##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525959_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2348 Sequences flagged as poor quality 0 Sequence length 40 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.48083475298126 28.0 4.0 31.0 4.0 33.0 2 16.353918228279387 16.0 4.0 28.0 4.0 32.0 3 15.28833049403748 17.0 4.0 25.0 4.0 31.0 4 12.94420783645656 9.0 4.0 22.0 4.0 30.0 5 12.25809199318569 8.0 4.0 19.0 4.0 28.0 6 13.154173764906304 8.0 4.0 22.0 4.0 30.0 7 16.037052810902896 15.0 4.0 28.0 4.0 33.0 8 15.627342419080069 14.0 4.0 27.0 4.0 33.0 9 15.730834752981261 12.0 4.0 27.0 4.0 33.0 10 14.012350936967632 4.0 4.0 25.0 4.0 32.0 11 14.480834752981261 4.0 4.0 28.0 4.0 33.0 12 14.333475298126064 4.0 4.0 27.0 4.0 33.0 13 13.19335604770017 4.0 4.0 24.0 4.0 32.0 14 14.130749574105621 4.0 4.0 28.0 4.0 34.0 15 13.732964224872232 4.0 4.0 28.0 4.0 33.0 16 11.070698466780238 4.0 4.0 20.0 4.0 30.0 17 10.291737649063032 4.0 4.0 17.0 4.0 28.0 18 10.298977853492334 4.0 4.0 17.0 4.0 29.0 19 10.080068143100512 4.0 4.0 15.0 4.0 29.0 20 10.4706132879046 4.0 4.0 18.0 4.0 30.0 21 8.912265758091992 4.0 4.0 10.0 4.0 26.0 22 8.837308347529813 4.0 4.0 10.0 4.0 26.0 23 8.679727427597955 4.0 4.0 10.0 4.0 25.0 24 8.373083475298126 4.0 4.0 8.0 4.0 23.0 25 8.813458262350936 4.0 4.0 9.0 4.0 26.0 26 8.893526405451448 4.0 4.0 8.0 4.0 27.0 27 9.007240204429301 4.0 4.0 4.0 4.0 27.0 28 9.484667802385008 4.0 4.0 4.0 4.0 29.0 29 8.519165247018739 4.0 4.0 4.0 4.0 24.0 30 8.657155025553664 4.0 4.0 4.0 4.0 26.0 31 9.695485519591141 4.0 4.0 4.0 4.0 30.0 32 9.414821124361158 4.0 4.0 4.0 4.0 30.0 33 9.392674616695059 4.0 4.0 4.0 4.0 30.0 34 9.500425894378195 4.0 4.0 4.0 4.0 31.0 35 9.377768313458262 4.0 4.0 4.0 4.0 31.0 36 9.279812606473595 4.0 4.0 4.0 4.0 31.0 37 8.971890971039182 4.0 4.0 4.0 4.0 31.0 38 8.886712095400341 4.0 4.0 4.0 4.0 31.0 39 8.720187393526405 4.0 4.0 4.0 4.0 30.0 40 8.741482112436115 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.045417931985092 1 2 9.736745385625984 1 3 4.772279296159892 1 4 12.495337166978956 1 5 9.772274450259527 1 6 11.652525252525253 1 7 0.668737481424051 1 8 -6.984055910921583 1 9 -6.877861234950785 1 10 0.023985591522905025 1 11 0.6534260515603805 1 12 0.11121018285197337 1 13 0.18076769830501327 1 14 2.2253521354267622 1 15 -0.21710548986668243 1 16 5.693995391010315 1 17 2.804026943206047 1 18 5.0255551248088555 1 19 6.215282354461458 1 20 4.477085352458486 1 21 -0.8951632529990752 1 22 -1.512882556912408 1 23 -1.8495331782645223 1 24 3.384397816114234 1 25 -2.3568074131506984 1 26 -0.3600907798669004 1 27 -1.4797468286274258 1 28 5.293188279382308 1 29 -0.9314305097887194 1 30 3.937411427709934 1 31 -0.7667781223751362 1 32 -0.18466649436798477 1 33 6.4269792810837565 1 34 6.08412429196011 1 35 2.41806174753936 1 36 5.2312867480777925 1 37 -1.4093154358079714 1 38 5.893125282677522 1 39 5.865006676573841 1 40 6.423507462686567 4 1 0.2120845986517601 4 2 4.903412052292651 4 3 1.2722792961598923 4 4 5.662003833645624 4 5 -0.894392216407141 4 6 -3.847474747474747 4 7 10.835404148090717 4 8 5.015944089078417 4 9 7.122138765049215 4 10 -4.976014408477095 4 11 15.486759384893714 4 12 10.944543516185307 4 13 10.84743436497168 4 14 9.558685468760096 4 15 17.282894510133318 4 16 14.527328724343649 4 17 15.304026943206047 4 18 5.858888458142189 4 19 4.548615687794792 4 20 12.31041868579182 4 21 11.771503413667592 4 22 11.820450776420925 4 23 18.317133488402146 4 24 13.5510644827809 4 25 12.809859253515969 4 26 23.806575886799767 4 27 18.85358650470591 4 28 16.626521612715642 4 29 16.401902823544614 4 30 16.270744761043268 4 31 19.899888544291528 4 32 20.982000172298683 4 33 16.26031261441709 4 34 21.417457625293444 4 35 24.251395080872694 4 36 11.064620081411126 4 37 8.424017897525362 4 38 5.226458616010856 4 39 11.698340009907174 4 40 6.923507462686567 5 1 2.0454179319850923 5 2 -2.2632546143740147 5 3 -0.22772070384010767 5 4 -3.837996166354376 5 5 11.772274450259527 5 6 13.31919191919192 5 7 5.668737481424049 5 8 9.015944089078417 5 9 12.122138765049215 5 10 9.023985591522905 5 11 7.986759384893714 5 12 5.111210182851975 5 13 6.847434364971679 5 14 7.558685468760096 5 15 8.11622784346665 5 16 7.027328724343649 5 17 6.137360276539381 5 18 7.358888458142189 5 19 12.21528235446146 5 20 5.477085352458486 5 21 8.104836747000924 5 22 6.487117443087591 5 23 7.1504668217354785 5 24 3.0510644827809 5 25 1.6431925868493025 5 26 6.6399092201331005 5 27 6.853586504705907 5 28 9.959854946048974 5 29 10.068569490211281 5 30 3.604078094376602 5 31 12.566555210958198 5 32 12.14866683896535 5 33 10.426979281083758 5 34 12.417457625293444 5 35 1.0847284142060278 5 36 4.2312867480777925 5 37 3.5906845641920278 5 38 5.55979194934419 5 39 4.865006676573841 5 40 3.423507462686567 6 1 5.71208459865176 6 2 6.003412052292653 6 3 -2.0277207038401084 6 4 -2.6379961663543767 6 5 -3.2943922164071413 6 6 -4.347474747474747 6 7 1.7354041480907174 6 8 4.215944089078416 6 9 4.322138765049214 6 10 -1.976014408477095 6 11 1.786759384893715 6 12 2.244543516185308 6 13 4.447434364971679 6 14 4.158685468760096 6 15 6.582894510133318 6 16 5.027328724343649 6 17 2.4040269432060466 6 18 5.958888458142189 6 19 1.3486156877947924 6 20 6.010418685791819 6 21 1.5715034136675925 6 22 2.2204507764209254 6 23 7.417133488402144 6 24 6.0510644827809 6 25 5.709859253515969 6 26 12.506575886799766 6 27 6.853586504705907 6 28 9.02652161271564 6 29 10.401902823544614 6 30 10.270744761043268 6 31 11.499888544291531 6 32 4.482000172298681 6 33 11.360312614417092 6 34 10.217457625293445 6 35 9.751395080872694 6 36 11.164620081411128 6 37 11.724017897525362 6 38 14.626458616010854 6 39 11.398340009907173 6 40 11.223507462686568 7 1 -0.12124873468157205 7 2 -2.9299212810406825 7 3 -4.894387370506774 7 4 -5.171329499687709 7 5 0.10560778359285905 7 6 -5.18080808080808 7 7 -6.497929185242616 7 8 -2.317389244254917 7 9 -1.5445279016174513 7 10 -1.976014408477095 7 11 -5.179907281772953 7 12 -1.2221231504813588 7 13 0.18076769830501327 7 14 0.22535213542676225 7 15 0.6162278434666515 7 16 2.860662057676983 7 17 2.470693609872713 7 18 1.5255551248088555 7 19 1.3819490211281256 7 20 2.81041868579182 7 21 2.438170080334258 7 22 0.9871174430875911 7 23 2.9838001550688107 7 24 -1.2822688505524331 7 25 2.8098592535159685 7 26 3.3065758867997666 7 27 5.353586504705907 7 28 3.626521612715642 7 29 1.7352361568779475 7 30 2.604078094376602 7 31 1.7332218776248638 7 32 3.6486668389653474 7 33 2.2603126144170904 7 34 3.5841242919601104 7 35 4.251395080872694 7 36 0.23128674807779248 7 37 0.9240178975253617 7 38 -0.9402080506558113 7 39 0.6983400099071746 7 40 0.0901741293532341 9 1 11.71208459865176 9 2 10.403412052292651 9 3 16.272279296159894 9 4 20.162003833645624 9 5 18.60560778359286 9 6 17.15252525252525 9 7 15.835404148090717 9 8 21.015944089078417 9 9 5.622138765049215 9 10 7.523985591522905 9 11 -8.013240615106286 9 12 -7.555456483814693 9 13 -7.152565635028321 9 14 -7.441314531239904 9 15 -7.217105489866682 9 16 -4.972671275656351 9 17 -4.195973056793953 9 18 -4.641111541857811 9 19 -4.451384312205208 9 20 -4.18958131420818 9 21 -4.228496586332408 9 22 -3.179549223579075 9 23 -3.1828665115978554 9 24 -2.9489355172191 9 25 -3.6901407464840315 9 26 -3.6934241132002334 9 27 -4.146413495294093 9 28 -4.373478387284358 9 29 -3.5980971764553864 9 30 -3.729255238956732 9 31 -5.10011145570847 9 32 -4.517999827701319 9 33 -4.23968738558291 9 34 -4.582542374706556 9 35 -4.248604919127306 9 36 -4.435379918588874 9 37 -4.075982102474638 9 38 -3.7735413839891443 9 39 -3.8016599900928254 9 40 -3.576492537313433 10 1 2.823195709762871 10 2 -3.2632546143740147 10 3 6.994501518382114 10 4 5.384226055867845 10 5 10.550052228037302 10 6 0.2080808080808083 10 7 10.77984859253516 10 8 10.349277422411749 10 9 13.122138765049215 10 10 10.579541147078462 10 11 8.653426051560382 10 12 9.88898796062975 10 13 1.0696565871939008 10 14 5.669796579871207 10 15 8.005116732355539 10 16 8.360662057676981 10 17 3.5818047209838255 10 18 6.247777347031079 10 19 5.659726798905902 10 20 7.477085352458486 10 21 3.660392302556481 10 22 3.3760063319764804 10 23 7.261577932846589 10 24 6.273286705003121 10 25 5.865414809071524 10 26 7.751020331244211 10 27 12.075808726928129 10 28 10.515410501604531 10 29 5.513013934655724 10 30 8.715189205487711 10 31 7.233221877624862 10 32 6.25977795007646 10 33 5.4269792810837565 10 34 6.417457625293444 10 35 3.8625061919838046 10 36 5.675731192522237 10 37 7.590684564192028 10 38 8.559791949344188 10 39 7.198340009907175 10 40 7.534618573797678 11 1 6.71208459865176 11 2 17.40341205229265 11 3 4.772279296159892 11 4 15.162003833645624 11 5 16.10560778359286 11 6 10.652525252525253 11 7 15.335404148090717 11 8 19.015944089078417 11 9 19.122138765049215 11 10 16.023985591522905 11 11 4.986759384893714 11 12 -1.5554564838146927 11 13 7.847434364971679 11 14 7.558685468760096 11 15 1.7828945101333176 11 16 20.027328724343647 11 17 10.804026943206047 11 18 13.35888845814219 11 19 6.548615687794792 11 20 17.81041868579182 11 21 17.771503413667592 11 22 13.820450776420925 11 23 16.817133488402146 11 24 3.0510644827809 11 25 13.309859253515969 11 26 4.3065758867997666 11 27 20.85358650470591 11 28 6.626521612715642 11 29 2.4019028235446136 11 30 7.270744761043268 11 31 9.89988854429153 11 32 8.482000172298681 11 33 3.7603126144170904 11 34 14.417457625293444 11 35 1.751395080872694 11 36 25.564620081411128 11 37 10.924017897525362 11 38 25.226458616010856 11 39 26.198340009907174 11 40 26.423507462686565 12 1 7.045417931985092 12 2 11.403412052292651 12 3 9.772279296159892 12 4 8.495337166978956 12 5 -2.894392216407141 12 6 -2.014141414141413 12 7 5.002070814757385 12 8 5.349277422411749 12 9 4.455472098382547 12 10 9.357318924856237 12 11 7.653426051560382 12 12 3.7778768495186412 12 13 9.84743436497168 12 14 2.2253521354267622 12 15 -7.217105489866682 12 16 -4.972671275656351 12 17 -4.195973056793953 12 18 -4.641111541857811 12 19 -4.451384312205208 12 20 -4.18958131420818 12 21 -4.228496586332408 12 22 -3.179549223579075 12 23 -3.1828665115978554 12 24 -2.9489355172191 12 25 -3.6901407464840315 12 26 -3.6934241132002334 12 27 -4.146413495294093 12 28 -4.373478387284358 12 29 -3.5980971764553864 12 30 -3.729255238956732 12 31 -5.10011145570847 12 32 -4.517999827701319 12 33 -4.23968738558291 12 34 -4.582542374706556 12 35 -4.248604919127306 12 36 -4.435379918588874 12 37 -4.075982102474638 12 38 -3.7735413839891443 12 39 -3.8016599900928254 12 40 -3.576492537313433 14 1 -2.28791540134824 14 2 5.403412052292651 14 3 2.7722792961598923 14 4 -2.837996166354376 14 5 16.10560778359286 14 6 -1.347474747474747 14 7 13.335404148090717 14 8 14.015944089078417 14 9 10.122138765049215 14 10 15.023985591522905 14 11 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13 17.84743436497168 16 14 18.89201880209343 16 15 17.11622784346665 16 16 16.360662057676983 16 17 13.804026943206047 16 18 13.025555124808857 16 19 3.5486156877947916 16 20 11.143752019125152 16 21 18.771503413667592 16 22 17.153784109754255 16 23 14.483800155068813 16 24 16.0510644827809 16 25 20.643192586849302 16 26 20.973242553466434 16 27 22.186919838039238 16 28 17.29318827938231 16 29 23.401902823544614 16 30 22.937411427709936 16 31 22.899888544291528 16 32 18.81533350563201 16 33 15.426979281083758 16 34 21.084124291960112 16 35 21.084728414206026 16 36 10.564620081411126 16 37 17.59068456419203 16 38 13.559791949344188 16 39 24.198340009907174 16 40 17.7568407960199 17 1 3.348448235015397 17 2 6.3125029613835615 17 3 6.135915659796256 17 4 -3.5652688936271026 17 5 6.2874259654110425 17 6 5.28888888888889 17 7 12.69904051172708 17 8 12.015944089078417 17 9 13.213047855958305 17 10 9.296712864250178 17 11 13.532213930348261 17 12 11.71727078891258 17 13 12.029252546789863 17 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17.11622784346665 18 16 8.110662057676981 18 17 11.387360276539379 18 18 4.2755551248088555 18 19 12.548615687794792 18 20 10.81041868579182 18 21 5.188170080334258 18 22 8.070450776420925 18 23 11.900466821735478 18 24 11.217731149447568 18 25 11.809859253515969 18 26 12.723242553466434 18 27 14.353586504705907 18 28 14.79318827938231 18 29 11.735236156877946 18 30 11.270744761043268 18 31 11.816555210958198 18 32 14.64866683896535 18 33 12.76031261441709 18 34 13.167457625293444 18 35 13.084728414206026 18 36 13.897953414744459 18 37 12.674017897525362 18 38 11.476458616010856 18 39 10.865006676573842 18 40 10.2568407960199 19 1 4.96208459865176 19 2 2.4034120522926514 19 3 5.522279296159892 19 4 6.912003833645624 19 5 7.605607783592859 19 6 8.652525252525253 19 7 9.335404148090717 19 8 8.015944089078417 19 9 10.622138765049215 19 10 7.773985591522905 19 11 14.486759384893714 19 12 12.694543516185307 19 13 12.84743436497168 19 14 15.308685468760096 19 15 9.782894510133318 19 16 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-7.347474747474747 112 7 -9.664595851909283 112 8 -8.984055910921583 112 9 -8.877861234950785 112 10 -7.976014408477095 112 11 -8.013240615106286 112 12 -7.555456483814693 112 13 -7.152565635028321 112 14 -7.441314531239904 112 15 -7.217105489866682 112 16 -4.972671275656351 112 17 -4.195973056793953 112 18 -4.641111541857811 112 19 -4.451384312205208 112 20 -4.18958131420818 112 21 -4.228496586332408 112 22 -3.179549223579075 112 23 -3.1828665115978554 112 24 -2.9489355172191 112 25 -3.6901407464840315 112 26 -3.6934241132002334 112 27 -4.146413495294093 112 28 -4.373478387284358 112 29 -3.5980971764553864 112 30 -3.729255238956732 112 31 -5.10011145570847 112 32 -4.517999827701319 112 33 -4.23968738558291 112 34 -4.582542374706556 112 35 -4.248604919127306 112 36 -4.435379918588874 112 37 -4.075982102474638 112 38 -3.7735413839891443 112 39 -3.8016599900928254 112 40 -3.576492537313433 117 1 -8.621248734681574 117 2 -2.9299212810406825 117 3 -10.227720703840108 117 4 -8.837996166354376 117 5 -6.894392216407141 117 6 -7.347474747474747 117 7 -9.664595851909283 117 8 -8.984055910921583 117 9 -8.877861234950785 117 10 -7.976014408477095 117 11 -8.013240615106286 117 12 -7.555456483814693 117 13 -7.152565635028321 117 14 -7.441314531239904 117 15 -7.217105489866682 117 16 -4.972671275656351 117 17 -4.195973056793953 117 18 -4.641111541857811 117 19 -4.451384312205208 117 20 -4.18958131420818 117 21 -4.228496586332408 117 22 -3.179549223579075 117 23 -3.1828665115978554 117 24 -2.9489355172191 117 25 -3.6901407464840315 117 26 -3.6934241132002334 117 27 -4.146413495294093 117 28 -4.373478387284358 117 29 -3.5980971764553864 117 30 -3.729255238956732 117 31 -5.10011145570847 117 32 -4.517999827701319 117 33 -4.23968738558291 117 34 -4.582542374706556 117 35 -4.248604919127306 117 36 -4.435379918588874 117 37 -4.075982102474638 117 38 -3.7735413839891443 117 39 -3.8016599900928254 117 40 -3.576492537313433 119 1 9.045417931985092 119 2 14.736745385625984 119 3 7.43894596282656 119 4 1.162003833645624 119 5 3.105607783592859 119 6 -7.347474747474747 119 7 -9.664595851909283 119 8 -8.984055910921583 119 9 -8.877861234950785 119 10 -7.976014408477095 119 11 -8.013240615106286 119 12 -7.555456483814693 119 13 -7.152565635028321 119 14 -7.441314531239904 119 15 -7.217105489866682 119 16 -4.972671275656351 119 17 -4.195973056793953 119 18 -4.641111541857811 119 19 -4.451384312205208 119 20 -4.18958131420818 119 21 -4.228496586332408 119 22 -3.179549223579075 119 23 -3.1828665115978554 119 24 -2.9489355172191 119 25 -3.6901407464840315 119 26 -3.6934241132002334 119 27 -4.146413495294093 119 28 -4.373478387284358 119 29 -3.5980971764553864 119 30 -3.729255238956732 119 31 -5.10011145570847 119 32 -4.517999827701319 119 33 -4.23968738558291 119 34 -4.582542374706556 119 35 -4.248604919127306 119 36 -4.435379918588874 119 37 -4.075982102474638 119 38 -3.7735413839891443 119 39 -3.8016599900928254 119 40 -3.576492537313433 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 904.0 5 116.0 6 67.0 7 43.0 8 42.0 9 73.0 10 119.0 11 110.0 12 67.0 13 76.0 14 55.0 15 34.0 16 24.0 17 30.0 18 17.0 19 36.0 20 45.0 21 77.0 22 79.0 23 80.0 24 71.0 25 70.0 26 31.0 27 35.0 28 18.0 29 15.0 30 9.0 31 2.0 32 2.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 97.91044776119404 0.9381663113006397 0.810234541577825 0.3411513859275053 8 96.7206132879046 1.6183986371379897 1.1499148211243613 0.5110732538330494 9 92.92110874200426 3.411513859275053 2.4307036247334755 1.236673773987207 10 51.14991482112436 27.342419080068144 13.841567291311755 7.666098807495741 11 35.32168726033234 26.54452492543673 20.536855560289734 17.5969322539412 12 35.689948892674614 26.61839863713799 21.465076660988075 16.226575809199318 13 22.335890878090368 24.126172208013642 24.509803921568626 29.028132992327365 14 19.53091684434968 32.28144989339019 33.646055437100216 14.541577825159916 15 32.793867120954005 27.385008517887567 26.2350936967632 13.58603066439523 16 31.175468483816015 32.027257240204435 23.253833049403745 13.543441226575808 17 26.2350936967632 28.87563884156729 32.240204429301535 12.649063032367971 18 27.938671209540033 28.91822827938671 26.66098807495741 16.48211243611584 19 23.86848847139197 34.54312553373185 22.715627668659266 18.87275832621691 20 28.534923339011925 28.747870528109026 30.3236797274276 12.393526405451448 21 21.337308347529813 34.28449744463373 25.63884156729131 18.739352640545146 22 18.82453151618399 29.38671209540034 36.41396933560477 15.374787052810904 23 23.80749574105622 27.896081771720617 30.962521294718908 17.333901192504257 24 26.405451448040886 30.3236797274276 23.46678023850085 19.804088586030662 25 22.25063938618926 28.04774083546462 26.47058823529412 23.231031543052 26 24.637681159420293 28.55924978687127 27.3231031543052 19.47996589940324 27 19.52991452991453 31.837606837606835 24.786324786324787 23.846153846153847 28 20.02556455048999 36.98338304218151 23.050703025138475 19.94034938219003 29 30.042735042735043 30.726495726495727 22.564102564102566 16.666666666666664 30 25.010651896037494 29.697486152535152 27.268853855986364 18.023008095440986 31 28.449744463373083 30.110732538330492 25.127768313458258 16.31175468483816 32 32.35042735042735 27.94871794871795 22.735042735042736 16.965811965811966 33 28.632296548785686 29.654878568385172 26.58713250958671 15.125692373242435 34 19.63373083475298 27.64054514480409 37.52129471890971 15.20442930153322 35 18.100511073253834 36.8824531516184 30.877342419080065 14.139693356047701 36 19.035424669227485 38.32693128467776 26.376440460947503 16.261203585147246 37 26.95911413969336 33.30494037478705 24.95741056218058 14.778534923339013 38 23.339011925042588 36.626916524701876 25.766609880749574 14.267461669505963 39 25.2129471890971 31.686541737649065 24.701873935264054 18.39863713798978 40 21.592844974446336 29.770017035775126 24.531516183986373 24.10562180579216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 1.0 7 0.0 8 0.0 9 8.0 10 16.0 11 33.0 12 50.0 13 50.0 14 41.0 15 32.0 16 31.5 17 31.0 18 31.0 19 26.0 20 21.0 21 24.0 22 27.0 23 27.0 24 30.0 25 33.0 26 33.0 27 33.0 28 33.0 29 49.5 30 66.0 31 67.5 32 69.0 33 69.0 34 75.5 35 82.0 36 95.5 37 109.0 38 109.0 39 114.5 40 120.0 41 162.0 42 204.0 43 204.0 44 173.0 45 142.0 46 150.5 47 159.0 48 159.0 49 187.5 50 216.0 51 223.0 52 230.0 53 230.0 54 214.0 55 198.0 56 200.0 57 202.0 58 202.0 59 197.5 60 193.0 61 130.0 62 67.0 63 67.0 64 50.5 65 34.0 66 20.5 67 7.0 68 7.0 69 5.5 70 4.0 71 2.5 72 1.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.12776831345826234 8 0.0 9 0.12776831345826234 10 0.0 11 0.04258943781942078 12 0.0 13 0.08517887563884156 14 0.12776831345826234 15 0.0 16 0.0 17 0.0 18 0.0 19 0.2555366269165247 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.08517887563884156 26 0.08517887563884156 27 0.34071550255536626 28 0.04258943781942078 29 0.34071550255536626 30 0.04258943781942078 31 0.0 32 0.34071550255536626 33 0.04258943781942078 34 0.0 35 0.0 36 0.21294718909710392 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2348.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.75638841567292 #Duplication Level Percentage of deduplicated Percentage of total 1 95.8253358925144 85.0511073253833 2 2.927063339731286 5.195911413969336 3 0.527831094049904 1.405451448040886 4 0.09596928982725528 0.34071550255536626 5 0.14395393474088292 0.6388415672913117 6 0.09596928982725528 0.5110732538330494 7 0.04798464491362764 0.2981260647359455 8 0.04798464491362764 0.34071550255536626 9 0.0 0.0 >10 0.28790786948176583 6.218057921635435 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 37 1.5758091993185688 No Hit AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 1.2350936967632027 No Hit AAAACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 29 1.2350936967632027 No Hit AAAACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 25 1.0647359454855196 No Hit AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 16 0.6814310051107325 No Hit AAAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.42589437819420783 No Hit AAAACGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA 8 0.34071550255536626 No Hit AAAACGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 7 0.2981260647359455 No Hit AAAACGGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAG 6 0.2555366269165247 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA 6 0.2555366269165247 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC 5 0.21294718909710392 No Hit AAAACGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 5 0.21294718909710392 No Hit AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 5 0.21294718909710392 No Hit AAAACGGGGGTCTATGAAGGAGGTGGACGAGCAGATGCTG 4 0.17035775127768313 No Hit AAAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.17035775127768313 No Hit AAAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.12776831345826234 No Hit AAAACGGGGAGGGATCCTGAGACGACTGGACACTTCCTTA 3 0.12776831345826234 No Hit AAAACGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGC 3 0.12776831345826234 No Hit AAAACGGGGGGCTTTCGATTTTTGGCGTTCAGTAGTCGCG 3 0.12776831345826234 No Hit AAAACGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC 3 0.12776831345826234 No Hit AAAACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 3 0.12776831345826234 No Hit AAAACGGGGGGGCTGGAGAGATGGCTCAGAAGTTAAGAGC 3 0.12776831345826234 No Hit AAAACGGGGGGAGTAAAAATAACATTCTTCCCTCAACATT 3 0.12776831345826234 No Hit AAAACGGGGATCTTTATGGAAGCAGAAAGGACCTGCATTT 3 0.12776831345826234 No Hit AAAACGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 3 0.12776831345826234 No Hit AAAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 3 0.12776831345826234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGGA 55 7.2759576E-12 34.000004 4 CGGGGGT 30 9.4076895E-6 34.0 5 CGGGGGG 60 0.0 34.0 5 CGGGGAT 20 0.002251915 34.0 5 ACGGGGG 120 0.0 34.0 4 AAAACGG 230 0.0 33.26087 1 AAACGGG 225 0.0 33.244446 2 AACGGGG 215 0.0 33.2093 3 ACGGGGT 30 4.289032E-4 28.333332 4 GGGTCAC 25 0.0067238533 27.199999 7 CGGGGTC 25 0.0067238533 27.199999 5 GGTCACA 25 0.0067238533 27.199999 8 GGGGTCA 25 0.0067238533 27.199999 6 GGGGGGG 60 3.6712663E-8 25.5 6 >>END_MODULE