##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525954_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13535 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.39534540081271 39.0 38.0 40.0 33.0 40.0 2 37.747765053564834 39.0 38.0 40.0 35.0 40.0 3 37.58973032877724 39.0 38.0 40.0 35.0 40.0 4 37.34613963797562 39.0 38.0 40.0 33.0 40.0 5 37.346656815663096 39.0 38.0 40.0 34.0 40.0 6 36.51895086811969 38.0 36.0 39.0 31.0 40.0 7 36.73394902105652 39.0 36.0 39.0 31.0 40.0 8 36.653934244551166 39.0 36.0 39.0 31.0 40.0 9 36.79468045807167 39.0 37.0 39.0 32.0 40.0 10 36.33542667159217 39.0 36.0 39.0 31.0 40.0 11 34.700480236424085 38.0 34.0 39.0 26.0 40.0 12 33.8562984854082 38.0 32.0 39.0 22.0 40.0 13 33.51843369043221 38.0 32.0 39.0 20.0 40.0 14 34.12759512375323 38.0 33.0 39.0 23.0 40.0 15 33.11643886220909 38.0 31.0 39.0 18.0 40.0 16 32.36867380864425 36.0 30.0 39.0 18.0 40.0 17 31.51281861839675 36.0 28.0 38.0 15.0 40.0 18 30.75382342076099 35.0 26.0 38.0 13.0 40.0 19 31.79231621721463 36.0 29.0 38.0 16.0 40.0 20 32.51991134096786 36.0 30.0 39.0 19.0 40.0 21 32.47661618027337 37.0 30.0 39.0 17.0 40.0 22 31.760103435537495 36.0 28.0 39.0 15.0 40.0 23 32.3957886959734 37.0 30.0 39.0 17.0 40.0 24 31.900110823790175 36.0 29.0 39.0 15.0 40.0 25 32.22452899889176 36.0 29.0 39.0 17.0 40.0 26 32.60081270779461 37.0 31.0 39.0 17.0 40.0 27 32.87048393055043 37.0 31.0 39.0 19.0 40.0 28 32.89405245659402 37.0 31.0 39.0 19.0 40.0 29 32.31097155522719 36.0 30.0 39.0 17.0 40.0 30 32.142593276690064 36.0 30.0 39.0 15.0 40.0 31 32.896195049870705 38.0 33.0 39.0 17.0 40.0 32 32.74879940893979 37.0 32.0 39.0 17.0 40.0 33 32.566309567787215 37.0 31.0 39.0 17.0 40.0 34 32.29516069449575 36.0 31.0 39.0 15.0 40.0 35 32.144218692279274 36.0 31.0 38.0 13.0 40.0 36 31.83856667898042 36.0 31.0 38.0 10.0 40.0 37 31.529442186922793 36.0 30.0 38.0 4.0 40.0 38 30.88784632434429 36.0 29.0 38.0 4.0 40.0 39 29.774436645733285 35.0 27.0 38.0 4.0 40.0 40 27.970890284447727 33.0 20.0 38.0 4.0 39.0 41 26.288585149612118 32.0 16.0 38.0 4.0 39.0 42 26.981824898411524 32.0 19.0 38.0 4.0 39.0 43 27.032803841891393 33.0 19.0 38.0 4.0 39.0 44 26.03974879940894 31.0 15.0 37.0 4.0 39.0 45 26.916438862209088 33.0 19.0 38.0 4.0 39.0 46 24.82415958625785 30.0 11.0 36.0 4.0 39.0 47 23.841078684891023 28.0 8.0 36.0 4.0 39.0 48 24.450609530845956 29.0 10.0 36.0 4.0 39.0 49 23.409530845954933 28.0 4.0 35.0 4.0 39.0 50 23.98677502770595 29.0 4.0 36.0 4.0 39.0 51 24.165866272626523 30.0 4.0 36.0 4.0 39.0 52 23.98965644625046 30.0 4.0 36.0 4.0 39.0 53 23.908681196896932 30.0 4.0 36.0 4.0 39.0 54 23.480310306612488 29.0 4.0 36.0 4.0 39.0 55 23.02164758034725 29.0 4.0 35.0 4.0 38.0 56 22.402290358330255 28.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.6574306684880895 1 2 0.7389602866040192 1 3 0.07696802709338613 1 4 1.7635346515450792 1 5 1.9603238625886021 1 6 1.874867861943173 1 7 2.5923165498810334 1 8 2.7691700573494558 1 9 2.6866373297312833 1 10 2.1487097287372023 1 11 -1.0679011378681125 1 12 -6.349614979815421 1 13 -17.653816031694994 1 14 -7.023227324896624 1 15 -0.3012937646245675 1 16 -4.3242192415935 1 17 -8.774902150313615 1 18 -7.224985626755334 1 19 -1.1047492575423234 1 20 -1.320661785116524 1 21 4.5203372871065355 1 22 6.510482728057816 1 23 5.292762414493247 1 24 4.425075510302374 1 25 4.435359725910406 1 26 6.250297484134013 1 27 5.45915907093703 1 28 6.208845999645611 1 29 6.404890402635353 1 30 6.381092236444825 1 31 1.8820334509370689 1 32 3.95174510637003 1 33 5.110728956541482 1 34 5.266056293441437 1 35 2.249655508944489 1 36 0.7210115207928389 1 37 1.8709036278565883 1 38 3.2859199888244817 1 39 2.1014545843263086 1 40 -11.172544565172446 1 41 -1.0852642279601206 1 42 -2.2650126080496094 1 43 -8.91792626654111 1 44 -14.981558263755062 1 45 -3.3090225238575 1 46 -7.972190592706479 1 47 -11.822634274184532 1 48 -5.09444358648399 1 49 -12.964359982267888 1 50 -3.6932852905841713 1 51 2.6333735358171673 1 52 2.415436684041861 1 53 2.0615088579353618 1 54 1.7760273989445174 1 55 2.395416077783242 1 56 5.691438565026072 2 1 1.2407640018214252 2 2 1.0445158421595693 2 3 1.2853013604267218 2 4 1.652423540433972 2 5 1.3492127514774879 2 6 2.8470900841653943 2 7 2.5784276609921477 2 8 2.449725612905013 2 9 2.3116373297312833 2 10 2.2737097287372023 2 11 3.7654321954652232 2 12 -2.9607260909265314 2 13 0.24896174608278443 2 14 3.7823282306589334 2 15 3.226484013153211 2 16 4.495225202850946 2 17 5.058431183019717 2 18 3.038903262133555 2 19 5.103584075791009 2 20 4.665449325994583 2 21 5.964781731550978 2 22 5.774371616946702 2 23 3.7233179700488037 2 24 4.341742176969046 2 25 3.657581948132627 2 26 2.097519706356234 2 27 3.6397146264925873 2 28 2.6532904440900538 2 29 4.363223735968688 2 30 3.6727589031114967 2 31 4.257033450937069 2 32 4.048967328592251 2 33 3.3607289565414824 2 34 2.016056293441437 2 35 4.2079888422778176 2 36 3.1099004096817247 2 37 2.523681405634367 2 38 3.452586655491153 2 39 4.545899028770755 2 40 2.8135665459386665 2 41 4.90084688315099 2 42 4.24887628083928 2 43 2.7070737334588877 2 44 -5.787113819310619 2 45 3.6631996983647213 2 46 2.4861427406268533 2 47 0.21903239248213424 2 48 2.5583341912937883 2 49 -4.728248871156776 2 50 2.8483813760824965 2 51 5.911151313594946 2 52 5.457103350708529 2 53 6.1448421912686975 2 54 6.84547184338896 2 55 3.853749411116574 2 56 6.705327453914961 3 1 2.640764001821424 3 2 1.9278491754929021 3 3 1.9103013604267218 3 4 2.4190902071006377 3 5 2.4492127514774893 3 6 3.4637567508320615 3 7 3.4034276609921505 3 8 3.1747256129050143 3 9 3.4366373297312833 3 10 3.615376395403864 3 11 5.4320988621318875 3 12 5.355940575740135 3 13 5.590628412749446 3 14 5.782328230658933 3 15 2.726484013153211 3 16 7.586891869517608 3 17 8.241764516353054 3 18 9.530569928800226 3 19 8.061917409124344 3 20 7.573782659327922 3 21 7.298115064884314 3 22 8.382704950280036 3 23 7.998317970048802 3 24 7.808408843635707 3 25 8.124248614799289 3 26 6.405853039689571 3 27 6.064714626492584 3 28 6.636623777423388 3 29 7.5382237359686854 3 30 7.1144255697781595 3 31 5.915366784270407 3 32 5.440633995258917 3 33 5.877395623208148 3 34 6.299389626774776 3 35 7.316322175611152 3 36 7.19323374301506 3 37 6.298681405634365 3 38 8.269253322157816 3 39 10.21256569543742 3 40 9.871899879272 3 41 11.692513549817658 3 42 12.49054294750595 3 43 11.298740400125553 3 44 12.879552847356049 3 45 11.363199698364724 3 46 13.377809407293523 3 47 14.260699059148802 3 48 13.583334191293787 3 49 14.68008446217656 3 50 14.356714709415826 3 51 14.461151313594943 3 52 13.365436684041864 3 53 15.028175524602027 3 54 15.120471843388959 3 55 15.328749411116576 3 56 14.080327453914961 4 1 2.390764001821424 4 2 1.2278491754929064 4 3 1.460301360426719 4 4 2.269090207100639 4 5 2.149212751477485 4 6 2.563756750832056 4 7 3.2034276609921477 4 8 3.1747256129050143 4 9 2.7866373297312848 4 10 2.5153763954038695 4 11 5.482098862131885 4 12 5.7059405757401365 4 13 6.490628412749452 4 14 6.132328230658935 4 15 5.976484013153211 4 16 8.486891869517613 4 17 9.141764516353053 4 18 9.330569928800223 4 19 8.811917409124344 4 20 6.773782659327917 4 21 7.6481150648843155 4 22 7.882704950280036 4 23 7.048317970048807 4 24 7.4084088436357085 4 25 7.074248614799291 4 26 7.005853039689573 4 27 5.21471462649259 4 28 5.286623777423387 4 29 5.488223735968688 4 30 5.814425569778162 4 31 5.015366784270402 4 32 5.190633995258917 4 33 4.827395623208144 4 34 6.449389626774774 4 35 5.766322175611155 4 36 6.593233743015059 4 37 6.148681405634367 4 38 6.9192533221578145 4 39 6.2625656954374165 4 40 -1.8281001207280028 4 41 8.29251354981766 4 42 9.14054294750595 4 43 8.448740400125558 4 44 9.97955284735605 4 45 8.263199698364723 4 46 11.67780940729352 4 47 12.6606990591488 4 48 12.683334191293788 4 49 12.93008446217656 4 50 12.556714709415829 4 51 12.361151313594949 4 52 12.465436684041858 4 53 12.378175524602028 4 54 13.120471843388959 4 55 13.978749411116574 4 56 9.830327453914961 5 1 2.036218547275972 5 2 1.446030993674725 5 3 1.9557559058812686 5 4 2.364544752555183 5 5 1.6219400242047612 5 6 2.4455749326502385 5 7 2.976154933719421 5 8 2.779271067450466 5 9 2.3230009660949165 5 10 1.3608309408584134 5 11 4.477553407586434 5 12 3.97866784846741 5 13 4.472446594567629 5 14 4.57778277611348 5 15 6.294665831335031 5 16 7.332346414972157 5 17 7.573582698171233 5 18 7.330569928800223 5 19 7.789190136397071 5 20 5.578328113873372 5 21 5.798115064884314 5 22 6.050886768461858 5 23 5.57559069732153 5 24 5.4856815709084366 5 25 6.687884978435658 5 26 3.3513075851441165 5 27 2.6056237174016808 5 28 4.895714686514296 5 29 3.6927691905141415 5 30 3.7598801152327077 5 31 2.7153667842704046 5 32 2.249724904349822 5 33 2.36830471411724 5 34 2.96302599041114 5 35 2.9617767210657 5 36 1.670506470287787 5 37 2.1941359510889136 5 38 5.2556169585214505 5 39 2.439838422710146 5 40 4.817354424726545 5 41 7.601604458908568 5 42 8.063270220233221 5 43 6.294194945580102 5 44 7.402280120083322 5 45 6.731381516546538 5 46 10.709627589111701 5 47 10.510699059148802 5 48 9.910606918566515 5 49 10.070993553085646 5 50 8.897623800324919 5 51 8.820242222685856 5 52 6.279073047678224 5 53 8.046357342783846 5 54 8.856835479752597 5 55 5.001476683843848 5 56 5.989418363005871 6 1 2.8385900887779485 6 2 2.1061100450581236 6 3 1.3885622299919405 6 4 2.797351076665855 6 5 2.740517099303574 6 6 2.9159306638755353 6 7 2.9860363566443198 6 8 2.5855951781224036 6 9 2.88228950364433 6 10 3.6849416127951713 6 11 5.888620601262325 6 12 7.0972449235662225 6 13 6.3558458040538 6 14 6.662763013267629 6 15 7.541701404457562 6 16 8.134717956474134 6 17 9.326547125048702 6 18 10.221874276626309 6 19 8.866265235211298 6 20 8.102043528893137 6 21 8.232897673579963 6 22 8.863139732888733 6 23 7.848317970048804 6 24 8.432321887113968 6 25 8.432944266973205 6 26 6.827592170124351 6 27 5.449497235188236 6 28 7.160536820901648 6 29 6.346919388142602 6 30 7.236164700212942 6 31 7.454497219053014 6 32 6.405851386563267 6 33 7.668699971034233 6 34 6.164607018079124 6 35 5.720670001698107 6 36 7.291059829971584 6 37 7.279116188243062 6 38 8.42360114824477 6 39 8.47343526065481 6 40 7.663204227098085 6 41 12.0186005063394 6 42 12.138369034462471 6 43 12.683523008821204 6 44 11.999118064747353 6 45 10.89146056792994 6 46 14.26693984207613 6 47 14.293307754800974 6 48 12.835508104337265 6 49 12.849649679567861 6 50 14.850192970285391 6 51 11.498107835334078 6 52 13.480654075346212 6 53 12.249914655036811 6 54 12.176993582519398 6 55 13.163532019812223 6 56 11.732501366958438 7 1 1.0407640018214224 7 2 0.7778491754929036 7 3 0.710301360426719 7 4 1.6190902071006334 7 5 1.7492127514774864 7 6 1.7637567508320586 7 7 1.9034276609921505 7 8 2.324725612905013 7 9 2.336637329731282 7 10 3.165376395403868 7 11 5.13209886213189 7 12 5.755940575740134 7 13 5.040628412749449 7 14 3.4823282306589363 7 15 4.5764840131532125 7 16 6.286891869517611 7 17 7.44176451635305 7 18 7.580569928800223 7 19 7.011917409124347 7 20 6.123782659327919 7 21 6.6481150648843155 7 22 7.782704950280035 7 23 5.998317970048802 7 24 4.358408843635711 7 25 6.674248614799293 7 26 3.7558530396895726 7 27 4.96471462649259 7 28 5.636623777423388 7 29 4.488223735968688 7 30 5.564425569778162 7 31 5.915366784270407 7 32 4.390633995258913 7 33 4.527395623208147 7 34 3.64938962677477 7 35 6.316322175611152 7 36 2.6932337430150604 7 37 3.948681405634364 7 38 5.119253322157817 7 39 6.112565695437418 7 40 5.221899879271998 7 41 9.692513549817658 7 42 9.64054294750595 7 43 10.148740400125554 7 44 7.1795528473560495 7 45 5.06319969836472 7 46 8.077809407293522 7 47 6.710699059148801 7 48 6.833334191293787 7 49 8.280084462176557 7 50 5.756714709415828 7 51 5.361151313594945 7 52 5.915436684041861 7 53 6.378175524602028 7 54 7.020471843388961 7 55 6.878749411116573 7 56 6.98032745391496 8 1 2.871716382773805 8 2 1.7707063183500509 8 3 1.815063265188627 8 4 2.1762330642434904 8 5 2.206355608620342 8 6 3.073280560355869 8 7 2.679618137182622 8 8 3.277106565285962 8 9 3.0437801868741374 8 10 3.529662109689582 8 11 6.170194100227128 8 12 6.324988194787757 8 13 6.790628412749449 8 14 6.770423468754174 8 15 7.357436394105591 8 16 8.263082345708085 8 17 9.415574040162578 8 18 10.544855643085938 8 19 7.990488837695775 8 20 8.338068373613634 8 21 7.798115064884314 8 22 8.685085902660987 8 23 8.18165130338214 8 24 8.4488850341119 8 25 7.824248614799291 8 26 7.5915673254038545 8 27 6.871857483635441 8 28 6.891385682185295 8 29 7.642985640730593 8 30 7.047758903111497 8 31 6.143938212841835 8 32 6.69777685240177 8 33 6.277395623208147 8 34 7.408913436298583 8 35 6.273465032754007 8 36 6.276567076348396 8 37 7.339157596110557 8 38 8.309729512634007 8 39 9.83161331448504 8 40 3.319518926891046 8 41 12.049656406960512 8 42 11.266733423696422 8 43 12.201121352506505 8 44 12.939076656879859 8 45 11.651294936459962 8 46 13.146857026341142 8 47 14.939270487720233 8 48 15.088096096055693 8 49 14.075322557414651 8 50 14.330524233225354 8 51 13.744484646928282 8 52 14.67734144594662 8 53 15.061508857935365 8 54 14.315709938627055 8 55 15.681130363497523 8 56 14.127946501534012 9 1 2.490764001821425 9 2 1.627849175492905 9 3 1.5769680270933861 9 4 2.152423540433972 9 5 2.1269905292552664 9 6 2.7082011952765015 9 7 2.4812054387699263 9 8 2.6580589462383486 9 9 2.2421928852868405 9 10 3.426487506514981 9 11 3.9876544176874447 9 12 5.2059405757401365 9 13 5.957295079416113 9 14 5.310106008436712 9 15 7.365372902042097 9 16 7.286891869517611 9 17 7.780653405241939 9 18 9.552792151022445 9 19 6.839695186902123 9 20 7.679338214883476 9 21 6.6870039537732 9 22 6.677149394724481 9 23 6.792762414493247 9 24 7.813964399191267 9 25 6.60202639257707 9 26 7.30585303968957 9 27 7.903603515381473 9 28 5.875512666312275 9 29 6.904890402635353 9 30 7.214425569778161 9 31 6.882033450937069 9 32 6.618411773036694 9 33 6.999617845430368 9 34 6.65494518233033 9 35 4.0274332867222675 9 36 7.4987892985706175 9 37 6.9820147389677025 9 38 7.285919988824482 9 39 7.7681212509929765 9 40 4.549677657049777 9 41 10.748069105373215 9 42 12.068320725283726 9 43 11.35985151123667 9 44 12.407330625133827 9 45 11.135421920586943 9 46 13.361142740626857 9 47 14.121810170259916 9 48 14.738889746849345 9 49 15.14675112884322 9 50 13.695603598304714 9 51 13.355595758039389 9 52 15.026547795152975 9 53 11.394842191268694 9 54 13.164916287833403 9 55 13.11763830000546 9 56 12.469216342803847 10 1 2.2802376860319526 10 2 1.8383754912823775 10 3 1.9103013604267218 10 4 2.5822481018374788 10 5 2.5597390672669604 10 6 2.632177803463641 10 7 3.098164503097408 10 8 3.272094033957643 10 9 2.449795224468126 10 10 3.4469553427722843 10 11 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0.4907640018214252 115 2 -1.0388174911737593 115 3 -0.4230319729066139 115 4 0.4857568737673006 115 5 0.26587941814415217 115 6 0.4304234174987229 115 7 1.0367609943254834 115 8 0.4913922795716772 115 9 0.769970663064619 115 10 0.5653763954038666 115 11 -1.3179011378681125 115 12 -4.0440594242598635 115 13 -2.376038253917219 115 14 -2.8843384360077344 115 15 -6.523515986846789 115 16 4.536891869517611 115 17 3.9750978496863887 115 18 5.997236595466887 115 19 4.89525074245768 115 20 4.873782659327919 115 21 4.714781731550978 115 22 5.0660382836133735 115 23 5.514984636715468 115 24 6.25840884363571 115 25 4.740915281465956 115 26 4.05585303968957 115 27 4.014714626492587 115 28 5.569957110756725 115 29 5.321557069302024 115 30 5.214425569778161 115 31 4.632033450937069 115 32 0.8406339952589157 115 33 5.444062289874811 115 34 3.9327229601081086 115 35 4.8329888422778176 115 36 4.276567076348396 115 37 4.148681405634367 115 38 4.119253322157817 115 39 6.045899028770755 115 40 7.271899879271999 115 41 7.109180216484326 115 42 7.8738762808392835 115 43 4.582073733458888 115 44 -2.2037804859772834 115 45 8.996533031698057 115 46 0.6111427406268533 115 47 -7.072634274184532 115 48 -0.9833324753728796 115 49 -2.853248871156776 115 50 2.3067147094158287 115 51 2.744484646928278 115 52 4.332103350708529 115 53 4.97817552460203 115 54 2.637138510055628 115 55 4.728749411116574 115 56 7.996994120581629 116 1 2.178264001821425 116 2 0.565349175492905 116 3 1.5353013604267218 116 4 1.8190902071006363 116 5 2.036712751477488 116 6 2.3262567508320586 116 7 1.7659276609921477 116 8 2.199725612905013 116 9 1.9366373297312833 116 10 2.6903763954038666 116 11 3.1195988621318875 116 12 3.9559405757401365 116 13 3.9156284127494487 116 14 5.594828230658933 116 15 -2.336015986846789 116 16 4.724391869517611 116 17 5.454264516353053 116 18 4.768069928800223 116 19 3.8119174091243444 116 20 3.1862826593279188 116 21 4.360615064884314 116 22 4.045204950280038 116 23 2.4733179700488037 116 24 4.00840884363571 116 25 2.7617486147992913 116 26 4.86835303968957 116 27 3.7647146264925873 116 28 4.4241237774233895 116 29 4.050723735968688 116 30 5.526925569778161 116 31 4.090366784270405 116 32 4.278133995258916 116 33 4.589895623208147 116 34 4.224389626774773 116 35 3.5413221756111533 116 36 4.50573374301506 116 37 3.711181405634367 116 38 4.431753322157817 116 39 6.025065695437419 116 40 8.146899879271999 116 41 5.130013549817658 116 42 2.540542947505948 116 43 0.9362404001255555 116 44 3.1920528473560488 116 45 4.663199698364721 116 46 2.027809407293521 116 47 -0.9268009408511979 116 48 1.4958341912937883 116 49 2.0425844621765563 116 50 3.1817147094158287 116 51 6.911151313594946 116 52 7.665436684041861 116 53 7.66567552460203 116 54 7.84547184338896 116 55 7.228749411116574 116 56 6.330327453914961 117 1 -0.30923599817857195 117 2 -0.47215082450709644 117 3 -0.8896986395732753 117 4 0.019090207100639134 117 5 0.34921275147748787 117 6 -0.23624324916794137 117 7 -0.49657233900785513 117 8 0.4247256129050143 117 9 -1.7133626702687152 117 10 0.8153763954038666 117 11 -1.5679011378681125 117 12 -12.394059424259865 117 13 -4.10937158725055 117 14 -4.067671769341068 117 15 -13.82351598684679 117 16 -7.413108130482389 117 17 -0.20823548364694844 117 18 -5.769430071199778 117 19 -0.2380825908756563 117 20 -2.5762173406720805 117 21 3.1981150648843126 117 22 4.132704950280036 117 23 3.148317970048801 117 24 3.1584088436357085 117 25 1.8242486147992913 117 26 3.2058530396895684 117 27 1.0147146264925873 117 28 0.7866237774233902 117 29 0.7382237359686883 117 30 2.3144255697781624 117 31 1.6153667842704031 117 32 -0.5593660047410829 117 33 -1.3226043767918547 117 34 2.1993896267747743 117 35 -0.8836778243888475 117 36 1.9432337430150604 117 37 3.448681405634364 117 38 3.019253322157816 117 39 5.212565695437419 117 40 6.671899879271997 117 41 3.7925135498176594 117 42 1.1905429475059464 117 43 -7.051259599874445 117 44 0.4295528473560495 117 45 1.213199698364722 117 46 -1.472190592706479 117 47 -4.289300940851199 117 48 -3.1166658087062125 117 49 -1.719915537823443 117 50 1.40671470941583 117 51 6.2111513135949465 117 52 5.715436684041862 117 53 2.628175524602028 117 54 3.3204718433889617 117 55 2.9287494111165735 117 56 4.98032745391496 118 1 1.4907640018214252 118 2 0.17330372094745172 118 3 0.6375740876994485 118 4 1.3190902071006363 118 5 1.3492127514774879 118 6 1.2637567508320586 118 7 1.0216094791739678 118 8 1.7792710674504661 118 9 1.3684555115494632 118 10 1.8153763954038666 118 11 -0.43153750150447934 118 12 -11.203150333350774 118 13 -9.936644314523278 118 14 -7.83130813297743 118 15 -7.114425077755879 118 16 -3.758562675936936 118 17 1.1644917890803264 118 18 -7.62397552574523 118 19 0.10737195457889115 118 20 1.2601462956915555 118 21 6.8890241557934075 118 22 5.823614041189131 118 23 6.211954333685171 118 24 6.894772479999343 118 25 6.006066796617471 118 26 6.48767122150775 118 27 5.923805535583494 118 28 6.077532868332483 118 29 6.1927691905141415 118 30 6.6235164788690675 118 31 4.806275875179498 118 32 5.022452177077096 118 33 4.91375925957178 118 34 3.1448441722293197 118 35 4.32541308470206 118 36 7.397779197560514 118 37 6.60322686017982 118 38 5.391980594885091 118 39 6.394383877255599 118 40 7.6355362429083655 118 41 6.556149913454021 118 42 6.154179311142311 118 43 0.1578313092164656 118 44 1.6750073928105955 118 45 7.322290607455631 118 46 2.7096275891117045 118 47 -0.6711191226693813 118 48 2.001516009475605 118 49 2.525539007631103 118 50 3.170351073052192 118 51 7.592969495413129 118 52 6.915436684041861 118 53 4.182720979147483 118 54 5.584108207025324 118 55 7.0923857747529375 118 56 9.716691090278598 119 1 1.5816730927305187 119 2 0.7187582664019985 119 3 1.0012104513358153 119 4 1.4099992980097298 119 5 1.4401218423865814 119 6 2.2637567508320586 119 7 2.294336751901241 119 8 1.7792710674504661 119 9 2.277546420640377 119 10 2.8153763954038666 119 11 -0.5224465924135657 119 12 -9.11224124244168 119 13 -4.754826132705098 119 14 -9.285853587522883 119 15 -11.568970532301336 119 16 -11.394926312300573 119 17 1.6190372436257796 119 18 -2.078520980290687 119 19 1.0164628636697977 119 20 -1.7398537043084445 119 21 3.161751428520681 119 22 4.732704950280038 119 23 4.211954333685171 119 24 2.3493179345448034 119 25 4.278794069344745 119 26 5.396762130598663 119 27 5.105623717401681 119 28 3.9866237774233895 119 29 5.829132826877782 119 30 6.8053346606872545 119 31 5.260821329724951 119 32 5.022452177077096 119 33 4.277395623208147 119 34 6.053935081320226 119 35 2.598140357429333 119 36 6.5795973793786935 119 37 7.42140867836164 119 38 7.391980594885091 119 39 8.667111149982873 119 40 10.544627151999272 119 41 9.465240822544931 119 42 7.4269065838695845 119 43 4.3396494910346455 119 44 2.2204619382651387 119 45 9.595017880182901 119 46 1.2550821345662477 119 47 0.9652445136942553 119 48 -3.8166658087062117 119 49 0.34372082581291963 119 50 4.170351073052192 119 51 8.502060404504036 119 52 9.460891229496408 119 53 10.364539160965666 119 54 9.31138093429805 119 55 10.274203956571121 119 56 8.716691090278598 120 1 1.672582183639605 120 2 0.5369400845838115 120 3 1.0921195422449017 120 4 1.5009083889188162 120 5 1.5310309332956678 120 6 2.172847659922965 120 7 1.7488822064466945 120 8 2.324725612905013 120 9 1.91391005700401 120 10 2.9971945772220465 120 11 -1.4315375015044758 120 12 -12.748604878805317 120 13 -13.663917041796005 120 14 -5.5585808602501565 120 15 1.6128476495168442 120 16 -10.485835403209663 120 17 -1.8355082109196736 120 18 -7.260339162108867 120 19 -0.710809863602929 120 20 -1.1034900679448079 120 21 4.798115064884314 120 22 6.641795859370944 120 23 5.121045242776077 120 24 6.25840884363571 120 25 5.551521342072018 120 26 5.942216676053203 120 27 6.832896444674407 120 28 6.168441959241569 120 29 7.556405554150508 120 30 7.168971024323614 120 31 5.624457693361311 120 32 5.113361267986189 120 33 4.8228501686626934 120 34 5.417571444956593 120 35 6.416322175611153 120 36 7.034142833924154 120 37 7.148681405634367 120 38 7.664707867612364 120 39 9.121656604528326 120 40 9.544627151999272 120 41 0.9197862770903846 120 42 -2.027638870675869 120 43 -2.933077781692628 120 44 -13.68862897082577 120 45 2.231381516546538 120 46 -7.835826956342842 120 47 -4.671119122669381 120 48 -0.18030217234257506 120 49 -14.56537008327799 120 50 0.07944198214310205 120 51 7.411151313594946 120 52 6.642709411314588 120 53 6.63726643369294 120 54 7.765926388843507 120 55 8.183294865662027 120 56 10.080327453914961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 75.0 5 16.0 6 71.0 7 49.0 8 58.0 9 155.0 10 77.0 11 83.0 12 92.0 13 44.0 14 51.0 15 62.0 16 59.0 17 71.0 18 118.0 19 113.0 20 163.0 21 192.0 22 219.0 23 316.0 24 384.0 25 401.0 26 456.0 27 482.0 28 500.0 29 515.0 30 600.0 31 674.0 32 695.0 33 766.0 34 895.0 35 922.0 36 970.0 37 1152.0 38 1127.0 39 912.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.04691540450683 0.6353897303287772 0.10343553749538234 0.21425932766900627 8 99.19468045807167 0.5984484669375693 0.14037680088659032 0.06649427410417437 9 96.46841521980052 1.7140746213520501 1.1599556704839304 0.657554488363502 10 59.20206871074991 23.65718507572959 7.617288511267086 9.523457702253417 11 40.096047284817146 25.637236793498335 17.02253417066864 17.244181751015887 12 36.6605097894348 16.357591429626893 20.975249353527893 26.006649427410416 13 24.63243442925748 14.702622829700775 24.24824528998892 36.416697451052826 14 21.43332101957887 24.920576283708904 27.23309937199852 26.413003324713706 15 29.661520190023754 20.056413301662708 24.1166864608076 26.165380047505938 16 32.160283897678546 22.38651486026911 25.048055596628714 20.405145645423627 17 31.968666863730416 21.741058232338162 25.369494531480935 20.920780372450487 18 30.195788695973402 20.768378278537124 23.41337273734762 25.622460288141852 19 28.309557247394483 25.16076576243625 22.37415921354128 24.155517776627985 20 29.744120692205296 25.380860819405417 24.24197603904748 20.63304244934181 21 29.043221278167714 21.049131880310306 25.881049131880314 24.02659770964167 22 24.477281123014407 22.002216475803476 27.48429996305874 26.036202438123386 23 24.728481714074622 24.063538973032877 27.683782785371257 23.52419652752124 24 24.935352789065384 24.15958625785002 24.44772811230144 26.457332840783153 25 25.59479828579873 25.306635141126055 22.713166839071967 26.38539973400325 26 30.036223848599096 24.52872033710357 21.098543653433875 24.336512160863457 27 23.578978490649718 23.86724813363885 23.39419025796437 29.15958311774706 28 24.728481714074622 27.151828592537864 21.89878093830809 26.220908755079424 29 30.767522759233216 25.34231367034268 20.953297313300272 22.936866257123825 30 29.424811474197842 24.094336832766526 24.249593375720835 22.2312583173148 31 30.481046331190427 24.695189536688094 22.81829601714328 22.005468114978203 32 31.74227870548249 24.53081129008423 23.038273976651396 20.688636027781882 33 32.01864732869616 22.96137339055794 22.369394701790736 22.650584578955158 34 25.93442380282733 24.56516912145659 28.75434830878543 20.74605876693065 35 24.049134231167677 28.636969069113512 27.586206896551722 19.727689803167088 36 26.156688839615672 28.307464892830748 23.880266075388025 21.65558019216556 37 28.830495928941524 26.173205033308662 23.83419689119171 21.162102146558105 38 27.813981042654028 27.68809241706161 22.704383886255926 21.793542654028435 39 28.736312518496597 25.22195915951465 20.864160994377034 25.177567327611722 40 22.614972466140795 19.154636106563476 23.880041672867986 34.35034975442775 41 19.304269931322782 17.982979994028067 20.155270229919378 42.55747984472977 42 22.484066992737514 26.226471024158887 24.55165258633467 26.737809396768935 43 21.057699429671874 21.5169246722465 27.146137323161245 30.279238574920374 44 23.01752301752302 22.453222453222455 25.252450252450252 29.276804276804274 45 26.173900162938825 22.952155236261294 25.129610428084725 25.744334172715156 46 23.364693681013406 27.024224016593823 25.720423735091487 23.89065856730128 47 20.52005943536404 22.25111441307578 25.63150074294205 31.597325408618126 48 25.635718509757538 22.42755765819042 27.772028385570668 24.16469544648137 49 22.839369029104645 21.261941790713173 26.29785973487373 29.600829445308452 50 23.0649696610922 24.04173449755809 28.163386118099748 24.729909723249964 51 22.1012076757798 25.39823664518041 27.81358820478625 24.68696747425354 52 21.855578573542466 25.53270198283516 28.28499556081681 24.326723882805563 53 23.677973633535775 22.559620796918974 30.165901347948456 23.5965042215968 54 21.85533427111868 22.9436588435626 30.162138150588586 25.03886873473014 55 21.687282423524184 22.213169394859637 28.501592474631508 27.597955706984667 56 22.020591067328347 23.776016591363604 29.390415524775943 24.81297681653211 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 3.5 13 14.0 14 22.0 15 21.5 16 21.0 17 30.0 18 39.0 19 34.0 20 29.0 21 26.5 22 27.0 23 30.0 24 26.5 25 23.0 26 26.5 27 30.0 28 32.5 29 43.5 30 52.0 31 56.0 32 60.0 33 67.0 34 74.0 35 87.5 36 101.0 37 127.5 38 182.5 39 211.0 40 226.0 41 241.0 42 248.0 43 255.0 44 256.0 45 257.0 46 293.0 47 394.5 48 460.0 49 586.0 50 712.0 51 720.5 52 729.0 53 695.0 54 696.5 55 732.0 56 765.0 57 798.0 58 861.5 59 925.0 60 900.5 61 876.0 62 994.0 63 1118.0 64 1124.0 65 1039.5 66 955.0 67 827.0 68 699.0 69 643.0 70 587.0 71 471.0 72 320.0 73 285.0 74 249.5 75 214.0 76 191.0 77 168.0 78 115.0 79 50.5 80 39.0 81 30.0 82 21.0 83 21.0 84 21.0 85 12.0 86 3.0 87 2.0 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4654599187292206 16 0.06649427410417437 17 0.02216475803472479 18 0.0 19 0.04432951606944958 20 0.09604728481714074 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007388252678241595 26 0.05910602142593276 27 0.04432951606944958 28 0.0 29 0.1773180642777983 30 0.06649427410417437 31 0.01477650535648319 32 0.007388252678241595 33 0.15515330624307352 34 0.1773180642777983 35 0.15515330624307352 36 0.03694126339120798 37 0.1847063169560399 38 0.22903583302548947 39 0.14037680088659032 40 0.7166605097894349 41 1.0269671222755818 42 0.30291835980790544 43 0.25120059106021425 44 0.4950129294421869 45 0.2438123383819727 46 0.2659770964166975 47 0.5541189508681197 48 0.05171776874769117 49 0.23642408570373105 50 0.15515330624307352 51 0.28075360177318065 52 0.14037680088659032 53 0.2438123383819727 54 0.20687107499076468 55 0.25120059106021425 56 0.25120059106021425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 13535.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.51200591060214 #Duplication Level Percentage of deduplicated Percentage of total 1 90.28474212034384 74.49575175471001 2 6.133595988538683 10.121906169190986 3 1.6117478510028653 3.989656446250462 4 0.770057306590258 2.541558921315109 5 0.4387535816618911 1.810121906169191 6 0.21489971346704873 1.06390838566679 7 0.10744985673352436 0.6206132249722941 8 0.08058739255014327 0.531954192833395 9 0.08954154727793696 0.6649427410417437 >10 0.2596704871060172 3.768008865903214 >50 0.008954154727793696 0.3915773919468046 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 53 0.3915773919468046 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 42 0.31030661248614705 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 41 0.30291835980790544 No Hit GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 33 0.2438123383819727 No Hit GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 28 0.20687107499076468 No Hit GTCCCGGGGGACAGGATCAACCATAGGAAAGAAGATTCCTGAGCCGGGCATGGTGA 27 0.19948282231252307 No Hit GTCCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 24 0.1773180642777983 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 22 0.1625415589213151 No Hit GTCCCGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 21 0.15515330624307352 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT 19 0.14037680088659032 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 19 0.14037680088659032 No Hit GTCCCGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 17 0.12560029553010713 No Hit GTCCCGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 16 0.11821204285186553 No Hit GTCCCGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 15 0.11082379017362395 No Hit GTCCCGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 15 0.11082379017362395 No Hit GTCCCGGGGGTTCTTTGGGATATAGCATTCTTCTCATGGTCACATGTACTGTGTAT 15 0.11082379017362395 No Hit GTCCCGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 14 0.10343553749538234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.02216475803472479 0.0 0.0 0.0 0.0 11 0.02216475803472479 0.0 0.0 0.0 0.0 12 0.02216475803472479 0.0 0.0 0.0 0.0 13 0.02216475803472479 0.0 0.0 0.0 0.0 14 0.06649427410417437 0.0 0.0 0.0 0.0 15 0.06649427410417437 0.0 0.0 0.0 0.0 16 0.06649427410417437 0.0 0.0 0.0 0.0 17 0.06649427410417437 0.0 0.0 0.0 0.0 18 0.06649427410417437 0.0 0.0 0.0 0.0 19 0.07388252678241596 0.0 0.0 0.0 0.0 20 0.07388252678241596 0.0 0.0 0.0 0.0 21 0.07388252678241596 0.0 0.0 0.0 0.0 22 0.07388252678241596 0.0 0.0 0.0 0.0 23 0.07388252678241596 0.0 0.0 0.0 0.0 24 0.09604728481714074 0.0 0.0 0.0 0.0 25 0.09604728481714074 0.0 0.0 0.0 0.0 26 0.09604728481714074 0.0 0.0 0.0 0.0 27 0.10343553749538234 0.0 0.0 0.0 0.0 28 0.12560029553010713 0.0 0.0 0.0 0.0 29 0.12560029553010713 0.0 0.0 0.0 0.0 30 0.12560029553010713 0.0 0.0 0.0 0.0 31 0.12560029553010713 0.0 0.0 0.0 0.0 32 0.12560029553010713 0.0 0.0 0.0 0.0 33 0.12560029553010713 0.0 0.0 0.0 0.0 34 0.13298854820834874 0.0 0.0 0.0 0.0 35 0.1477650535648319 0.0 0.0 0.0 0.0 36 0.1477650535648319 0.0 0.0 0.0 0.0 37 0.1477650535648319 0.0 0.0 0.0 0.0 38 0.1477650535648319 0.0 0.0 0.0 0.0 39 0.1477650535648319 0.0 0.0 0.0 0.0 40 0.1477650535648319 0.0 0.0 0.0 0.0 41 0.15515330624307352 0.0 0.0 0.0 0.0 42 0.1625415589213151 0.0 0.0 0.0 0.0 43 0.1699298115995567 0.0 0.0 0.0 0.0 44 0.1847063169560399 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCGC 20 3.89142E-4 50.37079 43 CAATCCG 20 3.89142E-4 50.37079 42 TCCGCCC 20 3.89142E-4 50.37079 45 ATCCGCC 20 3.89142E-4 50.37079 44 CACTGTC 15 0.008013309 50.370785 42 CACATCC 15 0.008133274 50.18284 13 CATCCTC 15 0.008133274 50.18284 15 ACATCCT 15 0.008133274 50.18284 14 GCAGTGG 20 3.9644205E-4 50.18284 12 AGAGCCC 15 0.008133274 50.18284 36 GGGCCCC 15 0.008133274 50.18284 9 CCGCCCA 25 1.9358307E-5 50.182835 46 AGCAGTG 25 1.9358307E-5 50.182835 11 AAGAGCC 20 4.0385048E-4 49.996284 35 GCCCAAT 15 0.00825457 49.99628 48 CCCAATC 15 0.00825457 49.99628 49 TCAACGC 20 4.113685E-4 49.811115 21 GGGAGCT 20 4.113685E-4 49.811115 8 GGGATCA 20 4.113685E-4 49.811115 7 CAACGCA 20 4.113685E-4 49.811115 22 >>END_MODULE