FastQCFastQC Report
Fri 17 Jun 2016
SRR1525958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43191
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14533.364126785672941No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3970.9191729758514505No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2130.4931582968674029No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2060.476951216688662No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1770.4098075988053067No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1540.3565557639323007No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1190.275520363038596No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC990.22921441967076475No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC980.2268991225023732No Hit
GCGAATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT970.2245838253339816No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC830.19216966497649973No Hit
GCGAATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC710.16438609895580097No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC690.15975550461901786No Hit
GCGAATGGGAGTGCTGCGAATGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTC600.1389178301034938No Hit
GCGAATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA560.12965664142992753No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC540.1250260470931444No Hit
GCGAATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT460.1065036697460119No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT450.10418837257762034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATTC150.00792675650.70129841
CGTCTCA402.2791937E-950.70129841
AGCCCCC150.00796387950.6415138
CCAACGG150.00796387950.6415140
TCGTCTC354.6389687E-850.64150640
TATGCCC150.00803851450.52235435
TCTCGTG150.00803851450.52235435
ATGCCTG251.9309704E-550.5223536
TATGCTC354.72628E-850.52234635
ATGCTCG354.72628E-850.52234636
GTCTCGT150.00811366750.40375534
CACGATT204.070533E-450.1682247
CAATTCG150.00830383950.10968842
ATTGCTC150.00830383950.10968846
ACAGTCT150.00834226750.0512814
CAGTCTT150.00834226750.0512815
AATTCGC150.00834226750.0512843
GTAGTCG150.00834226750.0512814
TGGTAGT150.008380827549.99301512
ATGGTAG150.008380827549.99301511
Produced by FastQC (version 0.11.5)