit
AAAACGGGGTGGTCAACGCAGAGTTTTTTTTGTTTTNGTT10.5181347150259068No Hit
AAAACGGAGACATGGCAACCCAGAGTCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAAGCAGTTATACCCCGAGCCCACTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGGGCTGGAGACAATGGCCCAGCGGTTAGGAC10.5181347150259068No Hit
AAAACGGGGGATAAAGACAACACCACACCACCACACCACC10.5181347150259068No Hit
AAAACGGGGCCTCAACCCCCACGCCCACCCTAATTCTTTC10.5181347150259068No Hit
AAAACGGGGTTTATCAGAATCCCCATCCCACAACAAACTA10.5181347150259068No Hit
AAAACGCCATAACAATCCACTCCTACACGAGGACGAAACT10.5181347150259068No Hit
AAAACGGAGAGTTATCCCAGAGCCGAAAACAACAAAAAAA10.5181347150259068No Hit
AAAACGGGGAAGCATGGCACCACCCAGACCGCCTTTTTTT10.5181347150259068No Hit
AAAACGGGGATTGCAGACATGAGTCCACCCCTGCCCCCCT10.5181347150259068No Hit
AAAACGGGGTAACAAATCTCTAGAGTGCTGTTTTTTCGCG10.5181347150259068No Hit
AAAACGGCGAATAAAAATAAGGCCCCCCCGGAGCTGGTGG10.5181347150259068No Hit
AAAACGGGGATGTCACCCAGCACCACCTCTTATTTTTTCT10.5181347150259068No Hit
AAAACGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.5181347150259068No Hit
AAAACGGGGGTATCTTTTGTTGGTGTGTTTTGCTCTTCCC10.5181347150259068No Hit
AAAACGGGGATTGATATTTTTTTAACTGGTTCAATGACAA10.5181347150259068No Hit
AAAACGGGGAGGGATGAGATGGACTTACAGGAATTGACGG10.5181347150259068No Hit
AAAACGGGGGTATGTTTTAGTGTGTCTTGAGATTCCAAAC10.5181347150259068No Hit
AAAACGGGGGGGCTGGTGAGATGGCCCACTGCGTAAGAGC10.5181347150259068No Hit
AAAACGGGGAGCTGTTCAGTTTGTGCCACCTCTTCCTGAG10.5181347150259068No Hit
AAAACGGGGACCCAATAACCTCCCCCCCCCACCCCCCGCT10.5181347150259068No Hit
AAAACGGGATGGTCTCACGCCGCTCGACCTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAACCGGCACCGCCCCCCCCCCCCCCCAGCCC10.5181347150259068No Hit
AAAACGGGGAAGCTGGTATCACCCCGAGCCGCCTTTTTTT10.5181347150259068No Hit
AAAACGGGGAGAGTCACGCCGAGCCCCCTTTCTTTTTTTT10.5181347150259068No Hit
AAAACGGGGACATGGCCCCGCGTCGACTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGGCTGTTGACCTGGGAGGCGCGGTTGGAAGT10.5181347150259068No Hit
AAAACGGGGTCTCATCACCCAGCACANCAAGAAAAAAGAG10.5181347150259068No Hit
AAAACGGGTGTCCACGCAGAGTCTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGTGCACCTGGGGCTGGAGAGCTGCCCCAGTG10.5181347150259068No Hit
AAAACGGGATACTAAAACCCAGCCCCCCTACCGTTTTTGT10.5181347150259068No Hit
AAAACGGGGGAGTCGATTTTTTCTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGAAGCTGGTACAACGCAGCGCCGCCCTTTTTTT10.5181347150259068No Hit
AAAACGGGTAACCAAGAGTTGACTCCAACCTCACCACCTC10.5181347150259068No Hit
AAAACGGGGATCCCTGGTCCTGCTTTGCAACACCCGCCCT10.5181347150259068No Hit
AAAACGGGGAAATGAAACCCAATTCCTAGAGAGGTTAAGG10.5181347150259068No Hit
AAAACGGGGAGAAGAAAAGGACCACNNNNCTCCCCCTCCC10.5181347150259068No Hit
AAAACGGGGCTTTCCGTTTTTAAACACCTTTCAAAACAAA10.5181347150259068No Hit
AAAACGGGTTAACGATTCTAAACACATAAGGAATTGAGTA10.5181347150259068No Hit
AAAACGGGGCTCCAGATCGCCTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAAGACACCACATGTAAATAACCTGACATACA10.5181347150259068No Hit
AAAACGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC10.5181347150259068No Hit
AAAACGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTGGTCCGCAGAGTCGAAAAAAAAAAAAAAAA10.5181347150259068No Hit
AAAACGGGCTAACAAATTTCTGCATTATAATCCCTTACTC10.5181347150259068No Hit
AAAACGGAGACATGCCAACCCAGCGCCGCCTCTTTTTTTT10.5181347150259068No Hit
AAAACGAGGAAGATGTTCAACCCGCCCCGCCTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAAGCATTACAACCCAAAAAAAAAAAAAAAAA10.5181347150259068No Hit
AAAACGGGGCTGTCCCCGGAAAAAAAAAAAAAAAAAAAAA10.5181347150259068No Hit
AAAACGGGGGTTTATGTGTAAAGCCCAGGTGGGGGGCAGG10.5181347150259068No Hit
AAAACGGGGATACGAAACTCAAAACCTCCACCCGTTGACT10.5181347150259068No Hit
AAAACGGGGAAGCAGATACCCCAATCTCCTCTATTTCTAT10.5181347150259068No Hit
AAAACGGGGATTCATAACTCTACTAATCTCAGGGAATGAG10.5181347150259068No Hit
AAAACGGGGGAATTCTCCATAGCTCCTATTTAACTTATTA10.5181347150259068No Hit
AAAACGGGGCAAGCAGAGTCCAGTCCTACACCGGGGAGAT10.5181347150259068No Hit
AAAACGGGGCCTTTGACACCCCAGAAGAAAGTTTGAGAAA10.5181347150259068No Hit
AAAACGGGGAGGATATACAGTCCACCCAAAAAAAAAAAAC10.5181347150259068No Hit
AAAACGGGGGGGAAAAAAACAAAAAACAACAACAAACACC10.5181347150259068No Hit
AAAACGGGATCTGAGTCCCCTGTACCTATACAGATAGTTG10.5181347150259068No Hit
AAAACGGGGACGACTCCTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGCCTGGTCAACCCCGAGCCGCTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGCCCTGTCCCCCAGACCCGACTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGCCTATGCCTGCTTCAGCTGTCATCATACTGA10.5181347150259068No Hit
AAAACGGGCAATTGCTGTCATTTCTGTTGCCATCCTCACT10.5181347150259068No Hit
AAAACGGCCTGTCTAATAAAAACACCATTACATTACCATG10.5181347150259068No Hit
AAAACGGGGCAACAGCCCGGACCCCCCCCCCCCCCGGTCC10.5181347150259068No Hit
AAAACGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGATGGCAACCCAGAGTCGCCTTTTTTTTTCTT10.5181347150259068No Hit
AAAACGGGGCGGCTTTATGTTTGTTGAGAGACCTCGAGAG10.5181347150259068No Hit
AAAACGGGGATCAACAGAAGTATATAACCCCCTTCCAGGT10.5181347150259068No Hit
AAAACGCGGGACTTTTTTTCTCCTCCCCCCCCCCCCCTCC10.5181347150259068No Hit
AAAACGGGGAGCTGGACAGATGGATGACCTGGTGTGCTTT10.5181347150259068No Hit
AAAACGGGGTACGATCGATTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGTGCAAAAAAAAAAAAAAAACAAAAACACAA10.5181347150259068No Hit
AAAACGGGCCCCGTCTTAATCACCAACCCCTTTTGCTCAA10.5181347150259068No Hit
AAAACGGGGGGTGATGGTGGCCCCTCCCTTTACTCCCAGC10.5181347150259068No Hit
AAAACGGGGAAGCATGGTCCAACCCCGAGCCGCCTTTTTT10.5181347150259068No Hit
AAAACGGGACTATCACCCCCAGCCCACCTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAAGCCGTGGTATCAACCCAGAGCCCACTTTT10.5181347150259068No Hit
AAAACGGGGAAATGAATCTCTAATAATGGCGCGCAGTCAG10.5181347150259068No Hit
AAAACGTCTTCACTAAACACCTCCGCAAATAAATAGCGTG10.5181347150259068No Hit
AAAACGGGGGTTTCCGTGTTGTGTNGAGAGGCTCCAACCG10.5181347150259068No Hit
AAAACGGGGAGCATGCGCTGTTTCCNANNGTNTACATTTC10.5181347150259068No Hit
AAAACGGGGGTGCAGTGCTAAAACGGGGGAGCCCACTTTT10.5181347150259068No Hit
AAAACGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGGGGCTGGCGAGATGGNCCCGCCCTTACCGGC10.5181347150259068No Hit
AAAACGGGGAATCGTAACTCAGTGCCAATTAATGTGGGGC10.5181347150259068No Hit
AAAACGGGGATGCCCAGACCCCCCTATTCCATTTTCTTTC10.5181347150259068No Hit
AAAACGGGGAATGGATCACCCAGAGCCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAATGGTCCACCCAGAGCCCCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTCCAACAAAAAAAAAAAAACACAAAACACAA10.5181347150259068No Hit
AAAACGGTAAAACCAAACACAAACCCTAAAAAACAAGTTC10.5181347150259068No Hit
AAAACGGGAAGCAGTGGTATCAACCCCGCCCTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTGATACCCAGAGCCGCCTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAATTCCGAACCACCAGATGACGGTTCACAAC10.5181347150259068No Hit
AAAACGGGGAAGTATCAACCCAGAGTCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGACCGTCGCTTTTTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTGCCGAGTCCCCTTTTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGCAGTCCACGAGAGTCGTTTTCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGTCCAATCGACTTTTTTTCTTTTCTTTCTTTT10.5181347150259068No Hit
AAAACGGGGTATCAACCCAGAGTCCCCCTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGATGGATCCGCGCCGCCTTTTTTTTTTTTTTTT10.5181347150259068No Hit
AAAACGGGGAGCCTTTTTTTTTTTTTTTTTTTTTTTGGCC10.5181347150259068No Hit
AAAACGATTAATCAGCTCTCCGCTCTGCGAGTTTCTTTTG10.5181347150259068No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAA10.5181347150259068No Hit
AAAACGGCCACCCTCCCCCCCCCCCTTTTTTTTTTATTTT10.5181347150259068No Hit
AAAACGGGGAGCTGGTCCCCCCGCGTCGCCTTTTTTTTTT10.5181347150259068No Hit
AAAACGTTCTAACTAACGGCTAGTCCCAATGATTTAGACA10.5181347150259068No Hit
AAAACGGGGAAGTGGTCACCCACACCCCCGCTTTTTTTCT10.5181347150259068No Hit
AAAACGGGGGTGCTCCTTCCCTATAGTGTTCCACCTCCCC10.5181347150259068No Hit
AAAACGGGGCGACACAGAGTACAAACTCCCAAACAATCAC10.5181347150259068No Hit
AAAACGGGGTAGATAGGTTGATTACCCTCCCACTCCCCCC10.5181347150259068No Hit
AAAACGGGGATACAAAACACTCCANNNNNNNAAACACCAT10.5181347150259068No Hit
AAAACGAATTATTGAATCTCTTACATCGAGGATCGCGAGC10.5181347150259068No Hit
AAAACGGGGGATTCTTAAACTATTAAAGATACAACCTACT10.5181347150259068No Hit
AAAACGGGGGGGGGCTGGTGAGATGCCCCCCTGGGCCCCC10.5181347150259068No Hit
AAAACGGGGGGGGCTGGTCAGATGCCCCCGTGGGTCAGAG10.5181347150259068No Hit
AAAACGGGGACAGTGGTATCCGAAAAAAAAAAAAAAAAAA10.5181347150259068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACGG150.034.01
AAACGGG150.034.02