##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1443
Sequences flagged as poor quality	0
Sequence length	40
%GC	56
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.956340956340956	30.0	25.0	33.0	4.0	35.0
2	23.058905058905058	26.0	17.0	31.0	4.0	34.0
3	17.777546777546778	17.0	8.0	28.0	4.0	33.0
4	16.48995148995149	15.0	10.0	24.0	4.0	33.0
5	12.927234927234927	12.0	9.0	14.0	4.0	24.0
6	20.67013167013167	23.0	9.0	30.0	4.0	33.0
7	20.076230076230075	23.0	9.0	30.0	4.0	33.0
8	20.82051282051282	25.0	6.0	31.0	4.0	33.0
9	19.81081081081081	23.0	7.0	29.0	4.0	33.0
10	18.027027027027028	19.0	4.0	27.0	4.0	32.0
11	21.62162162162162	26.0	4.0	33.0	4.0	36.0
12	21.5003465003465	25.0	4.0	33.0	4.0	36.0
13	22.435897435897434	27.0	4.0	34.0	4.0	37.0
14	22.735273735273736	28.0	4.0	34.0	4.0	37.0
15	21.395703395703396	25.0	4.0	33.0	4.0	36.0
16	20.51074151074151	24.0	4.0	33.0	4.0	36.0
17	20.814275814275813	25.0	4.0	33.0	4.0	36.0
18	20.06860706860707	23.0	4.0	33.0	4.0	36.0
19	18.04851004851005	18.0	4.0	31.0	4.0	35.0
20	19.24255024255024	22.0	4.0	33.0	4.0	36.0
21	16.752598752598754	14.0	4.0	30.0	4.0	35.0
22	15.791406791406791	10.0	4.0	29.0	4.0	34.0
23	16.133056133056133	11.0	4.0	30.0	4.0	33.0
24	15.662508662508662	4.0	4.0	29.0	4.0	34.0
25	14.66943866943867	4.0	4.0	28.0	4.0	35.0
26	12.69022869022869	4.0	4.0	24.0	4.0	33.0
27	12.271656271656271	4.0	4.0	22.0	4.0	32.0
28	11.927927927927929	4.0	4.0	22.0	4.0	32.0
29	11.592515592515593	4.0	4.0	22.0	4.0	31.0
30	10.684684684684685	4.0	4.0	17.0	4.0	31.0
31	10.194733194733194	4.0	4.0	15.0	4.0	30.0
32	9.711018711018712	4.0	4.0	11.0	4.0	30.0
33	9.387387387387387	4.0	4.0	10.0	4.0	29.0
34	9.009702009702009	4.0	4.0	4.0	4.0	28.0
35	8.705474705474705	4.0	4.0	4.0	4.0	29.0
36	8.663201663201663	4.0	4.0	4.0	4.0	30.0
37	8.43104643104643	4.0	4.0	4.0	4.0	29.0
38	8.359667359667359	4.0	4.0	4.0	4.0	30.0
39	8.113652113652114	4.0	4.0	4.0	4.0	28.0
40	7.771309771309771	4.0	4.0	4.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.7267628205128176
1	2	-10.92548076923077
1	3	-0.8782051282051277
1	4	8.185096153846153
1	5	8.920673076923077
1	6	-6.327724358974361
1	7	1.78125
1	8	-3.775641025641029
1	9	-14.6738782051282
1	10	-1.8910256410256387
1	11	2.2900641025641058
1	12	-2.701121794871799
1	13	-8.830128205128208
1	14	-2.604166666666668
1	15	-0.4639423076923066
1	16	3.5689102564102555
1	17	4.76201923076923
1	18	4.72596153846154
1	19	4.100961538461538
1	20	13.701121794871796
1	21	16.708333333333332
1	22	-3.6490384615384617
1	23	6.7724358974359
1	24	10.869391025641024
1	25	18.471153846153847
1	26	14.621794871794872
1	27	17.46955128205128
1	28	20.548878205128204
1	29	17.82772435897436
1	30	-0.2844551282051295
1	31	16.51842948717949
1	32	21.621794871794872
1	33	-5.288461538461538
1	34	-4.5368589743589745
1	35	-4.397435897435898
1	36	-4.398237179487181
1	37	-3.90625
1	38	-3.478365384615385
1	39	-3.346153846153846
1	40	-3.292467948717948
3	1	-9.773237179487182
3	2	-4.42548076923077
3	3	-10.378205128205128
3	4	-7.314903846153847
3	5	-6.079326923076923
3	6	-1.8277243589743613
3	7	-3.21875
3	8	-4.775641025641029
3	9	-4.673878205128201
3	10	-2.3910256410256387
3	11	0.29006410256410575
3	12	0.29887820512820085
3	13	-1.830128205128208
3	14	-3.104166666666668
3	15	0.5360576923076934
3	16	2.0689102564102555
3	17	-1.23798076923077
3	18	2.22596153846154
3	19	4.100961538461538
3	20	0.2011217948717956
3	21	4.208333333333332
3	22	-0.14903846153846168
3	23	5.2724358974359
3	24	5.369391025641024
3	25	6.971153846153847
3	26	8.621794871794872
3	27	8.469551282051281
3	28	3.0488782051282044
3	29	7.327724358974361
3	30	6.2155448717948705
3	31	5.018429487179487
3	32	4.121794871794872
3	33	4.711538461538462
3	34	8.963141025641026
3	35	5.102564102564102
3	36	9.10176282051282
3	37	-3.90625
3	38	-3.478365384615385
3	39	-3.346153846153846
3	40	-3.292467948717948
4	1	-9.773237179487182
4	2	-17.92548076923077
4	3	-14.878205128205128
4	4	-13.814903846153847
4	5	-9.079326923076923
4	6	-16.32772435897436
4	7	-16.21875
4	8	-16.77564102564103
4	9	-16.6738782051282
4	10	-13.891025641025639
4	11	-16.709935897435894
4	12	-16.7011217948718
4	13	-18.830128205128208
4	14	-18.604166666666668
4	15	-16.463942307692307
4	16	-14.431089743589745
4	17	-15.23798076923077
4	18	-14.27403846153846
4	19	-11.899038461538462
4	20	-13.298878205128204
4	21	-11.291666666666668
4	22	-9.649038461538462
4	23	-10.2275641025641
4	24	-9.130608974358976
4	25	-8.528846153846153
4	26	-7.378205128205128
4	27	-7.530448717948719
4	28	-6.451121794871796
4	29	-6.172275641025639
4	30	-6.2844551282051295
4	31	-5.481570512820513
4	32	-5.378205128205128
4	33	-5.288461538461538
4	34	-4.5368589743589745
4	35	-4.397435897435898
4	36	-4.398237179487181
4	37	-3.90625
4	38	-3.478365384615385
4	39	-3.346153846153846
4	40	-3.292467948717948
5	1	3.2267628205128176
5	2	7.07451923076923
5	3	-5.878205128205128
5	4	-3.3149038461538467
5	5	18.420673076923077
5	6	3.6722756410256387
5	7	-0.71875
5	8	-0.7756410256410291
5	9	-13.1738782051282
5	10	3.1089743589743613
5	11	-0.20993589743589425
5	12	-0.20112179487179915
5	13	4.669871794871792
5	14	5.895833333333332
5	15	-3.9639423076923066
5	16	-2.4310897435897445
5	17	7.26201923076923
5	18	4.22596153846154
5	19	3.6009615384615383
5	20	4.201121794871796
5	21	16.208333333333332
5	22	0.3509615384615383
5	23	7.7724358974359
5	24	5.869391025641024
5	25	6.471153846153847
5	26	18.621794871794872
5	27	5.469551282051281
5	28	-0.9511217948717956
5	29	3.3277243589743613
5	30	8.21554487179487
5	31	5.518429487179487
5	32	10.621794871794872
5	33	0.21153846153846168
5	34	10.963141025641026
5	35	-4.397435897435898
5	36	-4.398237179487181
5	37	-3.90625
5	38	-3.478365384615385
5	39	-3.346153846153846
5	40	-3.292467948717948
6	1	-8.773237179487182
6	2	-8.92548076923077
6	3	-3.3782051282051277
6	4	-7.814903846153847
6	5	2.9206730769230766
6	6	-0.8277243589743613
6	7	-0.71875
6	8	-1.275641025641029
6	9	-1.1738782051282008
6	10	1.6089743589743613
6	11	-4.209935897435894
6	12	-2.201121794871799
6	13	-2.330128205128208
6	14	-3.604166666666668
6	15	0.0360576923076934
6	16	-4.9310897435897445
6	17	-0.73798076923077
6	18	1.22596153846154
6	19	2.6009615384615383
6	20	1.2011217948717956
6	21	2.708333333333332
6	22	0.3509615384615383
6	23	5.2724358974359
6	24	7.869391025641024
6	25	8.471153846153847
6	26	5.121794871794872
6	27	5.969551282051281
6	28	6.548878205128204
6	29	-6.172275641025639
6	30	-6.2844551282051295
6	31	-5.481570512820513
6	32	-5.378205128205128
6	33	-5.288461538461538
6	34	-4.5368589743589745
6	35	-4.397435897435898
6	36	-4.398237179487181
6	37	-3.90625
6	38	-3.478365384615385
6	39	-3.346153846153846
6	40	-3.292467948717948
7	1	7.226762820512818
7	2	12.57451923076923
7	3	12.121794871794872
7	4	11.685096153846153
7	5	1.4206730769230766
7	6	10.672275641025639
7	7	10.28125
7	8	4.224358974358971
7	9	11.3261217948718
7	10	15.608974358974361
7	11	4.790064102564106
7	12	4.298878205128201
7	13	9.169871794871792
7	14	12.895833333333332
7	15	8.536057692307693
7	16	8.068910256410255
7	17	11.76201923076923
7	18	-0.7740384615384599
7	19	17.60096153846154
7	20	14.701121794871796
7	21	9.208333333333332
7	22	21.85096153846154
7	23	13.2724358974359
7	24	22.369391025641022
7	25	14.971153846153847
7	26	17.621794871794872
7	27	12.469551282051281
7	28	12.548878205128204
7	29	4.327724358974361
7	30	19.21554487179487
7	31	11.018429487179487
7	32	7.621794871794872
7	33	-2.2884615384615383
7	34	7.9631410256410255
7	35	2.602564102564102
7	36	2.1017628205128194
7	37	6.59375
7	38	-0.478365384615385
7	39	2.653846153846154
7	40	11.207532051282051
8	1	-10.273237179487182
8	2	-4.92548076923077
8	3	-1.8782051282051277
8	4	-13.814903846153847
8	5	-9.079326923076923
8	6	-16.32772435897436
8	7	-16.21875
8	8	-16.77564102564103
8	9	-16.6738782051282
8	10	-13.891025641025639
8	11	-16.709935897435894
8	12	-16.7011217948718
8	13	-18.830128205128208
8	14	-18.604166666666668
8	15	-16.463942307692307
8	16	-14.431089743589745
8	17	-15.23798076923077
8	18	-14.27403846153846
8	19	-11.899038461538462
8	20	-13.298878205128204
8	21	-11.291666666666668
8	22	-9.649038461538462
8	23	-10.2275641025641
8	24	-9.130608974358976
8	25	-8.528846153846153
8	26	-7.378205128205128
8	27	-7.530448717948719
8	28	-6.451121794871796
8	29	-6.172275641025639
8	30	-6.2844551282051295
8	31	-5.481570512820513
8	32	-5.378205128205128
8	33	-5.288461538461538
8	34	-4.5368589743589745
8	35	-4.397435897435898
8	36	-4.398237179487181
8	37	-3.90625
8	38	-3.478365384615385
8	39	-3.346153846153846
8	40	-3.292467948717948
9	1	0.4767628205128176
9	2	-0.17548076923077005
9	3	7.621794871794872
9	4	4.935096153846153
9	5	-0.8293269230769234
9	6	0.9222756410256387
9	7	5.53125
9	8	5.724358974358971
9	9	4.576121794871799
9	10	-5.891025641025639
9	11	8.290064102564106
9	12	5.548878205128201
9	13	10.669871794871792
9	14	2.395833333333332
9	15	4.786057692307693
9	16	-2.4310897435897445
9	17	6.01201923076923
9	18	6.22596153846154
9	19	-0.8990384615384617
9	20	3.9511217948717956
9	21	4.208333333333332
9	22	6.350961538461538
9	23	4.7724358974359
9	24	5.619391025641024
9	25	6.971153846153847
9	26	-0.37820512820512775
9	27	-0.03044871794871895
9	28	-1.7011217948717956
9	29	-4.922275641025639
9	30	-0.034455128205129526
9	31	-2.7315705128205128
9	32	-3.8782051282051277
9	33	1.7115384615384617
9	34	1.4631410256410255
9	35	0.6025641025641022
9	36	2.3517628205128194
9	37	0.84375
9	38	-0.728365384615385
9	39	-0.3461538461538458
9	40	3.457532051282052
10	1	4.06009615384615
10	2	7.07451923076923
10	3	-4.544871794871794
10	4	14.518429487179489
10	5	-0.07932692307692335
10	6	9.005608974358971
10	7	7.114583333333332
10	8	9.224358974358971
10	9	3.326121794871799
10	10	7.108974358974361
10	11	1.9567307692307736
10	12	8.965544871794869
10	13	11.503205128205128
10	14	10.395833333333332
10	15	8.202724358974361
10	16	13.568910256410255
10	17	12.095352564102562
10	18	17.059294871794876
10	19	19.434294871794876
10	20	15.701121794871796
10	21	22.041666666666668
10	22	23.684294871794876
10	23	21.439102564102562
10	24	22.202724358974358
10	25	24.13782051282051
10	26	19.955128205128204
10	27	20.46955128205128
10	28	14.215544871794872
10	29	19.161057692307693
10	30	10.048878205128203
10	31	19.185096153846153
10	32	15.28846153846154
10	33	15.37820512820513
10	34	8.129807692307693
10	35	16.26923076923077
10	36	12.268429487179487
10	37	21.09375
10	38	17.188301282051285
10	39	18.653846153846153
10	40	18.040865384615383
11	1	6.226762820512818
11	2	3.57451923076923
11	3	14.621794871794872
11	4	12.185096153846153
11	5	17.420673076923077
11	6	13.172275641025639
11	7	15.78125
11	8	13.224358974358971
11	9	15.3261217948718
11	10	15.108974358974361
11	11	9.290064102564106
11	12	8.7988782051282
11	13	9.169871794871792
11	14	11.895833333333332
11	15	5.536057692307693
11	16	6.5689102564102555
11	17	11.26201923076923
11	18	12.22596153846154
11	19	2.6009615384615383
11	20	14.701121794871796
11	21	8.708333333333332
11	22	11.350961538461538
11	23	8.2724358974359
11	24	11.369391025641024
11	25	11.471153846153847
11	26	23.621794871794872
11	27	8.969551282051281
11	28	19.548878205128204
11	29	13.827724358974361
11	30	18.71554487179487
11	31	21.01842948717949
11	32	20.121794871794872
11	33	24.71153846153846
11	34	23.963141025641026
11	35	8.602564102564102
11	36	-4.398237179487181
11	37	-3.90625
11	38	-3.478365384615385
11	39	-3.346153846153846
11	40	-3.292467948717948
12	1	-8.273237179487182
12	2	-3.592147435897438
12	3	-11.21153846153846
12	4	-8.481570512820513
12	5	-3.7459935897435894
12	6	-6.994391025641027
12	7	-6.885416666666666
12	8	-2.442307692307697
12	9	-4.673878205128201
12	10	0.7756410256410291
12	11	-1.0432692307692264
12	12	3.9655448717948687
12	13	0.8365384615384599
12	14	-0.2708333333333357
12	15	1.5360576923076934
12	16	8.235576923076923
12	17	4.76201923076923
12	18	8.392628205128208
12	19	7.4342948717948705
12	20	9.367788461538463
12	21	8.041666666666664
12	22	10.350961538461538
12	23	5.7724358974359
12	24	11.536057692307692
12	25	12.471153846153847
12	26	6.955128205128204
12	27	9.802884615384613
12	28	9.548878205128204
12	29	11.827724358974361
12	30	13.048878205128203
12	31	7.185096153846155
12	32	7.621794871794872
12	33	2.0448717948717956
12	34	-1.5368589743589745
12	35	-0.3974358974358978
12	36	4.2684294871794854
12	37	4.760416666666666
12	38	-3.478365384615385
12	39	-3.346153846153846
12	40	-3.292467948717948
13	1	2.7267628205128176
13	2	-17.92548076923077
13	3	-14.878205128205128
13	4	-13.814903846153847
13	5	-9.079326923076923
13	6	-16.32772435897436
13	7	-16.21875
13	8	-16.77564102564103
13	9	-16.6738782051282
13	10	-13.891025641025639
13	11	-16.709935897435894
13	12	-16.7011217948718
13	13	-18.830128205128208
13	14	-18.604166666666668
13	15	-16.463942307692307
13	16	-14.431089743589745
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111	24	-9.130608974358976
111	25	-8.528846153846153
111	26	-7.378205128205128
111	27	-7.530448717948719
111	28	-6.451121794871796
111	29	-6.172275641025639
111	30	-6.2844551282051295
111	31	-5.481570512820513
111	32	-5.378205128205128
111	33	-5.288461538461538
111	34	-4.5368589743589745
111	35	-4.397435897435898
111	36	-4.398237179487181
111	37	-3.90625
111	38	-3.478365384615385
111	39	-3.346153846153846
111	40	-3.292467948717948
112	1	-12.273237179487182
112	2	-14.42548076923077
112	3	-3.8782051282051277
112	4	-2.3149038461538467
112	5	-5.079326923076923
112	6	-4.827724358974361
112	7	-7.71875
112	8	-7.275641025641029
112	9	-6.173878205128201
112	10	-4.891025641025639
112	11	-7.709935897435894
112	12	-5.201121794871799
112	13	-7.330128205128208
112	14	-5.604166666666668
112	15	-5.463942307692307
112	16	-10.931089743589745
112	17	-1.73798076923077
112	18	-11.27403846153846
112	19	-1.3990384615384617
112	20	-1.2988782051282044
112	21	-5.791666666666668
112	22	-1.6490384615384617
112	23	-8.7275641025641
112	24	-2.6306089743589762
112	25	0.4711538461538467
112	26	1.6217948717948723
112	27	-4.030448717948719
112	28	-0.9511217948717956
112	29	-0.6722756410256387
112	30	0.7155448717948705
112	31	3.5184294871794872
112	32	4.621794871794872
112	33	2.2115384615384617
112	34	4.4631410256410255
112	35	3.102564102564102
112	36	10.60176282051282
112	37	9.09375
112	38	8.521634615384615
112	39	8.653846153846153
112	40	6.207532051282052
113	1	2.7267628205128176
113	2	-1.92548076923077
113	3	-7.878205128205128
113	4	-6.814903846153847
113	5	-4.079326923076923
113	6	4.672275641025639
113	7	0.78125
113	8	-1.775641025641029
113	9	2.326121794871799
113	10	8.108974358974361
113	11	12.290064102564106
113	12	8.2988782051282
113	13	13.169871794871792
113	14	12.395833333333332
113	15	-2.4639423076923066
113	16	4.5689102564102555
113	17	8.76201923076923
113	18	2.72596153846154
113	19	15.100961538461538
113	20	-13.298878205128204
113	21	-11.291666666666668
113	22	-9.649038461538462
113	23	-10.2275641025641
113	24	-9.130608974358976
113	25	-8.528846153846153
113	26	-7.378205128205128
113	27	-7.530448717948719
113	28	-6.451121794871796
113	29	-6.172275641025639
113	30	-6.2844551282051295
113	31	-5.481570512820513
113	32	-5.378205128205128
113	33	-5.288461538461538
113	34	-4.5368589743589745
113	35	-4.397435897435898
113	36	-4.398237179487181
113	37	-3.90625
113	38	-3.478365384615385
113	39	-3.346153846153846
113	40	-3.292467948717948
114	1	-9.773237179487182
114	2	-6.92548076923077
114	3	-11.878205128205128
114	4	-10.314903846153847
114	5	-4.579326923076923
114	6	-4.327724358974361
114	7	-2.71875
114	8	-8.275641025641029
114	9	-5.173878205128201
114	10	-11.891025641025639
114	11	-5.209935897435894
114	12	-5.201121794871799
114	13	-12.330128205128208
114	14	-5.104166666666668
114	15	-9.963942307692307
114	16	-4.4310897435897445
114	17	-5.23798076923077
114	18	0.2259615384615401
114	19	-4.899038461538462
114	20	-0.2988782051282044
114	21	-4.291666666666668
114	22	6.350961538461538
114	23	2.7724358974358996
114	24	6.369391025641024
114	25	6.471153846153847
114	26	4.621794871794872
114	27	1.469551282051281
114	28	2.0488782051282044
114	29	-0.6722756410256387
114	30	-0.7844551282051295
114	31	4.518429487179487
114	32	4.621794871794872
114	33	7.211538461538462
114	34	7.4631410256410255
114	35	4.102564102564102
114	36	5.601762820512819
114	37	11.59375
114	38	12.021634615384615
114	39	2.153846153846154
114	40	12.207532051282051
115	1	-6.273237179487182
115	2	4.57451923076923
115	3	-10.878205128205128
115	4	-9.814903846153847
115	5	-6.079326923076923
115	6	-2.8277243589743613
115	7	-5.21875
115	8	-2.775641025641029
115	9	-6.673878205128201
115	10	-1.8910256410256387
115	11	-4.709935897435894
115	12	-4.201121794871799
115	13	-7.330128205128208
115	14	-3.604166666666668
115	15	-11.463942307692307
115	16	1.5689102564102555
115	17	-0.73798076923077
115	18	1.72596153846154
115	19	4.100961538461538
115	20	2.2011217948717956
115	21	5.208333333333332
115	22	6.850961538461538
115	23	6.2724358974359
115	24	5.369391025641024
115	25	7.471153846153847
115	26	-3.3782051282051277
115	27	-2.030448717948719
115	28	-2.4511217948717956
115	29	3.3277243589743613
115	30	2.2155448717948705
115	31	2.5184294871794872
115	32	3.1217948717948723
115	33	0.7115384615384617
115	34	5.9631410256410255
115	35	1.6025641025641022
115	36	11.10176282051282
115	37	11.59375
115	38	12.021634615384615
115	39	5.653846153846154
115	40	12.207532051282051
116	1	2.7267628205128176
116	2	-17.92548076923077
116	3	-14.878205128205128
116	4	-13.814903846153847
116	5	-9.079326923076923
116	6	-16.32772435897436
116	7	-16.21875
116	8	-16.77564102564103
116	9	-16.6738782051282
116	10	-13.891025641025639
116	11	-16.709935897435894
116	12	-16.7011217948718
116	13	-18.830128205128208
116	14	-18.604166666666668
116	15	-16.463942307692307
116	16	-14.431089743589745
116	17	-15.23798076923077
116	18	-14.27403846153846
116	19	-11.899038461538462
116	20	-13.298878205128204
116	21	-11.291666666666668
116	22	-9.649038461538462
116	23	-10.2275641025641
116	24	-9.130608974358976
116	25	-8.528846153846153
116	26	-7.378205128205128
116	27	-7.530448717948719
116	28	-6.451121794871796
116	29	-6.172275641025639
116	30	-6.2844551282051295
116	31	-5.481570512820513
116	32	-5.378205128205128
116	33	-5.288461538461538
116	34	-4.5368589743589745
116	35	-4.397435897435898
116	36	-4.398237179487181
116	37	-3.90625
116	38	-3.478365384615385
116	39	-3.346153846153846
116	40	-3.292467948717948
117	1	5.726762820512818
117	2	11.07451923076923
117	3	15.121794871794872
117	4	-3.8149038461538467
117	5	4.920673076923077
117	6	7.672275641025639
117	7	12.78125
117	8	-1.775641025641029
117	9	6.326121794871799
117	10	10.108974358974361
117	11	15.290064102564106
117	12	9.2988782051282
117	13	12.169871794871792
117	14	13.395833333333332
117	15	14.536057692307693
117	16	-0.4310897435897445
117	17	13.76201923076923
117	18	14.72596153846154
117	19	17.10096153846154
117	20	17.701121794871796
117	21	11.708333333333332
117	22	-2.6490384615384617
117	23	18.772435897435898
117	24	-9.130608974358976
117	25	-8.528846153846153
117	26	-7.378205128205128
117	27	-7.530448717948719
117	28	-6.451121794871796
117	29	-6.172275641025639
117	30	-6.2844551282051295
117	31	-5.481570512820513
117	32	-5.378205128205128
117	33	-5.288461538461538
117	34	-4.5368589743589745
117	35	-4.397435897435898
117	36	-4.398237179487181
117	37	-3.90625
117	38	-3.478365384615385
117	39	-3.346153846153846
117	40	-3.292467948717948
118	1	5.726762820512818
118	2	11.07451923076923
118	3	-14.878205128205128
118	4	-13.814903846153847
118	5	-9.079326923076923
118	6	-16.32772435897436
118	7	-16.21875
118	8	-16.77564102564103
118	9	-16.6738782051282
118	10	-13.891025641025639
118	11	-16.709935897435894
118	12	-16.7011217948718
118	13	-18.830128205128208
118	14	-18.604166666666668
118	15	-16.463942307692307
118	16	-14.431089743589745
118	17	-15.23798076923077
118	18	-14.27403846153846
118	19	-11.899038461538462
118	20	-13.298878205128204
118	21	-11.291666666666668
118	22	-9.649038461538462
118	23	-10.2275641025641
118	24	-9.130608974358976
118	25	-8.528846153846153
118	26	-7.378205128205128
118	27	-7.530448717948719
118	28	-6.451121794871796
118	29	-6.172275641025639
118	30	-6.2844551282051295
118	31	-5.481570512820513
118	32	-5.378205128205128
118	33	-5.288461538461538
118	34	-4.5368589743589745
118	35	-4.397435897435898
118	36	-4.398237179487181
118	37	-3.90625
118	38	-3.478365384615385
118	39	-3.346153846153846
118	40	-3.292467948717948
119	1	3.7267628205128176
119	2	-17.92548076923077
119	3	-14.878205128205128
119	4	-13.814903846153847
119	5	-9.079326923076923
119	6	-16.32772435897436
119	7	-16.21875
119	8	-16.77564102564103
119	9	-16.6738782051282
119	10	-13.891025641025639
119	11	-16.709935897435894
119	12	-16.7011217948718
119	13	-18.830128205128208
119	14	-18.604166666666668
119	15	-16.463942307692307
119	16	-14.431089743589745
119	17	-15.23798076923077
119	18	-14.27403846153846
119	19	-11.899038461538462
119	20	-13.298878205128204
119	21	-11.291666666666668
119	22	-9.649038461538462
119	23	-10.2275641025641
119	24	-9.130608974358976
119	25	-8.528846153846153
119	26	-7.378205128205128
119	27	-7.530448717948719
119	28	-6.451121794871796
119	29	-6.172275641025639
119	30	-6.2844551282051295
119	31	-5.481570512820513
119	32	-5.378205128205128
119	33	-5.288461538461538
119	34	-4.5368589743589745
119	35	-4.397435897435898
119	36	-4.398237179487181
119	37	-3.90625
119	38	-3.478365384615385
119	39	-3.346153846153846
119	40	-3.292467948717948
120	1	-23.273237179487182
120	2	-17.92548076923077
120	3	-14.878205128205128
120	4	-13.814903846153847
120	5	-9.079326923076923
120	6	-16.32772435897436
120	7	-16.21875
120	8	-16.77564102564103
120	9	-16.6738782051282
120	10	-13.891025641025639
120	11	-16.709935897435894
120	12	-16.7011217948718
120	13	-18.830128205128208
120	14	-18.604166666666668
120	15	-16.463942307692307
120	16	-14.431089743589745
120	17	-15.23798076923077
120	18	-14.27403846153846
120	19	-11.899038461538462
120	20	-13.298878205128204
120	21	-11.291666666666668
120	22	-9.649038461538462
120	23	-10.2275641025641
120	24	-9.130608974358976
120	25	-8.528846153846153
120	26	-7.378205128205128
120	27	-7.530448717948719
120	28	-6.451121794871796
120	29	-6.172275641025639
120	30	-6.2844551282051295
120	31	-5.481570512820513
120	32	-5.378205128205128
120	33	-5.288461538461538
120	34	-4.5368589743589745
120	35	-4.397435897435898
120	36	-4.398237179487181
120	37	-3.90625
120	38	-3.478365384615385
120	39	-3.346153846153846
120	40	-3.292467948717948
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	301.0
5	37.0
6	6.0
7	4.0
8	7.0
9	15.0
10	21.0
11	39.0
12	49.0
13	67.0
14	58.0
15	63.0
16	79.0
17	55.0
18	73.0
19	86.0
20	64.0
21	53.0
22	81.0
23	64.0
24	51.0
25	33.0
26	41.0
27	31.0
28	23.0
29	18.0
30	14.0
31	6.0
32	2.0
33	1.0
34	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.47539847539848	1.0395010395010396	0.2772002772002772	0.2079002079002079
8	95.76976421636616	1.3176144244105408	1.0402219140083218	1.872399445214979
9	90.83969465648855	4.9271339347675225	1.457321304649549	2.7758501040943786
10	35.2044352044352	39.84753984753985	9.702009702009702	15.246015246015245
11	27.650727650727653	34.303534303534306	20.097020097020096	17.94871794871795
12	40.60984060984061	16.97851697851698	19.196119196119195	23.215523215523216
13	22.607489597780862	17.683772538141472	25.104022191400833	34.60471567267684
14	23.839223839223838	30.561330561330564	23.90852390852391	21.69092169092169
15	25.502425502425503	25.571725571725572	25.64102564102564	23.284823284823286
16	26.47262647262647	24.80942480942481	30.6999306999307	18.01801801801802
17	38.80803880803881	20.85932085932086	20.304920304920305	20.02772002772003
18	36.10533610533611	23.839223839223838	18.433818433818434	21.62162162162162
19	20.361362056984017	20.013898540653233	22.6546212647672	36.970118137595556
20	22.106722106722106	36.03603603603604	19.81981981981982	22.03742203742204
21	18.711018711018713	19.542619542619544	37.56063756063756	24.185724185724187
22	23.007623007623007	17.6022176022176	22.66112266112266	36.72903672903673
23	21.136521136521136	30.14553014553015	23.215523215523216	25.502425502425503
24	19.68121968121968	32.155232155232156	22.106722106722106	26.056826056826054
25	19.36155447605829	28.868841082581543	20.8882720333102	30.881332408049968
26	23.467966573816156	23.050139275766014	26.81058495821727	26.67130919220056
27	21.8988218988219	21.067221067221066	25.363825363825367	31.670131670131667
28	19.95841995841996	28.62092862092862	24.393624393624393	27.027027027027028
29	22.01388888888889	23.819444444444443	28.333333333333332	25.833333333333336
30	19.166666666666668	22.5	29.583333333333332	28.749999999999996
31	19.791666666666664	22.5	35.0	22.708333333333332
32	17.87941787941788	19.88911988911989	35.2044352044352	27.027027027027028
33	15.86971586971587	22.73042273042273	32.84823284823285	28.55162855162855
34	16.354816354816354	22.176022176022176	36.72903672903673	24.74012474012474
35	12.430555555555555	25.972222222222225	34.375	27.22222222222222
36	14.166666666666666	24.930555555555557	34.375	26.52777777777778
37	14.50381679389313	22.970159611380986	36.43303261623873	26.092990978487162
38	12.82051282051282	21.968121968121967	36.24393624393624	28.967428967428965
39	12.552011095700417	23.231622746185852	35.714285714285715	28.502080443828014
40	13.374913374913374	23.562023562023562	37.83783783783784	25.225225225225223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	0.5
20	0.0
21	1.5
22	3.0
23	3.0
24	5.0
25	7.0
26	13.0
27	19.0
28	19.0
29	17.5
30	16.0
31	18.5
32	21.0
33	21.0
34	22.0
35	23.0
36	27.0
37	31.0
38	31.0
39	37.5
40	44.0
41	48.5
42	53.0
43	53.0
44	53.5
45	54.0
46	65.5
47	77.0
48	77.0
49	83.0
50	89.0
51	93.0
52	97.0
53	97.0
54	107.0
55	117.0
56	129.5
57	142.0
58	142.0
59	121.5
60	101.0
61	105.5
62	110.0
63	110.0
64	102.5
65	95.0
66	96.0
67	97.0
68	97.0
69	90.0
70	83.0
71	77.0
72	71.0
73	71.0
74	57.5
75	44.0
76	35.0
77	26.0
78	26.0
79	19.0
80	12.0
81	10.0
82	8.0
83	8.0
84	4.5
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0693000693000693
9	0.1386001386001386
10	0.0
11	0.0
12	0.0
13	0.0693000693000693
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.2772002772002772
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.1386001386001386
26	0.4851004851004851
27	0.0
28	0.0
29	0.2079002079002079
30	0.2079002079002079
31	0.2079002079002079
32	0.0
33	0.0
34	0.0
35	0.2079002079002079
36	0.2079002079002079
37	0.1386001386001386
38	0.0
39	0.0693000693000693
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1443.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.46569646569647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.3419540229885	93.9015939015939
2	2.227011494252874	4.296604296604296
3	0.21551724137931033	0.6237006237006237
4	0.14367816091954022	0.5544005544005544
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.07183908045977011	0.6237006237006237
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGAAGCAGTGGTATCAACGCCGAGTCGACTTTT	9	0.6237006237006237	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.2772002772002772	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	4	0.2772002772002772	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGCGTCGACTTTT	3	0.2079002079002079	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGCCTTTT	3	0.2079002079002079	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCCCCTTTT	3	0.2079002079002079	No Hit
GTCCCGGTGTTGTGTTTGTTTTGTTTCTTACTGACTGATA	2	0.1386001386001386	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGCGCG	2	0.1386001386001386	No Hit
GTCCCGGGGGGTGATAAGTCTTAAATGAAAGACAGGAAAA	2	0.1386001386001386	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	2	0.1386001386001386	No Hit
GTCCCGGGGGTCCAAAATAAATCCCGATTTCCTTCTCATC	2	0.1386001386001386	No Hit
GTCCCGGGAGTGGTCCGCCGAGTCGACTTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	2	0.1386001386001386	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCCGCGTCGCCTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGAGCTCATCGATTTTTTTTTTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGATGAGATGGACTTAAATGAATTGACGGGTAC	2	0.1386001386001386	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	2	0.1386001386001386	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG	2	0.1386001386001386	No Hit
GTCCCGGGGATATTATTTGGTATCTTTTACTTACCTGCTT	2	0.1386001386001386	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCCCCTTCT	2	0.1386001386001386	No Hit
GTCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGAAGCAGTGGTATCCACGCAGAGTCGACTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGGGTGTGTGTCACACGTGTTTTTACTGACTGA	2	0.1386001386001386	No Hit
GTCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG	2	0.1386001386001386	No Hit
GTCCCGGGGAGAGTGGCACGAGAGCCGACTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAAA	2	0.1386001386001386	No Hit
GTCCCGGGCATAGCATTGATTTAAAAGGAAGAGTTAACTG	2	0.1386001386001386	No Hit
GTCCCGGGGAAGATAAGTCAGGCCAGCAGAGCCTTCCTTT	2	0.1386001386001386	No Hit
GTCCCGGGGGAAGATGGGGAAGGTTTTTTTGTTTTGTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGGCTCCCTCTCTTTCTCTCCTCCCTCTGCCTT	2	0.1386001386001386	No Hit
GTCCCGGGGAAGCATGGTATAACGCAGAGTCGACTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGACCTGAAGAATGTCCAGGAGTACTTCCCGGG	2	0.1386001386001386	No Hit
GTCCCGGAGTACACGAGAGTCGACTTTTTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGGGTTTTGAAAGCTTACCTGATCAGCTGGTCG	2	0.1386001386001386	No Hit
GTCCCGGGGAAGTAACGCCGATCGACTCTTTTTTTTTTTT	2	0.1386001386001386	No Hit
GTCCCGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	2	0.1386001386001386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0693000693000693	0.0	0.0	0.0	0.0
12	0.0693000693000693	0.0	0.0	0.0	0.0
13	0.0693000693000693	0.0	0.0	0.0	0.0
14	0.0693000693000693	0.0	0.0	0.0	0.0
15	0.0693000693000693	0.0	0.0	0.0	0.0
16	0.1386001386001386	0.0	0.0	0.0	0.0
17	0.1386001386001386	0.0	0.0	0.0	0.0
18	0.1386001386001386	0.0	0.0	0.0	0.0
19	0.1386001386001386	0.0	0.0	0.0	0.0
20	0.1386001386001386	0.0	0.0	0.0	0.0
21	0.1386001386001386	0.0	0.0	0.0	0.0
22	0.1386001386001386	0.0	0.0	0.0	0.0
23	0.1386001386001386	0.0	0.0	0.0	0.0
24	0.1386001386001386	0.0	0.0	0.0	0.0
25	0.1386001386001386	0.0	0.0	0.0	0.0
26	0.1386001386001386	0.0	0.0	0.0	0.0
27	0.1386001386001386	0.0	0.0	0.0	0.0
28	0.1386001386001386	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	35	3.816731E-7	34.0	5
CCGGGGG	65	0.0	34.0	4
CCGGGGC	30	6.8233385E-6	34.0	4
CGGGGAA	20	0.0019453749	33.999996	5
GTCCCGG	150	0.0	31.733332	1
TCCCGGG	145	0.0	31.655172	2
CCCGGGG	135	0.0	31.481482	3
CCGGGGA	25	0.005818314	27.199999	4
>>END_MODULE