FastQCFastQC Report
Fri 17 Jun 2016
SRR1525957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC130.31408552790529115No Hit
GCGAATGGGGTTTCAATGGCTTTCTTCCCTTGACCTTTTT120.2899251026818072No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.24160425223483933No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT90.2174438270113554No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.2174438270113554No Hit
GCGAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT80.19328340178787146No Hit
GCGAATGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC70.16912297656438754No Hit
GCGAATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT60.1449625513409036No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC60.1449625513409036No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.12080212611741967No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC50.12080212611741967No Hit
GCGAATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT50.12080212611741967No Hit
GCGAATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTT50.12080212611741967No Hit
GCGAATGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.12080212611741967No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT50.12080212611741967No Hit
GCGAATGGGGAAATGAAGGCCTAAGTCCAGCTGGTTTTCA50.12080212611741967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT301.07806045E-534.3333365
ATGGGAG502.055458E-1034.3333365
TGGGGGG502.055458E-1034.3333366
TGGGAGA251.6000302E-434.3333366
ATGGGTG200.002359320234.3333325
ATGGGGC200.002359320234.3333325
ATGGGGA404.7850335E-834.3333325
ATGGGAC200.002359320234.3333325
TGGGGGA200.002359320234.3333326
AATGGGG1650.034.3333324
TGGGTAC200.002359320234.3333326
TGGGAAC200.002359320234.3333326
GCGAATG4150.033.5060231
GAATGGG4150.033.5060233
CGAATGG4150.033.5060232
AATGGGA1500.033.188894
ATGGGGG950.032.5263145
AATGGGT651.8189894E-1231.6923074
ATGGGAA508.816642E-930.9000025
AATGGGC402.0503558E-630.0416664
Produced by FastQC (version 0.11.5)