##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4139
Sequences flagged as poor quality	0
Sequence length	40
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.683740033824595	29.0	22.0	30.0	4.0	33.0
2	11.73689296931626	11.0	9.0	11.0	4.0	22.0
3	18.588064749939598	20.0	12.0	25.0	4.0	30.0
4	19.195457840057983	20.0	10.0	27.0	4.0	32.0
5	18.976322783280985	21.0	11.0	26.0	4.0	30.0
6	22.596037690263348	26.0	17.0	30.0	4.0	33.0
7	21.48079246194733	25.0	14.0	30.0	4.0	33.0
8	22.17178062333897	26.0	15.0	30.0	4.0	33.0
9	20.698236288958686	24.0	11.0	29.0	4.0	32.0
10	18.448659096400096	19.0	9.0	27.0	4.0	32.0
11	23.503986470161873	28.0	12.0	35.0	4.0	38.0
12	22.887412418458563	27.0	11.0	34.0	4.0	37.0
13	23.62599661754047	28.0	11.0	35.0	4.0	37.0
14	24.06837400338246	30.0	10.0	35.0	4.0	38.0
15	22.410968833051463	26.0	4.0	35.0	4.0	37.0
16	20.54167673351051	24.0	4.0	33.0	4.0	36.0
17	20.93114278811307	26.0	4.0	33.0	4.0	36.0
18	20.660304421357814	25.0	4.0	33.0	4.0	36.0
19	17.81783039381493	18.0	4.0	30.0	4.0	35.0
20	19.40879439478135	22.0	4.0	32.0	4.0	36.0
21	16.76636868808891	14.0	4.0	30.0	4.0	35.0
22	16.7219135056777	14.0	4.0	30.0	4.0	35.0
23	16.79028750906016	15.0	4.0	30.0	4.0	35.0
24	15.879439478134815	10.0	4.0	30.0	4.0	35.0
25	15.250060401063058	4.0	4.0	29.0	4.0	34.0
26	12.355158250785214	4.0	4.0	23.0	4.0	32.0
27	12.259482966900217	4.0	4.0	23.0	4.0	32.0
28	11.82024643633728	4.0	4.0	22.0	4.0	32.0
29	11.467021019569945	4.0	4.0	20.0	4.0	32.0
30	10.517757912539262	4.0	4.0	16.0	4.0	31.0
31	10.221067890794878	4.0	4.0	14.0	4.0	31.0
32	9.696545059193042	4.0	4.0	10.0	4.0	30.0
33	9.320850446967867	4.0	4.0	4.0	4.0	30.0
34	9.081420633003141	4.0	4.0	4.0	4.0	29.0
35	8.607393090118386	4.0	4.0	4.0	4.0	28.0
36	8.57163566078763	4.0	4.0	4.0	4.0	30.0
37	8.359990335829911	4.0	4.0	4.0	4.0	29.0
38	8.275428847547717	4.0	4.0	4.0	4.0	29.0
39	8.050495288717082	4.0	4.0	4.0	4.0	28.0
40	8.006523314810341	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	11.308522460445541
1	2	22.418598708983325
1	3	6.420627510462673
1	4	16.888641486718413
1	5	15.792505479868119
1	6	14.20429573479024
1	7	14.178704110297513
1	8	5.828289872520635
1	9	4.997308338791861
1	10	11.993327185634875
1	11	2.1618941193117003
1	12	1.966573810804583
1	13	-7.743723462130053
1	14	-0.8777854319063039
1	15	2.1885482344273512
1	16	0.6173720967676992
1	17	-2.706208718021905
1	18	-1.5999529469309621
1	19	-2.429291861984172
1	20	2.0240192957225958
1	21	6.575625046504166
1	22	5.2264976476514935
1	23	4.12014228140602
1	24	4.189597063498162
1	25	1.8671497306112688
1	26	3.4943474413254645
1	27	4.37406857489275
1	28	4.630829122037909
1	29	5.428121024274869
1	30	1.705865502019348
1	31	3.2533121335319137
1	32	3.838520240443314
1	33	0.8639007512633867
1	34	2.5735650491144995
1	35	-0.08291098400988517
1	36	-0.6698500827621698
1	37	0.3476550616660514
1	38	0.04535226678084037
1	39	2.3683729274388616
1	40	1.1608494374977871
4	1	-19.15301600109292
4	2	-6.88909359870898
4	3	-13.887064797229634
4	4	-13.572896974820047
4	5	-13.207494520131881
4	6	-16.488011957517454
4	7	-16.282834351240947
4	8	-16.402479358248595
4	9	-15.77192243043891
4	10	-12.622057429749741
4	11	-18.145798188380606
4	12	-17.571887727656957
4	13	-18.512954231360823
4	14	-18.416246970367844
4	15	-17.27299022711111
4	16	-15.15185867246307
4	17	-15.860054871868059
4	18	-15.599952946930962
4	19	-12.583138015830325
4	20	-13.514442242738944
4	21	-10.193605722726604
4	22	-9.69657927542543
4	23	-10.187550026286287
4	24	-9.502710628809531
4	25	-9.286696423234885
4	26	-7.7364217894437655
4	27	-7.318239117414942
4	28	-7.061478570269783
4	29	-6.6488020526482075
4	30	-6.140288344134499
4	31	-6.208226328006548
4	32	-5.8537874518643775
4	33	-5.21302232565969
4	34	-5.349511873962424
4	35	-4.929064830163732
4	36	-4.439080851992939
4	37	-4.34465263064164
4	38	-4.262340040911468
4	39	-3.9393193802534467
4	40	-3.454535177886828
6	1	2.5969839989070813
6	2	0.3609064012910199
6	3	0.11293520277036606
6	4	-4.572896974820047
6	5	-3.707494520131881
6	6	5.761988042482546
6	7	-0.2828343512409468
6	8	-2.152479358248595
6	9	-0.5219224304389094
6	10	0.12794257025025857
6	11	1.8542018116193937
6	12	7.178112272343043
6	13	2.7370457686391774
6	14	5.083753029632156
6	15	4.727009772888891
6	16	1.8481413275369292
6	17	1.8899451281319415
6	18	4.400047053069038
6	19	8.416861984169675
6	20	0.48555775726105566
6	21	10.556394277273396
6	22	14.80342072457457
6	23	12.562449973713713
6	24	10.997289371190469
6	25	13.463303576765115
6	26	17.013578210556233
6	27	14.681760882585058
6	28	21.188521429730216
6	29	16.351197947351793
6	30	16.359711655865503
6	31	18.541773671993454
6	32	9.396212548135622
6	33	14.28697767434031
6	34	15.900488126037576
6	35	20.820935169836268
6	36	13.560919148007061
6	37	14.15534736935836
6	38	17.987659959088532
6	39	6.060680619746553
6	40	6.545464822113172
7	1	2.4719839989070813
7	2	0.4859064012910199
7	3	4.987935202770366
7	4	3.302103025179953
7	5	0.2925054798681188
7	6	2.386988042482546
7	7	-0.6578343512409468
7	8	-2.777479358248595
7	9	-0.1469224304389094
7	10	9.377942570250259
7	11	5.979201811619394
7	12	4.303112272343043
7	13	7.112045768639177
7	14	5.458753029632156
7	15	8.352009772888891
7	16	-0.7768586724630708
7	17	4.7649451281319415
7	18	-0.7249529469309621
7	19	2.0418619841696746
7	20	8.110557757261056
7	21	6.056394277273396
7	22	11.17842072457457
7	23	8.187449973713713
7	24	7.622289371190469
7	25	10.838303576765115
7	26	5.3885782105562345
7	27	5.556760882585058
7	28	7.938521429730217
7	29	7.3511979473517925
7	30	7.859711655865501
7	31	8.041773671993452
7	32	5.5212125481356225
7	33	2.78697767434031
7	34	3.025488126037576
7	35	-0.17906483016373187
7	36	2.435919148007061
7	37	1.2803473693583598
7	38	2.237659959088532
7	39	1.1856806197465533
7	40	2.920464822113172
9	1	6.489841141764224
9	2	8.325192115576735
9	3	6.041506631341793
9	4	9.35567445375138
9	5	3.935362622725261
9	6	9.511988042482546
9	7	9.717165648759053
9	8	9.31180635603712
9	9	9.513791855275375
9	10	10.663656855964543
9	11	6.997058954476536
9	12	8.85668370091447
9	13	10.201331482924893
9	14	5.726610172489298
9	15	6.369866915746034
9	16	9.705284184679787
9	17	9.4970879852748
9	18	10.257189910211896
9	19	10.13114769845539
9	20	11.842700614403913
9	21	17.949251420130544
9	22	15.017706438860285
9	23	13.883878545142286
9	24	17.425860799761896
9	25	16.213303576765114
9	26	2.1921496391276634
9	27	0.9674751682993445
9	28	0.009950001158788524
9	29	0.06548366163750607
9	30	0.07399737015121488
9	31	0.9346308148505944
9	32	-0.28235888043580637
9	33	1.3584062457688812
9	34	-1.1352261596767104
9	35	2.070935169836268
9	36	3.8466334337213475
9	37	4.15534736935836
9	38	1.0948028162313896
9	39	2.8463949054608397
9	40	1.474036250684601
10	1	1.7136506655737485
10	2	0.04423973462435349
10	3	2.8462685361037003
10	4	7.960436358513288
10	5	1.7925054798681188
10	6	3.511988042482546
10	7	2.6504989820923868
10	8	4.3308539750847395
10	9	0.6947442362277556
10	10	6.777942570250257
10	11	5.120868478286059
10	12	11.22811227234304
10	13	4.887045768639176
10	14	8.450419696298823
10	15	4.660343106222225
10	16	11.381474660870264
10	17	12.60661179479861
10	18	9.666713719735704
10	19	12.483528650836341
10	20	10.41889109059439
10	21	10.406394277273398
10	22	16.236754057907902
10	23	15.145783307047045
10	24	13.497289371190469
10	25	15.44663691009845
10	26	11.79691154388957
10	27	11.481760882585059
10	28	9.205188096396883
10	29	10.084531280685127
10	30	4.326378322532168
10	31	4.791773671993452
10	32	2.4795458814689564
10	33	3.3203110076736433
10	34	4.850488126037575
10	35	1.1376018365029346
10	36	2.9609191480070614
10	37	2.7886807026916927
10	38	3.5376599590885327
10	39	2.0606806197465533
10	40	2.0787981554465054
12	1	8.51365066557375
12	2	-0.222426932042314
12	3	9.112935202770366
12	4	6.093769691846621
12	5	2.792505479868119
12	6	3.178654709149214
12	7	1.0504989820923853
12	8	2.264187308418073
12	9	0.2280775695610906
12	10	2.0446092369169264
12	11	7.5208684782860615
12	12	4.428112272343043
12	13	4.8203791019725095
12	14	5.917086362965488
12	15	5.0603431062222235
12	16	4.514807994203597
12	17	8.473278461465274
12	18	4.400047053069038
12	19	7.750195317503007
12	20	12.485557757261056
12	21	4.806394277273396
12	22	2.6367540579079023
12	23	2.1457833070470453
12	24	5.497289371190469
12	25	5.046636910098448
12	26	0.5969115438895685
12	27	11.681760882585058
12	28	12.938521429730217
12	29	10.351197947351793
12	30	6.1930449891988335
12	31	10.125107005326784
12	32	1.1462125481356225
12	33	13.78697767434031
12	34	7.317154792704244
12	35	12.070935169836268
12	36	5.894252481340395
12	37	5.988680702691694
12	38	6.070993292421866
12	39	6.394013953079887
12	40	6.878798155446506
13	1	-0.6530160010929187
13	2	-1.0557602653756462
13	3	0.11293520277036606
13	4	-4.239563641486713
13	5	-3.540827853465215
13	6	1.011988042482546
13	7	-0.2828343512409468
13	8	-0.06914602491526267
13	9	-1.4385890971055773
13	10	-1.6220574297497414
13	11	6.354201811619394
13	12	-1.738554394323625
13	13	5.3203791019725095
13	14	4.417086362965488
13	15	5.0603431062222235
13	16	9.34814132753693
13	17	7.473278461465274
13	18	7.900047053069038
13	19	9.416861984169675
13	20	9.985557757261056
13	21	13.473060943940064
13	22	15.136754057907902
13	23	14.979116640380381
13	24	11.997289371190469
13	25	14.713303576765115
13	26	4.930244877222902
13	27	1.3484275492517241
13	28	-1.2281452369364487
13	29	3.1845312806851265
13	30	3.6930449891988353
13	31	-1.874892994673214
13	32	-5.8537874518643775
13	33	-5.21302232565969
13	34	-5.349511873962424
13	35	-4.929064830163732
13	36	-4.439080851992939
13	37	-4.34465263064164
13	38	-4.262340040911468
13	39	-3.9393193802534467
13	40	-3.454535177886828
15	1	8.680317332240413
15	2	4.277573067957686
15	3	10.612935202770366
15	4	6.760436358513285
15	5	12.459172146534787
15	6	8.511988042482546
15	7	6.717165648759053
15	8	5.764187308418073
15	9	6.228077569561091
15	10	12.544609236916926
15	11	5.5208684782860615
15	12	6.928112272343043
15	13	6.653712435305845
15	14	6.417086362965488
15	15	6.0603431062222235
15	16	9.84814132753693
15	17	8.139945128131941
15	18	11.73338038640237
15	19	9.583528650836342
15	20	10.485557757261056
15	21	14.639727610606728
15	22	13.80342072457457
15	23	16.479116640380383
15	24	19.330622704523805
15	25	14.713303576765115
15	26	5.596911543889567
15	27	3.015094215918392
15	28	-1.8948119036031166
15	29	-0.14880205264820745
15	30	1.8597116558655014
15	31	0.9584403386601181
15	32	-1.3537874518643775
15	33	1.9536443410069761
15	34	-2.349511873962424
15	35	-3.262398163497065
15	36	4.227585814673727
15	37	2.822014036025026
15	38	1.237659959088532
15	39	1.3940139530798872
15	40	5.045464822113172
16	1	9.275555427478512
16	2	5.753763544148162
16	3	10.89864948848465
16	4	12.569960168037095
16	5	7.721076908439546
16	6	9.869130899625404
16	7	5.288594220187626
16	8	6.88323492746569
16	9	7.156648998132518
16	10	11.020799713107401
16	11	5.568487525905109
16	12	6.85668370091447
16	13	7.272760054353462
16	14	9.36946731534644
16	15	8.655581201460318
16	16	5.4909984703940715
16	17	7.782802270989084
16	18	7.900047053069038
16	19	8.488290555598248
16	20	9.128414900118198
16	21	3.8063942772733963
16	22	7.30342072457457
16	23	6.4553071165708555
16	24	7.568717942619042
16	25	1.1418750053365443
16	26	-0.3792789323009078
16	27	0.7531894540136292
16	28	-1.4186214274126403
16	29	-1.363087766933921
16	30	0.07399737015121488
16	31	-1.9939406137208344
16	32	0.5747839767070513
16	33	-0.9273080399454035
16	34	-1.2780833025338527
16	35	-1.7862076873065886
16	36	-1.7247951377072246
16	37	-0.058938344927353725
16	38	-2.262340040911468
16	39	-1.7250336659677323
16	40	-1.2402494636011134
17	1	5.937893089816171
17	2	2.7472700376546566
17	3	7.658389748224913
17	4	7.699830297907226
17	5	6.974323661686302
17	6	6.875624406118909
17	7	6.44443837603178
17	8	7.779338823569589
17	9	5.864441205924727
17	10	7.741578933886622
17	11	5.217838175255757
17	12	6.337203181433953
17	13	6.305227586820994
17	14	8.492843938723066
17	15	6.999737045616165
17	16	9.84814132753693
17	17	9.230854219041031
17	18	12.763683416705405
17	19	14.780498347806038
17	20	9.303739575442872
17	21	10.624576095455213
17	22	10.939784360938207
17	23	11.63063179189553
17	24	11.406380280281379
17	25	12.167849031310569
17	26	6.3544873014653245
17	27	12.045397246221421
17	28	9.665794157002944
17	29	11.714834310988156
17	30	10.859711655865501
17	31	11.791773671993452
17	32	9.146212548135622
17	33	7.605159492522127
17	34	2.5595790351284844
17	35	0.5254806243817232
17	36	3.1972827843706977
17	37	1.4735291875401781
17	38	2.5558417772703503
17	39	1.242498801564735
17	40	3.090919367567717
18	1	6.616214768137851
18	2	-0.35063206024744176
18	3	4.728319818154983
18	4	3.7347953328722596
18	5	8.561736249098889
18	6	8.050449580944086
18	7	5.33255026414367
18	8	9.674443718674482
18	9	4.151154492638014
18	10	5.8394810317887185
18	11	9.008047965465547
18	12	6.197343041573813
18	13	7.794738076331484
18	14	8.122214568093696
18	15	6.342394388273508
18	16	6.463525942921546
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103	1	-6.653016001092919
103	2	-3.555760265375647
103	3	-5.5537314638963
103	4	-6.072896974820047
103	5	-5.374161186798547
103	6	-7.488011957517454
103	7	-4.116167684574279
103	8	-12.235812691581929
103	9	-5.271922430438909
103	10	-6.7887240964164075
103	11	-2.6457981883806063
103	12	-1.9052210609902893
103	13	-3.0129542313608226
103	14	-2.749580303701176
103	15	-5.772990227111109
103	16	-0.15185867246307083
103	17	-1.6933882052013907
103	18	-0.76661961359763
103	19	-2.2498046824969915
103	20	-4.014442242738944
103	21	-10.193605722726604
103	22	-9.69657927542543
103	23	-10.187550026286287
103	24	-9.502710628809531
103	25	-9.286696423234885
103	26	-7.7364217894437655
103	27	-7.318239117414942
103	28	-7.061478570269783
103	29	-6.6488020526482075
103	30	-6.140288344134499
103	31	-6.208226328006548
103	32	-5.8537874518643775
103	33	-5.21302232565969
103	34	-5.349511873962424
103	35	-4.929064830163732
103	36	-4.439080851992939
103	37	-4.34465263064164
103	38	-4.262340040911468
103	39	-3.9393193802534467
103	40	-3.454535177886828
104	1	-0.9863493344262508
104	2	-1.38909359870898
104	3	0.4462685361036982
104	4	-0.23956364148671483
104	5	0.45917214653478666
104	6	-1.1546786241841218
104	7	-3.616167684574279
104	8	-4.735812691581929
104	9	-3.2719224304389094
104	10	-3.9553907630830754
104	11	-3.812464855047274
104	12	0.761445605676375
104	13	-1.3462875646941548
104	14	-2.416246970367844
104	15	-2.106323560444441
104	16	-2.151858672463071
104	17	2.4732784614652736
104	18	-4.933286280264296
104	19	2.5835286508363424
104	20	-2.8477755760722783
104	21	-2.8602723893932698
104	22	-6.029912608758763
104	23	-2.0208833596196207
104	24	-4.669377295476197
104	25	-4.453363089901551
104	26	1.9302448772229006
104	27	-0.8182391174149419
104	28	-0.5614785702697827
104	29	3.1845312806851265
104	30	1.6930449891988353
104	31	-4.041559661339881
104	32	-1.3537874518643775
104	33	-4.046355658993023
104	34	-0.51617854062909
104	35	-4.929064830163732
104	36	-4.439080851992939
104	37	-4.34465263064164
104	38	-4.262340040911468
104	39	-3.9393193802534467
104	40	-3.454535177886828
105	1	1.3469839989070813
105	2	-0.6390935987089801
105	3	2.862935202770366
105	4	4.427103025179953
105	5	2.042505479868119
105	6	3.011988042482546
105	7	1.7171656487590532
105	8	-1.1524793582485948
105	9	3.7280775695610906
105	10	0.6279425702502586
105	11	13.854201811619394
105	12	5.178112272343043
105	13	7.487045768639177
105	14	6.833753029632156
105	15	8.227009772888891
105	16	12.09814132753693
105	17	9.889945128131941
105	18	-1.0999529469309621
105	19	4.916861984169675
105	20	-0.5144422427389443
105	21	3.5563942772733963
105	22	1.5534207245745701
105	23	2.312449973713713
105	24	3.997289371190469
105	25	1.9633035767651155
105	26	6.5135782105562345
105	27	5.931760882585058
105	28	7.688521429730217
105	29	5.6011979473517925
105	30	3.8597116558655014
105	31	5.041773671993452
105	32	7.3962125481356225
105	33	4.53697767434031
105	34	9.900488126037576
105	35	2.570935169836268
105	36	0.560919148007061
105	37	2.65534736935836
105	38	0.737659959088532
105	39	4.310680619746553
105	40	-0.20453517788682785
106	1	-3.8196826677595865
106	2	-2.722426932042314
106	3	-4.0537314638963
106	4	-3.239563641486713
106	5	-3.207494520131881
106	6	-0.48801195751745396
106	7	-4.282834351240947
106	8	-0.4024793582485948
106	9	-0.7719224304389094
106	10	-1.2887240964164075
106	11	1.8542018116193937
106	12	3.5947789390097107
106	13	2.653712435305845
106	14	2.917086362965488
106	15	2.393676439555559
106	16	-2.651858672463071
106	17	-5.8600548718680585
106	18	-2.76661961359763
106	19	-10.249804682496993
106	20	-7.847775576072278
106	21	-5.693605722726604
106	22	-8.19657927542543
106	23	-5.687550026286287
106	24	-9.502710628809531
106	25	-9.286696423234885
106	26	-7.7364217894437655
106	27	-7.318239117414942
106	28	-7.061478570269783
106	29	-6.6488020526482075
106	30	-6.140288344134499
106	31	-6.208226328006548
106	32	-5.8537874518643775
106	33	-5.21302232565969
106	34	-5.349511873962424
106	35	-4.929064830163732
106	36	-4.439080851992939
106	37	-4.34465263064164
106	38	-4.262340040911468
106	39	-3.9393193802534467
106	40	-3.454535177886828
107	1	1.5136506655737492
107	2	-2.055760265375646
107	3	0.7796018694370339
107	4	-0.07289697482004698
107	5	2.9591721465347867
107	6	3.345321375815878
107	7	5.050498982092385
107	8	-0.9024793582485948
107	9	6.8947442362277584
107	10	7.544609236916926
107	11	-1.9791315217139385
107	12	4.261445605676375
107	13	-0.17962089802749048
107	14	5.250419696298824
107	15	-9.772990227111109
107	16	-5.151858672463071
107	17	-9.026721538534725
107	18	-12.433286280264294
107	19	-11.249804682496993
107	20	-9.014442242738944
107	21	-7.360272389393271
107	22	-4.863245942092096
107	23	-5.354216692952953
107	24	-4.669377295476197
107	25	-5.2866964232348845
107	26	-5.0697551227770985
107	27	-3.484905784081609
107	28	-3.7281452369364496
107	29	-5.9821353859815405
107	30	-1.8069550108011647
107	31	-5.374892994673215
107	32	-1.0204541185310436
107	33	-5.21302232565969
107	34	-5.349511873962424
107	35	-4.929064830163732
107	36	-4.439080851992939
107	37	-4.34465263064164
107	38	-4.262340040911468
107	39	-3.9393193802534467
107	40	-3.454535177886828
108	1	3.3469839989070813
108	2	0.11090640129101992
108	3	-0.38706479722963394
108	4	-2.572896974820047
108	5	-3.707494520131881
108	6	0.011988042482546035
108	7	8.217165648759053
108	8	11.097520641751405
108	9	7.728077569561091
108	10	3.3779425702502586
108	11	13.854201811619394
108	12	13.928112272343043
108	13	13.487045768639177
108	14	13.083753029632156
108	15	12.727009772888891
108	16	13.84814132753693
108	17	14.639945128131941
108	18	11.900047053069038
108	19	4.916861984169675
108	20	11.485557757261056
108	21	-7.693605722726604
108	22	-3.19657927542543
108	23	-2.187550026286287
108	24	-6.502710628809531
108	25	0.7133035767651155
108	26	-1.2364217894437655
108	27	-2.818239117414942
108	28	3.9385214297302173
108	29	1.3511979473517925
108	30	6.859711655865501
108	31	8.291773671993452
108	32	5.6462125481356225
108	33	9.28697767434031
108	34	4.150488126037576
108	35	9.570935169836268
108	36	2.060919148007061
108	37	10.15534736935836
108	38	10.237659959088532
108	39	6.560680619746553
108	40	11.545464822113171
109	1	9.846983998907081
109	2	-0.8890935987089801
109	3	-3.887064797229634
109	4	12.427103025179953
109	5	7.792505479868119
109	6	-6.488011957517454
109	7	6.717165648759053
109	8	7.597520641751405
109	9	2.2280775695610906
109	10	1.3779425702502586
109	11	-0.14579818838060632
109	12	1.4281122723430428
109	13	-0.5129542313608226
109	14	0.583753029632156
109	15	1.7270097728888913
109	16	2.848141327536929
109	17	3.1399451281319415
109	18	15.400047053069038
109	19	15.416861984169675
109	20	-13.514442242738944
109	21	-10.193605722726604
109	22	-9.69657927542543
109	23	-10.187550026286287
109	24	-9.502710628809531
109	25	-9.286696423234885
109	26	-7.7364217894437655
109	27	-7.318239117414942
109	28	-7.061478570269783
109	29	-6.6488020526482075
109	30	-6.140288344134499
109	31	-6.208226328006548
109	32	-5.8537874518643775
109	33	-5.21302232565969
109	34	-5.349511873962424
109	35	-4.929064830163732
109	36	-4.439080851992939
109	37	-4.34465263064164
109	38	-4.262340040911468
109	39	-3.9393193802534467
109	40	-3.454535177886828
110	1	7.846983998907081
110	2	0.11090640129101992
110	3	13.112935202770366
110	4	13.427103025179953
110	5	5.792505479868119
110	6	12.511988042482546
110	7	0.7171656487590532
110	8	7.597520641751405
110	9	12.22807756956109
110	10	7.377942570250259
110	11	10.854201811619394
110	12	3.428112272343043
110	13	-7.512954231360823
110	14	-1.416246970367844
110	15	7.727009772888891
110	16	-6.151858672463071
110	17	3.1399451281319415
110	18	10.400047053069038
110	19	-12.583138015830325
110	20	-13.514442242738944
110	21	-10.193605722726604
110	22	-9.69657927542543
110	23	-10.187550026286287
110	24	-9.502710628809531
110	25	-9.286696423234885
110	26	-7.7364217894437655
110	27	-7.318239117414942
110	28	-7.061478570269783
110	29	-6.6488020526482075
110	30	-6.140288344134499
110	31	-6.208226328006548
110	32	-5.8537874518643775
110	33	-5.21302232565969
110	34	-5.349511873962424
110	35	-4.929064830163732
110	36	-4.439080851992939
110	37	-4.34465263064164
110	38	-4.262340040911468
110	39	-3.9393193802534467
110	40	-3.454535177886828
112	1	10.132698284621366
112	2	21.53947782986245
112	3	13.970078059913224
112	4	9.712817310894238
112	5	16.649648337010973
112	6	6.797702328196831
112	7	-3.5685486369552315
112	8	1.1689492131799781
112	9	-1.7719224304389094
112	10	8.663656855964543
112	11	-0.28865533123774867
112	12	-1.0004591562283842
112	13	-1.0843828027893956
112	14	1.2980387439178713
112	15	1.012724058603176
112	16	2.848141327536929
112	17	5.1399451281319415
112	18	0.8286184816404649
112	19	0.41686198416967457
112	20	5.485557757261056
112	21	9.37782284870197
112	22	5.589135010288855
112	23	6.383878545142286
112	24	5.211575085476184
112	25	7.427589291050831
112	26	1.1207210676990922
112	27	1.5389037397279157
112	28	1.795664286873075
112	29	2.20834080449465
112	30	1.4311402272940725
112	31	-2.351083470863691
112	32	-1.9966445947215208
112	33	-2.0701651828025467
112	34	-1.4923690168195671
112	35	-1.071921973020875
112	36	-4.439080851992939
112	37	-4.34465263064164
112	38	-4.262340040911468
112	39	-3.9393193802534467
112	40	-3.454535177886828
114	1	2.8469839989070813
114	2	0.11090640129101992
114	3	2.112935202770366
114	4	9.427103025179953
114	5	8.792505479868119
114	6	9.511988042482546
114	7	9.717165648759053
114	8	5.597520641751405
114	9	4.228077569561091
114	10	1.3779425702502586
114	11	0.8542018116193937
114	12	-8.571887727656957
114	13	5.487045768639177
114	14	5.583753029632156
114	15	-13.272990227111109
114	16	11.84814132753693
114	17	11.139945128131941
114	18	-15.599952946930962
114	19	-12.583138015830325
114	20	-13.514442242738944
114	21	-10.193605722726604
114	22	-9.69657927542543
114	23	-10.187550026286287
114	24	-9.502710628809531
114	25	-9.286696423234885
114	26	-7.7364217894437655
114	27	-7.318239117414942
114	28	-7.061478570269783
114	29	-6.6488020526482075
114	30	-6.140288344134499
114	31	-6.208226328006548
114	32	-5.8537874518643775
114	33	-5.21302232565969
114	34	-5.349511873962424
114	35	-4.929064830163732
114	36	-4.439080851992939
114	37	-4.34465263064164
114	38	-4.262340040911468
114	39	-3.9393193802534467
114	40	-3.454535177886828
115	1	4.846983998907081
115	2	19.110906401291018
115	3	15.112935202770366
115	4	-5.572896974820047
115	5	8.792505479868119
115	6	7.511988042482546
115	7	12.717165648759053
115	8	9.597520641751405
115	9	13.22807756956109
115	10	-12.622057429749741
115	11	-18.145798188380606
115	12	-17.571887727656957
115	13	-18.512954231360823
115	14	-18.416246970367844
115	15	-17.27299022711111
115	16	-15.15185867246307
115	17	-15.860054871868059
115	18	-15.599952946930962
115	19	-12.583138015830325
115	20	-13.514442242738944
115	21	-10.193605722726604
115	22	-9.69657927542543
115	23	-10.187550026286287
115	24	-9.502710628809531
115	25	-9.286696423234885
115	26	-7.7364217894437655
115	27	-7.318239117414942
115	28	-7.061478570269783
115	29	-6.6488020526482075
115	30	-6.140288344134499
115	31	-6.208226328006548
115	32	-5.8537874518643775
115	33	-5.21302232565969
115	34	-5.349511873962424
115	35	-4.929064830163732
115	36	-4.439080851992939
115	37	-4.34465263064164
115	38	-4.262340040911468
115	39	-3.9393193802534467
115	40	-3.454535177886828
119	1	2.3469839989070813
119	2	6.61090640129102
119	3	-6.887064797229634
119	4	-0.07289697482004698
119	5	-10.207494520131881
119	6	-8.988011957517454
119	7	-13.782834351240947
119	8	-8.902479358248595
119	9	-13.77192243043891
119	10	-3.1220574297497414
119	11	-2.1457981883806063
119	12	-11.071887727656957
119	13	-8.512954231360823
119	14	-8.916246970367844
119	15	-9.272990227111109
119	16	-8.15185867246307
119	17	-4.8600548718680585
119	18	-12.599952946930962
119	19	-1.5831380158303254
119	20	-2.5144422427389443
119	21	3.3063942772733963
119	22	5.30342072457457
119	23	4.812449973713713
119	24	4.997289371190469
119	25	1.7133035767651155
119	26	1.7635782105562345
119	27	-4.318239117414942
119	28	5.438521429730217
119	29	1.3511979473517925
119	30	4.859711655865501
119	31	-0.20822632800654794
119	32	5.6462125481356225
119	33	-2.71302232565969
119	34	6.150488126037576
119	35	2.570935169836268
119	36	3.560919148007061
119	37	5.15534736935836
119	38	1.737659959088532
119	39	1.5606806197465533
119	40	10.045464822113171
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	749.0
5	16.0
6	31.0
7	39.0
8	29.0
9	64.0
10	115.0
11	150.0
12	172.0
13	143.0
14	157.0
15	208.0
16	211.0
17	211.0
18	219.0
19	224.0
20	201.0
21	178.0
22	172.0
23	166.0
24	164.0
25	129.0
26	148.0
27	95.0
28	59.0
29	42.0
30	23.0
31	16.0
32	3.0
33	2.0
34	1.0
35	0.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	98.93539801596903	0.6048874909266876	0.26615049600774254	0.19356399709654004
8	98.42729252359061	0.6048874909266876	0.3871279941930801	0.5806919912896201
9	98.74244256348247	0.8222490931076178	0.29020556227327693	0.14510278113663846
10	46.12037708484409	33.550882281846754	9.523809523809524	10.804931109499638
11	34.18700169122977	28.968349842957235	21.06789079487799	15.776757670935007
12	38.931851135814405	16.457225712904787	22.426292895118415	22.184630256162396
13	27.301280502536844	16.453249577192558	27.92945155834743	28.316018361923167
14	22.30007248127567	22.734960135298383	32.88233872916163	22.082628654264315
15	29.06499154385117	21.14037207054844	29.4757187726504	20.31891761294999
16	34.42860594346461	20.39139888862044	24.69195457840058	20.488040589514377
17	27.59120560521865	23.82217927035516	26.98719497463155	21.59942014979464
18	29.54820004832085	22.445035032616577	26.503986470161873	21.5027784489007
19	24.212312166747456	17.35336888027145	20.382937469704316	38.05138148327678
20	27.736168156559554	27.27712007731336	23.194008214544574	21.792703551582505
21	23.72553756946122	22.638318434404443	28.654264315051947	24.981879681082386
22	22.928243537086253	20.488040589514377	28.074414109688327	28.509301763711044
23	26.890553273737616	20.729644841749213	28.702585165498913	23.677216719014254
24	24.42618990094226	23.09736651365064	25.513409035999036	26.96303454940807
25	28.101571946795644	22.007255139056834	22.611850060459492	27.279322853688033
26	20.849514563106798	21.601941747572813	28.95631067961165	28.59223300970874
27	19.666586131915924	18.289441894177337	28.146895385358782	33.89707658854796
28	19.043015949734173	21.725471242145964	27.162880618656356	32.06863218946351
29	22.99323017408124	20.188588007736943	27.852998065764023	28.965183752417794
30	22.13888216791677	16.380353254294704	32.30099201548512	29.179772562303413
31	21.3317191283293	20.72639225181598	34.52784503631961	23.41404358353511
32	27.301280502536844	15.970041072722879	31.722638318434406	25.00604010630587
33	22.88545190913485	17.979700338327696	33.97776703721605	25.157080715321413
34	16.791495530321335	20.173955061609085	39.81638076830152	23.21816863976806
35	15.825078521381977	22.662478859627928	39.23653056293791	22.275912056052185
36	15.269388741241846	23.96714182169606	33.84875573810099	26.9147136989611
37	18.775707718364384	20.687152189692718	36.31744495523833	24.219695136704573
38	17.931367810536493	22.35379410343161	34.00193330111165	25.712904784920248
39	19.497463155351536	21.212853346218893	32.1816863976806	27.10799710074897
40	16.21164532495772	21.454457598453732	34.694370620922925	27.63952645566562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	5.0
16	7.5
17	10.0
18	10.0
19	14.0
20	18.0
21	18.5
22	19.0
23	19.0
24	37.0
25	55.0
26	44.5
27	34.0
28	34.0
29	43.0
30	52.0
31	70.0
32	88.0
33	88.0
34	87.0
35	86.0
36	108.5
37	131.0
38	131.0
39	154.0
40	177.0
41	196.0
42	215.0
43	215.0
44	239.0
45	263.0
46	255.0
47	247.0
48	247.0
49	253.5
50	260.0
51	290.0
52	320.0
53	320.0
54	311.0
55	302.0
56	295.5
57	289.0
58	289.0
59	321.0
60	353.0
61	334.0
62	315.0
63	315.0
64	321.0
65	327.0
66	276.0
67	225.0
68	225.0
69	188.0
70	151.0
71	119.5
72	88.0
73	88.0
74	70.0
75	52.0
76	41.5
77	31.0
78	31.0
79	23.5
80	16.0
81	11.5
82	7.0
83	7.0
84	4.5
85	2.0
86	1.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.1449625513409036
8	0.1449625513409036
9	0.09664170089393573
10	0.048320850446967864
11	0.0
12	0.024160425223483932
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.31408552790529115
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.09664170089393573
26	0.4590480792461948
27	0.0
28	0.024160425223483932
29	0.0724812756704518
30	0.1449625513409036
31	0.2174438270113554
32	0.0
33	0.024160425223483932
34	0.0
35	0.0
36	0.0
37	0.1449625513409036
38	0.024160425223483932
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4139.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.22565837158734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.8974358974359	91.30224691954578
2	1.9743589743589745	3.7207054844165253
3	0.5384615384615384	1.522106789079488
4	0.1794871794871795	0.6764919062575502
5	0.1794871794871795	0.8456148828219376
6	0.05128205128205128	0.2899251026818072
7	0.02564102564102564	0.16912297656438754
8	0.02564102564102564	0.19328340178787146
9	0.05128205128205128	0.4348876540227108
>10	0.07692307692307693	0.8456148828219376
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	13	0.31408552790529115	No Hit
GCGAATGGGGTTTCAATGGCTTTCTTCCCTTGACCTTTTT	12	0.2899251026818072	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	10	0.24160425223483933	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	9	0.2174438270113554	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	9	0.2174438270113554	No Hit
GCGAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	8	0.19328340178787146	No Hit
GCGAATGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC	7	0.16912297656438754	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	6	0.1449625513409036	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	6	0.1449625513409036	No Hit
GCGAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	5	0.12080212611741967	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	5	0.12080212611741967	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	5	0.12080212611741967	No Hit
GCGAATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTT	5	0.12080212611741967	No Hit
GCGAATGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	5	0.12080212611741967	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	5	0.12080212611741967	No Hit
GCGAATGGGGAAATGAAGGCCTAAGTCCAGCTGGTTTTCA	5	0.12080212611741967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.024160425223483932	0.0	0.0	0.0	0.0
16	0.024160425223483932	0.0	0.0	0.0	0.0
17	0.024160425223483932	0.0	0.0	0.0	0.0
18	0.024160425223483932	0.0	0.0	0.0	0.0
19	0.024160425223483932	0.0	0.0	0.0	0.0
20	0.024160425223483932	0.0	0.0	0.0	0.0
21	0.024160425223483932	0.0	0.0	0.0	0.0
22	0.024160425223483932	0.0	0.0	0.0	0.0
23	0.048320850446967864	0.0	0.0	0.0	0.0
24	0.048320850446967864	0.0	0.0	0.0	0.0
25	0.048320850446967864	0.0	0.0	0.0	0.0
26	0.048320850446967864	0.0	0.0	0.0	0.0
27	0.048320850446967864	0.0	0.0	0.0	0.0
28	0.048320850446967864	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAT	30	1.07806045E-5	34.333336	5
ATGGGAG	50	2.055458E-10	34.333336	5
TGGGGGG	50	2.055458E-10	34.333336	6
TGGGAGA	25	1.6000302E-4	34.333336	6
ATGGGTG	20	0.0023593202	34.333332	5
ATGGGGC	20	0.0023593202	34.333332	5
ATGGGGA	40	4.7850335E-8	34.333332	5
ATGGGAC	20	0.0023593202	34.333332	5
TGGGGGA	20	0.0023593202	34.333332	6
AATGGGG	165	0.0	34.333332	4
TGGGTAC	20	0.0023593202	34.333332	6
TGGGAAC	20	0.0023593202	34.333332	6
GCGAATG	415	0.0	33.506023	1
GAATGGG	415	0.0	33.506023	3
CGAATGG	415	0.0	33.506023	2
AATGGGA	150	0.0	33.18889	4
ATGGGGG	95	0.0	32.526314	5
AATGGGT	65	1.8189894E-12	31.692307	4
ATGGGAA	50	8.816642E-9	30.900002	5
AATGGGC	40	2.0503558E-6	30.041666	4
>>END_MODULE