FastQCFastQC Report
Fri 17 Jun 2016
SRR1525951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC100.7401924500370096No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT100.7401924500370096No Hit
GTCCCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT80.5921539600296076No Hit
GTCCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.44411547002220575No Hit
GTCCCGGGGGTTCTTTGGGATATAGCATTCTTCTCATGGT40.2960769800148038No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAGC40.2960769800148038No Hit
GTCCCGGGGATTTCCACTATGTTCTATCAATGGGAGCAGT40.2960769800148038No Hit
GTCCCGGGGTGGGGGCTTGGTAGCCCTTTCTGCTAGGGAC30.22205773501110287No Hit
GTCCCGGGGATAGCAGGATTTGTTCACTGATTCCCATTAT30.22205773501110287No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC30.22205773501110287No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.22205773501110287No Hit
GTCCCGGGGATCGTTTTAAATGAGAATGCAAAATAAGGTA30.22205773501110287No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG30.22205773501110287No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA30.22205773501110287No Hit
GTCCCGGGGATTTTATGGATGCTAGTCATCTTGAAAGATT30.22205773501110287No Hit
GTCCCGGGGCATGGATTACAGTTTTTTAAAAACTAGAATT30.22205773501110287No Hit
GTCCCGGGGATTTTCATGAGATACTCAGTTTTGTCCTCCA20.1480384900074019No Hit
GTCCCGGGGATTGCTGGTGGCTCTAAGATGCAGTATAATT20.1480384900074019No Hit
GTCCCGGGGGTGTGGGGGATCTATGGCCATCTCCTACCAT20.1480384900074019No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGC20.1480384900074019No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGA20.1480384900074019No Hit
GTCCCGGGGGTTTTAAGAAGATATATTCCTATAGTACACT20.1480384900074019No Hit
GTCCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1480384900074019No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTA20.1480384900074019No Hit
GTCCCGGGGGAGGTGATCAAGCTGAAAGGTTACACATCCT20.1480384900074019No Hit
GTCCCGGGGCTATAGTATAAGTTTGAAATTTCGGTTGGGG20.1480384900074019No Hit
GTCCCGGGGATCTGGGTCTTCAGTACTCTGGGAAACAGAT20.1480384900074019No Hit
GTCCCGGGGGACCCGGGGAGGTAGTGACGAAAAATAACAA20.1480384900074019No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC20.1480384900074019No Hit
GTCCCGGGGGGTTGTATTCGATGTTCTCTGTTTAGGCCCA20.1480384900074019No Hit
GTCCCGGGGATTCTTGCTCATGTGGGACCAACGGCCAGTA20.1480384900074019No Hit
GTCCCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG20.1480384900074019No Hit
GTCCCGGGGTCATGAATGGCCATTCTACCCAGATAGGTTT20.1480384900074019No Hit
GTCCCGGGGGTCTTCAAAGTCAAATCTTACACGGTCTTCA20.1480384900074019No Hit
GTCCCGGGGGGGCTGGAGAGGTGGCTCAGCAGATAAGAGC20.1480384900074019No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.1480384900074019No Hit
GTCCCGGGGCTGGGTAGTGATTTCAGTCATCTCAGAAGAC20.1480384900074019No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCACTGGGTAAGAGC20.1480384900074019No Hit
GTCCCGGGGGATCCAAAAAGATAAAAGGAACTCGGCAAAC20.1480384900074019No Hit
GTCCCGGGGGTCTGGTTCCCAGGATAAGACTGTTAGATTC20.1480384900074019No Hit
GTCCCGGGGGGTTTTACTATTATTTGTTTTCAATGTATAA20.1480384900074019No Hit
GTCCCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1480384900074019No Hit
GTCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT20.1480384900074019No Hit
GTCCCGGGGGGATTTTTTTCTGTTTTTGTTACCAACTTCC20.1480384900074019No Hit
GTCCCGGGGGACAGGATCAACCATAGGAAAGAAGATTCCT20.1480384900074019No Hit
GTCCCGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC20.1480384900074019No Hit
GTCCCGGGGGGGCTAGGGAGATGGCTCAGCAGTTAACAAC20.1480384900074019No Hit
GTCCCGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT20.1480384900074019No Hit
GTCCCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1480384900074019No Hit
GTCCCGGGGGGGGATGGTTTGATCTCACGGCTTTGCCTGA20.1480384900074019No Hit
GTCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG20.1480384900074019Illumina Single End Adapter 2 (97% over 34bp)
GTCCCGGGGGGGTTATTTGGTTAGGATTTTTCAAAATGAT20.1480384900074019No Hit
GTCCCGGGGGGGCCTATGTCGAGGGCCCCAAATTTACAAT20.1480384900074019No Hit
GTCCCGGGGCAGCAGTGTATCACCTTCAGAGGGGCATCGT20.1480384900074019No Hit
GTCCCGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT20.1480384900074019No Hit
GTCCCGGGGGCCGTCCTATAGATTTCAGCTATGGACAGAA20.1480384900074019No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCT20.1480384900074019No Hit
GTCCCGGGGGAGGAGAAGCTGATGATGCTCCAACATAAGA20.1480384900074019No Hit
GTCCCGGGGGGTTAGACTTTGGGCAGCACATGGTTGACCT20.1480384900074019No Hit
GTCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.1480384900074019No Hit
GTCCCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT20.1480384900074019No Hit
GTCCCGGGGCTGTCCCGGGGGCTTAAAACAAGCTACATGG20.1480384900074019No Hit
GTCCCGGGGAGGGGTTTTGTTGATAAATCAAGTCTTGGTT20.1480384900074019No Hit
GTCCCGGGGACAGGAATATGGGGTGTCTGGTTGCATAACA20.1480384900074019No Hit
GTCCCGGGGGAGATGAGATGACCTCCTTGTCAGAGTATGT20.1480384900074019No Hit
GTCCCGGGGGGTCCTTTATTTATAAGTGCTTATCCAGTAG20.1480384900074019No Hit
GTCCCGGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA20.1480384900074019No Hit
GTCCCGGGGACCTTAAATTCAAAACTCTATGAGCAGCCAA20.1480384900074019No Hit
GTCCCGGGGGACAGGATCAACCATAGGAAAGGAGATTCCT20.1480384900074019No Hit
GTCCCGGGGGGGATGGTGAAGAGCATTACAGGCAGTTACA20.1480384900074019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG402.0676453E-833.740745
CGGGGGA306.96821E-633.740745
CCCGGGG1250.033.740743
GTCCCGG1250.033.740741
CCGGGGG800.033.740744
CCGGGGC200.001991489733.740744
TCCCGGG1250.033.740742
CGGGGAT251.2008052E-433.7407385
CCGGGGA251.2008052E-433.7407384
Produced by FastQC (version 0.11.5)