FastQCFastQC Report
Fri 17 Jun 2016
SRR1525953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT90.6237006237006237No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.2772002772002772No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT40.2772002772002772No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGCGTCGACTTTT30.2079002079002079No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGCCTTTT30.2079002079002079No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCCCCTTTT30.2079002079002079No Hit
GTCCCGGTGTTGTGTTTGTTTTGTTTCTTACTGACTGATA20.1386001386001386No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGCGCG20.1386001386001386No Hit
GTCCCGGGGGGTGATAAGTCTTAAATGAAAGACAGGAAAA20.1386001386001386No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC20.1386001386001386No Hit
GTCCCGGGGGTCCAAAATAAATCCCGATTTCCTTCTCATC20.1386001386001386No Hit
GTCCCGGGAGTGGTCCGCCGAGTCGACTTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGCTCGTCTTAATCAGCAACATCTTTTGATACA20.1386001386001386No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGCGTCGCCTTTT20.1386001386001386No Hit
GTCCCGGGGAGCTCATCGATTTTTTTTTTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGATGAGATGGACTTAAATGAATTGACGGGTAC20.1386001386001386No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC20.1386001386001386No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG20.1386001386001386No Hit
GTCCCGGGGATATTATTTGGTATCTTTTACTTACCTGCTT20.1386001386001386No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCCCCTTCT20.1386001386001386No Hit
GTCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGAAGCAGTGGTATCCACGCAGAGTCGACTTTT20.1386001386001386No Hit
GTCCCGGGGGGTGTGTGTCACACGTGTTTTTACTGACTGA20.1386001386001386No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG20.1386001386001386No Hit
GTCCCGGGGAGAGTGGCACGAGAGCCGACTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAAA20.1386001386001386No Hit
GTCCCGGGCATAGCATTGATTTAAAAGGAAGAGTTAACTG20.1386001386001386No Hit
GTCCCGGGGAAGATAAGTCAGGCCAGCAGAGCCTTCCTTT20.1386001386001386No Hit
GTCCCGGGGGAAGATGGGGAAGGTTTTTTTGTTTTGTTTT20.1386001386001386No Hit
GTCCCGGGGGCTCCCTCTCTTTCTCTCCTCCCTCTGCCTT20.1386001386001386No Hit
GTCCCGGGGAAGCATGGTATAACGCAGAGTCGACTTTTTT20.1386001386001386No Hit
GTCCCGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGACCTGAAGAATGTCCAGGAGTACTTCCCGGG20.1386001386001386No Hit
GTCCCGGAGTACACGAGAGTCGACTTTTTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGGGTTTTGAAAGCTTACCTGATCAGCTGGTCG20.1386001386001386No Hit
GTCCCGGGGAAGTAACGCCGATCGACTCTTTTTTTTTTTT20.1386001386001386No Hit
GTCCCGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC20.1386001386001386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG353.816731E-734.05
CCGGGGG650.034.04
CCGGGGC306.8233385E-634.04
CGGGGAA200.001945374933.9999965
GTCCCGG1500.031.7333321
TCCCGGG1450.031.6551722
CCCGGGG1350.031.4814823
CCGGGGA250.00581831427.1999994
Produced by FastQC (version 0.11.5)