FastQCFastQC Report
Fri 17 Jun 2016
SRR1525954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT530.3915773919468046No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC420.31030661248614705No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT410.30291835980790544No Hit
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG330.2438123383819727No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC280.20687107499076468No Hit
GTCCCGGGGGACAGGATCAACCATAGGAAAGAAGATTCCTGAGCCGGGCATGGTGA270.19948282231252307No Hit
GTCCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC240.1773180642777983No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC220.1625415589213151No Hit
GTCCCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT210.15515330624307352No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT190.14037680088659032No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC190.14037680088659032No Hit
GTCCCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC170.12560029553010713No Hit
GTCCCGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA160.11821204285186553No Hit
GTCCCGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC150.11082379017362395No Hit
GTCCCGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC150.11082379017362395No Hit
GTCCCGGGGGTTCTTTGGGATATAGCATTCTTCTCATGGTCACATGTACTGTGTAT150.11082379017362395No Hit
GTCCCGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC140.10343553749538234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC203.89142E-450.3707943
CAATCCG203.89142E-450.3707942
TCCGCCC203.89142E-450.3707945
ATCCGCC203.89142E-450.3707944
CACTGTC150.00801330950.37078542
CACATCC150.00813327450.1828413
CATCCTC150.00813327450.1828415
ACATCCT150.00813327450.1828414
GCAGTGG203.9644205E-450.1828412
AGAGCCC150.00813327450.1828436
GGGCCCC150.00813327450.182849
CCGCCCA251.9358307E-550.18283546
AGCAGTG251.9358307E-550.18283511
AAGAGCC204.0385048E-449.99628435
GCCCAAT150.0082545749.9962848
CCCAATC150.0082545749.9962849
TCAACGC204.113685E-449.81111521
GGGAGCT204.113685E-449.8111158
GGGATCA204.113685E-449.8111157
CAACGCA204.113685E-449.81111522