FastQCFastQC Report
Fri 17 Jun 2016
SRR1525959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC371.5758091993185688No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA291.2350936967632027No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT291.2350936967632027No Hit
AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG251.0647359454855196No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG160.6814310051107325No Hit
AAAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.42589437819420783No Hit
AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA80.34071550255536626No Hit
AAAACGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.2981260647359455No Hit
AAAACGGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAG60.2555366269165247No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA60.2555366269165247No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC50.21294718909710392No Hit
AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT50.21294718909710392No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG50.21294718909710392No Hit
AAAACGGGGGTCTATGAAGGAGGTGGACGAGCAGATGCTG40.17035775127768313No Hit
AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17035775127768313No Hit
AAAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12776831345826234No Hit
AAAACGGGGAGGGATCCTGAGACGACTGGACACTTCCTTA30.12776831345826234No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGC30.12776831345826234No Hit
AAAACGGGGGGCTTTCGATTTTTGGCGTTCAGTAGTCGCG30.12776831345826234No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC30.12776831345826234No Hit
AAAACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.12776831345826234No Hit
AAAACGGGGGGGCTGGAGAGATGGCTCAGAAGTTAAGAGC30.12776831345826234No Hit
AAAACGGGGGGAGTAAAAATAACATTCTTCCCTCAACATT30.12776831345826234No Hit
AAAACGGGGATCTTTATGGAAGCAGAAAGGACCTGCATTT30.12776831345826234No Hit
AAAACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.12776831345826234No Hit
AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA30.12776831345826234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGA557.2759576E-1234.0000044
CGGGGGT309.4076895E-634.05
CGGGGGG600.034.05
CGGGGAT200.00225191534.05
ACGGGGG1200.034.04
AAAACGG2300.033.260871
AAACGGG2250.033.2444462
AACGGGG2150.033.20933
ACGGGGT304.289032E-428.3333324
GGGTCAC250.006723853327.1999997
CGGGGTC250.006723853327.1999995
GGTCACA250.006723853327.1999998
GGGGTCA250.006723853327.1999996
GGGGGGG603.6712663E-825.56
Produced by FastQC (version 0.11.5)