##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2011
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	17.121829935355546	22.0	4.0	30.0	4.0	33.0
2	12.765788165091994	4.0	4.0	23.0	4.0	29.0
3	12.488314271506713	4.0	4.0	21.0	4.0	30.0
4	9.349577324714073	4.0	4.0	10.0	4.0	24.0
5	9.965191447041272	4.0	4.0	15.0	4.0	23.0
6	12.791148682247638	4.0	4.0	24.0	4.0	31.0
7	12.93535554450522	4.0	4.0	25.0	4.0	30.0
8	12.508702138239682	4.0	4.0	24.0	4.0	30.0
9	11.860268523122825	4.0	4.0	22.0	4.0	29.0
10	11.37792143212332	4.0	4.0	20.0	4.0	29.0
11	12.614122327200398	4.0	4.0	24.0	4.0	33.0
12	12.183988065638985	4.0	4.0	23.0	4.0	32.0
13	11.320735952262556	4.0	4.0	20.0	4.0	31.0
14	11.627548483341622	4.0	4.0	21.0	4.0	33.0
15	11.45251118846345	4.0	4.0	21.0	4.0	33.0
16	9.401790154152163	4.0	4.0	10.0	4.0	28.0
17	8.796121332670314	4.0	4.0	9.0	4.0	26.0
18	8.896568871208354	4.0	4.0	9.0	4.0	26.0
19	8.398806563898559	4.0	4.0	6.0	4.0	24.0
20	8.283441074092492	4.0	4.0	4.0	4.0	24.0
21	8.275484833416211	4.0	4.0	4.0	4.0	25.0
22	8.310293386374937	4.0	4.0	4.0	4.0	25.0
23	8.234709099950274	4.0	4.0	4.0	4.0	25.0
24	7.9666832421680756	4.0	4.0	4.0	4.0	23.0
25	8.220288413724516	4.0	4.0	4.0	4.0	24.0
26	8.330681253107906	4.0	4.0	4.0	4.0	26.0
27	8.398806563898559	4.0	4.0	4.0	4.0	26.0
28	8.42665340626554	4.0	4.0	4.0	4.0	26.0
29	7.972650422675286	4.0	4.0	4.0	4.0	24.0
30	8.216807558428643	4.0	4.0	4.0	4.0	25.0
31	8.681253107906514	4.0	4.0	4.0	4.0	29.0
32	8.694679264047737	4.0	4.0	4.0	4.0	29.0
33	8.460467429139731	4.0	4.0	4.0	4.0	28.0
34	8.501740427647936	4.0	4.0	4.0	4.0	29.0
35	8.42864246643461	4.0	4.0	4.0	4.0	29.0
36	8.39035305818001	4.0	4.0	4.0	4.0	30.0
37	8.03033316757832	4.0	4.0	4.0	4.0	28.0
38	7.954748881153655	4.0	4.0	4.0	4.0	27.0
39	7.905519641969169	4.0	4.0	4.0	4.0	27.0
40	7.909995027349577	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.5976484420928827
1	2	3.2472075249853045
1	3	-5.659945130315503
1	4	3.146159122085047
1	5	7.346276699980404
1	6	-8.656839114246525
1	7	-7.900382128159906
1	8	-7.509151479521851
1	9	-6.806878306878309
1	10	-6.577758181461885
1	11	-6.880648638056044
1	12	-6.356986086615718
1	13	-6.157064471879286
1	14	-6.493102096805799
1	15	-5.943415637860083
1	16	-4.29564961787184
1	17	-3.727865961199295
1	18	-3.7201156182637662
1	19	-3.4648540074466005
1	20	-3.5421712718009024
1	21	-3.1463550852439752
1	22	-2.9240250832843424
1	23	-3.2489222026259066
1	24	-2.951175778953557
1	25	-2.9692925729962774
1	26	-2.9891142465216554
1	27	-3.4149323927101722
1	28	-3.1987654320987655
1	29	-2.654340583970213
1	30	-2.6755046051342353
1	31	-3.2737115422300613
1	32	-3.189271017048794
1	33	-3.1836566725455615
1	34	-3.336057221242406
1	35	-3.119116206153242
1	36	-3.025837742504409
1	37	-2.9475210660395845
1	38	-3.090035273368607
1	39	-2.5432392710170486
1	40	-2.8488732118361764
2	1	-12.902351557907117
2	2	-8.752792475014695
2	3	-9.159945130315503
2	4	-6.353840877914953
2	5	-7.153723300019596
2	6	-8.656839114246525
2	7	-7.900382128159906
2	8	-7.509151479521851
2	9	-6.806878306878309
2	10	-6.577758181461885
2	11	-6.880648638056044
2	12	-6.356986086615718
2	13	-6.157064471879286
2	14	-6.493102096805799
2	15	-5.943415637860083
2	16	-4.29564961787184
2	17	-3.727865961199295
2	18	-3.7201156182637662
2	19	-3.4648540074466005
2	20	-3.5421712718009024
2	21	-3.1463550852439752
2	22	-2.9240250832843424
2	23	-3.2489222026259066
2	24	-2.951175778953557
2	25	-2.9692925729962774
2	26	-2.9891142465216554
2	27	-3.4149323927101722
2	28	-3.1987654320987655
2	29	-2.654340583970213
2	30	-2.6755046051342353
2	31	-3.2737115422300613
2	32	-3.189271017048794
2	33	-3.1836566725455615
2	34	-3.336057221242406
2	35	-3.119116206153242
2	36	-3.025837742504409
2	37	-2.9475210660395845
2	38	-3.090035273368607
2	39	-2.5432392710170486
2	40	-2.8488732118361764
4	1	-7.502351557907117
4	2	-8.752792475014695
4	3	-9.159945130315503
4	4	-6.353840877914953
4	5	-7.153723300019596
4	6	-8.656839114246525
4	7	-7.900382128159906
4	8	-7.509151479521851
4	9	-6.806878306878309
4	10	-6.577758181461885
4	11	-6.880648638056044
4	12	-6.356986086615718
4	13	-6.157064471879286
4	14	-6.493102096805799
4	15	-5.943415637860083
4	16	-4.29564961787184
4	17	-3.727865961199295
4	18	-3.7201156182637662
4	19	-3.4648540074466005
4	20	-3.5421712718009024
4	21	-3.1463550852439752
4	22	-2.9240250832843424
4	23	-3.2489222026259066
4	24	-2.951175778953557
4	25	-2.9692925729962774
4	26	-2.9891142465216554
4	27	-3.4149323927101722
4	28	-3.1987654320987655
4	29	-2.654340583970213
4	30	-2.6755046051342353
4	31	-3.2737115422300613
4	32	-3.189271017048794
4	33	-3.1836566725455615
4	34	-3.336057221242406
4	35	-3.119116206153242
4	36	-3.025837742504409
4	37	-2.9475210660395845
4	38	-3.090035273368607
4	39	-2.5432392710170486
4	40	-2.8488732118361764
6	1	12.597648442092883
6	2	8.747207524985305
6	3	3.340054869684497
6	4	6.646159122085047
6	5	14.346276699980404
6	6	-5.656839114246525
6	7	9.599617871840094
6	8	16.49084852047815
6	9	17.19312169312169
6	10	5.422241818538115
6	11	18.619351361943956
6	12	21.14301391338428
6	13	21.842935528120712
6	14	16.0068979031942
6	15	22.056584362139915
6	16	26.704350382128162
6	17	22.272134038800704
6	18	21.779884381736235
6	19	24.0351459925534
6	20	25.457828728199097
6	21	23.353644914756025
6	22	15.075974916715658
6	23	24.251077797374094
6	24	21.548824221046445
6	25	21.030707427003723
6	26	18.010885753478345
6	27	20.58506760728983
6	28	17.801234567901233
6	29	9.845659416029786
6	30	11.824495394865764
6	31	14.22628845776994
6	32	15.810728982951206
6	33	11.316343327454438
6	34	14.663942778757594
6	35	24.380883793846756
6	36	17.474162257495593
6	37	8.552478933960415
6	38	18.909964726631394
6	39	16.45676072898295
6	40	17.651126788163822
7	1	-5.569018224573783
7	2	-0.41945914168136156
7	3	-4.826611796982169
7	4	-4.353840877914953
7	5	-5.153723300019596
7	6	-6.656839114246525
7	7	-4.900382128159906
7	8	-0.17581814618851688
7	9	0.19312169312169125
7	10	-1.244424848128551
7	11	-4.880648638056044
7	12	1.309680580050948
7	13	-1.4903978052126199
7	14	-1.8264354301391332
7	15	0.38991769547325106
7	16	2.037683715461494
7	17	4.272134038800705
7	18	4.279884381736234
7	19	-1.7981873407799336
7	20	2.7911620615324315
7	21	5.186978248089359
7	22	4.075974916715658
7	23	5.4177444640407595
7	24	-1.28450911228689
7	25	2.3640407603370566
7	26	6.677552420145011
7	27	4.251734273956494
7	28	3.4679012345679006
7	29	6.012326082696453
7	30	4.324495394865765
7	31	7.726288457769939
7	32	7.477395649617872
7	33	6.483009994121105
7	34	7.663942778757594
7	35	7.880883793846758
7	36	7.640828924162257
7	37	6.385812267293749
7	38	7.909964726631393
7	39	6.790094062316285
7	40	6.151126788163824
8	1	-2.9023515579071173
8	2	-3.9527924750146948
8	3	-3.359945130315502
8	4	-4.7538408779149535
8	5	-3.5537233000195965
8	6	-2.656839114246525
8	7	-1.1003821281599055
8	8	-1.70915147952185
8	9	-3.206878306878309
8	10	2.022241818538115
8	11	4.719351361943955
8	12	4.843013913384281
8	13	4.442935528120714
8	14	5.1068979031942
8	15	-2.943415637860083
8	16	-1.0956496178718398
8	17	-0.5278659611992946
8	18	-1.520115618263766
8	19	-3.0648540074466
8	20	-3.142171271800902
8	21	-2.746355085243975
8	22	-0.7240250832843422
8	23	-1.8489222026259062
8	24	-2.151175778953557
8	25	-0.7692925729962772
8	26	-0.3891142465216557
8	27	-1.6149323927101724
8	28	1.4012345679012341
8	29	0.9456594160297866
8	30	1.3244953948657647
8	31	3.5262884577699394
8	32	3.6107289829512066
8	33	3.016343327454438
8	34	3.4639427787575947
8	35	3.6808837938467587
8	36	3.374162257495591
8	37	3.652478933960415
8	38	2.909964726631393
8	39	4.056760728982951
8	40	2.351126788163823
9	1	15.097648442092883
9	2	-0.0027924750146954835
9	3	3.090054869684497
9	4	-4.103840877914953
9	5	-3.903723300019596
9	6	-1.1568391142465249
9	7	2.3496178718400937
9	8	-2.009151479521851
9	9	-0.5568783068783087
9	10	3.172241818538115
9	11	4.119351361943956
9	12	7.393013913384282
9	13	4.342935528120714
9	14	1.2568979031942007
9	15	4.306584362139917
9	16	6.95435038212816
9	17	-0.7278659611992948
9	18	4.529884381736234
9	19	7.5351459925533995
9	20	4.207828728199098
9	21	3.8536449147560248
9	22	0.8259749167156576
9	23	8.751077797374094
9	24	6.298824221046443
9	25	9.780707427003723
9	26	5.760885753478345
9	27	7.585067607289828
9	28	7.0512345679012345
9	29	8.345659416029786
9	30	6.824495394865765
9	31	11.72628845776994
9	32	7.060728982951206
9	33	8.316343327454438
9	34	10.913942778757594
9	35	12.380883793846758
9	36	12.22416225749559
9	37	6.8024789339604155
9	38	11.909964726631394
9	39	10.206760728982951
9	40	8.151126788163824
10	1	8.097648442092883
10	2	-2.7527924750146955
10	3	3.840054869684497
10	4	12.646159122085047
10	5	10.846276699980404
10	6	-0.6568391142465249
10	7	16.099617871840096
10	8	14.49084852047815
10	9	18.19312169312169
10	10	10.422241818538115
10	11	14.119351361943956
10	12	14.643013913384282
10	13	11.842935528120714
10	14	6.506897903194201
10	15	10.056584362139917
10	16	11.70435038212816
10	17	0.2721340388007052
10	18	18.279884381736235
10	19	18.5351459925534
10	20	22.457828728199097
10	21	13.853644914756025
10	22	13.075974916715658
10	23	22.751077797374094
10	24	18.048824221046445
10	25	21.030707427003723
10	26	24.010885753478345
10	27	18.58506760728983
10	28	18.801234567901233
10	29	7.345659416029787
10	30	22.324495394865764
10	31	25.72628845776994
10	32	22.810728982951204
10	33	22.81634332745444
10	34	27.663942778757594
10	35	22.880883793846756
10	36	14.97416225749559
10	37	23.052478933960415
10	38	26.909964726631394
10	39	12.456760728982951
10	40	12.151126788163824
11	1	2.5976484420928827
11	2	2.7472075249853045
11	3	6.340054869684497
11	4	-2.353840877914953
11	5	-0.6537233000195961
11	6	7.343160885753475
11	7	8.599617871840094
11	8	7.990848520478149
11	9	9.693121693121691
11	10	9.922241818538115
11	11	9.619351361943956
11	12	3.643013913384282
11	13	8.342935528120714
11	14	8.0068979031942
11	15	-1.9434156378600829
11	16	1.70435038212816
11	17	6.272134038800705
11	18	0.27988438173623376
11	19	1.0351459925533995
11	20	4.457828728199098
11	21	6.353644914756025
11	22	3.0759749167156576
11	23	2.7510777973740934
11	24	2.048824221046443
11	25	3.0307074270037226
11	26	-2.9891142465216554
11	27	10.085067607289828
11	28	4.3012345679012345
11	29	7.345659416029787
11	30	9.824495394865764
11	31	2.2262884577699387
11	32	3.310728982951206
11	33	6.8163433274544385
11	34	10.163942778757594
11	35	10.380883793846758
11	36	-3.025837742504409
11	37	-2.9475210660395845
11	38	-3.090035273368607
11	39	-2.5432392710170486
11	40	-2.8488732118361764
12	1	16.097648442092883
12	2	17.247207524985306
12	3	19.840054869684497
12	4	14.646159122085047
12	5	21.846276699980404
12	6	10.343160885753475
12	7	23.099617871840096
12	8	19.49084852047815
12	9	20.19312169312169
12	10	22.422241818538115
12	11	18.119351361943956
12	12	19.64301391338428
12	13	16.842935528120712
12	14	18.5068979031942
12	15	7.056584362139917
12	16	7.70435038212816
12	17	12.272134038800704
12	18	18.279884381736235
12	19	18.5351459925534
12	20	17.457828728199097
12	21	1.8536449147560248
12	22	6.075974916715658
12	23	2.7510777973740934
12	24	16.048824221046445
12	25	18.030707427003723
12	26	23.010885753478345
12	27	14.585067607289828
12	28	22.801234567901233
12	29	7.345659416029787
12	30	2.3244953948657647
12	31	20.72628845776994
12	32	17.810728982951204
12	33	20.81634332745444
12	34	22.663942778757594
12	35	20.880883793846756
12	36	26.974162257495593
12	37	27.052478933960415
12	38	22.909964726631394
12	39	10.456760728982951
12	40	27.151126788163822
13	1	-7.902351557907117
13	2	-4.352792475014695
13	3	-6.7599451303155025
13	4	-5.7538408779149535
13	5	-6.353723300019596
13	6	-5.856839114246525
13	7	-3.3003821281599066
13	8	-4.1091514795218504
13	9	-3.206878306878309
13	10	-2.777758181461885
13	11	-3.680648638056044
13	12	-1.9569860866157178
13	13	-1.3570644718792853
13	14	-2.2931020968058
13	15	-0.7434156378600836
13	16	-3.09564961787184
13	17	2.072134038800706
13	18	-2.520115618263766
13	19	-1.6648540074466007
13	20	-1.7421712718009026
13	21	1.053644914756024
13	22	0.47597491671565795
13	23	-1.4489222026259068
13	24	-0.3511757789535572
13	25	1.0307074270037226
13	26	0.6108857534783443
13	27	-0.21493239271017206
13	28	-0.5987654320987659
13	29	0.5456594160297872
13	30	0.7244953948657651
13	31	-0.27371154223006133
13	32	2.4107289829512055
13	33	-1.1836566725455615
13	34	-0.9360572212424056
13	35	0.880883793846758
13	36	-1.825837742504409
13	37	1.8524789339604162
13	38	2.309964726631393
13	39	3.256760728982952
13	40	0.9511267881638235
15	1	17.097648442092883
15	2	17.580540858318635
15	3	20.50672153635116
15	4	5.646159122085047
15	5	15.512943366647072
15	6	21.343160885753477
15	7	24.43295120517343
15	8	24.824181853811485
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115	33	-3.1836566725455615
115	34	-3.336057221242406
115	35	-3.119116206153242
115	36	-3.025837742504409
115	37	-2.9475210660395845
115	38	-3.090035273368607
115	39	-2.5432392710170486
115	40	-2.8488732118361764
116	1	-12.902351557907117
116	2	-8.752792475014695
116	3	-9.159945130315503
116	4	-6.353840877914953
116	5	-7.153723300019596
116	6	-8.656839114246525
116	7	-7.900382128159906
116	8	-7.509151479521851
116	9	-6.806878306878309
116	10	-6.577758181461885
116	11	-6.880648638056044
116	12	-6.356986086615718
116	13	-6.157064471879286
116	14	-6.493102096805799
116	15	-5.943415637860083
116	16	-4.29564961787184
116	17	-3.727865961199295
116	18	-3.7201156182637662
116	19	-3.4648540074466005
116	20	-3.5421712718009024
116	21	-3.1463550852439752
116	22	-2.9240250832843424
116	23	-3.2489222026259066
116	24	-2.951175778953557
116	25	-2.9692925729962774
116	26	-2.9891142465216554
116	27	-3.4149323927101722
116	28	-3.1987654320987655
116	29	-2.654340583970213
116	30	-2.6755046051342353
116	31	-3.2737115422300613
116	32	-3.189271017048794
116	33	-3.1836566725455615
116	34	-3.336057221242406
116	35	-3.119116206153242
116	36	-3.025837742504409
116	37	-2.9475210660395845
116	38	-3.090035273368607
116	39	-2.5432392710170486
116	40	-2.8488732118361764
117	1	-12.902351557907117
117	2	-8.752792475014695
117	3	-9.159945130315503
117	4	-6.353840877914953
117	5	-7.153723300019596
117	6	-8.656839114246525
117	7	-7.900382128159906
117	8	-7.509151479521851
117	9	-6.806878306878309
117	10	-6.577758181461885
117	11	-6.880648638056044
117	12	-6.356986086615718
117	13	-6.157064471879286
117	14	-6.493102096805799
117	15	-5.943415637860083
117	16	-4.29564961787184
117	17	-3.727865961199295
117	18	-3.7201156182637662
117	19	-3.4648540074466005
117	20	-3.5421712718009024
117	21	-3.1463550852439752
117	22	-2.9240250832843424
117	23	-3.2489222026259066
117	24	-2.951175778953557
117	25	-2.9692925729962774
117	26	-2.9891142465216554
117	27	-3.4149323927101722
117	28	-3.1987654320987655
117	29	-2.654340583970213
117	30	-2.6755046051342353
117	31	-3.2737115422300613
117	32	-3.189271017048794
117	33	-3.1836566725455615
117	34	-3.336057221242406
117	35	-3.119116206153242
117	36	-3.025837742504409
117	37	-2.9475210660395845
117	38	-3.090035273368607
117	39	-2.5432392710170486
117	40	-2.8488732118361764
120	1	-12.902351557907117
120	2	-8.752792475014695
120	3	-9.159945130315503
120	4	-6.353840877914953
120	5	-7.153723300019596
120	6	-8.656839114246525
120	7	-7.900382128159906
120	8	-7.509151479521851
120	9	-6.806878306878309
120	10	-6.577758181461885
120	11	-6.880648638056044
120	12	-6.356986086615718
120	13	-6.157064471879286
120	14	-6.493102096805799
120	15	-5.943415637860083
120	16	-4.29564961787184
120	17	-3.727865961199295
120	18	-3.7201156182637662
120	19	-3.4648540074466005
120	20	-3.5421712718009024
120	21	-3.1463550852439752
120	22	-2.9240250832843424
120	23	-3.2489222026259066
120	24	-2.951175778953557
120	25	-2.9692925729962774
120	26	-2.9891142465216554
120	27	-3.4149323927101722
120	28	-3.1987654320987655
120	29	-2.654340583970213
120	30	-2.6755046051342353
120	31	-3.2737115422300613
120	32	-3.189271017048794
120	33	-3.1836566725455615
120	34	-3.336057221242406
120	35	-3.119116206153242
120	36	-3.025837742504409
120	37	-2.9475210660395845
120	38	-3.090035273368607
120	39	-2.5432392710170486
120	40	-2.8488732118361764
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1082.0
5	42.0
6	34.0
7	47.0
8	27.0
9	53.0
10	64.0
11	58.0
12	46.0
13	47.0
14	32.0
15	22.0
16	21.0
17	16.0
18	25.0
19	23.0
20	34.0
21	62.0
22	53.0
23	65.0
24	52.0
25	36.0
26	32.0
27	19.0
28	10.0
29	7.0
30	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	98.85515181682429	0.3982080637132902	0.3982080637132902	0.34843205574912894
8	98.75621890547264	0.3482587064676617	0.6965174129353234	0.1990049751243781
9	98.00895968143355	0.6470881035340966	0.8461921353907417	0.49776007964161273
10	48.980606663351566	28.791645947289908	13.276976628543014	8.950770760815514
11	34.7090999502735	30.08453505718548	21.780208851317752	13.426156141223272
12	31.427150671307807	23.172550969666833	23.172550969666833	22.22774738935853
13	23.394723743155797	22.000995520159282	26.729716276754605	27.874564459930312
14	19.402985074626866	28.706467661691544	34.37810945273632	17.512437810945276
15	31.62605668821482	23.968175037294877	29.189457981103928	15.216310293386377
16	35.405271009448036	27.399303828940823	23.172550969666833	14.022874191944307
17	25.60914967677772	23.421183490800594	34.01292889109895	16.956737941322725
18	24.912978617603184	26.65340626553953	33.26703132769766	15.16658378915962
19	27.810945273631845	30.845771144278604	24.328358208955223	17.01492537313433
20	22.178020885131776	36.44952759820985	27.100944803580308	14.27150671307807
21	21.680755842864247	27.250124316260564	32.222774738935854	18.84634510193933
22	21.084037792143214	24.3659870711089	31.974142217802086	22.5758329189458
23	20.835405271009446	26.40477374440577	30.23371456986574	22.526106414719045
24	23.073097961213325	27.64793635007459	31.67578319244157	17.603182496270513
25	28.94867962132536	26.40757349277529	25.16193323368211	19.48181365221724
26	22.411205602801402	26.91345672836418	23.411705852926463	27.263631815907953
27	18.104738154613468	28.5785536159601	27.231920199501246	26.08478802992519
28	22.061752988047807	34.51195219123506	23.655378486055778	19.770916334661354
29	29.540918163672654	30.439121756487026	23.053892215568865	16.966067864271455
30	22.808764940239044	25.796812749003983	34.06374501992032	17.330677290836654
31	25.758329189457978	33.018398806563894	24.86325211337643	16.36001989060169
32	31.738913801694068	27.65321375186846	25.660189337319384	14.947683109118087
33	27.150671307807063	26.902038786673298	25.509696668324217	20.437593237195426
34	19.44306315266037	32.57086026852312	31.427150671307807	16.5589259075087
35	18.2496270512183	31.427150671307807	35.405271009448036	14.917951268025858
36	19.980069755854508	35.92426507224714	26.756352765321374	17.339312406576983
37	20.984584783689705	30.034808552958726	32.023868722028844	16.956737941322725
38	28.19492789656887	31.427150671307807	23.222277473893584	17.155643958229735
39	23.620089507707608	32.123321730482346	21.979114868224762	22.27747389358528
40	20.785678766782695	30.4823470909995	29.438090502237692	19.29388363998011
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	6.0
15	11.0
16	16.0
17	21.0
18	21.0
19	19.0
20	17.0
21	16.0
22	15.0
23	15.0
24	18.0
25	21.0
26	20.5
27	20.0
28	20.0
29	25.5
30	31.0
31	35.0
32	39.0
33	39.0
34	50.0
35	61.0
36	73.0
37	85.0
38	85.0
39	100.0
40	115.0
41	128.0
42	141.0
43	141.0
44	146.0
45	151.0
46	160.0
47	169.0
48	169.0
49	167.5
50	166.0
51	204.0
52	242.0
53	242.0
54	208.0
55	174.0
56	172.0
57	170.0
58	170.0
59	153.5
60	137.0
61	129.5
62	122.0
63	122.0
64	87.5
65	53.0
66	40.0
67	27.0
68	27.0
69	21.0
70	15.0
71	10.0
72	5.0
73	5.0
74	2.5
75	0.0
76	0.5
77	1.0
78	1.0
79	0.5
80	0.0
81	0.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.09945300845350571
8	0.04972650422675286
9	0.09945300845350571
10	0.0
11	0.0
12	0.0
13	0.09945300845350571
14	0.04972650422675286
15	0.0
16	0.0
17	0.0
18	0.0
19	0.04972650422675286
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.19890601690701143
26	0.5967180507210343
27	0.29835902536051717
28	0.14917951268025859
29	0.34808552958727
30	0.14917951268025859
31	0.0
32	0.19890601690701143
33	0.0
34	0.0
35	0.0
36	0.19890601690701143
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2011.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.77821979114869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.68115942028984	80.35803083043263
2	4.579710144927536	7.856787667826952
3	0.8695652173913043	2.2376926902038785
4	0.17391304347826086	0.5967180507210343
5	0.2318840579710145	0.9945300845350571
6	0.057971014492753624	0.29835902536051717
7	0.0	0.0
8	0.0	0.0
9	0.11594202898550725	0.8950770760815515
>10	0.2318840579710145	3.3316757831924413
>50	0.057971014492753624	3.431128791645947
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	69	3.431128791645947	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	27	1.3426156141223273	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	19	0.9448035803083044	No Hit
GCGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.5469915464942815	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	10	0.49726504226752855	No Hit
GCGAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	9	0.44753853804077576	No Hit
GCGAATGGGAGTGCTGCGAATGGGGTTGTGTGGCTTTGGC	9	0.44753853804077576	No Hit
GCGAATGGGTACATGGTTGATCCTGCCAGTAGCATATGCT	6	0.29835902536051717	No Hit
GCGAATGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT	5	0.24863252113376427	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	5	0.24863252113376427	No Hit
GCGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.24863252113376427	No Hit
GCGAATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	5	0.24863252113376427	No Hit
GCGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.19890601690701143	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG	4	0.19890601690701143	No Hit
GCGAATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	4	0.19890601690701143	No Hit
GCGAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT	3	0.14917951268025859	No Hit
GCGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	3	0.14917951268025859	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG	3	0.14917951268025859	No Hit
GCGAATGGGTACCTGGTTGATACTGCCAGTAGCATATGCT	3	0.14917951268025859	No Hit
GCGAATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	3	0.14917951268025859	No Hit
GCGAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	3	0.14917951268025859	No Hit
GCGAATGGGGGGTCTGGAGAGATGGCTCAGTGGATAAGAG	3	0.14917951268025859	No Hit
GCGAATGGGGGAATTGTTTTATCCAACTCATCCCTTGACA	3	0.14917951268025859	No Hit
GCGAATGGGGGGTCAGCAGTGCTGTTAGGAATGAACTATA	3	0.14917951268025859	No Hit
GCGAATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG	3	0.14917951268025859	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC	3	0.14917951268025859	No Hit
GCGAATGGGAATCTTAAAATTCATCATTAACATTCATACA	3	0.14917951268025859	No Hit
GCGAATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAG	3	0.14917951268025859	No Hit
GCGAATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	3	0.14917951268025859	No Hit
GCGAATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT	3	0.14917951268025859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGG	50	1.1823431E-10	33.65	5
ATGGGAT	20	0.0022912982	33.65	5
ATGGGAG	30	9.391582E-6	33.65	5
ATGGGAA	25	1.4827882E-4	33.65	5
GCGAATG	200	0.0	33.65	1
GAATGGG	200	0.0	33.65	3
TGGGGGG	35	5.8138176E-7	33.65	6
CGAATGG	200	0.0	33.65	2
AATGGGT	25	1.4827882E-4	33.65	4
AATGGGG	85	0.0	33.65	4
AATGGGA	85	0.0	33.65	4
>>END_MODULE