FastQCFastQC Report
Fri 17 Jun 2016
SRR1525955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT693.431128791645947No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC271.3426156141223273No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT190.9448035803083044No Hit
GCGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.5469915464942815No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG100.49726504226752855No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG90.44753853804077576No Hit
GCGAATGGGAGTGCTGCGAATGGGGTTGTGTGGCTTTGGC90.44753853804077576No Hit
GCGAATGGGTACATGGTTGATCCTGCCAGTAGCATATGCT60.29835902536051717No Hit
GCGAATGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT50.24863252113376427No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.24863252113376427No Hit
GCGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24863252113376427No Hit
GCGAATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT50.24863252113376427No Hit
GCGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19890601690701143No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG40.19890601690701143No Hit
GCGAATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT40.19890601690701143No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT30.14917951268025859No Hit
GCGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT30.14917951268025859No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG30.14917951268025859No Hit
GCGAATGGGTACCTGGTTGATACTGCCAGTAGCATATGCT30.14917951268025859No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA30.14917951268025859No Hit
GCGAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC30.14917951268025859No Hit
GCGAATGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAG30.14917951268025859No Hit
GCGAATGGGGGAATTGTTTTATCCAACTCATCCCTTGACA30.14917951268025859No Hit
GCGAATGGGGGGTCAGCAGTGCTGTTAGGAATGAACTATA30.14917951268025859No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG30.14917951268025859No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC30.14917951268025859No Hit
GCGAATGGGAATCTTAAAATTCATCATTAACATTCATACA30.14917951268025859No Hit
GCGAATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAG30.14917951268025859No Hit
GCGAATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.14917951268025859No Hit
GCGAATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT30.14917951268025859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG501.1823431E-1033.655
ATGGGAT200.002291298233.655
ATGGGAG309.391582E-633.655
ATGGGAA251.4827882E-433.655
GCGAATG2000.033.651
GAATGGG2000.033.653
TGGGGGG355.8138176E-733.656
CGAATGG2000.033.652
AATGGGT251.4827882E-433.654
AATGGGG850.033.654
AATGGGA850.033.654
Produced by FastQC (version 0.11.5)