##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43191
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.75909332962886	38.0	35.0	39.0	29.0	40.0
2	35.60271815887569	38.0	35.0	39.0	27.0	40.0
3	35.61482716306638	38.0	35.0	39.0	27.0	40.0
4	32.88687458035239	38.0	33.0	39.0	10.0	40.0
5	33.05156166794008	38.0	31.0	39.0	15.0	40.0
6	35.411868213285175	38.0	35.0	39.0	26.0	40.0
7	35.89324164756547	38.0	35.0	39.0	29.0	40.0
8	35.516519645296476	38.0	35.0	39.0	28.0	40.0
9	35.444930656849806	38.0	35.0	39.0	27.0	40.0
10	34.98985899840245	38.0	35.0	39.0	25.0	40.0
11	35.216688661989764	38.0	35.0	39.0	27.0	40.0
12	34.24130027088977	38.0	33.0	39.0	23.0	40.0
13	33.683985089486235	38.0	32.0	39.0	20.0	40.0
14	34.24336088536964	38.0	33.0	39.0	23.0	40.0
15	33.145331203259936	38.0	32.0	39.0	18.0	40.0
16	32.77611076381653	37.0	30.0	39.0	19.0	40.0
17	31.871732536871107	36.0	28.0	38.0	16.0	40.0
18	31.268435553703316	36.0	27.0	38.0	14.0	40.0
19	32.23567409877058	36.0	29.0	38.0	18.0	40.0
20	32.90724919543423	37.0	30.0	39.0	21.0	40.0
21	33.179736519182235	38.0	31.0	39.0	20.0	40.0
22	32.49125975318932	37.0	30.0	39.0	17.0	40.0
23	33.19080363964715	38.0	31.0	39.0	20.0	40.0
24	32.55020721909657	37.0	30.0	39.0	17.0	40.0
25	32.93500960848325	37.0	31.0	39.0	19.0	40.0
26	33.4086731031928	38.0	33.0	39.0	20.0	40.0
27	33.718529323238634	38.0	33.0	39.0	22.0	40.0
28	33.60239401727212	38.0	33.0	39.0	23.0	40.0
29	33.13380102336135	38.0	32.0	39.0	19.0	40.0
30	32.849667754856334	37.0	31.0	39.0	18.0	40.0
31	33.53666273066148	38.0	33.0	39.0	21.0	40.0
32	33.43911926095714	38.0	33.0	39.0	21.0	40.0
33	33.23423861452618	38.0	33.0	39.0	19.0	40.0
34	33.039128522145816	37.0	33.0	39.0	18.0	40.0
35	32.93639878678428	37.0	32.0	39.0	18.0	40.0
36	32.49556620592253	36.0	32.0	38.0	17.0	40.0
37	32.21740640411197	36.0	31.0	38.0	15.0	40.0
38	31.50200273205066	36.0	30.0	38.0	10.0	40.0
39	30.376930379014148	35.0	27.0	38.0	4.0	40.0
40	28.502396332569287	34.0	22.0	38.0	4.0	39.0
41	26.52307193628302	32.0	17.0	38.0	4.0	39.0
42	27.41652196059364	32.0	21.0	38.0	4.0	39.0
43	27.49160704776458	33.0	21.0	38.0	4.0	39.0
44	26.469519112778126	32.0	17.0	38.0	4.0	39.0
45	27.348591141673033	33.0	21.0	38.0	4.0	39.0
46	25.282744090203977	30.0	15.0	36.0	4.0	39.0
47	24.39913407885902	29.0	10.0	36.0	4.0	39.0
48	25.115162881155797	30.0	15.0	36.0	4.0	39.0
49	24.090389201454006	29.0	9.0	36.0	4.0	39.0
50	24.6620129193582	29.0	11.0	36.0	4.0	39.0
51	24.963348845824363	30.0	4.0	36.0	4.0	39.0
52	24.803431270403557	30.0	4.0	36.0	4.0	39.0
53	24.560950197957908	30.0	4.0	36.0	4.0	39.0
54	24.242782061077538	30.0	4.0	36.0	4.0	39.0
55	23.90285013081429	30.0	4.0	36.0	4.0	39.0
56	22.946146187863214	29.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	5.019140746184554
1	2	4.537339288015062
1	3	4.506644125510704
1	4	7.622158069790395
1	5	8.06216015180269
1	6	4.198796135731229
1	7	3.9941171022619386
1	8	4.554554328664025
1	9	4.729014021407309
1	10	4.839187575339565
1	11	-0.9236866520494758
1	12	-8.308333328567027
1	13	-20.089352451372854
1	14	-9.2169685668778
1	15	-0.556052719784951
1	16	-2.0805145386358923
1	17	-6.369434973704813
1	18	-6.963212703510209
1	19	-0.8835526842887589
1	20	-1.3850875631043067
1	21	5.522724451500576
1	22	6.576612093312772
1	23	5.150254298878632
1	24	6.539799702927862
1	25	5.365750075724943
1	26	5.819419847590488
1	27	5.539122435754962
1	28	5.295033649739381
1	29	5.4286750359381095
1	30	5.739983997123357
1	31	3.773815488818226
1	32	3.339706912730829
1	33	4.494196187113577
1	34	4.177348769287438
1	35	4.291790984505656
1	36	2.3024057926613217
1	37	2.629211431709944
1	38	3.03019269813446
1	39	3.483724701086132
1	40	-12.172853387336426
1	41	-3.100620930793763
1	42	-4.2049144737279605
1	43	-10.82401071019439
1	44	-15.846965680693483
1	45	-5.0844556682991
1	46	-7.144404692291168
1	47	-10.47229108000731
1	48	-7.290939346062741
1	49	-14.473648783622558
1	50	-5.887734180045721
1	51	-0.241111122329432
1	52	-0.6041998487654041
1	53	0.2818900290492685
1	54	0.22414201164301417
1	55	0.4147158139007949
1	56	3.8771688903842296
2	1	4.811348538392352
2	2	4.473270023945794
2	3	5.139977458844037
2	4	7.758954606586926
2	5	8.672982662625198
2	6	4.758969295904393
2	7	5.1599179680628
2	8	5.578363852473544
2	9	5.668840861234145
2	10	5.753906190058181
2	11	3.650339321976503
2	12	-1.4005411207748182
2	13	0.8864051243847229
2	14	4.274806324897092
2	15	4.978579314847085
2	16	4.536801478680129
2	17	6.568660264390427
2	18	4.335055694758189
2	19	6.050646449910378
2	20	5.576384298367554
2	21	6.4616854904616154
2	22	7.39825711495779
2	23	6.3822889309132655
2	24	7.2077650708932275
2	25	6.317265227240089
2	26	6.201670929841569
2	27	5.656438453070983
2	28	5.757371312077048
2	29	6.155514862777938
2	30	6.233490490629844
2	31	4.822733237735978
2	32	4.514598687622605
2	33	4.782940775858169
2	34	4.867824959763627
2	35	4.9350810277957
2	36	5.90153999179552
2	37	6.688951691450203
2	38	7.115041182982946
2	39	7.564244181605613
2	40	4.5600470455640085
2	41	7.659552229379397
2	42	6.613700244886758
2	43	5.168629982446301
2	44	-4.546099879827683
2	45	6.488704504861076
2	46	5.371612623726147
2	47	3.2718647641485354
2	48	5.170965415842023
2	49	-2.166289476263252
2	50	6.045166252854713
2	51	7.664949483731174
2	52	7.9784841339185775
2	53	8.007431154590392
2	54	8.216349803850807
2	55	8.42856862775361
2	56	9.245134258349594
3	1	4.688332665376478
3	2	4.096682722358494
3	3	4.617755236621818
3	4	7.1410974637297855
3	5	8.223776313418849
3	6	4.710159772094869
3	7	4.728965587110423
3	8	5.151776550886247
3	9	5.31487260726589
3	10	5.221763332915323
3	11	3.5384345600717353
3	12	3.5780303077966096
3	13	4.44473845771806
3	14	4.166869816960585
3	15	-1.102769891502124
3	16	5.236404653283298
3	17	6.380565026295191
3	18	7.034262043964539
3	19	6.18715438641831
3	20	5.440670012653268
3	21	6.19065374442987
3	22	6.9831777498784255
3	23	5.960860359484695
3	24	7.284749197877357
3	25	6.211709671684535
3	26	6.036591564762205
3	27	4.689374961007488
3	28	5.066498296204031
3	29	5.9539275611906355
3	30	4.973569855709211
3	31	4.727098317101056
3	32	3.8447574177813344
3	33	4.769448712366103
3	34	5.302348769287438
3	35	3.7299222976369713
3	36	5.634476499732028
3	37	6.0635548660533765
3	38	6.084485627427387
3	39	7.740037832399263
3	40	7.958459743976707
3	41	11.813520483347656
3	42	10.856954213140728
3	43	11.130534744351067
3	44	10.862630278902476
3	45	11.47362513978171
3	46	12.024787226900749
3	47	12.56432508160885
3	48	12.306282876159479
3	49	13.155139095165321
3	50	11.951912284600741
3	51	11.392727261508952
3	52	12.092769848204291
3	53	11.784415281574521
3	54	11.560000597501599
3	55	11.240473389658373
3	56	11.566562829778167
4	1	4.958119575574344
4	2	4.964072372282388
4	3	5.066004856104314
4	4	7.290070066469511
4	5	8.015252721333614
4	6	5.1182647949259135
4	7	4.6113856784346225
4	8	5.378755241906035
4	9	5.035572759472892
4	10	5.096763332915323
4	11	4.923137756418768
4	12	4.903372773550032
4	13	5.232980466850478
4	14	5.361694778908834
4	15	5.125350352029077
4	16	6.825255490421807
4	17	7.406820734057742
4	18	8.348950019611415
4	19	6.8553431231002016
4	20	6.875601519502585
4	21	6.820980989483136
4	22	7.46224928716914
4	23	6.704011044416202
4	24	7.3490566560204265
4	25	6.709046049157902
4	26	5.306758992463877
4	27	5.383248629196224
4	28	4.564024932429298
4	29	5.692892553580283
4	30	5.5681284554048
4	31	4.804533629125412
4	32	4.687984206213603
4	33	4.865719640828811
4	34	5.76410676015502
4	35	4.6384078379718225
4	36	5.513281674770081
4	37	5.804802963465864
4	38	6.138328854215818
4	39	6.844870404697591
4	40	2.0809863802019706
4	41	9.832546358537915
4	42	8.302349951344684
4	43	8.338297301428689
4	44	10.113391313910089
4	45	8.459545992140917
4	46	10.536583269518715
4	47	10.999066329706267
4	48	9.816366590010318
4	49	10.56952265680916
4	50	10.420900108040623
4	51	10.714264552224321
4	52	9.777701355053605
4	53	10.972010410950471
4	54	9.80695645747116
4	55	10.800975672763393
4	56	9.871357350326111
5	1	5.3279825892729775
5	2	4.977771002419374
5	3	5.038607595830342
5	4	7.6873303404421165
5	5	8.796074639141832
5	6	5.049771644240984
5	7	4.84426239076339
5	8	5.543138803549866
5	9	5.747901526596181
5	10	5.096763332915323
5	11	4.471082961898219
5	12	4.793783732454145
5	13	5.479555809316231
5	14	5.046626285758151
5	15	6.591103776686612
5	16	7.660871928777976
5	17	7.8725741587152775
5	18	7.992785636049774
5	19	7.430685588853624
5	20	6.738615218132718
5	21	6.505912496332453
5	22	7.133482163881471
5	23	6.005380907429899
5	24	5.869604601225912
5	25	6.640552898472965
5	26	5.430046663696757
5	27	4.97228972508664
5	28	4.837997535169023
5	29	5.418919950840557
5	30	5.253059962254117
5	31	5.4346706154267785
5	32	4.852367767857441
5	33	4.180788133979501
5	34	4.435339636867351
5	35	5.049366742081414
5	36	5.677665236413919
5	37	6.133570086753533
5	38	6.069835703530888
5	39	6.995555336204436
5	40	8.176876791160876
5	41	10.736655947579006
5	42	10.494130773262487
5	43	9.858845246634168
5	44	9.880514601581321
5	45	10.596532293510784
5	46	11.974939433902271
5	47	11.094956740665168
5	48	11.008147411928128
5	49	11.66541306776806
5	50	12.338708327218708
5	51	11.344401538525695
5	52	11.969482176971418
5	53	11.506256986292936
5	54	12.341203032813624
5	55	12.061249645366136
5	56	12.049439542106935
6	1	5.0113485383923475
6	2	4.916127166802937
6	3	5.339977458844039
6	4	7.530383178015498
6	5	8.70155409119663
6	6	5.258969295904393
6	7	5.074203682348518
6	8	5.406935281044973
6	9	5.754555146948434
6	10	5.496763332915322
6	11	5.336053607690786
6	12	5.8851731649394665
6	13	6.514976552956156
6	14	5.4605206106113755
6	15	6.650007886275656
6	16	7.079658621537266
6	17	8.382945978676144
6	18	8.820769980472477
6	19	7.722075021338949
6	20	6.876384298367551
6	21	6.7473997761759
6	22	7.555399972100648
6	23	7.582288930913268
6	24	7.72205078517894
6	25	7.074408084382949
6	26	6.2730995012701385
6	27	5.71358131021384
6	28	5.471657026362763
6	29	5.7269434342065075
6	30	5.719204776344132
6	31	5.66559038059312
6	32	5.3717415447654595
6	33	5.225797918715312
6	34	4.739253531192201
6	35	5.863652456367131
6	36	7.072968563224091
6	37	6.631808834307346
6	38	7.143612611554371
6	39	8.521387038748468
6	40	4.160047045564006
6	41	11.345266515093687
6	42	10.113700244886754
6	43	10.682915696732017
6	44	10.711042977315177
6	45	10.088704504861077
6	46	12.44304119515472
6	47	12.94329333557711
6	48	12.699536844413451
6	49	13.97656766659389
6	50	13.830880538569001
6	51	12.236378055159747
6	52	11.721341276775718
6	53	12.821716868876106
6	54	12.487778375279376
6	55	12.742854342039326
6	56	10.802277115492451
7	1	4.711135318776144
7	2	4.74448537575816
7	3	4.563858055858965
7	4	7.314604926416351
7	5	8.195583941942896
7	6	4.5466025581645155
7	7	4.056080014971116
7	8	4.64659412965905
7	9	4.463297151212828
7	10	4.649002138885471
7	11	4.114305206837905
7	12	4.32803030779661
7	13	4.538643930354873
7	14	5.344315919779824
7	15	5.952992960902517
7	16	6.461321734543663
7	17	7.1181272153499116
7	18	7.946782773649446
7	19	7.009708283599071
7	20	6.205595385787593
7	21	5.90603517063219
7	22	5.865847733294675
7	23	5.255636478887677
7	24	6.456805582620309
7	25	6.382936869031138
7	26	5.17672423474562
7	27	4.804839305949443
7	28	4.206838263036531
7	29	5.6595660354858275
7	30	5.227093902143704
7	31	4.335099975475853
7	32	3.9227010330383827
7	33	4.364390669248362
7	34	2.592771654859579
7	35	2.541904055514248
7	36	4.3394930834799545
7	37	4.7716809025376286
7	38	4.3881755113411565
7	39	5.094308147490477
7	40	5.90290418842115
7	41	9.247185491639524
7	42	8.47532071396991
7	43	9.461380515495343
7	44	10.362215685204301
7	45	8.341369750063635
7	46	9.923211770847686
7	47	10.24073470018265
7	48	8.631946226076565
7	49	8.912601781732484
7	50	7.919366679293944
7	51	8.058979334477442
7	52	8.659934027308768
7	53	8.792292561839858
7	54	7.945987330503257
7	55	7.754154981698171
7	56	7.309100143211005
8	1	4.81354634059015
8	2	4.4776656283413985
8	3	4.6938236126901955
8	4	8.26225130988363
8	5	8.278477168119704
8	6	4.686441823376917
8	7	4.699478407623239
8	8	4.859682533792231
8	9	5.449060641453926
8	10	4.558301794453783
8	11	4.9448448164819965
8	12	4.497261077027382
8	13	5.34025127823088
8	14	5.65063050072127
8	15	6.026930963198733
8	16	6.530208072086719
8	17	7.1587701545003135
8	18	8.314176573879067
8	19	6.288009087273014
8	20	6.267593089576348
8	21	6.789158017934138
8	22	6.970784587485266
8	23	6.795475744100077
8	24	7.51765518078334
8	25	6.271111381086246
8	26	5.48189071006135
8	27	4.926768123400649
8	28	4.693635048340781
8	29	6.190679697942773
8	30	5.9609630181023725
8	31	5.441414556417296
8	32	5.031082204106124
8	33	4.366457259374652
8	34	5.118374410313084
8	35	4.7416744343891075
8	36	4.248792739048266
8	37	5.251589054087571
8	38	4.8304257983675605
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115	36	5.20947649973203
115	37	5.130221532720046
115	38	5.859485627427386
115	39	7.259482276843709
115	40	7.6362375217544844
115	41	9.224631594458767
115	42	8.39306532425184
115	43	6.719423633239952
115	44	-1.631814165541968
115	45	7.401402917559487
115	46	0.8081205602340837
115	47	-8.832897140613369
115	48	0.43128287615948224
115	49	-2.328194238168013
115	50	1.565801173489632
115	51	3.8871717059533957
115	52	4.248325403759846
115	53	5.012193059352299
115	54	3.232222819723823
115	55	4.068251167436149
115	56	7.061007274222611
116	1	4.686348538392352
116	2	4.558984309660083
116	3	4.179263173129755
116	4	5.944668892301216
116	5	6.983696948339482
116	6	3.8411121530472485
116	7	4.288489396634233
116	8	4.328363852473544
116	9	4.908126575519859
116	10	3.953906190058177
116	11	2.514625036262217
116	12	3.4887445935108943
116	13	4.432833695813294
116	14	3.49623489632566
116	15	-3.2714206851529184
116	16	3.5975157643944122
116	17	5.475803121533286
116	18	4.738627123329614
116	19	5.072075021338943
116	20	4.851384298367552
116	21	6.24025691903304
116	22	6.598257114957793
116	23	5.889431788056125
116	24	6.264907928036084
116	25	5.642265227240092
116	26	4.062385215555857
116	27	3.752867024499551
116	28	3.4573713120770435
116	29	4.023372005635078
116	30	4.947776204915559
116	31	4.09416180916455
116	32	3.846741544765461
116	33	4.350797918715312
116	34	4.421396388335058
116	35	4.2457953135099835
116	36	5.787254277509806
116	37	5.24609454859306
116	38	5.336469754411514
116	39	5.767815610177042
116	40	7.635047045564004
116	41	7.6309808008079685
116	42	5.388700244886756
116	43	0.04720141101773123
116	44	-0.813957022684825
116	45	4.981561647718216
116	46	0.4216126237261477
116	47	-6.806706664422894
116	48	0.22096541584202
116	49	-4.505575190548964
116	50	1.1344519671404285
116	51	4.472092340874031
116	52	4.271341276775718
116	53	5.246716868876106
116	54	4.15920694670795
116	55	4.782140056325037
116	56	7.370134258349594
117	1	3.6904694175132278
117	2	3.2366399873157548
117	3	3.4681825870491707
117	4	6.75968720731953
117	5	6.822066911709445
117	6	3.783877720812818
117	7	3.555888664033496
117	8	4.234041508151201
117	9	3.9772657696590556
117	10	3.712147948299936
117	11	1.0063832780204578
117	12	-6.543764563998263
117	13	-8.346928208948608
117	14	-1.892959242868475
117	15	-10.373069036801269
117	16	-6.956971415092767
117	17	0.29723169296185503
117	18	-2.7371054774029844
117	19	0.32390652317044655
117	20	-0.09138126939801339
117	21	4.717363146139267
117	22	5.775912792613465
117	23	4.210860359484695
117	24	4.9022705653987195
117	25	5.312137022111887
117	26	4.220352248522886
117	27	4.752409149041675
117	28	3.9756863303920653
117	29	3.770166877429947
117	30	4.966091223230581
117	31	2.867055582058324
117	32	3.2721078451317602
117	33	3.617739310656699
117	34	2.6517077436464156
117	35	2.6647513574660273
117	36	3.4539209441764704
117	37	3.764409566908082
117	38	4.450938618880382
117	39	5.418456635818071
117	40	6.287519573036537
117	41	4.561383731210899
117	42	4.806740537927048
117	43	-5.52331140949509
117	44	-0.7805321142599162
117	45	1.522770438927008
117	46	-5.460255508141984
117	47	-3.6226407303569594
117	48	-1.6627342178576114
117	49	-5.723066033039807
117	50	-1.4786432709548123
117	51	3.3264879452696334
117	52	3.9645647199991636
117	53	3.940398187557424
117	54	3.7159835034845052
117	55	3.618678517863497
117	56	7.072973086188423
118	1	3.2918680189118277
118	2	3.567642318318086
118	3	3.3399774588440394
118	4	6.288824736457059
118	5	5.668220757863292
118	6	3.04728098421608
118	7	2.7819958901407205
118	8	3.175766449876143
118	9	3.6835594759527623
118	10	3.278581514733503
118	11	-1.0448987732615933
118	12	-5.187121207354906
118	13	-8.771170633191034
118	14	-9.2169685668778
118	15	-8.146961810694041
118	16	-9.716878174999525
118	17	-1.823980428250266
118	18	-7.266243006540513
118	19	-2.6411284418645167
118	20	-1.2941784721952168
118	21	1.659088087864209
118	22	2.167521184221858
118	23	2.1199512685756012
118	24	2.221617884746042
118	25	2.6687803787552404
118	26	3.0315410597116994
118	27	1.2209406175731417
118	28	3.0526094073151384
118	29	3.1104932177562894
118	30	2.55816581530517
118	31	1.8647245797273193
118	32	2.2184947915187117
118	33	1.4638931568105455
118	34	0.9803790723177421
118	35	0.6857303784450508
118	36	3.6054360956916263
118	37	3.598908401406913
118	38	3.7726169405587022
118	39	4.514027731389167
118	40	6.387752673269635
118	41	4.687257857085026
118	42	1.7950855262720395
118	43	-0.5512834374671165
118	44	-1.589389923117725
118	45	3.930695846852416
118	46	-1.7959198438063204
118	47	1.5731634654472373
118	48	-0.9424544975778915
118	49	-1.5342548442286166
118	50	0.8546900623785234
118	51	4.046767665549357
118	52	4.365497120931565
118	53	3.6303748775341163
118	54	4.193838981339983
118	55	5.308655207840189
118	56	7.513532526747863
119	1	4.073253300297111
119	2	4.106603357279127
119	3	3.8399774588440394
119	4	6.897049844682165
119	5	7.430125519768058
119	6	4.192302629237723
119	7	3.883727491872328
119	8	3.435506709616405
119	9	4.283126575519859
119	10	4.5729538091057975
119	11	1.264625036262217
119	12	-5.314826835060533
119	13	-1.751690113710513
119	14	-3.5632889131981464
119	15	-6.54523020896244
119	16	-8.61081756893892
119	17	-0.012292116561955169
119	18	-7.660182400479908
119	19	-0.0826868834229586
119	20	-0.8569490349657798
119	21	3.7997807285568506
119	22	4.717304734005406
119	23	4.1156222642466
119	24	3.5030031661313252
119	25	4.374408084382946
119	26	5.92548045365109
119	27	5.318343214975741
119	28	5.171657026362759
119	29	5.184086291349363
119	30	5.281109538248895
119	31	3.6655903805931196
119	32	3.471741544765461
119	33	4.297226490143881
119	34	4.201158293096963
119	35	4.430319123033794
119	36	4.239635229890755
119	37	4.984189786688301
119	38	5.324564992506755
119	39	6.607101324462757
119	40	8.33147561699258
119	41	6.821456991284162
119	42	6.156557387743899
119	43	-4.226608112791794
119	44	-2.3318141655419673
119	45	4.922037838194406
119	46	-0.47124451913099463
119	47	-3.1757542834705106
119	48	-7.362367917491312
119	49	-2.190099000072774
119	50	0.6404043480928081
119	51	5.174473293254984
119	52	6.592769848204291
119	53	6.050288297447537
119	54	5.15920694670795
119	55	6.966663865848847
119	56	9.340372353587693
120	1	4.082777109820924
120	2	3.9661271668029343
120	3	3.7149774588440394
120	4	7.216097463729788
120	5	7.501554091196628
120	6	4.326826438761536
120	7	3.9456322537770845
120	8	3.9712209953306896
120	9	4.575983718377003
120	10	4.696763332915324
120	11	-0.47823210659493043
120	12	-8.596969692203391
120	13	-9.863594875615277
120	14	-5.435907960817193
120	15	-1.099992113724344
120	16	-7.502484235605589
120	17	-0.12776830703814568
120	18	-5.49351573381324
120	19	-0.18506783580390973
120	20	-1.6593299873467338
120	21	3.1045426333187542
120	22	4.3553999721006456
120	23	3.885860359484692
120	24	4.304193642321803
120	25	3.867265227240093
120	26	4.033813786984425
120	27	4.442152738785268
120	28	4.510942740648474
120	29	4.87337200563508
120	30	4.87634763348699
120	31	4.879876094878835
120	32	4.325312973336889
120	33	4.672226490143881
120	34	4.4356821026207705
120	35	3.3993667420814155
120	36	5.012254277509808
120	37	5.374665977164494
120	38	5.340041182982947
120	39	6.467815610177045
120	40	7.102904188421153
120	41	-2.7225906277634593
120	42	-0.3541568979703875
120	43	-6.6277985889822695
120	44	-14.606814165541968
120	45	-3.2291526379960693
120	46	-6.614101661988137
120	47	-5.6745638072800375
120	48	-3.3576060127294056
120	49	-14.419860904834678
120	50	-2.086976604288143
120	51	3.964949483731175
120	52	3.267769848204292
120	53	4.203859726018965
120	54	3.9627783752793775
120	55	3.6071400563250364
120	56	7.841562829778166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1093.0
5	25.0
6	63.0
7	77.0
8	144.0
9	192.0
10	139.0
11	84.0
12	104.0
13	87.0
14	121.0
15	154.0
16	192.0
17	255.0
18	321.0
19	422.0
20	561.0
21	708.0
22	853.0
23	1005.0
24	1118.0
25	1225.0
26	1384.0
27	1426.0
28	1522.0
29	1677.0
30	1818.0
31	2014.0
32	2275.0
33	2545.0
34	2770.0
35	3041.0
36	3381.0
37	3678.0
38	3612.0
39	3104.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.63881364173092	0.3079345233960779	0.03936005186265657	0.013891783010349379
8	99.73142552846657	0.15744020745062628	0.05093653770461439	0.06019772637818063
9	99.09932854827507	0.2570039360963186	0.26858068997453116	0.3750868256540866
10	52.21458174156653	23.606769928920375	16.6029959945359	7.575652334977194
11	34.01171540367206	30.508670787895625	18.300108818966915	17.179504989465396
12	28.901854553031882	18.733069389456137	19.80505197842143	32.560024079090546
13	23.484059178995626	15.34578963209928	23.01868444814892	38.15146674075617
14	21.89576532147901	22.032367854414115	32.340070848093355	23.73179597601352
15	34.3467944542663	17.807295059086258	23.550758351167765	24.29515213547967
16	35.54237798522156	22.725903963308703	21.285121956869194	20.44659609460054
17	26.727811747267	19.10320548452844	34.357050213081344	19.811932555123217
18	22.657498089879834	19.74485425204325	31.02498205644694	26.572665601629968
19	32.237070662621306	21.455867707344186	21.330801120967184	24.976260509067327
20	24.175977006165684	30.82147327430346	24.525983959946224	20.476565759584627
21	23.93146561704098	21.05811530446863	30.81268812225052	24.197730956239873
22	22.99090088212822	20.56215415248547	25.871130559607323	30.575814405778985
23	22.655182792711447	20.86777337871316	24.86629158852539	31.61075224005001
24	22.35215116009818	21.821886722548975	29.041819108044276	26.78414300930857
25	32.15244975456145	22.13809391497638	23.33981661572659	22.369639714735577
26	27.17922583335264	21.598832495540783	20.030577497741433	31.191364173365145
27	22.022237665045168	23.73407458883484	20.889506601806808	33.35418114431318
28	25.262207404320346	31.286610636475192	20.29126438378366	23.159917575420806
29	35.8032133169499	23.265249345049035	19.764449493427307	21.16708784457376
30	26.85243091747157	20.60778727445394	31.62161536145276	20.91816644662173
31	27.126021624875552	30.855965363154358	21.986061911046285	20.031951100923802
32	36.7402227522171	22.47898673211846	20.026859934702575	20.753930580961864
33	27.68531825135599	22.595150897037687	20.868295396597283	28.85123545500904
34	23.642646751861214	30.329104529535915	26.14745923881532	19.880789479787556
35	22.140306950433533	24.19900774331154	34.44846292947559	19.21222237677934
36	25.060804706863404	32.08867063538023	22.105116860855667	20.745407796900697
37	25.414377448594017	22.878271553422817	31.372139926281385	20.335211071701785
38	34.92531081833364	23.285860085359065	21.63202820560401	20.156800890703288
39	26.131410553649264	22.82296206992488	20.015301864045256	31.030325512380596
40	21.4564217506012	18.526301043636618	27.283976559034347	32.733300646727834
41	18.532728208487345	16.25181303513779	25.064333504889348	40.15112525148552
42	28.297768593122345	24.15074186732301	22.158497225253672	25.392992314300972
43	20.37527286238447	19.543913427151548	31.74028145464679	28.34053225581719
44	21.372945579654555	21.891345282097777	23.46979101285538	33.26591812539228
45	24.33491824191117	21.84622521164328	31.453090571726776	22.365765974718776
46	21.201774556941444	24.683529603047408	22.43746080412515	31.677235035886003
47	18.86831945671892	27.178008279454858	22.74756965440253	31.20610260942369
48	23.703412012693708	28.778578212225796	24.145838641680758	23.372171133399736
49	20.492050597655798	28.385749100615065	22.573981664152257	28.548218637576884
50	28.9706189272546	22.790622173781973	24.67128910326276	23.56746979570067
51	21.761441474911187	30.111222049364944	25.68323774583788	22.44409872988599
52	22.5038847786256	24.765173829348054	31.182596191757312	21.548345200269036
53	21.686830922137336	23.256624015976595	32.65448296681606	22.402062095070015
54	21.39673105497771	29.3694279346211	25.545598068350667	23.68824294205052
55	21.156882779119453	29.309864387887796	25.274010774660972	24.259242058331782
56	28.316077812340406	22.066948326291843	26.180416918148474	23.436556943219276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	2.0
10	25.5
11	49.0
12	78.0
13	119.5
14	132.0
15	119.0
16	106.0
17	115.0
18	124.0
19	116.5
20	109.0
21	107.0
22	105.5
23	106.0
24	112.5
25	119.0
26	120.0
27	121.0
28	153.5
29	200.5
30	215.0
31	270.5
32	326.0
33	364.5
34	403.0
35	473.5
36	544.0
37	602.0
38	679.5
39	699.0
40	766.5
41	834.0
42	935.5
43	1037.0
44	1361.5
45	1686.0
46	2745.0
47	3581.5
48	3359.0
49	2852.5
50	2346.0
51	2260.0
52	2174.0
53	2276.0
54	2461.5
55	2545.0
56	2506.5
57	2468.0
58	2560.0
59	2652.0
60	2870.5
61	3089.0
62	2866.5
63	2363.0
64	2082.0
65	1955.5
66	1829.0
67	1562.5
68	1296.0
69	1150.0
70	1004.0
71	848.0
72	605.5
73	519.0
74	407.0
75	295.0
76	208.5
77	122.0
78	104.5
79	91.5
80	96.0
81	62.5
82	29.0
83	18.5
84	8.0
85	5.0
86	2.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002315297168391563
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4700053251834873
16	0.04630594336783126
17	0.03472945752587345
18	0.0
19	0.03241416035748188
20	0.1134495612511866
21	0.002315297168391563
22	0.0
23	0.0
24	0.011576485841957815
25	0.0069458915051746895
26	0.05093653770461439
27	0.048621240536222826
28	0.0
29	0.13428723576671067
30	0.04167534903104814
31	0.0
32	0.009261188673566251
33	0.1134495612511866
34	0.17133199046097566
35	0.1319719385983191
36	0.04630594336783126
37	0.1250260470931444
38	0.18290847630293347
39	0.13660253293510222
40	0.8335069806209627
41	1.0303072399342454
42	0.2870968488805538
43	0.2986733347225116
44	0.40286170730013193
45	0.1759625847977588
46	0.3195110092380357
47	0.44685235349957164
48	0.04630594336783126
49	0.24542149984950568
50	0.15744020745062628
51	0.2870968488805538
52	0.17133199046097566
53	0.29635803755412005
54	0.275520363038596
55	0.29404274038572853
56	0.2616285800282466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	43191.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.57278136648839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52262802057386	63.358107013035124
2	6.773849189661307	9.696464541223866
3	1.924756574903762	4.13280544557894
4	0.9963445799501827	2.8524461114584057
5	0.4334745899783263	1.5512491028223472
6	0.30407918998479605	1.3058276029728415
7	0.2199721799890014	1.102081452154384
8	0.12616051499369196	0.7223727165381676
9	0.08410700999579465	0.5417795374036257
>10	0.5628699899718566	6.850964321270634
>50	0.029113964998544302	1.5906091546850039
>100	0.019409309999029532	2.9311662151837186
>500	0.0	0.0
>1k	0.0032348849998382555	3.364126785672941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1453	3.364126785672941	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	397	0.9191729758514505	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	213	0.4931582968674029	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	206	0.476951216688662	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	177	0.4098075988053067	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	154	0.3565557639323007	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	119	0.275520363038596	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	99	0.22921441967076475	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	98	0.2268991225023732	No Hit
GCGAATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	97	0.2245838253339816	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	83	0.19216966497649973	No Hit
GCGAATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	71	0.16438609895580097	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	69	0.15975550461901786	No Hit
GCGAATGGGAGTGCTGCGAATGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTCTC	60	0.1389178301034938	No Hit
GCGAATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	56	0.12965664142992753	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	54	0.1250260470931444	No Hit
GCGAATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	46	0.1065036697460119	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	45	0.10418837257762034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004630594336783126	0.0	0.0	0.0	0.0
12	0.0069458915051746895	0.0	0.0	0.0	0.0
13	0.009261188673566251	0.0	0.0	0.0	0.0
14	0.018522377347132503	0.0	0.0	0.0	0.0
15	0.018522377347132503	0.0	0.0	0.0	0.0
16	0.02315297168391563	0.0	0.0	0.0	0.0
17	0.025468268852307192	0.0	0.0	0.0	0.0
18	0.03009886318909032	0.0	0.0	0.0	0.0
19	0.03009886318909032	0.0	0.0	0.0	0.0
20	0.03241416035748188	0.0	0.0	0.0	0.0
21	0.03241416035748188	0.0	0.0	0.0	0.0
22	0.03241416035748188	0.0	0.0	0.0	0.0
23	0.03472945752587345	0.0	0.0	0.0	0.0
24	0.037044754694265006	0.0	0.0	0.0	0.0
25	0.03936005186265657	0.0	0.0	0.0	0.0
26	0.04167534903104814	0.0	0.0	0.0	0.0
27	0.050936537704614385	0.0	0.0	0.0	0.0
28	0.06019772637818064	0.0	0.0	0.0	0.0
29	0.0625130235465722	0.0	0.0	0.0	0.0
30	0.0625130235465722	0.0	0.0	0.0	0.0
31	0.07177421222013845	0.0	0.0	0.0	0.0
32	0.07408950938853001	0.0	0.0	0.0	0.0
33	0.07408950938853001	0.0	0.0	0.0	0.0
34	0.07408950938853001	0.0	0.0	0.0	0.0
35	0.07408950938853001	0.0	0.0	0.0	0.0
36	0.07408950938853001	0.0	0.0	0.0	0.0
37	0.07408950938853001	0.0	0.0	0.0	0.0
38	0.07640480655692158	0.0	0.0	0.002315297168391563	0.0
39	0.07640480655692158	0.0	0.0	0.002315297168391563	0.0
40	0.07872010372531314	0.0	0.0	0.002315297168391563	0.0
41	0.07872010372531314	0.0	0.0	0.002315297168391563	0.0
42	0.08335069806209627	0.0	0.0	0.002315297168391563	0.0
43	0.08798129239887939	0.0	0.0	0.002315297168391563	0.0
44	0.09261188673566252	0.0	0.0	0.002315297168391563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATTC	15	0.007926756	50.701298	41
CGTCTCA	40	2.2791937E-9	50.701298	41
AGCCCCC	15	0.007963879	50.64151	38
CCAACGG	15	0.007963879	50.64151	40
TCGTCTC	35	4.6389687E-8	50.641506	40
TATGCCC	15	0.008038514	50.522354	35
TCTCGTG	15	0.008038514	50.522354	35
ATGCCTG	25	1.9309704E-5	50.52235	36
TATGCTC	35	4.72628E-8	50.522346	35
ATGCTCG	35	4.72628E-8	50.522346	36
GTCTCGT	15	0.008113667	50.403755	34
CACGATT	20	4.070533E-4	50.16822	47
CAATTCG	15	0.008303839	50.109688	42
ATTGCTC	15	0.008303839	50.109688	46
ACAGTCT	15	0.008342267	50.05128	14
CAGTCTT	15	0.008342267	50.05128	15
AATTCGC	15	0.008342267	50.05128	43
GTAGTCG	15	0.008342267	50.05128	14
TGGTAGT	15	0.0083808275	49.993015	12
ATGGTAG	15	0.0083808275	49.993015	11
>>END_MODULE